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OfflineCubieman420
Sharing in thegroove
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Registered: 03/20/02
Posts: 2,693
Loc: Midwest
Last seen: 10 years, 3 months
Do colinizing jars need fresh air?
    #1981154 - 10/05/03 11:49 AM (20 years, 5 months ago)

9 days ago I innoculated 6 1/2 pint jars with 15cc's of GT solution, as of today there is not one speck of mycelium at all. To keep the jars in a sterile enviroment I put them in a shoe-boxed sized STERILE tupperware type thing then put this box into a larger storage container also sterilized, then a heat pad on low is under it. My question is...could the fact that no fresh air is getting to these jars attribute to the slow/no growth? Also, would it be a good idea to inject more spores into the jars after few days if nothing happens? ...I am only fucking with dark syringes from now on, regardless of popular belief I have had bad times with clear syringes. peace.


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"...now waters run free, no more fish in the sea..."
1983-2004

Edited by Cubieman420 (10/05/03 11:50 AM)

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InvisibleMagash
Da Bud Guru
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Registered: 07/25/02
Posts: 5,876
Loc: Near Hilo
Trusted Cultivator
Re: Do colinizing jars need fresh air? [Re: Cubieman420]
    #1981166 - 10/05/03 11:55 AM (20 years, 5 months ago)

Same here that is why I tell everybody to make their own.





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All creatures tremble when faced with violence. All creatures fear death, all love life. If we can only see ourselves in others, then how could we possibly hurt another creature?


:growingweed: Join us at the Growery! :growingweed:

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Invisiblehoushroom
shroom cowboy

Registered: 05/07/03
Posts: 678
Loc: space city
Re: Do colinizing jars need fresh air? [Re: Cubieman420]
    #1981186 - 10/05/03 12:00 PM (20 years, 5 months ago)

to begin germination i don't believe the jars need fresh air.  i've found that as it nears 100% completion i have to loosen the lids and turn them upside down to get them to finish.  but in your case i think you're allright. 

what i'm worried about is the heating pad.  those things, even on low, can get hot.  if it's at all possible i'd suggest getting a fish tank heater and two rubbermaids and make a incubator.  it's not that expensive or hard to do and it's worth it.  good luck :wink: 

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InvisibleMagash
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Registered: 07/25/02
Posts: 5,876
Loc: Near Hilo
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Re: Do colinizing jars need fresh air? [Re: houshroom]
    #1981283 - 10/05/03 12:46 PM (20 years, 5 months ago)

I fully agree with ya on that.



I like to put a little pump in there to stir the water. keeps things from heating from the bottom up.



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All creatures tremble when faced with violence. All creatures fear death, all love life. If we can only see ourselves in others, then how could we possibly hurt another creature?


:growingweed: Join us at the Growery! :growingweed:

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InvisibleHertyar
The guy nextdoor

Registered: 09/12/03
Posts: 106
Re: Do colinizing jars need fresh air? [Re: Magash]
    #1982863 - 10/06/03 12:03 AM (20 years, 5 months ago)

I'm also curious about re-inoculation. My friend has jar of karo water that has 0 signs of growth after two weeks. I told him not to re-inoculate cuz something could be wrong with the jar itself, but generally speaking is re-inoculation asking for trouble? Also, my friend is having success with a heating pad on low. He has a towel on top of the pad and his jars are on a short rack about 3/4" above the surface for air exchange. (he recently removed tape) Anyway his thermometer reads 80 resting right on top of the towel.



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Doesn't have the patience to grow shrooms himself, but instead mooches off friend in exchange for research. :tongue:

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OfflineCubieman420
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Registered: 03/20/02
Posts: 2,693
Loc: Midwest
Last seen: 10 years, 3 months
Re: Do colinizing jars need fresh air? [Re: Hertyar]
    #1982968 - 10/06/03 01:21 AM (20 years, 5 months ago)

One would assume that as long as the jars are still sterile that re-innoculating would work, but who knows.....anyone done this??


--------------------
"...now waters run free, no more fish in the sea..."
1983-2004

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InvisibleHanky
wiffle bat.
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Registered: 08/30/03
Posts: 56,993
Loc: Great Southern Land.
Re: Do colinizing jars need fresh air? [Re: Cubieman420]
    #1983262 - 10/06/03 06:29 AM (20 years, 5 months ago)

heater pads are fucked..one of those damn contraptions cooked 3 of my first 13 jars....build a magash incubator and you'll never look back.


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Coaster is an idiot...
[quote]Coaster said:
but i thnk everything thats pure is white?
[/quote]



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