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Invisibleelasticaltiger
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Registered: 06/24/13
Posts: 8,438
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Re: MS to LC, the worst way i know.. but why exactly? [Re: Saint]
    #19771616 - 03/31/14 02:24 AM (10 years, 9 months ago)

Quote:

Mr. Alien said:
:goodburger: now i understand.



Quote:

cronicr said:
:ohokayigetit:



Quote:

Saint said:
Right thats is my point too. IF you have more contam's then real spore your going to lose. and remember the higher the temp the faster cottam's grow. best to transfer your dish to a new dish as soon as you see growth and keep going till you have the best dish posable. maybe a month or 2 but then make a LC from a clean agar dish and your going to be over run with a clean  grow.. Spore's are very ruff to do  if you unsure about where they came from...Never heat your dish's

Was going to say I use potato starch and agar to make my dishes.  at times i use the starch to make my LC too you can do many things with just starch.




I'm so drunk you don't even know... are you guys being sarcastic or did I really make sense?


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20

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OfflineSaint
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Re: MS to LC, the worst way i know.. but why exactly? [Re: elasticaltiger]
    #19771651 - 03/31/14 02:47 AM (10 years, 9 months ago)

Quote:


I'm so drunk you don't even know... are you guys being sarcastic or did I really make sense?




No worries your great  you hit it on the nail. i  was just adding my 2 cents to what you had said.
If you like to gamble then dont worry what in your MS and just waist 100 jars and hope you get 50 of them to work. but If you know every MS  has a 50/50 rate then you do agar.

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Invisibleelasticaltiger
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Re: MS to LC, the worst way i know.. but why exactly? [Re: Saint]
    #19771664 - 03/31/14 02:55 AM (10 years, 9 months ago)

Quote:

Saint said:
Quote:


I'm so drunk you don't even know... are you guys being sarcastic or did I really make sense?




No worries your great  you hit it on the nail. i  was just adding my 2 cents to what you had said.
If you like to gamble then dont worry what in your MS and just waist 100 jars and hope you get 50 of them to work. but If you know every MS  has a 50/50 rate then you do agar.




oh kay

:drunkdriver:


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20

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InvisibleMr. Alien
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Posts: 6,290
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Re: MS to LC, the worst way i know.. but why exactly? [Re: elasticaltiger]
    #19772301 - 03/31/14 09:08 AM (10 years, 9 months ago)

Quote:

elasticaltiger said:
Quote:

Mr. Alien said:
:goodburger: now i understand.



Quote:

cronicr said:
:ohokayigetit:



Quote:

Saint said:
Right thats is my point too. IF you have more contam's then real spore your going to lose. and remember the higher the temp the faster cottam's grow. best to transfer your dish to a new dish as soon as you see growth and keep going till you have the best dish posable. maybe a month or 2 but then make a LC from a clean agar dish and your going to be over run with a clean  grow.. Spore's are very ruff to do  if you unsure about where they came from...Never heat your dish's

Was going to say I use potato starch and agar to make my dishes.  at times i use the starch to make my LC too you can do many things with just starch.




I'm so drunk you don't even know... are you guys being sarcastic or did I really make sense?




I'm serious! i mean, you are the only one that made an explanation.. and i believe you.

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Invisibleazur
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Re: MS to LC, the worst way i know.. but why exactly? [Re: Mr. Alien]
    #19772324 - 03/31/14 09:15 AM (10 years, 9 months ago)

I've gone ms to lc many times. If you know what healthy mycelium looks like in lc, you CAN spot a bad one. And I'd say you've got a 75 percent chance of it working out.


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!


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InvisibleMr. Alien
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Re: MS to LC, the worst way i know.. but why exactly? [Re: azur]
    #19772442 - 03/31/14 09:56 AM (10 years, 9 months ago)

you should post pics, of healthy MYC of a MSLC, and not healthy MYC of a MSLC.

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Invisibleazur
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Re: MS to LC, the worst way i know.. but why exactly? [Re: Mr. Alien]
    #19772521 - 03/31/14 10:17 AM (10 years, 9 months ago)

Will do


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!


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OnlinecronicrMFacebook
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Re: MS to LC, the worst way i know.. but why exactly? [Re: Mr. Alien]
    #19772529 - 03/31/14 10:21 AM (10 years, 9 months ago)

Quote:

azur said:
I've gone ms to lc many times. If you know what healthy mycelium looks like in lc, you CAN spot a bad one. And I'd say you've got a 75 percent chance of it working out.



:facepalm3: you cant always spot a bad one
Quote:

Mr. Alien said:
you should post pics, of healthy MYC of a MSLC, and not healthy MYC of a MSLC.



that wont do you any good, the only way to know is a test jar not a pic


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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OfflineRogerRabbitM
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Re: MS to LC, the worst way i know.. but why exactly? [Re: azur]
    #19772537 - 03/31/14 10:23 AM (10 years, 9 months ago)

Quote:

MS to LC, the worst way i know.. but why exactly?




Two main reasons;

It's slower than growing directly from spores.

It's an unnecessary additional vector of contamination.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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InvisibleMr. Alien
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Re: MS to LC, the worst way i know.. but why exactly? [Re: cronicr]
    #19772575 - 03/31/14 10:33 AM (10 years, 9 months ago)

Quote:

cronicr said:
Quote:

azur said:
I've gone ms to lc many times. If you know what healthy mycelium looks like in lc, you CAN spot a bad one. And I'd say you've got a 75 percent chance of it working out.



