Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds High THC Strains   Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Cultivation Supplies   OlympusMyco.com Olympus Myco Polypropylene Grow Bags

Jump to first unread post Pages: < Back | 1 | 2  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Invisible36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,095
Loc: Diving into Mystical Territori...
Trusted Cultivator
Re: Inoculating Grain with an Agar Wedge [Re: FrankHorrigan]
    #19589547 - 02/19/14 11:37 AM (10 years, 4 months ago)

Quote:

FrankHorrigan said:
Quote:

36fuckin5 said:
I use 60mm plates, and I generally use a whole plate for a master jar. As with Frank, though, I never let my cultures touch the edge, and if they happen to hit it, I do another transfer on agar first.




Have you ever had an issue with the cultures hitting the edge? This is one of those things I've read a lot about on paper but never seen in practice.




A couple times, but honestly not that often. Not much more than making transfers from more toward the center.

IME the biggest issue is that you're not using the newest myc possible. That won't cause a problem the first time but it might be the difference between a culture lasting 6 months and 3 years.

Quote:

I always try to use the plates before the cultures hit the edge, but when they do I just cut a bit inside the edge, leaving the outer bit of myc behind on that side.




Same here, but I've drug the edge myc with it before. Sometimes a chain of myc just doesn't wanna split.


--------------------
Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

Extras: Filter Print Post Top
Invisiblelessismore
Registered: 02/10/13
Posts: 6,268
Re: Inoculating Grain with an Agar Wedge [Re: Stromrider]
    #19589591 - 02/19/14 11:50 AM (10 years, 4 months ago)

Quote:

Stromrider said:
Man just do whatever works for you. Their ain't no rules baby. Experiment a little and see what works best for you. That's part of the fun



stammets recommends double wedges (cut 2 and spear them), pretty large, at least ½" or so iirc (TMC)

the more wedges you put in there the faster it will colonize, one small wedge can be terribly slow sometimes with grains

sometimes I use large wedges, sometimes I use smaller ones

depends on if I want to risk my jar, there are usually more mold colonies outside the periphery of the petri dish, due to the dish sucking in contams sometimes when breathing

most people let colonize up to about 1/2 or 2/3, then transfer there

don't transfer to grain with a full colonized plate
don't transfer to grain after you first see mycellium

don't have your petris laying around in your house either, keep them in the glovebox, or they will suck in contams near the periphery of the dish

Extras: Filter Print Post Top
OfflineStromriderM
This must be the place
Male User Gallery


Registered: 06/02/13
Posts: 7,350
Loc: Dept of know what I'm say... Flag
Last seen: 15 days, 9 hours
Trusted Cultivator
Re: Inoculating Grain with an Agar Wedge [Re: FrankHorrigan]
    #19590339 - 02/19/14 03:03 PM (10 years, 4 months ago)

Quote:

FrankHorrigan said:
Quote:

FrankHorrigan said:
Each wedge is another chance for a contam to slip in.




As long as no contams stuck to your agar during inoculation, it will be fine.




Frank are you talking to yourself? :wtf:

Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 5 months
Trusted Cultivator
Re: Inoculating Grain with an Agar Wedge [Re: Stromrider]
    #19590402 - 02/19/14 03:21 PM (10 years, 4 months ago)

frank forgot he's ythan:uhoh:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor

Extras: Filter Print Post Top
InvisiblePirateSwazey
 User Gallery


Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
Trusted Cultivator
Re: Inoculating Grain with an Agar Wedge [Re: Stromrider]
    #19590422 - 02/19/14 03:26 PM (10 years, 4 months ago)

:awesketch:


--------------------
 

BULK RYE PREP  -  MY FAVORITE THREADS  -  BUILD A FLOWHOOD


Extras: Filter Print Post Top
Offlinefractaldill
Cultivator in Training
Registered: 08/31/13
Posts: 909
Last seen: 10 years, 3 months
Re: Inoculating Grain with an Agar Wedge [Re: PirateSwazey]
    #19591304 - 02/19/14 06:38 PM (10 years, 4 months ago)

Well hey! From the sound of it I should be good to go and inoculate a few jars with my Panaelous plates!:crazy2:

Extras: Filter Print Post Top
Offlinefractaldill
Cultivator in Training
Registered: 08/31/13
Posts: 909
Last seen: 10 years, 3 months
Re: Inoculating Grain with an Agar Wedge [Re: RogerRabbit]
    #19591312 - 02/19/14 06:40 PM (10 years, 4 months ago)

Quote:

RogerRabbit said:
A wedge the size of a dime will colonize a quart jar in a week.

Never allow a petri dish to fully colonize.

Quote:

Why does RR insist on one wedge face up?




I don't insist.  I've said the mycelium jumps off slightly faster if right-side-up.  Usually, they flip as they drop so you really don't know how they'll land.  If the agar lands mycelium down, leave it.
RR




I cant see it taking as little as a week. I inoculated 4 jars about 2 weeks ago with fairly large wedges and they still need another week or so.

Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery

Folding@home Statistics
Registered: 04/30/13
Posts: 61,893
Loc: Milky way
Trusted Cultivator
Re: Inoculating Grain with an Agar Wedge [Re: fractaldill]
    #19591371 - 02/19/14 06:50 PM (10 years, 4 months ago)

Quote:

fractaldill said:
Quote:

RogerRabbit said:
A wedge the size of a dime will colonize a quart jar in a week.

Never allow a petri dish to fully colonize.

Quote:

Why does RR insist on one wedge face up?




