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InvisibleThe Lightning
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Re: Guess That Fungi/Mushroom Chemical!! [Re: MachineElf1.618]
    #19608947 - 02/23/14 03:28 PM (10 years, 1 day ago)

Here is a different version of the same chemical, listed by a different author:



Hint: The genus that this fungi is in is Hypocrea.


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InvisibleMr. Bojangles
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Re: Guess That Fungi/Mushroom Chemical!! [Re: The Lightning]
    #19615824 - 02/25/14 09:42 AM (10 years, 5 hours ago)

Was trying to give other people a shot but it looks like this thread is slowing down a bit...

Fungus is Hypocrea lixii and the chemical is cajanol.  The fungus grows on it's host: the "pigeon pea" plant (Cajanus cajun for which the chemical is named).  Cajanol induces apoptosis after stopping the cell cycle between the G2 and the M phase.


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"It is dangerous to be right in matters on which the established authorities are wrong."

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InvisibleThe Lightning
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Re: Guess That Fungi/Mushroom Chemical!! [Re: Mr. Bojangles]
    #19618472 - 02/25/14 08:29 PM (9 years, 11 months ago)

Well done, Mr. Bojangles.

Hit us with something exciting, intense, or frightening.


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Offlinespore baby
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Re: Guess The Fungi/Mushroom Chemical ! [Re: The Lightning]
    #19618525 - 02/25/14 08:39 PM (9 years, 11 months ago)

.


Edited by spore baby (12/19/14 09:02 PM)


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InvisibleMr. Bojangles
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Re: Guess The Fungi/Mushroom Chemical ! [Re: spore baby]
    #19618692 - 02/25/14 09:14 PM (9 years, 11 months ago)

Alright I'm going with a protein here, good luck guys.  This is an enzyme that isn't just found in the fungus that I posted but all of eukaryota.  Notice the particular 'C' shape of the protein, which allows it to perform a certain function such as something fits into that C groove :wink:.  Coincidentally this enzyme helps to catalyze the formation of something that can be used to create more of the subunits that make up this complex...trippy :cool:





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OfflineMachineElf1.618
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Re: Guess The Fungi/Mushroom Chemical ! [Re: Mr. Bojangles]
    #19619401 - 02/26/14 02:21 AM (9 years, 11 months ago)

The enzyme is RNA polymerase. You can't really see this picture well but the purplish atom in the middle there is the Mg2+ ion that facilitates the catalytic activity. The molecule in red there is the ribonucleotide that gets added to the elongating mRNA chain during gene transcription.

I was thinking the mushroom is probably Amanita vernicoccora because of the patch but I don't really see a striate margin and the color also looks more like A. phalloides. So I'm gonna go with A. phalloides, I'm thinking its fitting since the amatoxins in the death cap are potent inhibitors of RNA polymerase.


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InvisibleMr. Bojangles
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Re: Guess The Fungi/Mushroom Chemical ! [Re: MachineElf1.618]
    #19619782 - 02/26/14 07:30 AM (9 years, 11 months ago)

Correct!  More specifically it is RNA Polymerase II.  The red in the catalytic site of the protein is actually alpha-amanitin (the amatoxin found in A. phalloides) so the picture is essentially showing how the death cap kills you :grin:

Alright, you're up!


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OfflineMachineElf1.618
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Re: Guess That Fungi/Mushroom Chemical!! [Re: Mr. Bojangles]
    #19623669 - 02/26/14 09:46 PM (9 years, 11 months ago)


This is RNA with associated protein and is a subunit of the bigger molecule. I think should be a big enough hint.



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InvisibleMr. Bojangles
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Re: Guess That Fungi/Mushroom Chemical!! [Re: MachineElf1.618]
    #19625223 - 02/27/14 09:21 AM (9 years, 11 months ago)

40S subunit of the ribosome complex and Pleurotus pulmonarius?


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InvisibleRuralAnomaly
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Re: Guess That Fungi/Mushroom Chemical!! [Re: MachineElf1.618]
    #19626452 - 02/27/14 02:13 PM (9 years, 11 months ago)

Quote:

mtlmike90 said:
I think should be a big enough hint.






lol

i'd need a much bigger hint, more like a paragraph, maybe even a tome.

