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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
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1st Pan Cambo Sandose grow - Fail and Success update!
#19978053 - 05/12/14 07:32 PM (10 years, 8 months ago) |
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So, I figured my 1000th post should be a decent contributing one, so here's my first pan grow: Panaeolus cambodginiensis "Sandose".
I didn't take a pic of my brf substitute agar, but they were done in small (100-200 mL) standard sauce jars and swiped around 16th april.
BRF agar sub was a PITA to take decent wedges for grain master inocs, but once I got a piece jiggled out of the jar my rye berries stuck to it and the jars I shook like crazy looks like this today:

Jars was inocced with wedges 4/24, and I think the ones I didn't shake but rather just rolled the wedges into look like this:

As I don't have any manure yet I'm gonna try and g2g that first jar (all pics were of the same jar) into 6 new ones.
I also think I'll shake the ones that are halted as I dont see what there is to lose. Some posts say pans dont always recover after being shook, but there's only 1 way to find out if it'll work for me!
I've never worked with pan before, but I think the mycel looks healthy compared to other pan grows I've seen. That black little dot is a bit worrying though 
Update 11th July
First off, my coir trays failed. I must have measured up a qt too much water or something, the cvg was dripping wet, but I went all and spawned it anyway. Got 4 trays looking like this, only took a pic through the saran wrap:

The second set of g2g'd jars did much better, got my hands on some horse manure and spawned some trays on 6/21, all cased with cvg:

Yeah, my perlite was shit (not coarse enough) so I had to improvise some dub tubs. This next one had some slimey stuff on it but I cased it anyway and hoped for the best:

The first one was fruited on 7/7, and the second one on 7/8.
Found a great surprise today (7/11) on the first one after 4 days in fruiting:

The casing looked pretty dry so I thought I'd give the old skooled drip shield a go since I dont know how good my FAE is yet, I dont wanna risk leaving these pins wet and have them abort on me!


Update 12th July
So they've grown for another 20 hrs, looking great even though all of them are growing on the sides of the tray. I think I need to up my misting from once in the morning to twice a day.

I misted the fuck out of the casing layer with my glad wrap mist shield covering the fruits, but gave the fruits 2 light sprays from far away. I wanna get a wall to wall canopy some day so this mist shield is just a crutch I use until I feel more confident.
Update 14th July
Just a few more pics, some fruits had fallen over and felt really dry.

The rest is first flush on 2nd tray, with a cluster and a fat ass that will be cloned!

Oh and btw, I've been misting my fruits directly for the last 2-3 days. I haven't had any abort on me yet, but some get really cool color patterns on the hats from bruising though!


Update 15th July
This is pics from the second tray, the ice cream dome.

This guy is/was pretty cool:

After the first trays sporesplotion I've started picking them as soon as I see spores on their stem. Also a bit easier to make the old compact cam zoom in on the shrooms after they're picked.
Edited by spacechildo (07/15/14 06:01 PM)
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OH1Ogrown
Student



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Re: 1000th post - 1st Pan Cambo Sandose grow [Re: spacechildo]
#19978070 - 05/12/14 07:37 PM (10 years, 8 months ago) |
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Thanks for your contrubutions. I'll be
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cronicr



Registered: 08/07/11
Posts: 62,905
Loc: Van Isle
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Re: 1000th post - 1st Pan Cambo Sandose grow [Re: spacechildo]
#19978072 - 05/12/14 07:37 PM (10 years, 8 months ago) |
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those look healthy, great stuff to see space! glad your still plucking away at mycology
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 7 years, 4 months
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Re: 1000th post - 1st Pan Cambo Sandose grow [Re: spacechildo]
#19982748 - 05/13/14 05:32 PM (10 years, 8 months ago) |
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I have to correct something I said yesterday:
Quote:
spacechildo said: (..) and I think the ones I didn't shake but rather just rolled the wedges into look like this:

I have 4 jars looking like that, and when looking through my notes it seems those 4 are a month old jars that just didnt germinate anything after inoc. So I apparently re-inocced them
Anyway, I g2g'd the jars from first pics in op to 6 more, and all the grains broke off real easily. I did notice one marble sized chunk that didn't break off, and I marked the jar for caution. Had a real moment with some grains that was "impossible" to break up earlier and really wanna keep bacteria away this time.
Took some more pics of the other jars and noticed one more that's ready for g2g!

