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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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nomadbrad
Oregrownian



Registered: 10/23/06
Posts: 1,160
Loc: Pacific NW
Last seen: 5 years, 4 months
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I saw that print in person and I'd say it's the thickest print I've ever seen. Thicker then a Gymnopilus print even!
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maynardjameskeenan
The white stipes



Registered: 11/11/10
Posts: 16,391
Loc: 'Merica
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Re: Maynard's random picture thread [Re: Byrain]
#19913422 - 04/28/14 07:05 PM (9 years, 9 months ago) |
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Quote:
Byrain said: I'm going with P. olivaceus, that unknown P. olivaceus lookalike with sulphidia from the PNW, or P. fimicola. Lets see the micro!
Hey so my microscope skills fucking suck, I don't know what I'm doing, what I'm looking for or where to look for it. All I can make out are spores and translucent blobs. I'm ashamed to post any mircographs.
-------------------- May you be filled with loving kindness. May you be well. May you be peaceful and at ease. May you be happy. AMU Q&A
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Joust
Mycotographer




Registered: 10/13/11
Posts: 13,392
Loc: WA
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Quote:
maynardjameskeenan said:
Quote:
Joust said: WAIT THE FUCK!?
That spore print looks thick!!.. like 3mm deep.. am i seeing that right?!
Yeah, it looks to be that way. I kinda thought it looks like a graphics-equalizer or something.

joust will be back after he gathers his head parts...
though really, great macro, i wish you had a cheap ring flash =)
-------------------- ~~~~~~***Psilocybin Mushrooms***~~~~~~ _________A Practical Guide To Psilocybin Mushrooms_________ "Think about the species, not your scale". -NeoSporen "Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira
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The Lightning
Mycology Enthusiast


Registered: 09/06/11
Posts: 3,889
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Dude, pull your s*** together AND make a spore slide!
Take a spore print from your next mushroom collection by placing a cap on-top of a microscope slide.
Edited by The Lightning (04/29/14 06:01 AM)
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maynardjameskeenan
The white stipes



Registered: 11/11/10
Posts: 16,391
Loc: 'Merica
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-------------------- May you be filled with loving kindness. May you be well. May you be peaceful and at ease. May you be happy. AMU Q&A
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The Lightning
Mycology Enthusiast


Registered: 09/06/11
Posts: 3,889
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There you go! Now keep studying and keep calm!
Make adjustments as desired.
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maynardjameskeenan
The white stipes



Registered: 11/11/10
Posts: 16,391
Loc: 'Merica
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I have a hard time differentiating other microscopic structures (besides spores), especially when I crush mouth it- it looks like a whole bunch of clear cells with some spores scattered about. How do you know what to look for and where? I could spend hours on one slide and still have nothing to show for it.
-------------------- May you be filled with loving kindness. May you be well. May you be peaceful and at ease. May you be happy. AMU Q&A
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The Lightning
Mycology Enthusiast


Registered: 09/06/11
Posts: 3,889
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Nothing will be lost by studying a few extra hours. The following micro-structures will become obvious soon enough if you apply even the minimum effort:
1. Spores 2. Spores on Basidia (Basidium) 3. Cheilocystidida (cells along the true gill edge) 4. Pleurocystidia (cells along the "false" gill edge)
Make a Preparate cut-out (a square cut out of an individual gill, including the cap area).
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maynardjameskeenan
The white stipes



Registered: 11/11/10
Posts: 16,391
Loc: 'Merica
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What are sulphidia and where would I find them? I think if I had stain it would make things easier, I can barely see any delineation between where the cells are sometimes.
-------------------- May you be filled with loving kindness. May you be well. May you be peaceful and at ease. May you be happy. AMU Q&A
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Joust
Mycotographer




Registered: 10/13/11
Posts: 13,392
Loc: WA
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Quote:
maynardjameskeenan said: What are sulphidia and where would I find them? I think if I had stain it would make things easier, I can barely see any delineation between where the cells are sometimes.
Dont worry bout this for now, The more you scope the easier it is to know what you are looking at. Just keep at it. A scope that has good clarity and time is your best friend, as well the people here
-------------------- ~~~~~~***Psilocybin Mushrooms***~~~~~~ _________A Practical Guide To Psilocybin Mushrooms_________ "Think about the species, not your scale". -NeoSporen "Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira
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The Lightning
Mycology Enthusiast


