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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,095
Loc: Diving into Mystical Territori...
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Re: Inoculating Grain with an Agar Wedge [Re: FrankHorrigan]
#19589547 - 02/19/14 11:37 AM (10 years, 4 months ago) |
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FrankHorrigan said:
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36fuckin5 said: I use 60mm plates, and I generally use a whole plate for a master jar. As with Frank, though, I never let my cultures touch the edge, and if they happen to hit it, I do another transfer on agar first.
Have you ever had an issue with the cultures hitting the edge? This is one of those things I've read a lot about on paper but never seen in practice.
A couple times, but honestly not that often. Not much more than making transfers from more toward the center.
IME the biggest issue is that you're not using the newest myc possible. That won't cause a problem the first time but it might be the difference between a culture lasting 6 months and 3 years.
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I always try to use the plates before the cultures hit the edge, but when they do I just cut a bit inside the edge, leaving the outer bit of myc behind on that side.
Same here, but I've drug the edge myc with it before. Sometimes a chain of myc just doesn't wanna split.
-------------------- Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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lessismore
Registered: 02/10/13
Posts: 6,268
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Re: Inoculating Grain with an Agar Wedge [Re: Stromrider]
#19589591 - 02/19/14 11:50 AM (10 years, 4 months ago) |
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Stromrider said: Man just do whatever works for you. Their ain't no rules baby. Experiment a little and see what works best for you. That's part of the fun
stammets recommends double wedges (cut 2 and spear them), pretty large, at least ½" or so iirc (TMC)
the more wedges you put in there the faster it will colonize, one small wedge can be terribly slow sometimes with grains
sometimes I use large wedges, sometimes I use smaller ones
depends on if I want to risk my jar, there are usually more mold colonies outside the periphery of the petri dish, due to the dish sucking in contams sometimes when breathing
most people let colonize up to about 1/2 or 2/3, then transfer there
don't transfer to grain with a full colonized plate don't transfer to grain after you first see mycellium
don't have your petris laying around in your house either, keep them in the glovebox, or they will suck in contams near the periphery of the dish
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,350
Loc: Dept of know what I'm say...
Last seen: 15 days, 9 hours
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Re: Inoculating Grain with an Agar Wedge [Re: FrankHorrigan]
#19590339 - 02/19/14 03:03 PM (10 years, 4 months ago) |
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FrankHorrigan said:
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FrankHorrigan said: Each wedge is another chance for a contam to slip in.
As long as no contams stuck to your agar during inoculation, it will be fine.
Frank are you talking to yourself?
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 5 months
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Re: Inoculating Grain with an Agar Wedge [Re: Stromrider]
#19590402 - 02/19/14 03:21 PM (10 years, 4 months ago) |
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frank forgot he's ythan
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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PirateSwazey



Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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Re: Inoculating Grain with an Agar Wedge [Re: Stromrider]
#19590422 - 02/19/14 03:26 PM (10 years, 4 months ago) |
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fractaldill
Cultivator in Training
Registered: 08/31/13
Posts: 909
Last seen: 10 years, 3 months
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Re: Inoculating Grain with an Agar Wedge [Re: PirateSwazey]
#19591304 - 02/19/14 06:38 PM (10 years, 4 months ago) |
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Well hey! From the sound of it I should be good to go and inoculate a few jars with my Panaelous plates!
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fractaldill
Cultivator in Training
Registered: 08/31/13
Posts: 909
Last seen: 10 years, 3 months
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Re: Inoculating Grain with an Agar Wedge [Re: RogerRabbit]
#19591312 - 02/19/14 06:40 PM (10 years, 4 months ago) |
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RogerRabbit said: A wedge the size of a dime will colonize a quart jar in a week.
Never allow a petri dish to fully colonize.
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Why does RR insist on one wedge face up?
I don't insist. I've said the mycelium jumps off slightly faster if right-side-up. Usually, they flip as they drop so you really don't know how they'll land. If the agar lands mycelium down, leave it. RR
I cant see it taking as little as a week. I inoculated 4 jars about 2 weeks ago with fairly large wedges and they still need another week or so.
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,893
Loc: Milky way
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Re: Inoculating Grain with an Agar Wedge [Re: fractaldill]
#19591371 - 02/19/14 06:50 PM (10 years, 4 months ago) |
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fractaldill said:
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RogerRabbit said: A wedge the size of a dime will colonize a quart jar in a week.