:popcorn:


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OfflineMachineElf1.618
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Re: Guess That Fungi/Mushroom Chemical!! [Re: Mr. Bojangles]
    #19626672 - 02/27/14 03:04 PM (9 years, 11 months ago)

Quote:

Mr. Bojangles said:
40S subunit of the ribosome complex and Pleurotus pulmonarius?




Yes it is the 40S small subunit. The species is not P. pulmonarius but the genus is correct. The oysters in the pic don't have a stipe or at least its not as pronounced as it would be in P. pulmonarius.


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InvisibleMr. Bojangles
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Re: Guess That Fungi/Mushroom Chemical!! [Re: MachineElf1.618]
    #19626836 - 02/27/14 03:42 PM (9 years, 11 months ago)

Second guess would be P. ostreatus in that case.  It just looked a bit more pale than the ones I'm used to cultivating.


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OfflineMachineElf1.618
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Re: Guess That Fungi/Mushroom Chemical!! [Re: Mr. Bojangles]
    #19626899 - 02/27/14 03:56 PM (9 years, 11 months ago)

Yup its P. ostreatus. I don't like the color either but thats how the oysters in my area look. I have yet to find one with a darker gray shade. Anyway ur up dude.


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InvisibleMr. Bojangles
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Re: Guess That Fungi/Mushroom Chemical!! [Re: MachineElf1.618]
    #19627015 - 02/27/14 04:24 PM (9 years, 11 months ago)

This protein should be easier to guess for those who know less about biochemistry. 

It is a ubiquitous transmembrane protein found in all life forms but I'm hazarding a guess that it's especially important for fruiting mushies :wink:.  The crystal structure shown is a top-down view of the protein in its 'open' state.  When open, only one specific molecule may flow through the channel in the middle of the protein...the protein is thus named after this molecule. 





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InvisibleRuralAnomaly
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Re: Guess That Fungi/Mushroom Chemical!! [Re: Mr. Bojangles]
    #19627437 - 02/27/14 05:55 PM (9 years, 11 months ago)

well, i've had some fun on wiki, so with that as a basis for my 'education' and a hopeful interpretation of the hint, i'm gonna guess an "aquaporin"

and... the mushroom is Omphalotus illudens?


Edited by RuralAnomaly (02/27/14 06:02 PM)


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InvisibleMr. Bojangles
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Re: Guess That Fungi/Mushroom Chemical!! [Re: RuralAnomaly]
    #19627454 - 02/27/14 05:57 PM (9 years, 11 months ago)

Yep!

:firstladyofapproval:

Care to guess the fungi?


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"It is dangerous to be right in matters on which the established authorities are wrong."

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InvisibleRuralAnomaly
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Re: Guess That Fungi/Mushroom Chemical!! [Re: Mr. Bojangles]
    #19627481 - 02/27/14 06:03 PM (9 years, 11 months ago)

i did care!  belatedly realized and edited... my guess was jack o lanterns


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InvisibleMr. Bojangles
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Re: Guess That Fungi/Mushroom Chemical!! [Re: Mr. Bojangles]
    #19627519 - 02/27/14 06:12 PM (9 years, 11 months ago)

Oh, I see it now...got em both right...you're up!


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InvisibleRuralAnomaly
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Re: Guess That Fungi/Mushroom Chemical!! [Re: Mr. Bojangles]
    #19627571 - 02/27/14 06:29 PM (9 years, 11 months ago)

this molecule forms the basis of a class of antibiotics that *drum roll*
inhibit protein synthesis in bacteria by binding to the 50S subunit of ribosomes. (totally searchable phrase if you really want to *hem* shortcut to know)
- and something i understand a large fraction better now after reading those pages about ribosomal subunits! whoo hooo.  isn't this fun!?



this compound was first isolated from a Clitopilus sp.

and i'm not being sarcastic about the fun part


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Re: Guess That Fungi/Mushroom Chemical!! [Re: Mr. Bojangles]
    #19628241 - 02/27/14 09:30 PM (9 years, 11 months ago)

WHAT MY ANSWER DIDN't POST!!!!!!
Oh well, good job rural anomaly.

Mr. Bojangles I know what you were thinking but I don't think aquaporins are what help fungi grow. In animals aquaporins work to keep osmotic pressure and a chemical gradiant as well as allow water to flow between cells in tissues like the kidney. I know in plants aquaporins help with water transport from the roots to the the rest of the plant. In fungi though I'm not sure. especially because hyphal cells are seperated by septum which already is a big passage for water.


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