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Joust
Mycotographer




Registered: 10/13/11
Posts: 13,392
Loc: WA
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Re: Psilocybe silvatica [Re: Byrain]
#19508570 - 02/01/14 10:58 PM (10 years, 23 days ago) |
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Quote:
Byrain said: You should try to get some 1000x cystidia pics in a crush mount. Start with nothing but tiny!!!!! piece of the gill face and then smash it! You can then repeat that with the gill edge.
my sections were well enough to crush with my fine metal tip. I observed tons of cheilo, but no real pleuro, besides those little guys, i even did what i used to do (the method you stated above) to make sure i was isolating everything and i just got these guys as pleuro

see the cell there in the middle
-------------------- ~~~~~~***Psilocybin Mushrooms***~~~~~~ _________A Practical Guide To Psilocybin Mushrooms_________ "Think about the species, not your scale". -NeoSporen "Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira
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Joust
Mycotographer




Registered: 10/13/11
Posts: 13,392
Loc: WA
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Re: Psilocybe silvatica [Re: Byrain]
#19508580 - 02/01/14 11:00 PM (10 years, 23 days ago) |
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Quote:
Byrain said: You should try to get some 1000x cystidia pics in a crush mount. Start with nothing but tiny!!!!! piece of the gill face and then smash it! You can then repeat that with the gill edge.
i can either get 800x or 2000x, the ones above are 800x i can look at 1000x but i cant take micrographs cuz my adapter is at 20x not 10
-------------------- ~~~~~~***Psilocybin Mushrooms***~~~~~~ _________A Practical Guide To Psilocybin Mushrooms_________ "Think about the species, not your scale". -NeoSporen "Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira
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Byrain

Registered: 01/07/10
Posts: 9,664
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Re: Psilocybe silvatica [Re: Joust]
#19508601 - 02/01/14 11:03 PM (10 years, 23 days ago) |
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Get out your point & shoot and aim it down the eyepiece?
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Joust
Mycotographer




Registered: 10/13/11
Posts: 13,392
Loc: WA
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Re: Psilocybe silvatica [Re: Byrain]
#19508615 - 02/01/14 11:05 PM (10 years, 23 days ago) |
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Quote:
Byrain said: Get out your point & shoot and aim it down the eyepiece?
cant do measurements that way though. im not sure what you are wanting, cystidia measurements or are you wanting to see cheilo and pleruo morphology, cheilo is easy to capture, but i spend 2 hours looking for pleuro and only found those little guys.
Thanks for the input Byrain, just trying to work something out here. Soon ill be able to use the nice university scopes!~!
-------------------- ~~~~~~***Psilocybin Mushrooms***~~~~~~ _________A Practical Guide To Psilocybin Mushrooms_________ "Think about the species, not your scale". -NeoSporen "Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira
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Joust
Mycotographer




Registered: 10/13/11
Posts: 13,392
Loc: WA
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Re: Psilocybe silvatica [Re: Joust]
#19508638 - 02/01/14 11:10 PM (10 years, 23 days ago) |
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Ill do my best to get some micrographs out of cytidia
-------------------- ~~~~~~***Psilocybin Mushrooms***~~~~~~ _________A Practical Guide To Psilocybin Mushrooms_________ "Think about the species, not your scale". -NeoSporen "Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira
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Byrain

Registered: 01/07/10
Posts: 9,664
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Re: Psilocybe silvatica [Re: Joust]
#19508677 - 02/01/14 11:19 PM (10 years, 23 days ago) |
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Both would be nice, but I was thinking morphology. Look for more of these cells, are they not pleurocystidia?
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Joust
Mycotographer




Registered: 10/13/11
Posts: 13,392
Loc: WA
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Re: Psilocybe silvatica [Re: Byrain]
#19508692 - 02/01/14 11:23 PM (10 years, 23 days ago) |
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Quote:
Byrain said: Both would be nice, but I was thinking morphology. Look for more of these cells, are they not pleurocystidia?