Never allow a petri dish to fully colonize.
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Why does RR insist on one wedge face up?
I don't insist. I've said the mycelium jumps off slightly faster if right-side-up. Usually, they flip as they drop so you really don't know how they'll land. If the agar lands mycelium down, leave it. RR
I cant see it taking as little as a week. I inoculated 4 jars about 2 weeks ago with fairly large wedges and they still need another week or so.
that has never been my experience even when its like 74F indoors I have never got a grain jar to 100% in a week.
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Inoculating Grain with an Agar Wedge [Re: 36fuckin5]
#19594474 - 02/20/14 12:25 PM (10 years, 4 months ago) |
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36fuckin5 said: IME the biggest issue is that you're not using the newest myc possible. That won't cause a problem the first time but it might be the difference between a culture lasting 6 months and 3 years.
Very true. I was only talking about when I make wedges up for inoculation, not when using the myc for storage or further expansion.
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Same here, but I've drug the edge myc with it before. Sometimes a chain of myc just doesn't wanna split.
Me too, has it ever caused an issue for you though?
I guess my sterile technique is pretty much spot on now, I haven't seen more than a couple contams that came from my sterile technique in the last few months. If I didn't trust my equipment, setup, and procedures as much as I do, I would be a little more careful about it.\
As for the question of timing, I've seen others get master jars done in a week or so. Vigorous culture, perfectly prepped grains, consistent temps, these are all important. Cubes usually take me 10-12 days to colonize a master, if I shook it a second time in there I bet I could get it down to close to a week. It'd have to be a very young and aggressive isolate though, that's for sure. Other species can be that fast no problem. Pleurotus ostreatus is a very fast colonizer, I've seen a wedge of my Wellington strain eat a master jar of rye in a week with no shaking at all
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lessismore
Registered: 02/10/13
Posts: 6,268
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Re: Inoculating Grain with an Agar Wedge [Re: bodhisatta]
#19594543 - 02/20/14 12:38 PM (10 years, 4 months ago) |
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bodhisatta said:
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fractaldill said:
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RogerRabbit said: A wedge the size of a dime will colonize a quart jar in a week.
Never allow a petri dish to fully colonize.
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Why does RR insist on one wedge face up?
I don't insist. I've said the mycelium jumps off slightly faster if right-side-up. Usually, they flip as they drop so you really don't know how they'll land. If the agar lands mycelium down, leave it. RR
I cant see it taking as little as a week. I inoculated 4 jars about 2 weeks ago with fairly large wedges and they still need another week or so.
that has never been my experience even when its like 74F indoors I have never got a grain jar to 100% in a week.
with 1 small wedge it is slow it seems
but with LC it took me 5 days to colonize a grain jar fully :-)
LC is just unneeded risk, use agar for stability, you never know if LC is pure or not, without testing on a few pftek size jars(½-pint)
I think it doesn't matter if it takes 1-2 weeks to colonize personally, you still need to give it an extra week or so to get hold of the jar so if it colonizes in a week, you let it sit for another week
LC and pftek is very fast too... 1½-2 weeks is the normal for me, but let them sit another week always
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,095
Loc: Diving into Mystical Territori...
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Re: Inoculating Grain with an Agar Wedge [Re: FrankHorrigan]
#19596922 - 02/20/14 09:28 PM (10 years, 4 months ago) |
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Quote:
FrankHorrigan said:
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Same here, but I've drug the edge myc with it before. Sometimes a chain of myc just doesn't wanna split.
Me too, has it ever caused an issue for you though?
Not that I can recall, no.
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As for the question of timing, I've seen others get master jars done in a week or so. Vigorous culture, perfectly prepped grains, consistent temps, these are all important. Cubes usually take me 10-12 days to colonize a master, if I shook it a second time in there I bet I could get it down to close to a week. It'd have to be a very young and aggressive isolate though, that's for sure.
I can get jars done in a week or so from g2g, but IDK about from agar. I'd love that strain in my collection, lol.
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Other species can be that fast no problem. Pleurotus ostreatus is a very fast colonizer, I've seen a wedge of my Wellington strain eat a master jar of rye in a week with no shaking at all 
Same here. Oysters are insane.
-------------------- Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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