Registered: 09/06/11
Posts: 3,889
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Quote:
maynardjameskeenan said: What are sulphidia and where would I find them? I think if I had stain it would make things easier, I can barely see any delineation between where the cells are sometimes.
Here's an updated definition of sulphidia:
Sulphidium (pl., sulphidia): Pleurocystidium that have a yellowish coloration (similar to that of of a chrysocystidium). So far this term has been used extremely infrequently. E. Gerhardt, in his monograph on Panaeolus (Taxonomische revision der gattungen Panaeolus und Panaeolina, Bibliotheca Botanica 47, 1996), defines sulphidia as follows (translation from German): "There are pleurocystidia, so far called chrysocystidia but not matching this definition as they do not stain yellow with KOH (unlike Stropharia and Hypholoma), but they are yellowish from the very beginning or keep uncolored. They become a beautiful red wine in sulphovanillin (sulfo-vanillin, sulpho-vanillin), consequently they are called sulphidia." The stain patent blue may be useful as an alternative to sulphovanillin.
Sulphidia may be rare cells found in very limited genera. Panaeolus is the only genus I currently know of that produce sulphidia as definied above.
When you perform microscopy focus on one cell form at a time (ie spores first, then cheilocystidia, etc).
I would get good at viewing spores and cheilocystidia first.
I would only move onto to crush mounts after first getting perfectly calm and comfortable with viewing cells without crushing (smashing, squashing) them.
Learn how to make a preparate cut-out as seen here. You can use CTRL+F to search for the phrase "preparate cut-out".
I feel bad for telling you to pull your shit together last night.
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Byrain

Registered: 01/07/10
Posts: 9,664
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I don't think I would call them cystidia and they aren't really similar to chrysocystidia in that they don't change colors in KOH.
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maynardjameskeenan
The white stipes



Registered: 11/11/10
Posts: 16,391
Loc: 'Merica
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Quote:
The Lightning said:
Quote:
maynardjameskeenan said: What are sulphidia and where would I find them? I think if I had stain it would make things easier, I can barely see any delineation between where the cells are sometimes.
Here's an updated definition of sulphidia:
Sulphidium (pl., sulphidia): Pleurocystidium that have a yellowish coloration (similar to that of of a chrysocystidium). So far this term has been used extremely infrequently. E. Gerhardt, in his monograph on Panaeolus (Taxonomische revision der gattungen Panaeolus und Panaeolina, Bibliotheca Botanica 47, 1996), defines sulphidia as follows (translation from German): "There are pleurocystidia, so far called chrysocystidia but not matching this definition as they do not stain yellow with KOH (unlike Stropharia and Hypholoma), but they are yellowish from the very beginning or keep uncolored. They become a beautiful red wine in sulphovanillin (sulfo-vanillin, sulpho-vanillin), consequently they are called sulphidia." The stain patent blue may be useful as an alternative to sulphovanillin.
Sulphidia may be rare cells found in very limited genera. Panaeolus is the only genus I currently know of that produce sulphidia as definied above.
When you perform microscopy focus on one cell form at a time (ie spores first, then cheilocystidia, etc).
I would get good at viewing spores and cheilocystidia first.
I would only move onto to crush mounts after first getting perfectly calm and comfortable with viewing cells without crushing (smashing, squashing) them.
Learn how to make a preparate cut-out as seen here. You can use CTRL+F to search for the phrase "preparate cut-out".
I feel bad for telling you to pull your shit together last night.
Thanks for your help, sometimes I need someone to tell me to get my shit together  I'll work on them more tonight once the kids go to bed, being solely responsible for three kids- one of them a toddlers and microscopy don't go together very well. Precision instruments and small kids don't mix.
-------------------- May you be filled with loving kindness. May you be well. May you be peaceful and at ease. May you be happy. AMU Q&A
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Joust
Mycotographer




