Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   PhytoExtractum Kratom Powder for Sale   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Bags   Myyco.com Isolated Cubensis Liquid Culture For Sale   Kraken Kratom Red Vein Kratom   Original Sensible Seeds Bulk Cannabis Seeds   OlympusMyco.com Olympus Myco Bulk Substrate

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineSynapseLotus


Registered: 01/18/14
Posts: 130
Loc: Δx = ħ/(2•Δp)
Last seen: 8 years, 11 months
Fruit to agar, why sample base of stem?
    #19598162 - 02/21/14 05:00 AM (10 years, 4 months ago)

Hi all,

I just read that you should sample from the base of the stem when dropping into agar. I happen to have just sampled a shiitake from right where the cap and stem meets just five minutes ago.

I couldn't find any info on why it is good to sample from the base. Can anyone help me out, and also tell me if I am OK with my current sample, or what the disadvantage are to what I did?

Cheers,


--------------------
See my shadow changing, Stretching up and over me.
Soften this old armor, hoping I can clear the way.
By stepping through my shadow, coming out the other side.
Step into the shadow, 46 and 2 are just ahead of me.

Spongiform's Plastic Tek
My gift to the Shroomery
Stan Getz/Bill Evans Masterpiece
The most important video of this generation
NO CUT, PERFECT MONOTUB HOLES!

Edited by SynapseLotus (02/21/14 05:10 AM)

Extras: Filter Print Post Top
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 4 months
Trusted Cultivator
OG Cultivator
Re: Fruit to agar, why sample base of stem? [Re: SynapseLotus]
    #19598169 - 02/21/14 05:05 AM (10 years, 4 months ago)

That's probably because someone did it that way and thought that was the only way.  I usually get my clones from the middle of the stem or near the stem/cap interface.  If you're cloning a small pin, just drop the whole thing on agar.  The mycelium will grow faster than the contaminant spores can germinate, so after 1 cm or less of growth, transfer from the leading edge to new dishes.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

Extras: Filter Print Post Top
OfflineSynapseLotus


Registered: 01/18/14
Posts: 130
Loc: Δx = ħ/(2•Δp)
Last seen: 8 years, 11 months
Re: Fruit to agar, why sample base of stem? [Re: RogerRabbit]
    #19598178 - 02/21/14 05:14 AM (10 years, 4 months ago)

So I guess the way I did it is fine after all.

I also dropped a cube pin (barely 1cm long) into a dish 2 days ago and I only saw a tiny bit of fuzz on top. I then went back in, split it open (damn those new pins are hard as rocks!) and then pushed it down so that it split in half and spread the middle across the agar. I'm hoping for better results that way.

Thanks for the help RR.


--------------------
See my shadow changing, Stretching up and over me.
Soften this old armor, hoping I can clear the way.
By stepping through my shadow, coming out the other side.
Step into the shadow, 46 and 2 are just ahead of me.

Spongiform's Plastic Tek
My gift to the Shroomery
Stan Getz/Bill Evans Masterpiece
The most important video of this generation
NO CUT, PERFECT MONOTUB HOLES!

Edited by SynapseLotus (02/21/14 05:16 AM)

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore North Spore Mushroom Grow Kits & Cultivation Supplies   PhytoExtractum Kratom Powder for Sale   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Bags   Myyco.com Isolated Cubensis Liquid Culture For Sale   Kraken Kratom Red Vein Kratom   Original Sensible Seeds Bulk Cannabis Seeds   OlympusMyco.com Olympus Myco Bulk Substrate


Similar ThreadsPosterViewsRepliesLast post
* why are my stems so narrow? (small shrooms) KerPluNk 946 5 05/12/02 01:58 PM
by windex
* Re: i this a picture of psilocybe mexicana fruiting in a petri dish? mycofile 4,864 10 02/05/00 11:45 AM
by Mycelium5150
* Help with fruiting-- something went wrong Philip J. Fry 247 6 03/04/18 10:39 PM
by Philip J. Fry
* why clones to Agar? Why not just clones to bseed? BillyGrass 1,409 3 04/15/02 04:57 PM
by Alkaloids
* Favourite tek to make syringe from live fruit?? Dogomush 905 6 11/19/02 07:22 PM
by dankman68
* Smallest fruit?
( 1 2 all )
SixCee 4,609 38 12/30/02 12:55 PM
by SixCee
* How do you use agar? Sporpheus 9,085 5 05/11/01 08:46 PM
by egghead
* Mycelium on stems/caps MushroomMatt 2,886 5 10/16/01 11:16 AM
by Insomnia

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
457 topic views. 16 members, 75 guests and 148 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.018 seconds spending 0.005 seconds on 12 queries.