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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: bodhisatta]
#19618808 - 02/25/14 09:42 PM (9 years, 11 months ago) |
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indeed!
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: blindingleaf]
#19618885 - 02/25/14 10:03 PM (9 years, 11 months ago) |
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I love your petri pictures, all that looks great to me
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1down5up
Social Ninja



Registered: 11/04/13
Posts: 1,743
Last seen: 1 year, 3 months
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: bodhisatta]
#19622526 - 02/26/14 05:53 PM (9 years, 11 months ago) |
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Here's a mini mono i fruited using one of the agar wedges from a second transfer. They were plates done just prior to the plates i sent out...so would have been second transfer. Cloned tissue, second transfer. I was after cutting a few, didn't want any caps splooogin' all over the place.

Thanks again for the reishi bro, as soon as i free up a FC, i'll be diving into the Reishi, head first, similar to Pastywhite's method. I'll keep ya up on it's progress
-------------------- ~~Everything is relative~~ A Simplified Overview of Mushroom Cultivation Strategies - RR says - EvilMushroom666's Take on BRF Cakes - Frank's list of goodies - Cronicr's Goodies No one is placed higher than another no matter race or creed or gender, we must teach forgiveness and compassion for all life. J.L.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: 1down5up]
#19622531 - 02/26/14 05:54 PM (9 years, 11 months ago) |
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SORRY BOYS! been slacking on this one just got too much happening atm, my shits ready to rock though
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: 1down5up]
#19622532 - 02/26/14 05:54 PM (9 years, 11 months ago) |
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Quote:
1down5up said:

Looks like your conditions are good after all, but the genetics are not so much.
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1down5up
Social Ninja



Registered: 11/04/13
Posts: 1,743
Last seen: 1 year, 3 months
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: FrankHorrigan]
#19622590 - 02/26/14 06:05 PM (9 years, 11 months ago) |
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When you said my conditions were poor, i didn't know where to start...pasty told me it looked a little dry, so i ditched the fan. Turns out, i don't need it, air exchanger provides enough current for FAE.
Genetics are meh. I cloned a piece of my first fruit. I have a plate with one transfer after this one, which would be the third. I'm happy with the size of the fruits, but not the pinset. Oh well, chalk this one up to exp.
-------------------- ~~Everything is relative~~ A Simplified Overview of Mushroom Cultivation Strategies - RR says - EvilMushroom666's Take on BRF Cakes - Frank's list of goodies - Cronicr's Goodies No one is placed higher than another no matter race or creed or gender, we must teach forgiveness and compassion for all life. J.L.
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: 1down5up]
#19622704 - 02/26/14 06:28 PM (9 years, 11 months ago) |
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Hey frank or Cron what do you think of my plates

I'm thinking they went tomentose from using the same agar media for all 7 transfers so far, but other than that I think the bottom right and bottom middle are isolates
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: bodhisatta]
#19623148 - 02/26/14 08:00 PM (9 years, 11 months ago) |
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Quote:
'm thinking they went tomentose from using the same agar media for all 7 transfers so far, but other than that I think the bottom right and bottom middle are isolates
i got worried about this too at times. except i read that it does not go tomentose as much as the culture basically only learns to "eat one thing" instead of having a varied diet, so when u put it to grain, it can be slow. then i read an RR quote where he kinda disputed it, saying he just uses MEA all the time, so i dunno what to think. either way, i started doing standard mea then grain water MEA and alternate back and forth b/c all i have is pre made mea powder, so i try to vary the stuff i put in the added water.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Sgt. Pepper



