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Offlinesytar
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Some old agar of mine is fruiting/pinning
    #19507271 - 02/01/14 05:29 PM (10 years, 11 months ago)

So I inoculated some agar with MS around late November, close to early December. They've just been sitting in a kitchen cabinet all this time. I have nine plates which have what honestly look like fruits. One of them is a good four inches long and jumped out of its section and across another. I'm wondering if it is worth my time to transfer these fruits to another plate of agar then inoculate some grain with them.

I'm worried first of all about senescence. Two months is a while. If they produce fruits might those fruits be too old to be the stuff of a master slant?

My second worry is about what kind of genetics I would be selecting for. Obviously these bastards can withstand incredibly hostile conditions and love to fruit. If they grew so much in agar, does that really say how they would grow in grains?



Looking forward to hearing your opinions!

:mushroom2: :peace:


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OfflineAero
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Re: Some old agar of mine is fruiting/pinning [Re: sytar]
    #19507275 - 02/01/14 05:32 PM (10 years, 11 months ago)

pic or it didnt happen :evil:


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Re: Some old agar of mine is fruiting/pinning [Re: Aero]
    #19507300 - 02/01/14 05:36 PM (10 years, 11 months ago)

Pins on plates are great to clone as soon as you first see them. Usually a good aggressive fruiting strain but, you want to clone when they are still pins as that's when genetics are fast and aggressive. Most of my best cultures were cloned from pins off of agar.

I wouldn't even bother with something this far along.


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Re: Some old agar of mine is fruiting/pinning [Re: Aero]
    #19507307 - 02/01/14 05:38 PM (10 years, 11 months ago)

Bit hard to see due to condensation but I took pics of two of them.



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Re: Some old agar of mine is fruiting/pinning [Re: sytar]
    #19507330 - 02/01/14 05:44 PM (10 years, 11 months ago)

I would transfer those soon.

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Re: Some old agar of mine is fruiting/pinning [Re: sytar]
    #19507686 - 02/01/14 07:08 PM (10 years, 11 months ago)

Quote:

sytar said:


I'm worried first of all about senescence. Two months is a while. If they produce fruits might those fruits be too old to be the stuff of a master slant?





senscene is something that happens with the number of cell divisions not time, but as pasty said if there not mature transfer them


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Re: Some old agar of mine is fruiting/pinning [Re: cronicr]
    #19507712 - 02/01/14 07:14 PM (10 years, 11 months ago)

I thought I remember RR saying that senescence happens both in terms of expansion/cell divisions and chronological time. And maybe something else about chronological time being counted slower when the temperature is lower.

Could be wrong, I'm not an expert, but I feel pretty sure that I heard this from him because it surprised me.

In any case, I'm sure you guys are right about transferring these. I put them in the fridge for now, will transfer when my tools get here.

Edit
Here is a quote I dug up, though I think I got the info from let's grow mushrooms.

Quote:


The number of cell divisions determines to a large extent the amount of senescence.  Chronological time also plays a role.  Nobody allows mycelium to completely crawl over the surface of a petri dish, or at least they shouldn't.

You don't grow to fruiting and then clone.  Once you have a culture, you simply go back to those young cell lines for the next crop.  If you're careful, senescence will never set in.  Some of my very best commercial strains have been my possession over 30 years.
RR


http://www.shroomery.org/forums/showflat.php/Number/17893040




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Edited by sytar (02/01/14 07:17 PM)

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Re: Some old agar of mine is fruiting/pinning [Re: sytar]
    #19507725 - 02/01/14 07:16 PM (10 years, 11 months ago)

i'm sure a culture could die over time but not in the timeframe you have, maybe if it dried up


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Re: Some old agar of mine is fruiting/pinning [Re: cronicr]
    #19508268 - 02/01/14 09:46 PM (10 years, 11 months ago)

I ended up just using some tweezers I had lying around to do the transfers. Is there a better tool I should consider using?


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Re: Some old agar of mine is fruiting/pinning [Re: sytar]
    #19508299 - 02/01/14 09:57 PM (10 years, 11 months ago)

tweezers are fine


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