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Chowder
Human


Registered: 10/26/09
Posts: 256
Last seen: 4 years, 1 month
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Did I just fail my first rye tek?
#19504915 - 02/01/14 06:57 AM (10 years, 11 months ago) |
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So:
I got 7 quart jars with rye. Followed RR tek, soak for ~20 hrs, simmer for 10 mins, dry them properly, did the TP test. Only PC'd 1 day later, 12PSI for 120-130 mins.
I spent a lot of time before, preparing the lids, two holes. One with a SFD disk, the other with a bought injection port. I wanted my jars to look nice and neat, BAD IDEA!
I did my inoculation in a SAB, with proper sanitation. I kinda had the feeling that the needle would bent, so I sterilized another spare needle, just in case.
First jar, red hot needle, it bent right away, not even a poke in this crappy injection port. Right, change the needle, same thing! Not a chance..
I didn't know what to do, so I took out the needle all together, sanitized the area around the nib with ISO, and with quick movements I opened the lids and squirted the spores straight in the jar. Trying to sanitize my hand the the nib as many times as possible
What do you think are my chances of success?
I have another 7 jars waiting to be PC'd tonight. What should I do? Try and quickly find a better needle for my syringe? Or should I do the same technique? Or should I just remove the rubber injection ports and make some RTV ones instead.
Also, can I just store the jars on a shelf? Or darkness is better? ( I fear that sunlight is going to form condensation.
PS: Sorry for being a paranoid pest around there.. Thanks for your time.
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Quexl

Registered: 12/17/13
Posts: 1,443
Loc:
Last seen: 9 years, 10 months
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Re: Did I just fail my first rye tek? [Re: Chowder]
#19504996 - 02/01/14 07:35 AM (10 years, 11 months ago) |
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Quote:
Chowder said: One with a SFD disk, the other with a bought injection port. I wanted my jars to look nice and neat, BAD IDEA!
I think it's a good idea! I've never used standard ports but I have a feeling they weren't meant to take the size of needle that we use for inoculations. I've never had a problem with ones made of gasket sealant, you may find them easier to deal with.
Quote:
I did my inoculation in a SAB, with proper sanitation.
This is at least somewhat similar to a G2G in a SAB, so as long as everything went smooth you shouldn't have contamination. The (sort of) plus side is that if you did contaminate it, you'll probably know fairly quickly.
Quote:
...should I just remove the rubber injection ports and make some RTV ones instead.
I think so. But again, I've never used rubber pre-made ones. I have a feeling that they're either way too thick, or if they look like what I think they look like, after you use them you'll leave a hole behind. Gasket sealant is about 5$ and lasts for a good 12+ holes. Downside is you'll have to wait another day or so for it to harden.
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Mr.Colorado
chef extraordinaire



Registered: 11/18/13
Posts: 16
Loc: not Colorado
Last seen: 5 years, 6 months
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Re: Did I just fail my first rye tek? [Re: Quexl]
#19505065 - 02/01/14 08:00 AM (10 years, 11 months ago) |
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I never understood all those fancy lid teks/ideas. The simpler the better, imho... tyvek has yet to fail me. If your sterile game is proper, as you claim it to be, you'll probably be o.k. Just a waiting game now though.... Best of luck!
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Did I just fail my first rye tek? [Re: Mr.Colorado]
#19505140 - 02/01/14 08:26 AM (10 years, 11 months ago) |
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in the few cases i use a syringe, its in an SAB of course, but i just unscrew the lid and inject a little, quickly, and screw back on, use like u would for a G2G, but its faster/easier because lid is only cracked open for a few seconds while 3-4 drops come out of needle, then closed. be it spore syringe or lc. no need to add another thing (the port whether its pre made or RTV) as a contamination vector and have to try and sanitize it (with possibly missing parts of that inoculation port with the iso or bleach or whatever u use)
im not sure i follow what u did. if u meant u took the metal part off the needle that screws on because it bent, and used the bigger part that holds everything, u might be ok because u did it in an sab...only time will tell
yea just store them on shelf with no worries. light or no light, whatever ur situation allows for.
-------------------- A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!
The whole is greater than the sum of its parts
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,097
Loc: Diving into Mystical Territori...
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Re: Did I just fail my first rye tek? [Re: blindingleaf]
#19505309 - 02/01/14 09:06 AM (10 years, 11 months ago) |
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You should be fine. I do it the same way whenever using syringes because I don't use injection ports, only SFD's, and only whole.
-------------------- Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.
bodhisatta said:
i recommend common sense and figuring it out.
These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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1down5up
Social Ninja



