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Offlinethatmonk
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Registered: 09/27/13
Posts: 160
Loc: Near Astoria, OR
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Grow Plan and Log for Panaeolus Cyanescens
    #19508826 - 02/02/14 12:07 AM (10 years, 11 months ago)

After success in growing and voyaging on B+ and Ecuador I am moving onto Panaeolus Cyanescens: agar to WBS to H-Poo. Given all the different teks and approaches I decided this time to write a proper Grow Plan to help me internalize my understanding and so I wouldn't forget any important steps. Please review the grow plan and add any comments below if you like. Especially if there are any problems or mistakes in the plan.

Grow Plan for Panaeolus Cyanescens
Spore to Agar to WBS to H-Poo

for 20 cups substrate in 5 cup 6.5” diameter X 3.5” deep plastic containers.
By: ThatMonk


4c dry WBS = 5 cup soaked
15c substrate plus 5c spawn equals 20c Final substrate

1. Put on favorite entheogenic music.
a. Blueteck, Spongle, Gaiser or Matmos

2. Prepare Mycomedia Antibiotic Malt Extract Agar into six petri dishes
a. Mix 5 grams agar in 0.1 liters hot water in canning jar.
b. Pour agar into Petri dish.
c.  Pressure cook for 10 minutes at 15psi.
c. Cool PC under hot, then cool water spray in sink.
d. Remove Petri dishes to cool.

3. Prepare Myself
a. Eat some food and drink some water.
b. Take a shower and wash well.
c. Put on clean cloths.
d. Put on cap
e. Put on mask
f. Wash hands, fingernails and forearms well.
g. Put on gloves
h. Rub alcohol on gloves and air dry.

4. Prepare glove box
a. Wipe down interior and gloves with isopropyl alcohol and dry with paper towel.
b. Wipe work surface and floor in laundry room with Lysol.
c. Place glove box on work surface in laundry room.
d. Set up glove box lighting.
e. Lightly spray interior of glove box with Lysol. Place lid and let sit for five minutes.
f. Spray laundry room well with Lysol and wait five minutes.
g. Wipe down petri dishes with alcohol and place in glove box.
h. Shake well the spore syringe.
i. Wipe down spore syringes with alcohol and place in glove box.
j. Wipe down scissors with alcohol and place in glove box.
k. Place Parafilm in glove box.
l. Set up alcohol burner next to glove box tool sterilization port and light after alcohol is dry.

5. Inoculate Petri Dishes
a. Shake spore syringe again.
b. Remove cap, attach needle.
c. Flame sterilize needle.
d. Place one drop of spore solution in each dish.
e. Apply Parafilm seal on edge of Petri dish.
f. Place in incubator at 80 degrees

6. Prepare WBS Spawn
a. When cultures are ready:
b. Put 3c dry Wild Bird Seed in 12c water and simmer for 30-40 minutes, then let soak in water for 12 hours.
c. Drain WBS in colander, rinse well in cold water for 30 minutes until no water comes out.
d. In large bowl add 1.5c vermiculite
e. Load prepared WBS into four 1/2p mason jars. 1c per jar.
f. Add ½” to ¾” layer of vermiculite as your contamination barrier.
g. Apply lid with four 1/8” holes and screw ring down snug.
h. Label each container with a number, record notes in grow log.
i. Apply single foil lid.
j. Pressure cook for 90 minutes at 15psi.
k. Cool PC in sink using hot water spray, then cool water spray until cool.
l. Remove jars and cool to room temperature.

7. Inoculate WBS Spawn
a. Write inoculation date and mushroom species and race on jar sticker. Make notes in grow log.
b. Prepare self as in step 3.
c. Prepare glove box as in step 4.
d. Pick best looking culture.
e. Prep colonized Petri dish, sterile syringe and needle and sterile water with alcohol and place in glove box.
f. Prep alcohol burner near tool sterilizing port and light.
g. Inject 10cc sterile water into mycelium in Petri dish and mix slurry with tip of needle. Avoid cutting into the agar.
h. Draw slurry into syringe.
i. Flame sterilize needle.
j. Remove foil lid.
k. Inject 2cc (0.5cc per port) into WBS 1/2p spawn jars through filter mediums.
l. Remove from glove box and place in incubator at 80 degrees F.

8. Prepare Horse Poo Substrate
a. Put 15c fresh field picked H-poo in a cloth bag.
b. Bring water to a boil, then cool to 170 degrees.
c. Pasteurize at 170 degrees F for 40 minutes in water.
d. Remove and cool in alcohol wiped and dried Rubbermaid .
e. Hand squeeze to drain and let drain for 1 hours in colander. Should be moist, not wet.
f. Place H-poo in four 5c PP5 containers

9. Inoculate H-Poo Substrate
a. Prepare lids with 8 x 1/8” holes with drill or soldering iron.
b. Clean lids and containers with alcohol and air dry.
c. Prepare self as in step 3
d. Prepare laundry room as in step 4.
e. Put on gloves, rub with alcohol and air dry.
f. Take one 1/2p jar of WBS spawn and mix well with one container of H-poo.
g. Apply Tyvek filter
h. Apply lids.
i. Place in incubator at 80 degrees F.

10. Apply Casing.
a. Prepare self as in step 3
b. Prepare laundry room as in step 4.
c. Remove lids and add 3/8” layer dry fine vermiculite.
d. Spray vermiculite to field capacity.
e. Replace filter and lid.
f. Return to incubation for 2-3 days.

