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InvisibleMushmitch
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Re: Rye lime soak.. [Re: Mushmitch]
    #19436177 - 01/18/14 02:22 AM (10 years, 1 month ago)

UPDATED AT TOP.


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OfflineOICU812
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Re: Rye lime soak.. [Re: Mushmitch]
    #19436510 - 01/18/14 06:10 AM (10 years, 1 month ago)

Quote:

Mushmitch said:
UPDATED AT TOP.




It would help us followers of your thread if you would include a date beside each update as there seems to be several at top and the timeline gets a bit lost.

Just My 2ยข


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"Democracy is two wolves and a lamb voting on what to have for lunch. Liberty is a well-armed lamb contesting the vote!" --Benjamin Franklin

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InvisibleMushmitch
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Re: Rye lime soak.. [Re: OICU812]
    #19458435 - 01/22/14 04:20 PM (10 years, 1 month ago)

Any comments?

I just spawned to no pc'd rye seed and no one thinks that's awesome ?? :laugh:


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InvisibleGanzig
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Re: Rye lime soak.. [Re: Mushmitch]
    #19458692 - 01/22/14 05:05 PM (10 years, 1 month ago)

:curbyourenthusiasm:

Dude for sure! That is awesome.

:fuckyeahdance:


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Offlinecurry
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Re: Rye lime soak.. [Re: Ganzig]
    #19463656 - 01/23/14 05:21 PM (10 years, 1 month ago)

Nice job.  I'm all about cutting out unnecessary steps, if it'll work another way.  You can get away with all kinds of things in the winter that you can't in the summer... so why not?  Trich will eat your lunch in the summertime, so you have to do it by the book then... but while it's winter- experiment.


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Invisiblesolarity
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Re: Rye lime soak.. [Re: curry]
    #19466648 - 01/24/14 09:11 AM (10 years, 1 month ago)

Ground breaking stuff - thanks for doing the work and documenting so well!

It always surprises me on here when people say "just dont bother trying" to anyone with a new idea. If the result is positive then it will still need to be duplicated by many others to prove it's worth - the same should apply to a negative result.

When I get a few mins I will try a similar experiment with wheat grains. Hot alkaline solution at 12ph should kill a few things! Only issue will be working out how to drain in front of the hood.


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InvisibleAleon
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Re: Rye lime soak.. [Re: solarity]
    #19467849 - 01/24/14 02:02 PM (10 years, 1 month ago)

Quote:

solarity said:
Ground breaking stuff - thanks for doing the work and documenting so well!

It always surprises me on here when people say "just dont bother trying" to anyone with a new idea. If the result is positive then it will still need to be duplicated by many others to prove it's worth - the same should apply to a negative result.

When I get a few mins I will try a similar experiment with wheat grains. Hot alkaline solution at 12ph should kill a few things! Only issue will be working out how to drain in front of the hood.




If the grains are soaked thoroughly, you may not have to do it in front of the hood.


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Offlinechefinainteasy
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Re: Rye lime soak.. [Re: Aleon]
    #19478919 - 01/26/14 09:04 PM (10 years, 29 days ago)

So I thought I would give this a go since I have no lab and hood is packed away. I used white millet as my grain.

First I soaked the millet for 24 hours in tap water only(1-24)

Dry weight was 2419g

I then drained and weighed it to get a sense of how much water this grain can take in(1-25)

3334.7g after soaking

It then sat in a container for a day because I was to busy yesterday to experiment. So today,(1-26) I did my cooking and inoculating.

-First I put the grains in the pot of water and filled it with 12ph water, about 6L. I let it sit and soak for 2 hours. After that time I checked the ph of the water. It had dropped down to 8.5. I would assume the grain was slightly acidic from soaking and sitting for a day. I then added 4g hydrated lime and 16g gypsum and brought the ph back up to 12.5. I then heated the pot to a boil, reduced the heat and simmered for 10 minutes.


I then drained for about 10 minutes


Then spread out on a sheet tray lined with paper towels to cool and dry.


This bag is 1000g of the cooked millet and 100g of F.F's Dove Grey spawn that was made 2-13 of last year.


I filled a large filter patch bag with 1950g millet and 500g rye.
Also just for fun I filled another small filter bag with 694g millet and dropped a chopped agar dish. The culture was a T3 Dove Grey made 12/30/2012(yes that old)

If I had younger spawn or cultures I would certainly of used them, but I don't. I figured if I get any results from this it will be that much more amazing. But I'm not that hopeful. Open air grain to grain, and agar to grain cant be this easy or people would be doing it. But worth a shot since I've got nothing else to do right now.


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OfflineRogerRabbitM
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Re: No PC Rye lime soak.. UPDATED 1/18/14 [Re: Mushmitch]
    #19484861 - 01/28/14 05:41 AM (10 years, 27 days ago)

Quote:

Mushmitch said:

Looks to be a success!!!!1/16


These pics were from 3 days ago. I'm gonna spawn to bag of straw!!




That's some major bacteria contamination.  I wouldn't declare success.

