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OfflineBaby_Hitler
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Can straw be pasteurized, dried, then rewetted & used later?
    #2003681 - 10/12/03 11:35 PM (13 years, 2 months ago)

If I were to pasteurize a bunch of straw and then dry it out under a fan and bag it up, could it be soaked in sterile water and used a few months, weeks or days later without repasteurizing?


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OfflineBaby_Hitler
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Re: Can straw be pasteurized, dried, then rewetted & used later? [Re: Baby_Hitler]
    #2005326 - 10/13/03 03:16 PM (13 years, 1 month ago)

Nobody wants to take a guess?


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InvisibleOldSpice
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Post deleted by Administrator [Re: Baby_Hitler]
    #2005345 - 10/13/03 03:23 PM (13 years, 1 month ago)



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InvisibleBi0TeK
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Re: Can straw be pasteurized, dried, then rewetted & used later? [Re: Baby_Hitler]
    #2005382 - 10/13/03 03:32 PM (13 years, 1 month ago)

Doesn't sound feasible to me but give it a go though. I use unorthodox methods often and have been pleasantly surprised by the outcome on occasions :smile: 


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OfflineBaby_Hitler
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Re: Can straw be pasteurized, dried, then rewetted & used later? [Re: OldSpice]
    #2005421 - 10/13/03 03:44 PM (13 years, 1 month ago)

If we were talkng about exposing sterile substrate I's agree, but pasteurized straw still has a considerable amount of life left in it. I don't think fanning would add that much to it.


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OfflineQuintessence
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Re: Can straw be pasteurized, dried, then rewetted & used later? *DELETED* [Re: Baby_Hitler]
    #2005564 - 10/13/03 04:31 PM (13 years, 1 month ago)

Post deleted by NuggetsTheShaker


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Edited by NuggetsTheShaker (10/13/03 04:35 PM)


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OfflineBaby_Hitler
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Re: Can straw be pasteurized, dried, then rewetted & used later? [Re: Quintessence]
    #2006492 - 10/13/03 10:31 PM (13 years, 1 month ago)

I guess that depends on how dirty the air is.


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InvisibleChampion des Champignons
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Re: Can straw be pasteurized, dried, then rewetted & used la [Re: Baby_Hitler]
    #2006761 - 10/13/03 11:51 PM (13 years, 1 month ago)

Quote:

Baby_Hitler said:
Nobody wants to take a guess?



I'd guess a pretty certain"NO"


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hmmm........


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Invisibledeanofmean
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Re: Can straw be pasteurized, dried, then rewetted & used la [Re: Champion des Champignons]
    #2006867 - 10/14/03 12:22 AM (13 years, 1 month ago)

maybe, if you dry it in a flow cabinet .


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Offlinethisone
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Re: Can straw be pasteurized, dried, then rewetted & used la [Re: deanofmean]
    #2006882 - 10/14/03 12:25 AM (13 years, 1 month ago)

It would probably still only stay sterile for two weeks since the pasteurazation is like a two week hold on contams and many microorganisms are still present.


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OfflineBaby_Hitler
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Re: Can straw be pasteurized, dried, then rewetted & used la [Re: thisone]
    #2007014 - 10/14/03 01:34 AM (13 years, 1 month ago)

But how much growth can happen without water?


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Offlinesirreal
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Re: Can straw be pasteurized, dried, then rewetted & used later? [Re: Baby_Hitler]
    #2008170 - 10/14/03 01:31 PM (13 years, 1 month ago)

Taken from the mushroom information section at the shroomery regarding bulk substrates:



How long will pasteurized bulk substrate last if I don?t inoculate it right away?

--------------------------------------------------------------------------------

By pasteurizing many bacteria and some molds still survive in the substrate. If you leave the pasteurized substrate uninoculated, it will "spoil" eventually.
The basic principle of spawning bulk substrate is the same regardless of the type of mushroom
or substrate. COLONIZATION MUST PROCEED AS RAPIDLY AS POSSIBLE TO PREVENT OTHER
ORGANISMS FROM BECOMING ESTABLISHED. Once the mushroom mycelium becomes dominant,
natural antibiotics secreted into the substrate inhibit competitors.
So, it's best not to store pasteurized substrate more than a few days, if at all.




Quote:

Baby_Hitler said:
If I were to pasteurize a bunch of straw and then dry it out under a fan and bag it up, could it be soaked in sterile water and used a few months, weeks or days later without repasteurizing?






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Edited by sirreal (10/14/03 01:34 PM)


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OfflineBaby_Hitler
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Re: Can straw be pasteurized, dried, then rewetted & used later? [Re: sirreal]
    #2008397 - 10/14/03 03:30 PM (13 years, 1 month ago)

I knew that already. That is referring to wet straw.


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Offlinesirreal
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Re: Can straw be pasteurized, dried, then rewetted & used later? [Re: Baby_Hitler]
    #2008837 - 10/14/03 06:05 PM (13 years, 1 month ago)

Quote:

Baby_Hitler said:
I knew that already. That is referring to wet straw.





Wouldn't that still apply even if you dried the straw out. What about the opportunity for endospores to develop?


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OfflineBaby_Hitler
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Re: Can straw be pasteurized, dried, then rewetted & used later? [Re: sirreal]
    #2008878 - 10/14/03 06:15 PM (13 years, 1 month ago)

Compare wet noodles, and dry noodles.


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InvisibleMrMaddHatter
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Re: Can straw be pasteurized, dried, then rewetted & used later? [Re: Baby_Hitler]
    #2008887 - 10/14/03 06:19 PM (13 years, 1 month ago)

I say no. I would think that after you dry it and bagged it for storing, that all kinds of "bad" spores would get all over the straw, then when you rewet it, those spores will grow. There MIGHT be a chance that if you used it a day or two after pasteurizing/drying that it could work, but as for storing for a few weeks or months then I have to say no. Then again we'd never have made it this far without experimentation.


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Offlinethisone
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Re: Can straw be pasteurized, dried, then rewetted & used later? [Re: Baby_Hitler]
    #2008888 - 10/14/03 06:20 PM (13 years, 1 month ago)

Drying the straw will not remove the bacteria. After pastuerization there are still numerous microorganisms in the straw. They will not thrive because it is wet, but they will remain present. After rewetting they will germinate very quickly as opposed to directly after pastuerization where they have about two weeks before bloom and hopefully by that time the mycelium network will have taken over.


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InvisibleMrMaddHatter
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Re: Can straw be pasteurized, dried, then rewetted & used later? [Re: thisone]
    #2008898 - 10/14/03 06:22 PM (13 years, 1 month ago)

Yea thats a good point too :thumbup:


--------------------
I hate to advocate drugs, alcohol, violence, or insanity to anyone, but they've always worked for me.
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It's always tea-time, and we've no time to wash the things between whiles.

Don't be a fool, this is a war on drugs. Act like. The government sure does!


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Offlinesirreal
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Re: Can straw be pasteurized, dried, then rewetted & used later? [Re: Baby_Hitler]
    #2010197 - 10/15/03 01:27 AM (13 years, 1 month ago)

Quote:

Baby_Hitler said:
Compare wet noodles, and dry noodles.





When you make noodles wet, you generally eat them. Not dry them out and rewet them weeks later.


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I may not always tell the truth, but atleast I'm honest
-----------

I see what everyone is saying. It is so hard to form an opinion when you see both sides so clearly!


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