:facepalm3: you cant always spot a bad one
Quote:

Mr. Alien said:
you should post pics, of healthy MYC of a MSLC, and not healthy MYC of a MSLC.



that wont do you any good, the only way to know is a test jar not a pic




i know... that's why i said that.. is impossible to know by naked eye.

:guiltyascharged:

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OnlinecronicrMFacebook
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Re: MS to LC, the worst way i know.. but why exactly? [Re: Mr. Alien]
    #19772584 - 03/31/14 10:35 AM (10 years, 9 months ago)

:lol:


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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InvisibleMr. Alien
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Re: MS to LC, the worst way i know.. but why exactly? [Re: RogerRabbit]
    #19772619 - 03/31/14 10:43 AM (10 years, 9 months ago)

Indeed RR.. :bow2:

Somebody confirms if what tiger deduced is correct?

Who cares...MSLC is a crap.. lol

Edited by Mr. Alien (03/31/14 10:54 AM)

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Invisibleblindingleaf
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Re: MS to LC, the worst way i know.. but why exactly? [Re: Mr. Alien]
    #19773382 - 03/31/14 02:16 PM (10 years, 9 months ago)

yes what tiger said is correct.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

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InvisibleMr. Alien
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Re: MS to LC, the worst way i know.. but why exactly? [Re: blindingleaf]
    #19773618 - 03/31/14 03:23 PM (10 years, 9 months ago)

:bow2: Tiger :bow2:

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Re: MS to LC, the worst way i know.. but why exactly? [Re: Mr. Alien]
    #19775135 - 03/31/14 09:40 PM (10 years, 9 months ago)

I have to ask  just for the  hell of it. all the venders sell these MS to people with contams in them and  i know a spore print would be a lot better to  use. Just all someone has is a MS  to use. Now we know its a crap shot in most cases. What is the best way to go about using these syringes.  Do we still want  to do a drop into agar? wait till there is  some white myclem  growth and transfer it  to a new dish? Or shot up some grain and take  a spot  to a agar dish and  try to transfer to a clean  growth get rid of the water is  the  hard part I wish I was  smart  and just found a vender  with prints in the first place.  But can some one say  the best procedure to make a syringe work out.  With out just a roll of them dice.

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Invisibleelasticaltiger
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Re: MS to LC, the worst way i know.. but why exactly? [Re: Saint]
    #19775363 - 03/31/14 10:26 PM (10 years, 9 months ago)

Quote:

Saint said:
I have to ask  just for the  hell of it. all the venders sell these MS to people with contams in them and  i know a spore print would be a lot better to  use. Just all someone has is a MS  to use. Now we know its a crap shot in most cases. What is the best way to go about using these syringes.  Do we still want  to do a drop into agar? wait till there is  some white myclem  growth and transfer it  to a new dish? Or shot up some grain and take  a spot  to a agar dish and  try to transfer to a clean  growth get rid of the water is  the  hard part I wish I was  smart  and just found a vender  with prints in the first place.  But can some one say  the best procedure to make a syringe work out.  With out just a roll of them dice.




You'll probably get a lot of different answers to this.  Some people may tell you to squirt it on to agar.

Personally, I say just do PF Tek to get yourself some fast fruits. Then clone a healthy looking fruit or make your own prints and run agar from that.

A spore syringe from a reputable vendor is probably safe to use for brown rice jars and I've used them for bulk as well. If vendor made spore syringes weren't at least SOMEWHAT reliable no one would ever use them.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20

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OnlinecronicrMFacebook
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Re: MS to LC, the worst way i know.. but why exactly? [Re: elasticaltiger]
    #19775392 - 03/31/14 10:34 PM (10 years, 9 months ago)

:whathesaid:they replace them when there not so knock shit up and hope for the best


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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OfflineSaint
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Re: MS to LC, the worst way i know.. but why exactly? [Re: cronicr]
    #19886507 - 04/23/14 05:16 AM (10 years, 8 months ago)

Ok sorry was  haveing issues  with upload of photos  but  got some good ones  now



ok  this took for ever to get going took one drop of spore's in Syringe. took like 2 weeks to make a pin size and then it exploded Agave  is what i used  when it get done i will agar to make sure but it looks so good for a cube spore

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OfflineSaint
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Re: MS to LC, the worst way i know.. but why exactly? [Re: Saint]
    #19886524 - 04/23/14 05:28 AM (10 years, 8 months ago)

and  now for a wird looking one  made from agar

the size of little pins

as you can see  just nothing to them not fluffy like i thought i should be




Anyone know about why a classic cube  would look this way ?its in 4 different jars used  raw sugar, Agave, corn syrup  lite.  even the chuck  of agar one  looks different. andy ideas would be a help on these cubes .and its not con tam  i know that much

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Invisibleblindingleaf
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Re: MS to LC, the worst way i know.. but why exactly? [Re: Saint]
    #19886562 - 04/23/14 06:01 AM (10 years, 8 months ago)

even with agar, u won't know its a contam till u try it man, sorry to say.  i only make LC with agar, and it still gets fucked up occasionally.  u really really can't tell if its con tamed if its LC, i promise.  certain things u will know for sure (prolonged bubbles after shaking is bad, black, green, etc is bad), but overall its very difficult to tell if the LC is clean.
those aren't pins either, its just mycelium suspended in water.  ur pics are blurry, not that it matter b.c its LC, but with an agar drop into LC, the wedge will start to look fluffy as it grows out (kinda like when u drop in grain, and it gets fluffy around the agar before it spreads to the grain if u left the wedge on top) at which point, swirl it up, and those marbles will help get the myc off the wedge and starting their own growth.  DEFINITELY test it on a another plate before using.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

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