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OfflineJunior Fungus
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Registered: 09/19/11
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Slow Colonization with MS in WBS
    #19919153 - 04/29/14 10:06 PM (10 years, 8 months ago)

I have many jars which I inoculated with an MS. Some are BRF/verm and others are WBS/gypsum. Both are very slow at germinating and colonizing.

Here are the potential mistakes I can think of:

1- I wasn't sterile enough and my SAB is crappy. Which would lead to minor contams which the mycelium overcomes, but only after a long period of time. (I doubt that would happen, but can it?)

2- The ambient temperature is between 65F and 72F.

3- I am letting the jars colonize on my dresser, in my room, close enough to my door. (too much movement in the surrounding air?)

4- Jars have 4 needle-size holes each, covered in masking tape. (not enough GE?)

5- Jars get a little over 12h indirect sunlight on average.

6- My thermometer may be a dirty liar about never going under 65F... maybe it's actually colder than that?

I'm using Golden teacher in the BRF, which has been a slow colonizer for me in the past, no surprise there. For the faster colonizing grain, however, I used a faster colonizing strain, KSSS. That being said, why in the world would the grain not colonize fast? Especially compared to the slower BRF/GT combo.

So what do you guys think it is?


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My Grow Logs:    Psychoactives:mushroom2:      Edibles & Medicinals 

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Invisiblemaddchef
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Registered: 09/04/09
Posts: 5,602
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Re: Slow Colonization with MS in WBS [Re: Junior Fungus]
    #19919159 - 04/29/14 10:10 PM (10 years, 8 months ago)

The masking tape. Use micropore tape instead. Your jars need gas Exchange.


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In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:

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OfflinecronicrMFacebook
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Re: Slow Colonization with MS in WBS [Re: maddchef]
    #19919186 - 04/29/14 10:20 PM (10 years, 8 months ago)

yep, remove the tape all together and dont even bother with the micropore:thumbup:


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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Invisiblemaddchef
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Re: Slow Colonization with MS in WBS [Re: cronicr]
    #19919194 - 04/29/14 10:21 PM (10 years, 8 months ago)

Gotta have a filter on the wbs jars


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:

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OfflinecronicrMFacebook
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Re: Slow Colonization with MS in WBS [Re: maddchef]
    #19919199 - 04/29/14 10:22 PM (10 years, 8 months ago)

Quote:

maddchef said:
Gotta have a filter on the wbs jars



that 2


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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OfflineMonkeyJesusFresco
am i suspended in agar?
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Registered: 10/09/12
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Re: Slow Colonization with MS in WBS [Re: Junior Fungus] * 1
    #19919251 - 04/29/14 10:42 PM (10 years, 8 months ago)

1- I wasn't sterile enough and my SAB is crappy. Which would lead to minor contams which the mycelium overcomes, but only after a long period of time. (I doubt that would happen, but can it?)

Possible but highly doubtful, even the crappiest SAB can yield surprisingly successful results; in the
future blast it with Lysol and wait an hour, this will allow any airborne contams to settle and it will also just make you feel better 


2- The ambient temperature is between 65F and 72F.

Slower colonization happens in that temperature range, but not slow enough to post a thread on the shroomery about

3- I am letting the jars colonize on my dresser, in my room, close enough to my door. (too much movement in the surrounding air?)

Not an issue

4- Jars have 4 needle-size holes each, covered in masking tape. (not enough GE?)

For the cakes, no need for tape.

5- Jars get a little over 12h indirect sunlight on average.

We've all heard "something something something mushrooms and light" but it turns out, light whatever, it doesn't matter, let 'em have it, they love it!

6- My thermometer may be a dirty liar about never going under 65F... maybe it's actually colder than that?

Check that anyways.

I'm using Golden teacher in the BRF, which has been a slow colonizer for me in the past, no surprise there. For the faster colonizing grain, however, I used a faster colonizing strain, KSSS. That being said, why in the world would the grain not colonize fast? Especially compared to the slower BRF/GT combo.

Sniff around the filter of your grain jars and the holes of your cakes and see if there's a stinky smell, could be some bacteria :shrug: Ya know, it really could be the temp in your room, is it cold where you live?


So what do you guys think it is?

What I think is in bold.


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LAGM v 2.024
- endo cabendo

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OfflineJunior Fungus
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Registered: 09/19/11
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Re: Slow Colonization with MS in WBS [Re: MonkeyJesusFresco]
    #19919311 - 04/29/14 11:00 PM (10 years, 8 months ago)

I was a little lazy and pressed for time when I was preparing these jars, so I really just wished for the best. I treated my wbs jars just like a noob would treat a brf jar: with a hole in each corner. I have no choice but to use micropore tape on them at this point, right? I tried Shopper's Drug Mart and couldn't find any, so I used masking tape. I seem to recall thinking of masking tape from following a tek, so I figured it would breathe... does masking tape not breathe enough for GE?

The temperature here, outside, has been low, as in around 15C, which is 59F. My room is heated a bit, but we try to keep our power consumption here low.

Monkey, you mentioned tape being unnecessary for cakes, but what do you think about my grain jar situation?


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My Grow Logs:    Psychoactives:mushroom2:      Edibles & Medicinals 

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OfflineMonkeyJesusFresco
am i suspended in agar?
Male


Registered: 10/09/12
Posts: 3,308
Loc: South East USA Flag
Last seen: 24 days, 15 hours
Re: Slow Colonization with MS in WBS [Re: Junior Fungus]
    #19920486 - 04/30/14 06:24 AM (10 years, 8 months ago)

yeah, the tape is unnecessary for the cakes because there's a dry layer of vermiculite on top that acts as a filter to keep contams away from the 'meat' of your cake;

on grain jars, it's best to have a poly-fil filter or tyvex filter...

Quote:

does masking tape not breathe enough for GE?




ah, I think it's obvious that it's not

find some micropore tape or poly-fil and search around on here

best of luck, if I wasn't in a hurry I'd link you to some teks :crazy:


--------------------
LAGM v 2.024
- endo cabendo

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OfflineJunior Fungus
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Registered: 09/19/11
Posts: 352
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Re: Slow Colonization with MS in WBS [Re: MonkeyJesusFresco]
    #19934837 - 05/03/14 03:36 AM (10 years, 8 months ago)



I'm fine, I guess I just got impatient with this 65F business on my dresser.  Here's the germination on WBS.  Also colonizing agar to brf transfer of a salvaged GT rhizo clone in ziplock mycobag. MS to cakes also GT. I may have a great strain isolated! I'm expecting strong fruiting capabilities, because the bag is all Rhizo and its spreading fast even in BRF.

I'm contemplating propagating this clone mycobag to some WBS like a g2g. That would be to preserve and propagate the good genetics.  Think that would  work well?
>BRF mycelium transfer to WBS.


   


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My Grow Logs:    Psychoactives:mushroom2:      Edibles & Medicinals 

Edited by Junior Fungus (05/03/14 03:56 AM)

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Invisibleaudiosnipez
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Registered: 06/26/18
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Re: Slow Colonization with MS in WBS [Re: Junior Fungus]
    #25301508 - 06/30/18 06:55 PM (6 years, 6 months ago)

Polyfil caps

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