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SynapseLotus


Registered: 01/18/14
Posts: 130
Loc: Δx = ħ/(2•Δp)
Last seen: 8 years, 11 months
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Fruit to agar, why sample base of stem?
#19598162 - 02/21/14 05:00 AM (10 years, 4 months ago) |
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Hi all,
I just read that you should sample from the base of the stem when dropping into agar. I happen to have just sampled a shiitake from right where the cap and stem meets just five minutes ago.
I couldn't find any info on why it is good to sample from the base. Can anyone help me out, and also tell me if I am OK with my current sample, or what the disadvantage are to what I did?
Cheers,
-------------------- See my shadow changing, Stretching up and over me. Soften this old armor, hoping I can clear the way. By stepping through my shadow, coming out the other side. Step into the shadow, 46 and 2 are just ahead of me. Spongiform's Plastic Tek My gift to the Shroomery Stan Getz/Bill Evans Masterpiece The most important video of this generation NO CUT, PERFECT MONOTUB HOLES!
Edited by SynapseLotus (02/21/14 05:10 AM)
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 4 months
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Re: Fruit to agar, why sample base of stem? [Re: SynapseLotus]
#19598169 - 02/21/14 05:05 AM (10 years, 4 months ago) |
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That's probably because someone did it that way and thought that was the only way. I usually get my clones from the middle of the stem or near the stem/cap interface. If you're cloning a small pin, just drop the whole thing on agar. The mycelium will grow faster than the contaminant spores can germinate, so after 1 cm or less of growth, transfer from the leading edge to new dishes. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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SynapseLotus


Registered: 01/18/14
Posts: 130
Loc: Δx = ħ/(2•Δp)
Last seen: 8 years, 11 months
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Re: Fruit to agar, why sample base of stem? [Re: RogerRabbit]
#19598178 - 02/21/14 05:14 AM (10 years, 4 months ago) |
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So I guess the way I did it is fine after all.
I also dropped a cube pin (barely 1cm long) into a dish 2 days ago and I only saw a tiny bit of fuzz on top. I then went back in, split it open (damn those new pins are hard as rocks!) and then pushed it down so that it split in half and spread the middle across the agar. I'm hoping for better results that way.
Thanks for the help RR.
-------------------- See my shadow changing, Stretching up and over me. Soften this old armor, hoping I can clear the way. By stepping through my shadow, coming out the other side. Step into the shadow, 46 and 2 are just ahead of me. Spongiform's Plastic Tek My gift to the Shroomery Stan Getz/Bill Evans Masterpiece The most important video of this generation NO CUT, PERFECT MONOTUB HOLES!
Edited by SynapseLotus (02/21/14 05:16 AM)
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