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OfflinePsilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 3 months
Re: Whats the deal with FastFred's Media Cookbook? [Re: 36fuckin5]
    #19908607 - 04/27/14 05:54 PM (9 years, 10 months ago)

Quote:

36fuckin5 said:

Try it with different isolates. Yours just happens to like 6%, that's not a general rule.




Mine is the only thread I've seen on this subject with pictures; all the rest just seems to point back at a few things announced as fact in a book by Oeric and Terrence McKenna which is largely considered outdated (especially in its agar section).  You can do the experiment with some of your isolates, if you want.  Let us know how it goes.  :asianofapproval:


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.

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InvisiblePastywhyteMDiscord
Say hello to my little friend
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Registered: 09/15/12
Posts: 37,830
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Re: Whats the deal with FastFred's Media Cookbook? [Re: 36fuckin5]
    #19908611 - 04/27/14 05:55 PM (9 years, 10 months ago)

Quote:

36fuckin5 said:
Lower nutrient content is more conducive to faster growth because they myc is searching for food. Yeah it'll be more rhizo too but that doesn't matter.

Quote:

van der griegen said:
I mean I tested the same isolate on 0%, 2%, 4%, 6% and 8% starch to see which would grow the fastest.  There wasn't a hell of a lot of variation, but 6% was the winner.




Try it with different isolates. Yours just happens to like 6%, that's not a general rule.




I'm also sure some prefer 2% some may like 8%. Then the general statement should be; Start somewhere between 1-8% nutes and experiment to see what works for your cultures. Rather than

Quote:

36fuckin5 said:
Ok, so you don't want 3-4% nutrient content in your agar. It'd be 3% tops, I usually shoot for 2%.




But I guess we don't actually want people to test things for themselves :shrug:

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Invisible36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,083
Loc: Diving into Mystical Territori...
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Re: Whats the deal with FastFred's Media Cookbook? [Re: Pastywhyte]
    #19908770 - 04/27/14 06:38 PM (9 years, 10 months ago)

Quote:

Pastywhyte said:
But I guess we don't actually want people to test things for themselves :shrug:




I don't wanna encourage people to waste their time when the tests have been done.

Stop looking at amateurs on forums and read some real microbiology books.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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OfflinePsilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 3 months
Re: Whats the deal with FastFred's Media Cookbook? [Re: 36fuckin5]
    #19908777 - 04/27/14 06:42 PM (9 years, 10 months ago)

Quote:

36fuckin5 said:
Quote:

Pastywhyte said:
But I guess we don't actually want people to test things for themselves :shrug:




I don't wanna encourage people to waste their time when the tests have been done.

Stop looking at amateurs on forums and read some real microbiology books.




So...those real microbiology books do a lot of experimentation on cubensis, do they?  :rofl:

Give me sources, then.

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OfflinecronicrFacebook
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Re: Whats the deal with FastFred's Media Cookbook? [Re: Psilicon] * 1
    #19908782 - 04/27/14 06:44 PM (9 years, 10 months ago)

i'm just posting because good info tends to come from these "discussions":cool:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

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OfflineTheApprentice
back at it
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Registered: 09/25/11
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Re: Whats the deal with FastFred's Media Cookbook? [Re: PsilocyBen17]
    #19908832 - 04/27/14 06:58 PM (9 years, 10 months ago)

Quote:

PsilocyBen17 said:
Whats the deal with FastFred's Media Cookbook?

http://www.shroomery.org/9393/FastFreds-Media-Cookbook

Is there more to it? Cuz I can't seem to find the info.

I have some agar-agar and want to try a no pour recipe.





Yo, I have a great tek in my.sig


--------------------


RR Videos -Best $9 Ever Spent *
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OfflinecronicrFacebook
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Re: Whats the deal with FastFred's Media Cookbook? [Re: TheApprentice]
    #19908846 - 04/27/14 07:01 PM (9 years, 10 months ago)

i don't mind no pours, i do agree petri's are better suited but if ya got some jars kicking around y not, i don't have the issues 36 speaks of but i dont us a scalpel or work with my jar flat either, i turn it on it's side and use a cutical pusher for cuts, blades are hard to work with with the rims of jars but you get use to it and develop a good technique like you would with any hurdle


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor

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InvisiblePastywhyteMDiscord
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Registered: 09/15/12
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Re: Whats the deal with FastFred's Media Cookbook? [Re: Psilicon]
    #19908865 - 04/27/14 07:05 PM (9 years, 10 months ago)

Quote:

36fuckin5 said:
Quote:

Pastywhyte said:
But I guess we don't actually want people to test things for themselves :shrug:




I don't wanna encourage people to waste their time when the tests have been done.

Stop looking at amateurs on forums and read some real microbiology books.




When is it a waste of time to try and learn something? If we never questioned "common knowledge" or "scientifically accepted knowledge" or if amateurs never tried to prove something, we would still think the world is flat :lol: Sure some things that people try are pointless. I would never advocate using urine as a nutrition source.

