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Invisibleagarmeister
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Registered: 01/10/14
Posts: 278
Extremely slow colonisation
    #19509124 - 02/02/14 02:12 AM (10 years, 11 months ago)

Hi Guys, it's my first post after months of lurking, searching and reading. So, hello and thanks for everything so far :wink:

I've been trying to grow for four months now without much luck, using a number of spore prints.

Here's what I've done so far:

1. Spores to Agar, it germinated fine, in about A week I could start seeing the myc. Agar is mixed with a bit of liquid malt extract.

2. Isolated strains, discarded contaminated dishes, ended up with some nice ryzomorphic shapes which didn't contaminate.

3. prepared organic rye grains per RR, made about 12 jars of various sizes, filtered with micropore tape and polyfill.

Now I have 10 jars (2 were discarded due to contamination) with some myc grow. it's been about 3 weeks and it's still not much bigger than the initial agar wedge originally inserted to the jar. It does grow, but in a pace which will take months to fully colonize.

I tried shaking some of the jars, and also changed the filter of a few others to one layer of new micropore (without polyfill) just in case that there needs to be more gas exchange.

I'm doing everything as explained by RR, the spores were purchased from a reliable shop.

Any ideas?

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OfflinecronicrMFacebook
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Registered: 08/07/11
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Re: Extremely slow colonisation [Re: agarmeister]
    #19509146 - 02/02/14 02:26 AM (10 years, 11 months ago)

post a pic:shrug:


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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InvisibleDr.Dankhead
Uhh...doctor gonzo?
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Registered: 03/29/13
Posts: 5,187
Loc: Breathing down your neck Flag
Re: Extremely slow colonisation [Re: cronicr]
    #19509151 - 02/02/14 02:28 AM (10 years, 11 months ago)

Quote:

cronicr said:
post a pic:shrug:




You run this forum cron. All day...


Shit you run this website:thumbup:


--------------------

:mushroom2:**need a check up?**:aliendance: **im a Doctor**:mushroom2:
:bow2::bow2::bow2::bow2::bow2::bow2::bow2::bow2::bow2::bow2:
i sometimes wish I was a wormy, wiggling all in the cold dirt...tickle tackle pickle dickle think a mackshift thought of broken words broken gears and words of conundrums..I'm not a weiner doctor so take that shit to dr. Gonz free boob inplant consultations.. Photo required

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OfflineValyr
Con nulla non si fa nulla.

Registered: 05/08/13
Posts: 381
Last seen: 7 years, 8 months
Re: Extremely slow colonisation [Re: cronicr]
    #19509156 - 02/02/14 02:32 AM (10 years, 11 months ago)

pics!!!. Pics will let us know if its even mush myc. IF your grains were too wet, burst etc. Or any number of other things. Also what temp are your jars kept at?

Dont know why im even bothering to type this since the guy I go to for questions is already here but hey what the heck lol (cron).

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OfflinecronicrMFacebook
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Re: Extremely slow colonisation [Re: Dr.Dankhead]
    #19509159 - 02/02/14 02:33 AM (10 years, 11 months ago)

Quote:

Dr.Dankhead said:
Quote:

cronicr said:
post a pic:shrug:




You runTROLL this forum cron. All day...


Shit you runTROLL this website:thumbup:



:uhoh::laugh2:


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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OfflineValyr
Con nulla non si fa nulla.

Registered: 05/08/13
Posts: 381
Last seen: 7 years, 8 months
Re: Extremely slow colonisation [Re: cronicr]
    #19509174 - 02/02/14 02:37 AM (10 years, 11 months ago)

:pimp: lol

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Invisibleagarmeister
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Registered: 01/10/14
Posts: 278
Re: Extremely slow colonisation [Re: Dr.Dankhead]
    #19509183 - 02/02/14 02:43 AM (10 years, 11 months ago)

I will try taking some pictures and post here (I don't have a proper camera so I will use my puny webcam)

Until then, are there any causes for slow growth other than low gas exchange, (invisible) contamination or too dry / wet grains?

Also, local temp. at this time is about 63 - 71

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OfflinecronicrMFacebook
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Re: Extremely slow colonisation [Re: agarmeister]
    #19509188 - 02/02/14 02:45 AM (10 years, 11 months ago)

sounds like a contam, i'm sure you would know if your grains were to dry and if there too wet...it's probably a contam


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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Invisibleagarmeister
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Registered: 01/10/14
Posts: 278
Re: Extremely slow colonisation [Re: cronicr]
    #19509200 - 02/02/14 02:51 AM (10 years, 11 months ago)

A number out of the initial batch of jars did get contaminated, and when it happens, there's no mistaking it. (It was Trich)

Is is possible that ALL of my other jars are contaminated, and that there's no way to spot it by the naked eye?

I looked all over the forums for contamination pics and saw none of that on my own jars.

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OfflinecronicrMFacebook
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Registered: 08/07/11
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Re: Extremely slow colonisation [Re: agarmeister]
    #19509204 - 02/02/14 02:53 AM (10 years, 11 months ago)

i would just star over, 3 weeks they should be finished


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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