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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: J. Jack Flash]
#19576524 - 02/16/14 06:56 PM (10 years, 8 days ago) |
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rock on dude! looks like u got a clean lc!
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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blueconfusion
Strangest


Registered: 12/14/12
Posts: 1,727
Last seen: 1 day, 6 hours
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: blindingleaf]
#19584232 - 02/18/14 08:09 AM (10 years, 7 days ago) |
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i transferred some agar plates to grains last week and just did the 30% shake the other day. so since i won't have the opportunity to prep grains until idk when i started last night while i have some time off work. thought i had enough wbs to do all my jars, but i did not so instead of running to the store i mixed wbs and some rye berries about a 3:1 ratio put some coffee and gypsum in and am putting them in the pc right now. it's either gonna be the crs, oak hills or creepers whichever is 100% first, but those are my fastest jars so far.
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: blueconfusion]
#19590892 - 02/19/14 05:20 PM (10 years, 5 days ago) |
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Nothing to really update with the creeper, all the growth is going so slow on everything in my house.
I may have got some of the pan FL print to germinate some new dishes the Pan RDU is no luck so far and the Pan tropic seems to have germinated cleanly on agar and is going very slow in that grain jar. The blue oysters I got are taking off on there new dishes without problem. Pics in a few days.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: bodhisatta]
#19590950 - 02/19/14 05:34 PM (10 years, 5 days ago) |
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my jars are done, waiting on coir now my creeper was awesomely clean and cheers to that bod
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: cronicr]
#19590978 - 02/19/14 05:38 PM (10 years, 5 days ago) |
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I think im on the 7th and 8th transfers now.
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Gorlami



Registered: 08/28/13
Posts: 328
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: bodhisatta]
#19592496 - 02/19/14 10:50 PM (10 years, 5 days ago) |
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Quote:
bodhisatta said: I think im on the 7th and 8th transfers now.
Is there any way to guestimate how many more transfers will be needed?
As for me, I'm on 6th for my Texas plate, I can start seeing some sectoring... and it's a thing of beauty!
I inoculated some WBS and Rye grain with my plates from 4th transfer, WBS seems to be much faster and I should have spawn ready for a monotub very soon. Bought some 66qt totes, can't wait!
Wish I could post some pictures, but no pour containers aren't great for showing mycelium. 
Will update when I have something to show you all.
Later hombres!
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PirateSwazey



Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: 1down5up]
#19592769 - 02/20/14 12:03 AM (10 years, 5 days ago) |
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Quote:
Pastywhyte said:

Pasty that shit is boss! 
Are you keeping a grow log of your reishi somewhere bud?
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CMOS
Whats next?


Registered: 01/08/12
Posts: 833
Last seen: 9 years, 9 months
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: PirateSwazey]
#19592815 - 02/20/14 12:19 AM (10 years, 5 days ago) |
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Quote:
PirateSwazey said:
Quote:
Pastywhyte said:

Pasty that shit is boss! 
Are you keeping a grow log of your reishi somewhere bud?
Yeah I'd like tto see that if its around... I gots some reshi cultures sitting around, might need to bust em out.
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Gorlami



Registered: 08/28/13
Posts: 328
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: CMOS]
#19592886 - 02/20/14 12:52 AM (10 years, 5 days ago) |
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: Gorlami]
#19593412 - 02/20/14 05:47 AM (10 years, 5 days ago) |
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Quote:
whalechunks said: Pasty Reishi Grow

