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OfflineElektrolurch
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Psi. zapotecorum succesful cultivation (pics) * 2
    #539238 - 02/03/02 12:00 PM (22 years, 2 months ago)

Hi,

I was waiting since some time to post this results, because I wanted to go for sure. Now after BIO posted again about his Psi. zapotecurom pinning again, I thought the time would be suitable.

I managed to find some specimens of Psi. zapotecorum the last summer in the Zapotec sierra in Oaxaca (see Photo underneath).



I managed to gain pure cultres, by cloning of the above carpophores, and won also cultures from spores (and from other specimens not shown).

I inoculated sterilized straw within a jar with a clone-culture (from the left carpophore in the above picture) . After some time of incubation (room temperature 20 C) the micellium colonized the straw, which was used to spawn bigger amounts of pasteurized straw.

I filled a small tray with colonized straw. I let it recover for some days and cased it with a 3 cm thick soil/loam mixture, which was pasteurized and incubated for some weeks before application.
After a week of incubation the tray was putten into a fruiting chamber at 20 C with a 15 W light. The tray was covered with a thin foil to have a high humidity within it.

After some weeks it started to pin. When the fruitbodies achieved a size that was too big for the foil over the tray, I used a plastic bag (this within a terrarium that has already above 90 % humdity!) . Within two weeks one of the fruitbodies reached maturity and is sporulating, other are commeing. See the pictures above for a time series (about 2 weeks, taken about every two days, the last three ones are from the same day).

The interesting thing is that the cap has an abnormal form. After some days of research, and specially because the culture is a clone of the above specimen, I'm sure they are zapotecorum. Probably the conditions in the terrarium changed their appearance.

A very good sign that they are Psi. zapotecorum is the stem that is "floccose above to strongly scabrous-strigose at the base" (Guzman). Also the Lamellae (Gills) are specific, namely "sinuate or adnate". The fruitbodies show also a very soon bluing reaction when injured or touched, which tends to have "blackish violet tones"

Guzman also writes about the pileus (cap): "from conic or convex when young to subumbonate and papillate or convex to campanulate to convuluted or subconcave when old" The young specimens have the normal form, but the older ones are slightly more than subconcave. The first flush is always kind of different looking than the next ones (at least with cubensis, semperviva etc.).

The colour of the cap is somewhat light, but is probably due to the strong light. The specimens we found showed that the ones in the darker places had darker caps (brown) and the ones growing in places with light had more  a straw-like colour.

I'm still not completely sure shich mushroom it is. The next possible candidate would be Psi. argentipes (just kidding, argentipes is only known from Japan :wink:, but the description is very similar to the above fruitbodies).

Until now Psi. zapotecorum fits the best, I'm willing to know what you think about it. Thanx for your time,

Elektrolurch



















--------------------
"For all the time spent in that room
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InvisibleCLuB99
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Re: Psi. zapotecorum succesful cultivation (pics) [Re: Elektrolurch]
    #539314 - 02/03/02 01:34 PM (22 years, 2 months ago)

Increible! eres genia!:D
You're a incredibly skilled cultivator....
props to you elektro...so it's possible to cultivate those indoor....excellent!

Edited by CLuB99 (02/03/02 01:39 PM)

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InvisibleSporePimp
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Re: Psi. zapotecorum succesful cultivation (pics) [Re: CLuB99]
    #539324 - 02/03/02 01:49 PM (22 years, 2 months ago)

Very Impressive and excellent description, might have to dig out that culture. Thanks for sharing.

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OnlineWorkmanV
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Re: Psi. zapotecorum succesful cultivation (pics) [Re: Elektrolurch]
    #539508 - 02/03/02 06:09 PM (22 years, 2 months ago)

Simply amazing! I think the young specimens are a good likeness of the collection.


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OfflineRichard_D_James
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Re: Psi. zapotecorum succesful cultivation (pics) [Re: Workman]
    #539652 - 02/03/02 08:43 PM (22 years, 2 months ago)

beautiful fucking tits man!

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OfflineSThomas
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Re: Psi. zapotecorum succesful cultivation (pics) [Re: Richard_D_James]
    #539726 - 02/03/02 10:25 PM (22 years, 2 months ago)

Great job elektrolurch...wicked specimens, hope you figure out what they are...but certainly Psilos...cool.