I hope it's all healthy, this jar is only 1 transfer away from the initial swipe.
Oh, and here is the jar I didn't shake like hell after dropping in the wedge. It's colonizing nicely but the other ones recovered from tiny spots all around the jar and will finish earlier.

Here's the last ones. I (hopefully) wont g2g any more jars, probably do some CVG trays until I stumble across some manure.
1:

2:

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nucleophile
international cowboy chemist


Registered: 04/28/13
Posts: 299
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Re: 1000th post - 1st Pan Cambo Sandose grow [Re: spacechildo]
#19982923 - 05/13/14 06:10 PM (10 years, 8 months ago) |
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I'll be watching with interest! I have a bunch of these ready too and just got manure as well.
I notice such a wild variation in the mycelium of different pans... I have 2 cyanescens (Australian and Hawaiian), tropicalis, and 2 cambos (Sandose and Goliath) and they all look somewhat different... the cambos are thick and the Australian is barely there. So strange.
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
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Re: 1000th post - 1st Pan Cambo Sandose grow [Re: nucleophile]
#19983922 - 05/13/14 09:26 PM (10 years, 8 months ago) |
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Thank you guys, I hope this will be a nice one!
Quote:
nucleophile said: I notice such a wild variation in the mycelium of different pans... I have 2 cyanescens (Australian and Hawaiian), tropicalis, and 2 cambos (Sandose and Goliath) and they all look somewhat different... the cambos are thick and the Australian is barely there. So strange.
I have no other first hand experience but this on pan cultivation, but the myc was really thick on brf agar sub and quite whispy on grains. It seems to grow thicker together quite fast after having colonizing some parts of the grains, in fact this almost grows a bit too fast all together for my lazy ass 
I just realized what that marbled sized unbreakable piece I probably mistook for bacteria was . it must've been the agar wedge I stuck in my master
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SimonJester


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Re: 1000th post - 1st Pan Cambo Sandose grow [Re: spacechildo]
#19984919 - 05/14/14 12:10 AM (10 years, 8 months ago) |
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Very interested in where this grow goes. I have a print that I'm terrified to do anything with.
Posting for future updates. 
Also, great 1000th post.
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spacechildo
proletarians rise up



Registered: 01/24/13
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Re: 1000th post - 1st Pan Cambo Sandose grow [Re: SimonJester] 1
#20016160 - 05/20/14 05:23 PM (10 years, 7 months ago) |
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quick update, the master jars that weren't shaken like crazy is working their slow way: Jar 3 I only dropped the wedge in and it's taking forever.
jar 1: jar 2: jar 3:
+ 
The jars from OP was g2g'ed to 6 more on 5/13 and looks eager to go: I'm considering g2g'ing a couple of these again as they grow so fast and I still haven't stepped into any manure as of yet..

Also g2g'd the second fastest master jar (out of 5 total) into only 4 new ones on 5/15. Didn't shake these after g2g, rather rolled the grains around trying to spread them. There was a good reason why only 4, just can't remember it anymore

I noticed a little greenish dark spot on 1 of these 4, tried to take a close up but both the cam and I suck at taking photos.

Keeping an eye on that one. Hopefully the next update will be of more spawned trays and less grain jars!
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 7 years, 4 months
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Re: 1000th post - 1st Pan Cambo Sandose grow [Re: spacechildo]
#20258462 - 07/11/14 03:34 PM (10 years, 6 months ago) |
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Wow! Has it really been this long? Anyway, here's an update!
Quote:
spacechildo said: Update 11th July
First off, my coir trays failed. I must have measured up a qt too much water or something, the cvg was dripping wet, but I went all and spawned it anyway. Got 4 trays looking like this, only took a pic through the saran wrap:

The second set of g2g'd jars did much better, got my hands on some horse manure and spawned some trays on 6/21, all cased with cvg:

Yeah, my perlite was shit (not coarse enough) so I had to improvise some dub tubs. This next one had some slimey stuff on it but I cased it anyway and hoped for the best:

The first one was fruited on 7/7, and the second one on 7/8.
Found a great surprise today (7/11) on the first one after 4 days in fruiting:

The casing looked pretty dry so I thought I'd give the old skooled drip shield a go since I dont know how good my FAE is yet, I dont wanna risk leaving these pins wet and have them abort on me!