those are all on the gill edge, those are all Cheilocystidia, thats the sterile band.
-------------------- ~~~~~~***Psilocybin Mushrooms***~~~~~~ _________A Practical Guide To Psilocybin Mushrooms_________ "Think about the species, not your scale". -NeoSporen "Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira
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Byrain

Registered: 01/07/10
Posts: 9,664
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Re: Psilocybe silvatica [Re: Joust]
#19508699 - 02/01/14 11:24 PM (10 years, 23 days ago) |
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Ah shit, I thought that was labeled pleurocystidia, I should go to bed...
Yea, your pleurocystidia images look like basidioles.
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Joust
Mycotographer




Registered: 10/13/11
Posts: 13,392
Loc: WA
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Re: Psilocybe silvatica [Re: Byrain]
#19508718 - 02/01/14 11:28 PM (10 years, 23 days ago) |
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Quote:
Byrain said: Ah shit, I thought that was labeled pleurocystidia, I should go to bed...
Yea, your pleurocystidia images look like basidioles.
thats what i thought tooo. check this out!
heres the 2000x CHEILOcystidia  



-------------------- ~~~~~~***Psilocybin Mushrooms***~~~~~~ _________A Practical Guide To Psilocybin Mushrooms_________ "Think about the species, not your scale". -NeoSporen "Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira
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Byrain

Registered: 01/07/10
Posts: 9,664
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Re: Psilocybe silvatica [Re: Joust]
#19508722 - 02/01/14 11:30 PM (10 years, 23 days ago) |
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Nice.
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Joust
Mycotographer




Registered: 10/13/11
Posts: 13,392
Loc: WA
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Re: Psilocybe silvatica [Re: Byrain]
#19508739 - 02/01/14 11:38 PM (10 years, 23 days ago) |
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Quote:
Byrain said: Nice. 
some of them look like thumb ups haha
They sure do look like psilocybe cheilo to my eye. Alonso is going to be helpful on this one, i asked him about it.
-------------------- ~~~~~~***Psilocybin Mushrooms***~~~~~~ _________A Practical Guide To Psilocybin Mushrooms_________ "Think about the species, not your scale". -NeoSporen "Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira
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nomadbrad
Oregrownian



Registered: 10/23/06
Posts: 1,160
Loc: Pacific NW
Last seen: 5 years, 4 months
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Re: Psilocybe silvatica [Re: Joust] 1
#19508784 - 02/01/14 11:57 PM (10 years, 23 days ago) |
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This is awesome news. Although I just toured 5 micro breweries this evening and tried 40+ beers I'm still stoked and coherent enough to understand the ramifications of not finding chrysostidia!
Joust thanks for not just dicking this specimen off as Hypholoma! Haha love you dude
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Joust
Mycotographer




Registered: 10/13/11
Posts: 13,392
Loc: WA
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Re: Psilocybe silvatica [Re: nomadbrad]
#19508827 - 02/02/14 12:08 AM (10 years, 23 days ago) |
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Quote:
nomadbrad said:
 
This is awesome news. Although I just toured 5 micro breweries this evening and tried 40+ beers I'm still stoked and coherent enough to understand the ramifications of not finding chrysostidia!
Joust thanks for not just dicking this specimen off as Hypholoma! Haha love you dude 
Oh i wasnt about to, that was my initial thought, i knew i had more work to do, but one can only micro for so long, it takes a few micro sessions, usually easch session i try and change parameters of veiwing. I typically use Congo red stain or Phyloxen or whatever its called, I toured... my personal herbariaum of palnts tonight. so im less coherant than you! woop. spellign, bet you guys didnt know im dyslexic, and im pretty sure it gets worse as i smoked pot. So yeah. now i just suck at math and backwards things and wirte wierd sometimes. But i digress....
This last time i used KOH and just used that,. Next time ill hydrate with some other stain and maybe use gsm again, mount differently for sure... but there will likely be a third micro if Alan has the time to chime in.
-------------------- ~~~~~~***Psilocybin Mushrooms***~~~~~~ _________A Practical Guide To Psilocybin Mushrooms_________ "Think about the species, not your scale". -NeoSporen "Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira
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Alan Rockefeller
Mycologist