Registered: 11/04/13
Posts: 1,743
Last seen: 7 months, 2 days
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Re: Did I just fail my first rye tek? [Re: 36fuckin5]
#19505342 - 02/01/14 09:18 AM (10 years, 11 months ago) |
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teh only problem i've had with the SHIP is when i pull the needle back out. If you don't use enough sealant, it might pull off. I hold the port with my other hand while i pull the needle out. Do you wait till your needle cools before you try to inject them?
-------------------- ~~Everything is relative~~
A Simplified Overview of Mushroom Cultivation Strategies - RR says - EvilMushroom666's Take on BRF Cakes - Frank's list of goodies - Cronicr's Goodies
No one is placed higher than another no matter race or creed or gender, we must teach forgiveness and compassion for all life. J.L.
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monoculture
Mycaddict



Registered: 10/16/13
Posts: 246
Last seen: 10 years, 5 months
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Re: Did I just fail my first rye tek? [Re: 1down5up]
#19505417 - 02/01/14 09:43 AM (10 years, 11 months ago) |
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I use those inoculation ports and never had a bent needle because of them. It's either your needles or the angle of injection. This is one of my lids:

I even fill the hole on the inside of the injection port with silicone to make the self healing better because that sucks a bit for butyl normally.
Penetrating them does cost more power than a silicone port, but gives no problems for me. Just inject them straight in the center perfectly downwards at an angle of 90 degrees. When you poked trough, go the direction you want to go and insert the needle further. Works for me every time and I do 24 cakes at once with 4 inoc holes each, so that's 96 times in a row with 2 syringes. So my needles penetrate such a butyl stopper like 50 times in a row without bending. The needles I use are 0,90 x 40 mm, G20.
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Sgt. Pepper



Registered: 06/19/13
Posts: 2,551
Loc: Third Stone From The Sun
Last seen: 3 months, 15 days
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Re: Did I just fail my first rye tek? [Re: monoculture]
#19505527 - 02/01/14 10:15 AM (10 years, 11 months ago) |
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RTV is great, a $7 tube lasts for at least 24 jars and I've never had one fail. I use it to make injection ports for my pp5 agar too. It keeps everything neat and sterile and I hate working on jars without them. To each their own though.
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Chowder
Human


Registered: 10/26/09
Posts: 256
Last seen: 4 years, 1 month
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Re: Did I just fail my first rye tek? [Re: Sgt. Pepper]
#19508650 - 02/01/14 11:12 PM (10 years, 11 months ago) |
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Thanks for the good replies guys.
Indeed I did not wait for too long for the needle to cool down, its because I didnt want contams to fall on it.
Also, it's not about the angle of the injection, I did make sure to make it 90 degrees, but the needle was just not sharp enough.
I might just have to take the standard injection ports out and make a big ball of RTV.
Thanks again!
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Quexl

Registered: 12/17/13
Posts: 1,443
Loc:
Last seen: 9 years, 10 months
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Re: Did I just fail my first rye tek? [Re: Chowder]
#19508762 - 02/01/14 11:47 PM (10 years, 11 months ago) |
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Quote:
Chowder said: I did not wait for too long for the needle to cool down, its because I didnt want contams to fall on it
You were right to inject while hot. The syringe is cooled by the time you replace it's volume once or twice. It's a very small amount.
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1down5up
Social Ninja



Registered: 11/04/13
Posts: 1,743
Last seen: 7 months, 2 days
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Re: Did I just fail my first rye tek? [Re: Quexl]
#19509526 - 02/02/14 05:58 AM (10 years, 11 months ago) |
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If you inject in a SAB or glovebox, you shouldn't have to worry about anything falling on the needle. Injecting the needle while it's still hot can damage the IP's.
-------------------- ~~Everything is relative~~
A Simplified Overview of Mushroom Cultivation Strategies - RR says - EvilMushroom666's Take on BRF Cakes - Frank's list of goodies - Cronicr's Goodies
No one is placed higher than another no matter race or creed or gender, we must teach forgiveness and compassion for all life. J.L.
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