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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: Gorlami]
#19593918 - 02/20/14 09:56 AM (10 years, 5 days ago) |
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Quote:
whalechunks said:
Is there any way to guestimate how many more transfers will be needed?
Will update when I have something to show you all.
I think I have one or two isolates now, the rest are probably pretty close. I'm just going for blind isolates, hoping to have 6 of them to test for this thread and all of them might suck ass, but hopefully not.
I'll also update when I have something to show, damn mycelium is going all slow
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: bodhisatta]
#19594065 - 02/20/14 10:36 AM (10 years, 5 days ago) |
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i'll post pics of my creeper and KSSS later. i think i need to make one transfer per plate at this point. when i do 3/plate, the sectors don't have a chance to grow out enough, an di keep transferring more than one sector, even tho it looks like I'm transferring only one with the KSSS. the creeper is not seeming to sector
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: blindingleaf]
#19594899 - 02/20/14 02:03 PM (10 years, 5 days ago) |
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ok so here is creeper on its 6th plate from spore. bodhi, what do u think? i saw sectoring on the 4th plate, transferred leading edge of rhizo, then sectoring disappeared.

here are 2 plates, both KSSS for this thread's grow along. this is the 8th plate. i will transfer the obvious choices later, each wedge to its own plate to get better idea of middle of sector.


ps bodhi, my centrifuge tubes are being sterilized right now, i got ur plate! thx again man. i will include a couple surprises to make up for my late send back time.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: blindingleaf]
#19594923 - 02/20/14 02:08 PM (10 years, 5 days ago) |
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beats me, I'm not experienced enough with mycelium on agar morphology, That's why I'm just doing transfers like mad until I get something that looks like the isolates I have seen, maybe it's futile but it's what I felt like doing.
also
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: bodhisatta]
#19594939 - 02/20/14 02:10 PM (10 years, 5 days ago) |
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Quote:
beats me, I'm not experienced enough with mycelium on agar morphology, That's why I'm just doing transfers like mad until I get something that looks like the isolates I have seen, maybe it's futile but it's what I felt like doing.
lol me too man, me too
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Sgt. Pepper



Registered: 06/19/13
Posts: 2,538
Loc: Third Stone From The Sun
Last seen: 2 months, 17 days
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: blindingleaf]
#19594950 - 02/20/14 02:12 PM (10 years, 5 days ago) |
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Same, except for the part where I remember to make a transfer haha.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: bodhisatta]
#19595045 - 02/20/14 02:30 PM (10 years, 5 days ago) |
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Quote:
bodhisatta said:
 2/10/14
 2/14/14
here's the dishes grown out some more. I put them in the same order for the update picture, Looks like bottom middle is an isolate
so here's the last update
 here's those dishes on 2/20/14
and here's the transfers I made from those dishes back on the 14th so they've been growing out 6 days

 Two different views of the same picture
Here's the edible stuff and one perhaps good dish of pan FL



Frank!!! what do you think of my creeper dishes, I'm worried about the way they look. do they look like isolates? they're on the 6th and 7th transfers, not 8th like I had thought.
Edited by Trusted cuItivator (02/20/14 02:33 PM)
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: bodhisatta]
#19595742 - 02/20/14 04:48 PM (10 years, 4 days ago) |
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i gotta starting doing one wedge to a plate after the 4-5th transfer i can see sectors so much easier. on ur 2/20 plates i think that first plate (top left) may be an iso, that last plate (bottom right) has 6 sectors? maybe transfer from 11-12 or 12-3? but like i said I'm so bad at distinguishing isolates, so i dunno 
i was wondering when i would see that last pic, i saw u mention that in another thread, and was hoping someone reliable/honest/unbiased/skilled enough would test it! glad its you!
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: blindingleaf]
#19595803 - 02/20/14 04:57 PM (10 years, 4 days ago) |
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those plates are just the update of what they look like on 2/20 they're the plates I transferred from onto the new plates. So in the picture with less growth the top left one should still be an isolate that just got a needless transfer. Time will tell I guess.
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Sgt. Pepper



Registered: 06/19/13
Posts: 2,538
Loc: Third Stone From The Sun
Last seen: 2 months, 17 days
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: bodhisatta]
#19595869 - 02/20/14 05:08 PM (10 years, 4 days ago) |
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I'm thinking about letting my first plate sit until I see pins and then I'll transfer the pin to a new plate and isolate the fastest sectors. That way I'll have a strong fruiting strain without a lot of testing and a million transfers. Not that I'm being lazy, it just seems like a much better way to do it.
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