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Invisiblemjshroomer
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Re: Psi. zapotecorum succesful cultivation (pics) [Re: Elektrolurch]
    #539872 - 02/04/02 01:20 AM (22 years, 2 months ago)

What an absoulutely amazing, astounding, stupendous, monumental, omnipotent, euphoric, psilophoric, historic display of mind food.

mj

"sing with rapture, dance like a dervish."

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Invisibleralphster44
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Re: Psi. zapotecorum succesful cultivation (pics) [Re: Elektrolurch]
    #540145 - 02/04/02 10:21 AM (22 years, 2 months ago)

Post deleted by administrator.


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OfflineElektrolurch
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Re: Psi. zapotecorum succesful cultivation (pics) [Re: Elektrolurch]
    #540239 - 02/04/02 12:07 PM (22 years, 2 months ago)

Thanx to all of you, especially because I learned over time a lot from this place and from you!

I'll keep you informed and updated...

Elektrolurch


mjshroomer,

your print is one of the next in the waiting que. It's already on agar, but they haven't germinated (soon they will). I hope it works and I can send you some back...


--------------------
"For all the time spent in that room
The doll's house, darkness, old perfume
And fairy stories held me high on
Clouds of sunlight floating by.", Pink Floyd '67

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OfflineElektrolurch
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Re: Psi. zapotecorum succesful cultivation (pics) [Re: Elektrolurch] * 1
    #544864 - 02/08/02 01:05 PM (22 years, 2 months ago)

Here is an update.

The biggest specimen in the above photos droped heavily spores and produced a beatiful brown purpureous sporeprint. I also got access to a microscope and shut some photos of the spores and from the pleurocystidia and cheilocystidia, probably also from the basidia. I don't have much experience microscoping mushrooms, so if someone has some hints or maybe a text on how to microscope mushroom lamenae, be welcome.

In the microscope pictures the doted line is about 10 micro-meter long, and between every point there is about 1 micro-meter. The photos without a line were taken without a scale.

Elektrolurch

Cap and sporeprint after 1 day:






MICROSCOPE PICTURES

Spores:









Cheilocystidia (?):









Basidia (?) :








Pleurocystidia (?) + a basidia on the left. The basidia seems to have all four spores on it :





--------------------
"For all the time spent in that room
The doll's house, darkness, old perfume
And fairy stories held me high on
Clouds of sunlight floating by.", Pink Floyd '67

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InvisibleGGreatOne234
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Re: Psi. zapotecorum succesful cultivation (pics) [Re: Elektrolurch]
    #546022 - 02/09/02 06:05 PM (22 years, 2 months ago)

woah!

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InvisibleWorld Spirit
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Re: Psi. zapotecorum succesful cultivation (pics) [Re: Elektrolurch]
    #546097 - 02/09/02 08:11 PM (22 years, 2 months ago)

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OfflineDinoMyc
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Re: Psi. zapotecorum succesful cultivation (pics) [Re: Elektrolurch]
    #546472 - 02/10/02 09:12 AM (22 years, 2 months ago)

brilliant, bravo.


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InvisibleIntelligentMind
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Re: Psi. zapotecorum succesful cultivation (pics) [Re: Elektrolurch]
    #3915130 - 03/14/05 03:46 AM (19 years, 1 month ago)

Hmm...found this deep in the threads.. Anyone ever cultivate this strain before? If so, shoot me over a print if you still have one.

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InvisibleHangnail
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Re: Psi. zapotecorum succesful cultivation (pics) [Re: IntelligentMind]
    #3915912 - 03/14/05 11:04 AM (19 years, 1 month ago)

not a cubensis"strain", different species


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Offlinetrig_grl
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Registered: 04/18/14
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Re: Psi. zapotecorum succesful cultivation (pics) [Re: Elektrolurch]
    #19936131 - 05/03/14 01:41 PM (9 years, 11 months ago)

This gives me hope for my 3rd try at this strain.
Im pretty sure first 2 times failed as the substrate
was too wet and just contaminated within 8 days.


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OfflineKuntryKiller
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Re: Psi. zapotecorum succesful cultivation (pics) [Re: trig_grl]
    #19955426 - 05/07/14 06:37 PM (9 years, 11 months ago)

:thumbup: congratulations

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