Also, if you're going to update a thread, do so at the end of the thread, not the start.  If a thread is five or six pages long, we only want to open the last page to follow.
RR


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Offlinechefinainteasy
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Re: No PC Rye lime soak.. UPDATED 1/18/14 [Re: RogerRabbit]
    #19517960 - 02/03/14 08:59 PM (10 years, 21 days ago)

http://www.shroomery.org/forums/showflat.php/Number/19517944/vc/1#19517944
Updated pictures of the millet bags. The one with the agar wedge contaminated, the other two are fully colonized without viable contamination.


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Offlineghiajake
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Re: No PC Rye lime soak.. UPDATED 1/18/14 [Re: chefinainteasy]
    #19518210 - 02/03/14 09:52 PM (10 years, 21 days ago)

This is a great idea MM. I have been playing around with lime soaking grains before PCing to help fight contams and have seemed to have good success. I'm gonna try this with some red milo and spawn it with an extra Enoki jar I have left from an LC test.

BTW, if that's the Yellow Oyster culture you got from me, they are Pearls not Yellows. The Blue Oyster culture I got from the same guy was mislabeled too, it turned out to be King Oysters. I'll post pics in a bit, once I re-up my Supporter status so I can post pics again.


Edited by ghiajake (02/03/14 10:04 PM)


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Offlineghiajake
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Re: No PC Rye lime soak.. UPDATED 1/18/14 [Re: ghiajake]
    #19518314 - 02/03/14 10:13 PM (10 years, 20 days ago)

Here's those pics. The last one is of the Kings that were supposed to be Blues.



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Invisiblelipa

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Re: No PC Rye lime soak.. UPDATED 1/18/14 [Re: ghiajake]
    #19520146 - 02/04/14 12:11 PM (10 years, 20 days ago)

Quote:

ghiajake said:
Here's those pics. The last one is of the Kings that were supposed to be Blues.






What species is that pale oyster? They look like they have a depression on top.  Is it Pleurotus cornucopiae?


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Offlineghiajake
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Re: No PC Rye lime soak.. UPDATED 1/18/14 [Re: lipa]
    #19521067 - 02/04/14 03:44 PM (10 years, 20 days ago)

Quote:

lipa said:
What species is that pale oyster? They look like they have a depression on top.  Is it Pleurotus cornucopiae?




To be honest lipa, I'm not sure. They were supposed to have been P. citripileatus, but definitely are not. They seemed to grow better in colder temps. The top-fruited ones were fruited at 55-60F, and the side-fruited ones were done at 70F. I did pick the side-fruited ones early because my ultrasonic crapped out. I am assuming the are Pearls, but definitely could use help in ID'ing them. I know for a fact the what was supposed to have been the Blue Oyster culture is in fact King Oyster.


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Invisiblesolarity
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Re: No PC Rye lime soak.. UPDATED 1/18/14 [Re: ghiajake]
    #19521461 - 02/04/14 05:03 PM (10 years, 20 days ago)

1st and 3rd pic looks exactly like Pseudo Hyp. Ulm. to me.
2nd and 4th pics are what I would assume it would look like if picked too late.


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Offlineghiajake
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Re: No PC Rye lime soak.. UPDATED 1/18/14 [Re: solarity]
    #19521525 - 02/04/14 05:16 PM (10 years, 20 days ago)

That's what I though, but everything I read stated that the pseudo Hyp Ulm was most-likely Pearl Oyster. These are obviously not true Hyp Ulm.


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Invisiblesolarity
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Re: No PC Rye lime soak.. UPDATED 1/18/14 [Re: ghiajake]
    #19524469 - 02/05/14 09:54 AM (10 years, 19 days ago)

There is a whole other thread on this but I can confirm that (pseudo) HU is not Pleur. Ost. Huge difference in tolerance for light and CO2, also different taste, texture and shelf life. Having said that "Pearl Oyster" is a perfectly good name for it!


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Offlineghiajake
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Re: No PC Rye lime soak.. UPDATED 1/18/14 [Re: solarity]
    #19525539 - 02/05/14 01:46 PM (10 years, 19 days ago)

Either way, I wasn't too upset about the mix up of cultures. I didn't have any like these yet. The supposed Blues were a little more upsetting because I already have an Aloha strain of Kings, but I haven't successfully fruited those yet. I have a dish of verified Blues on its way, so no biggie. Just have to get some real Yellows before spring. :smile:


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Offlinecmspice
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Re: No PC Rye lime soak.. UPDATED 1/18/14 [Re: ghiajake]
    #19553782 - 02/11/14 06:18 PM (10 years, 13 days ago)

Pretty cool stuff! I wonder if it's bacterial fermentation dropping the ph leading way for the mycelium to take over.


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Offlinechefinainteasy
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Re: No PC Rye lime soak.. UPDATED 1/18/14 [Re: cmspice]
    #19553911 - 02/11/14 06:44 PM (10 years, 13 days ago)

That's what I suspect. There is another post by Mycelo using that very process. Im starting another batch now. Just spawned the last batch to bulk and things are moving along well!


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