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cronicr



Registered: 08/07/11
Posts: 62,905
Loc: Van Isle
Last seen: 8 minutes, 18 seconds
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Re: 1000th post - 1st Pan Cambo Sandose grow [Re: spacechildo]
#20259393 - 07/11/14 07:29 PM (10 years, 6 months ago) |
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-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 7 years, 4 months
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1st Pan Cambo Sandose grow [Re: cronicr]
#20262388 - 07/12/14 11:06 AM (10 years, 6 months ago) |
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So they've grown for another 20 hrs, looking great even though all of them are growing on the sides of the tray. I think I need to up my misting from once in the morning to twice a day.

How do you guys think about patching my coir+verm casing layer? its getting over grown by mycelium pretty fast now. I know RR advocate against late casing already fruited subs, but this is plain CVG which should be pretty contam resistent..
Edited by spacechildo (07/12/14 11:14 AM)
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MacMerdin
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Re: 1st Pan Cambo Sandose grow [Re: spacechildo]
#20263370 - 07/12/14 03:18 PM (10 years, 6 months ago) |
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I wouldn't worry about patching. Just keep the casing moist.
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spacechildo
proletarians rise up



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Re: 1st Pan Cambo Sandose grow [Re: MacMerdin]
#20263465 - 07/12/14 03:54 PM (10 years, 6 months ago) |
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Alright, thanks Mac! Always a bit nervous as a first timer, great to get some assurance by the seasoned!
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cronicr



Registered: 08/07/11
Posts: 62,905
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Re: 1st Pan Cambo Sandose grow [Re: spacechildo]
#20264370 - 07/12/14 08:08 PM (10 years, 6 months ago) |
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-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 7 years, 4 months
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Re: 1st Pan Cambo Sandose grow [Re: cronicr]
#20264676 - 07/12/14 09:38 PM (10 years, 6 months ago) |
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And merely 12 hrs later some of them have started sporulating!
It is now 36 hrs since I woke up and found the first pins, they've been growing like crazy in the 90F temp I have indoors 
I printed the 3 that have spores on their stems, took some pics right before and after: (I apologize in advance for my poor picture taking skills, they didn't quite turn out as I hoped)

Tried taking some upskirt photos but couldnt get my cam to focus on the correct shroom, and one that got hit a bit too hard by misting droplets and have bruised some:

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spacechildo
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Re: 1st Pan Cambo Sandose grow [Re: spacechildo]
#20272609 - 07/14/14 06:20 PM (10 years, 6 months ago) |
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Update 14th July
Just a few more pics, some fruits had fallen over and felt really dry.

These are first flush coming in on my 2nd tray, with a cluster and a fat ass that will be cloned!

Oh and btw, I've been misting my fruits directly for the last 2-3 days. I haven't had any abort on me yet, but some get really cool color patterns on the hats from bruising though!

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cArcace-x
YeAh!!


Registered: 10/17/13
Posts: 2,412
Loc: The Simulator
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Re: 1st Pan Cambo Sandose grow [Re: spacechildo]
#20273209 - 07/14/14 08:02 PM (10 years, 6 months ago) |
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Quote:
spacechildo said:
that's beautiful!!
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cronicr



Registered: 08/07/11
Posts: 62,905
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Re: 1st Pan Cambo Sandose grow [Re: cArcace-x]
#20273379 - 07/14/14 08:31 PM (10 years, 6 months ago) |
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yeah it's your pinset thats most sensitive to the mistings and you can see why
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 7 years, 4 months
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Re: 1st Pan Cambo Sandose grow [Re: cronicr]
#20273408 - 07/14/14 08:36 PM (10 years, 6 months ago) |
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So they abort before they even get to pop up and say hello 
I thought my 1st pinset was really crappy but most grew around the edges so I upped some on the misting. The 2nd tray looks a bit better, think the cv casing layer was a bit thicker and it got pins in the center as well.
Is there really that much of a difference to a knot or what they are when the misting kills them whether the water droplets come from a syringe or a gentle mist?
I thought the wet feet dry heads meant you could spray their feet a bit..
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cronicr



Registered: 08/07/11
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Re: 1st Pan Cambo Sandose grow [Re: spacechildo]
#20273428 - 07/14/14 08:41 PM (10 years, 6 months ago) |
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it's not the misting that kills them it's just you really need to make sure it dry's before doing so again, ii've fucked up my fair share before finding shroomery trying to find that happy medium but my caps always ended up looking like thatand i got much better results just waiting and watering the sub, there is no species of mushrooms in which you CAN'T mist it's just some seem more sensitive then others, cube pins will sometimes triple in size during a dunk where pans would simply abort
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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