I don't insist.  I've said the mycelium jumps off slightly faster if right-side-up.  Usually, they flip as they drop so you really don't know how they'll land.  If the agar lands mycelium down, leave it.
RR




I cant see it taking as little as a week. I inoculated 4 jars about 2 weeks ago with fairly large wedges and they still need another week or so.



that has never been my experience even when its like 74F indoors I have never got a grain jar to 100% in a week.

Extras: Filter Print Post Top
InvisibleFrankHorrigan
The Inquisition
Male


Registered: 01/04/11
Posts: 10,573
Trusted Cultivator
Re: Inoculating Grain with an Agar Wedge [Re: 36fuckin5]
    #19594474 - 02/20/14 12:25 PM (10 years, 4 months ago)

Quote:

36fuckin5 said:
IME the biggest issue is that you're not using the newest myc possible. That won't cause a problem the first time but it might be the difference between a culture lasting 6 months and 3 years.




Very true. I was only talking about when I make wedges up for inoculation, not when using the myc for storage or further expansion.


Quote:

Same here, but I've drug the edge myc with it before. Sometimes a chain of myc just doesn't wanna split.




Me too, has it ever caused an issue for you though?

I guess my sterile technique is pretty much spot on now, I haven't seen more than a couple contams that came from my sterile technique in the last few months. If I didn't trust my equipment, setup, and procedures as much as I do, I would be a little more careful about it.\



As for the question of timing, I've seen others get master jars done in a week or so. Vigorous culture, perfectly prepped grains, consistent temps, these are all important. Cubes usually take me 10-12 days to colonize a master, if I shook it a second time in there I bet I could get it down to close to a week. It'd have to be a very young and aggressive isolate though, that's for sure.

Other species can be that fast no problem. Pleurotus ostreatus is a very fast colonizer, I've seen a wedge of my Wellington strain eat a master jar of rye in a week with no shaking at all :eek:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

Extras: Filter Print Post Top
Invisiblelessismore
Registered: 02/10/13
Posts: 6,268
Re: Inoculating Grain with an Agar Wedge [Re: bodhisatta]
    #19594543 - 02/20/14 12:38 PM (10 years, 4 months ago)

Quote:

bodhisatta said:
Quote:

fractaldill said:
Quote:

RogerRabbit said:
A wedge the size of a dime will colonize a quart jar in a week.

Never allow a petri dish to fully colonize.

Quote:

Why does RR insist on one wedge face up?




I don't insist.  I've said the mycelium jumps off slightly faster if right-side-up.  Usually, they flip as they drop so you really don't know how they'll land.  If the agar lands mycelium down, leave it.
RR




I cant see it taking as little as a week. I inoculated 4 jars about 2 weeks ago with fairly large wedges and they still need another week or so.



that has never been my experience even when its like 74F indoors I have never got a grain jar to 100% in a week.



with 1 small wedge it is slow it seems

but with LC it took me 5 days to colonize a grain jar fully :-)

LC is just unneeded risk, use agar for stability, you never know if LC is pure or not, without testing on a few pftek size jars(½-pint)

I think it doesn't matter if it takes 1-2 weeks to colonize personally, you still need to give it an extra week or so to get hold of the jar
so if it colonizes in a week, you let it sit for another week

LC and pftek is very fast too... 1½-2 weeks is the normal for me, but let them sit another week always

Extras: Filter Print Post Top
Invisible36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,095
Loc: Diving into Mystical Territori...
Trusted Cultivator
Re: Inoculating Grain with an Agar Wedge [Re: FrankHorrigan]
    #19596922 - 02/20/14 09:28 PM (10 years, 4 months ago)

Quote:

FrankHorrigan said:
Quote:

Same here, but I've drug the edge myc with it before. Sometimes a chain of myc just doesn't wanna split.




Me too, has it ever caused an issue for you though?




Not that I can recall, no.

Quote:

As for the question of timing, I've seen others get master jars done in a week or so. Vigorous culture, perfectly prepped grains, consistent temps, these are all important. Cubes usually take me 10-12 days to colonize a master, if I shook it a second time in there I bet I could get it down to close to a week. It'd have to be a very young and aggressive isolate though, that's for sure.




I can get jars done in a week or so from g2g, but IDK about from agar. I'd love that strain in my collection, lol.

Quote:

Other species can be that fast no problem. Pleurotus ostreatus is a very fast colonizer, I've seen a wedge of my Wellington strain eat a master jar of rye in a week with no shaking at all :eek:




Same here. Oysters are insane.


--------------------
Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.

Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2  [ show all ]

Shop: Original Sensible Seeds High THC Strains   Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Cultivation Supplies   OlympusMyco.com Olympus Myco Polypropylene Grow Bags


Similar ThreadsPosterViewsRepliesLast post
* Innoculating with agar wedge observations homebrew 3,564 4 03/01/02 08:00 AM
by homebrew
* inoculation using Agar infamous 600 4 01/16/05 08:43 AM
by ohmatic
* Innoculation with agar wedge Visigoth 5,439 6 01/15/23 12:59 PM
by Screwup
* Agar Wedge / Mushroom Tissue to mycelium syringe - ohm's ghetto tek
( 1 2 all )
ohmatic 8,733 22 01/11/05 04:50 AM
by ohmatic
* Spawn to inoculate grain? lumpus 751 1 05/10/03 11:10 AM
by MykeOfile
* WBS jar stalling after agar inoculation (photo) Josborne 944 14 05/04/21 02:29 PM
by Stipe-n Cap
* Pins on agar and one with contaminant TripPSmittens 1,835 10 05/07/21 09:45 AM
by TripPSmittens
* Grain to Grain kindkesey 2,342 13 03/03/04 07:50 PM
by Anonymous

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
8,194 topic views. 16 members, 76 guests and 145 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.028 seconds spending 0.009 seconds on 12 queries.