Registered: 06/19/13
Posts: 2,538
Loc: Third Stone From The Sun
Last seen: 2 months, 17 days
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: blindingleaf]
#19623187 - 02/26/14 08:06 PM (9 years, 11 months ago) |
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Quote:
i started doing standard mea then grain water MEA and alternate back and forth b/c all i have is pre made mea powder, so i try to vary the stuff i put in the added water.
Great idea! I've only got premade mea and I was starting to worry about this too, so I'm really glad you said something!
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: Sgt. Pepper]
#19623207 - 02/26/14 08:11 PM (9 years, 11 months ago) |
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i do 250 rye soak water, 250 tap. sometimes there is coffee in the rye water too, other times i just boil 1/2 cup rye berries for two hours till a lot get burst, strain and add that. maybe it makes it too nutritious to be optimum, and that may slow it down i have read (more food to digest so growth is slowed?), but my petris are sitting at 81-82 usually above all my jars, so they grow quick.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: blindingleaf]
#19623229 - 02/26/14 08:15 PM (9 years, 11 months ago) |
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I wish they made rye malt extract in the spray malt but I can only find the liquid that would be a great agar for us rye grain people to use. extra light rye malt extract
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: bodhisatta]
#19623254 - 02/26/14 08:19 PM (9 years, 11 months ago) |
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i found an awesome Rye beer the other day i wanted to try that with plain agar for exact reason u said bodhi. u think the hops would mess it up? i know nothing of beer like its ph and all that, so i wasn't sure if that could work.
it was this beer
edit: super tasty as well
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
Edited by blindingleaf (02/26/14 08:20 PM)
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Sgt. Pepper



Registered: 06/19/13
Posts: 2,538
Loc: Third Stone From The Sun
Last seen: 2 months, 17 days
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: blindingleaf]
#19623263 - 02/26/14 08:21 PM (9 years, 11 months ago) |
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Quote:
blindingleaf said: i found an awesome Rye beer the other day i wanted to try that with plain agar for exact reason u said bodhi. u think the hops would mess it up? i know nothing of beer like its ph and all that, so i wasn't sure if that could work.
it was this beer
That might be interesting. I'd boil off all the alcohol first though, I doubt mycelium would perform well drunk. Haha.
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: Sgt. Pepper]
#19623283 - 02/26/14 08:26 PM (9 years, 11 months ago) |
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word, i was going to just leave it out overnight, but boiling does the same thing faster
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: blindingleaf]
#19623294 - 02/26/14 08:28 PM (9 years, 11 months ago) |
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Quote:
blindingleaf said: i found an awesome Rye beer the other day i wanted to try that with plain agar for exact reason u said bodhi. u think the hops would mess it up? i know nothing of beer like its ph and all that, so i wasn't sure if that could work.
it was this beer
edit: super tasty as well
I wouldn't think the hops would hurt, Yeast grows just fine with loads of hops, Hops only seem to fuck with bacteria, but it might be a different story for this part of the fungi kingdom.
looks delicious
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Sgt. Pepper



Registered: 06/19/13
Posts: 2,538
Loc: Third Stone From The Sun
Last seen: 2 months, 17 days
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: bodhisatta]
#19623444 - 02/26/14 08:57 PM (9 years, 11 months ago) |
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That would be cool if it turned out hops made kick ass antibacterial agar! Yeah, building would be quick, you actually would just have to keep the temperature over 170 for a while to boil the alcohol.
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: Sgt. Pepper]
#19623494 - 02/26/14 09:09 PM (9 years, 11 months ago) |
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Quote:
looks delicious
indeed! 1st thing i would do if anyone from here visited me is go to that brewery, get drunk, come back get stoned, take weed nap then talk cultivation 
Quote:
That would be cool if it turned out hops made kick ass antibacterial agar!
that would be awesome! "how to dial in ur petris like a boss" lol
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: blindingleaf]
#19623511 - 02/26/14 09:12 PM (9 years, 11 months ago) |
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bodhi u probably drink awesome beer a lot tho, right? if u have any recommendations, lemme know, ill keep an eye out. I'm no beer snob at all, well maybe on pay day, but i know nothing about this hops or that malt or conditioning, etc. i just taste, enjoy, and occasionally read beer advocate's ratings
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: bodhisatta]
#19623519 - 02/26/14 09:14 PM (9 years, 11 months ago) |
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Quote:
bodhisatta said:

Hey frank or Cron what do you think of my plates

I'm thinking they went tomentose from using the same agar media for all 7 transfers so far, but other than that I think the bottom right and bottom middle are isolates
give a quick switch up and check it out
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: bodhisatta]
#19623591 - 02/26/14 09:30 PM (9 years, 11 months ago) |
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Out of all the talk in this thread, I cannot believe how much is about agar stuff...don't worry so much about the myc on the plate, if they are clean then test those bad boys out!
There is only one way to tell if it's going to be a good fruiter and that is by fruiting it.
Also, Bodhi, your plates look great. They look like isolates or damn close
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