11. Fruit Substrate
a. Prepare self as in step 3
b. Prepare SGFC.
c. Remove lids from substrate containers
d. Place in SGFC and spray.
e. Set light timer with 6500k bulb on 12/12 schedule.
f. Turn thermostat down to 75 degrees F.
g. Fan three times a day. Do not spray.

12. Harvest and Dry
a. Harvest in a week or less.
b. Place in desiccant drying chamber and dry.
c. Place dry mushroom into double zip lock bags and suck air out.
d. Place in CO2 container with Schrader valve.
e. Charge container with CO2 with CO2 bike pump dispenser for long term storage.

13. Trip
a. Measure out 0.5g of dried mushroom. More if you know what you’re doing.
b. Eat mushrooms.
c. Put Bluetech on iPod.
d. Lay down.
e. Close Eyes.
f. Trip.
g. Return wiser and more informed.
h. Write down what you learned
i. Begin integration process.


--------------------
...because life is fleeting.

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OfflineCamtaro420
Birdman
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Registered: 11/25/13
Posts: 707
Loc: New Mexico
Last seen: 6 years, 5 months
Re: Grow Plan and Log for Panaeolus Cyanescens [Re: thatmonk]
    #19508847 - 02/02/14 12:15 AM (10 years, 11 months ago)

I haven't finished it yet but.

4. Prepare SAB
a. Wipe down interior and gloves with isopropyl alcohol and dry with paper towel.
c. Place glove box on work surface in laundry room upside down on a wet towel that's dampened with 10%bleach/90%water.
d. Set up glove box lighting.
e. Leave the lid off since it's upside down on a towel. let sit for twenty five minutes.
g. Wipe down petri dishes with alcohol and place in glove box.
h. Shake well the spore syringe.
i. Wipe down spore syringes with alcohol and place in glove box.
j. Wipe down scissors with alcohol and place in glove box.
k. Place Parafilm in glove box.
l. Set up alcohol burner next to glove box tool sterilization port and light after alcohol is dry.


Fixed this part for you. All that lysol sprayins is not needed and will kick up contams. The stiller the better. Clean with lysol the day before at least 24 hours


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Edited by Camtaro420 (02/02/14 12:15 AM)

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OfflineCamtaro420
Birdman
Male


Registered: 11/25/13
Posts: 707
Loc: New Mexico
Last seen: 6 years, 5 months
Re: Grow Plan and Log for Panaeolus Cyanescens [Re: thatmonk]
    #19508867 - 02/02/14 12:23 AM (10 years, 11 months ago)

5. Inoculate Petri Dishes
a. Shake spore syringe again.
b. Remove cap, attach needle.
c. Flame sterilize needle.
d. Place one-three drop of spore solution in each dish, in the middle, away from the edges.
e. Apply Parafilm seal on edge of Petri dish.
f. Place in incubator at 75 degrees

7. Inoculate WBS Spawn
a. Write inoculation date and mushroom species and race on jar sticker. Make notes in grow log.
b. Prepare self as in step 3.
c. Prepare SAB as in step 4.
d. Pick best looking culture.
e. Prep colonized Petri dish, and a blade that can be flame sterilized.
f. Prep alcohol burner near tool sterilizing port and light.
g. take a small slice of colonized growth and to add to the WBS
j. Remove foil lid.
k. Add one slice of agar into each WBS 1/2p spawn jars opening the, and closing them inside a SAB.
l. Remove from SAB and place in incubator at 75 degrees F.

Here's some more. Bed time for me.


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Offlinethatmonk
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Registered: 09/27/13
Posts: 160
Loc: Near Astoria, OR
Last seen: 8 years, 2 months
Re: Grow Plan and Log for Panaeolus Cyanescens [Re: Camtaro420]
    #19508891 - 02/02/14 12:31 AM (10 years, 11 months ago)

Shweet! This is gonna be fun! Next I'll write my grow plan for the Psilocybe Cyanescens bed going in outside.


--------------------
...because life is fleeting.

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OfflineCamtaro420
Birdman
Male


Registered: 11/25/13
Posts: 707
Loc: New Mexico
Last seen: 6 years, 5 months
Re: Grow Plan and Log for Panaeolus Cyanescens [Re: thatmonk]
    #19508938 - 02/02/14 12:49 AM (10 years, 11 months ago)

Quote:

thatmonk said:
Shweet! This is gonna be fun! Next I'll write my grow plan for the Psilocybe Cyanescens bed going in outside.




Keep up the good work. A lot more ppl will chime in to help it's just late.

Trust me there are a lot of steps u could cut out for effeciency, safety and simplicity.

Look up Frank Horrigan. http://www.shroomery.org/forums/showprofile.php?User=242121 All his write ups are pro and trusted, dude is boss.

cronicr will pop in to help any moment to say something too.


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OfflinecronicrMFacebook
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Registered: 08/07/11
Posts: 62,905
Loc: Van Isle Flag
Last seen: 27 minutes, 30 seconds
Trusted Cultivator
OG Cultivator
Re: Grow Plan and Log for Panaeolus Cyanescens [Re: Camtaro420]
    #19509157 - 02/02/14 02:32 AM (10 years, 11 months ago)

i'd suggest you try a sfd filter on the jars with no dry verm and and injection port, then you can do a 360 degree inoculation for fast colonization, i love doing this:rockon:


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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Offlinenksfo5
traverse traveler


Registered: 12/19/12
Posts: 689
Last seen: 10 months, 23 days
Re: Grow Plan and Log for Panaeolus Cyanescens [Re: cronicr]
    #19509366 - 02/02/14 04:38 AM (10 years, 11 months ago)

:nothingtoadd:

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