Besides, for me anyway, this is not a business I am running. I work 70 hrs a week to pay my bills. This is a hobby as far as I'm concerned. I like to push the boundaries of things. As for "real sources" of information, even things that were considered the gospel 20 years ago, are laughable today. You think that everything that is known regarding mycology has been discovered and the book is closed on every possible topic? Fact is that van der grigens little waste of time proved that for at least that particular strain he was working with, 2% was not nearly as effective as the 4% the 6% or the 8%. Which means that we don't know as much as we think. So if you have a person working with a culture that is not doing well on 2% your just gonna tell him to pitch it rather than suggest a different concentration? Who is wasting who's time now?

I already said this in vdg's OP on the subject but, I would like to see more experiments on this particular subject as I feel that the "officially proven conclusions" are far from the final word on this. If you care to replicate the experiment 36 and maybe post a pic :jawdrop: I would be more interested to see your results rather than just hearing your anecdotes :shrug:

Quote:

van der griegen said:

So...those real microbiology books do a lot of experimentation on cubensis, do they?  :rofl:

Give me sources, then.




:whathesaid:

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Invisible36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,083
Loc: Diving into Mystical Territori...
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Re: Whats the deal with FastFred's Media Cookbook? [Re: Pastywhyte]
    #19910084 - 04/28/14 12:50 AM (9 years, 10 months ago)



--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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OfflinePsilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 3 months
Re: Whats the deal with FastFred's Media Cookbook? [Re: 36fuckin5]
    #19910207 - 04/28/14 01:44 AM (9 years, 10 months ago)

Quote:

36fuckin5 said:
http://scholar.google.com/scholar?hl=en&q=agar+media+saphrophytic+fungi&btnG=&as_sdt=1%2C49&as_sdtp=

There's your source. You can sift through it.




Why?  You clearly haven't.  :rofl: 

If you had, you might have found this article, which would have led you to this article where they use 0.2% mea (1g/500g) for isolating basidiomycetes.  If you corrected the spelling a bit (it's "saprophytic", not "saphrophytic"), you'd find out a bit about Sabouraud's agar, which has a dextrose content of 4%.  The truth is, while 2-3% is a good range for a catch-all agar, there are a wide variety of agar recipes, each of which calls for its own concentration of nutrients.  None of them that I found were specific to agaricales, so I did my own research.  I'm sorry if it offends you that I found something other than the orthodox, but if you don't like it feel free to prove me wrong by either actually giving me a source or doing the damn experiment yourself.


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.

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Invisible36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,083
Loc: Diving into Mystical Territori...
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Re: Whats the deal with FastFred's Media Cookbook? [Re: Psilicon]
    #19910223 - 04/28/14 01:54 AM (9 years, 10 months ago)

Read more.

And I'm not offended. I've just read through all that shit before. When I'm at my friend's house who is in school, I pick up his microbiology books and read. I'm really that kind of nerd.

You got me on the typo. Good job, I guess? It's autocorrected anyway.

I've done the experiments myself. Many times over. I just don't document my felonies usually. Doesn't seem like a good idea.

If you look through my album, I have very few pics. And I still earned a TC tag from my words.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

Extras: Filter Print Post Top
OfflinePsilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 3 months
Re: Whats the deal with FastFred's Media Cookbook? [Re: 36fuckin5]
    #19910285 - 04/28/14 02:35 AM (9 years, 10 months ago)

You have sources, but you can't find them.  You did the experiment (many times, no less), but you don't want to show it.  Uh-huh.  Did you use your words to report on it then, or were you just waiting for this moment to spring it on us? 

:nomoreinternet:


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.

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Invisible36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,083
Loc: Diving into Mystical Territori...
Trusted Cultivator
Re: Whats the deal with FastFred's Media Cookbook? [Re: Psilicon]
    #19910350 - 04/28/14 03:24 AM (9 years, 10 months ago)

10 years from now you cite me all the sources you've ever read.

I feel no need to prove myself. I haven't found a single species yet that I can't grow. Again, no I don't document my felonies. Some of us don't play around.

Take it or leave it. I'll continue to get crazy canopies applying what I know.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

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OfflineMonkeyJesusFresco
am i suspended in agar?
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Re: Whats the deal with FastFred's Media Cookbook? [Re: 36fuckin5]
    #19910893 - 04/28/14 09:06 AM (9 years, 10 months ago)

anywayz,

as for the "2%,4%,6%" experiment, ya don't wanna use a pre-isolated culture for that, it'll just perform best on whichever it wants; I wanna see it done starting with a multi-spore culture;

start on 2% with a multispore, transfer the leading edge to 4% and continuing doing the same, transferring the most "aggressive" to 6% then to so on to 8%; I guess, that manner of strain isolation would eventually give you the overall best performer? :shrug: but best performer on what? agar??

side-note:

I'm feeling argumentative myself...

Quote:

:regularshroom: :regularshroom: :regularshroom: :regularshroom: :regularshroom:
Registered: Aug 11 2003
Posts: 6,080
"Trusted Cultivator"





nah...think I'll head over to the thread where the guy's building a condominium-style fruiting chamber with central heating and air(and room for a pony) for his one pinning pf-cake and get into an argument with him...


--------------------
LAGM v 2.024
- endo cabendo

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