Registered: 10/13/11
Posts: 13,392
Loc: WA
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Quote:
maynardjameskeenan said:
Quote:
The Lightning said:
Quote:
maynardjameskeenan said: What are sulphidia and where would I find them? I think if I had stain it would make things easier, I can barely see any delineation between where the cells are sometimes.
Here's an updated definition of sulphidia:
Sulphidium (pl., sulphidia): Pleurocystidium that have a yellowish coloration (similar to that of of a chrysocystidium). So far this term has been used extremely infrequently. E. Gerhardt, in his monograph on Panaeolus (Taxonomische revision der gattungen Panaeolus und Panaeolina, Bibliotheca Botanica 47, 1996), defines sulphidia as follows (translation from German): "There are pleurocystidia, so far called chrysocystidia but not matching this definition as they do not stain yellow with KOH (unlike Stropharia and Hypholoma), but they are yellowish from the very beginning or keep uncolored. They become a beautiful red wine in sulphovanillin (sulfo-vanillin, sulpho-vanillin), consequently they are called sulphidia." The stain patent blue may be useful as an alternative to sulphovanillin.
Sulphidia may be rare cells found in very limited genera. Panaeolus is the only genus I currently know of that produce sulphidia as definied above.
When you perform microscopy focus on one cell form at a time (ie spores first, then cheilocystidia, etc).
I would get good at viewing spores and cheilocystidia first.
I would only move onto to crush mounts after first getting perfectly calm and comfortable with viewing cells without crushing (smashing, squashing) them.
Learn how to make a preparate cut-out as seen here. You can use CTRL+F to search for the phrase "preparate cut-out".
I feel bad for telling you to pull your shit together last night.
Thanks for your help, sometimes I need someone to tell me to get my shit together  I'll work on them more tonight once the kids go to bed, being solely responsible for three kids- one of them a toddlers and microscopy don't go together very well. Precision instruments and small kids don't mix.
MJK, you and byrain are the people who usually tell me to get my shit together, and its great.
-------------------- ~~~~~~***Psilocybin Mushrooms***~~~~~~ _________A Practical Guide To Psilocybin Mushrooms_________ "Think about the species, not your scale". -NeoSporen "Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira
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The Lightning
Mycology Enthusiast


Registered: 09/06/11
Posts: 3,889
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Re: Maynard's random picture thread [Re: Byrain]
#19918270 - 04/29/14 06:59 PM (9 years, 9 months ago) |
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Quote:
Byrain said: I don't think I would call them cystidia and they aren't really similar to chrysocystidia in that they don't change colors in KOH.
Byrain, you probably don't need anyone telling you this, but you of all people (being someone who studies Panaeolus species) should go beyond Gerhardt's work and prove what sulphidia ARE and what they ARE NOT.
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maynardjameskeenan
The white stipes



Registered: 11/11/10
Posts: 16,391
Loc: 'Merica
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Our derp cat

I found this big moth on an empty hummingbird feeder last night.


Cyan pot



I had some ovoid spawn in a rosemary plant but when it got cold this winter I think it died and the mycelium started eating it.

Foes




Strawberries

Our herb and garden bed

I have nothing else to show you at the moment, thanks for viewing.
-------------------- May you be filled with loving kindness. May you be well. May you be peaceful and at ease. May you be happy. AMU Q&A
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domesticgnome

Registered: 04/22/11
Posts: 3,079
Loc: For me to know and you to find...
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That moth is killer
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Joust
Mycotographer




Registered: 10/13/11
Posts: 13,392
Loc: WA
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I was expecting Herbs... not herbs Awesome cyan mulch, do you have it covered or are the rhizos just pokig out for fun?
-------------------- ~~~~~~***Psilocybin Mushrooms***~~~~~~ _________A Practical Guide To Psilocybin Mushrooms_________ "Think about the species, not your scale". -NeoSporen "Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira
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maynardjameskeenan
The white stipes



Registered: 11/11/10
Posts: 16,391
Loc: 'Merica
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Re: Maynard's random picture thread [Re: Joust]
#19951604 - 05/06/14 09:49 PM (9 years, 9 months ago) |
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I keep it covered with a scrap of plywood, mycelium doesn't seem to like the light.
-------------------- May you be filled with loving kindness. May you be well. May you be peaceful and at ease. May you be happy. AMU Q&A
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maynardjameskeenan
The white stipes



Registered: 11/11/10
Posts: 16,391
Loc: 'Merica
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Hohenbuehelia petaloides


Leratiomyces percevalii



Agrocybe





maybe Lycogala epidendrum

Cystoderma adnatifolium? 




April shower bring May flowers





-------------------- May you be filled with loving kindness. May you be well. May you be peaceful and at ease. May you be happy. AMU Q&A
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