Registered: 03/10/07
Posts: 48,312
Last seen: 3 days, 23 hours
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Quote:
psylosymonreturns said: Alan Where are you?
I just got back from a party in Berkeley. I gave my Psilocybe talk earlier in the day in exchange for free admission, and later in the night I brought a microscope. They set me up in a party bus next to a fortune teller lady. So I got to hear all the peoples fortunes as I scoped spores from Mexico. She was a really nice fortune teller and told everyone good things about their future. Here is a pic from 30 minutes ago, after the fortune teller left...

I studied the type collection of Psilocybe silvatica two months ago and will look for my notes.
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psylosymonreturns
aka Gym Sporrison



Registered: 10/16/09
Posts: 13,948
Loc: Mos Eisley,
Last seen: 3 years, 6 months
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Yes you did didn't you! This could get interesting!
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Joust
Mycotographer




Registered: 10/13/11
Posts: 13,392
Loc: WA
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Nice scope!
-------------------- ~~~~~~***Psilocybin Mushrooms***~~~~~~ _________A Practical Guide To Psilocybin Mushrooms_________ "Think about the species, not your scale". -NeoSporen "Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira
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Lepkaun
Super Stooper



Registered: 10/07/10
Posts: 2,093
Loc: Close and Far
Last seen: 8 years, 4 months
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Re: Psilocybe silvatica [Re: Joust]
#19510772 - 02/02/14 12:13 PM (10 years, 22 days ago) |
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Cool scope Alan!
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Leppy say's: Seek and you will find!Ask and they will guide you!Listen and be taught!!
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psylosymonreturns
aka Gym Sporrison



Registered: 10/16/09
Posts: 13,948
Loc: Mos Eisley,
Last seen: 3 years, 6 months
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Re: Psilocybe silvatica [Re: Lepkaun]
#19511969 - 02/02/14 04:28 PM (10 years, 22 days ago) |
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But Joust, those pics are stellar bud!! Nice job!
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maynardjameskeenan
The white stipes



Registered: 11/11/10
Posts: 16,391
Loc: 'Merica
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Quote:
psylosymonreturns said: But Joust, those pics are stellar bud!! Nice job!
 These mircographs look amazing, far better than I expected I'm sorry for being a dick about you not posting any a few days ago. I can get a little irritable at times, I'm working on it.
Are you taking the pictures with you DSLR and then Photoshopping them? The resolution looks huge compared to the ones that goes in my eye piece.
What did you use to rehydrate the mushrooms in the last set of pictures, is it just water? Is that what a crush mount is supposed to look like?
-------------------- May you be filled with loving kindness. May you be well. May you be peaceful and at ease. May you be happy. AMU Q&A
Edited by maynardjameskeenan (02/02/14 04:47 PM)
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Joust
Mycotographer




Registered: 10/13/11
Posts: 13,392
Loc: WA
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Quote:
maynardjameskeenan said:
Quote:
psylosymonreturns said: But Joust, those pics are stellar bud!! Nice job!
 These mircographs look amazing, far better than I expected I'm sorry for being a dick about you not posting any a few days ago. I can get a little irritable at times, I'm working on it.
Are you taking the pictures with you DSLR and then Photoshopping them? The resolution looks huge compared to the ones that goes in my eye piece.
What did you use to rehydrate the mushrooms in the last set of pictures, is it just water? Is that what a crush mount is supposed to look like?
I will take a video of how i do things... its way to hard to show and i have a few tricks ive developed thanks to hearing what others do....
-------------------- ~~~~~~***Psilocybin Mushrooms***~~~~~~ _________A Practical Guide To Psilocybin Mushrooms_________ "Think about the species, not your scale". -NeoSporen "Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira
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