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Barnabey
Learner



Registered: 06/19/13
Posts: 249
Loc:
Last seen: 4 years, 7 months
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Spores germinating in syringe ?
#19505823 - 02/01/14 11:44 AM (10 years, 11 months ago) |
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Hello everyone !
So my friend successfully grew enokis outdoor from dowels he ordered. When visiting him, I took the best looking fresh enoki and made a spore print. Back in my country, I attempted to make a spore syringe, using a sterelized syringe, distilled water that went in the PC... did everything in glove box etc...
1. The enoki had some moisture before I foiled the aluminium back then so the aluminium foil was like stuck together when trying to open my print. I know the humidity in my spore print would encourage bacterial contamination, anyway, this is what i had to add !
Now looking at my syringe the is sitting in the fridge, it looks like I have something growing in it ! (i also have small chunks of tiny white thing that I assume are tiny pieces of mycelium, but this, stuck in the corner ? Can it be really enoki mycelium or something else ? I'm sorry I don't have a macro or a way to make that pic clearer :O
-------------------- My cute contaminants
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cronicr



Registered: 08/07/11
Posts: 62,905
Loc: Van Isle
Last seen: 4 minutes, 4 seconds
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Re: Spores germinating in syringe ? [Re: Barnabey]
#19505850 - 02/01/14 11:53 AM (10 years, 11 months ago) |
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it very well could be something else, i would put it to agar first
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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Barnabey
Learner



Registered: 06/19/13
Posts: 249
Loc:
Last seen: 4 years, 7 months
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Re: Spores germinating in syringe ? [Re: cronicr]
#19505878 - 02/01/14 12:03 PM (10 years, 11 months ago) |
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I only inoculated two plates of agar and I'm about to check them out in the glove box because I'm very confused about what's going on without opening it ! One shows what looks like mycelium and the other one doesn't show anything and looks like a layer of something covered the entire plate but it's hard to tell until I opened it (stupid condensation + parafilm covering too much of the plate )
I will make more agar plates with it and post pics in here.
Anyone has experience with a spore print in aluminium foil that was sticking together (not wet, it was defenitly dry when I opened it but it was probably a little too humid at the time i folded it) ?
-------------------- My cute contaminants
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cronicr



Registered: 08/07/11
Posts: 62,905
Loc: Van Isle
Last seen: 4 minutes, 4 seconds
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Re: Spores germinating in syringe ? [Re: Barnabey]
#19505898 - 02/01/14 12:07 PM (10 years, 11 months ago) |
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yeah it's always good practice to let your print dry before storing it
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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Aero
Orea


Registered: 11/01/13
Posts: 2,253
Last seen: 2 years, 2 months
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Re: Spores germinating in syringe ? [Re: cronicr]
#19505974 - 02/01/14 12:28 PM (10 years, 11 months ago) |
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i often get germination in the spore syringes, specially when i store them at room temp
-------------------- SPREAD THE SPORES
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cronicr



Registered: 08/07/11
Posts: 62,905
Loc: Van Isle
Last seen: 4 minutes, 4 seconds
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Re: Spores germinating in syringe ? [Re: Aero]
#19505978 - 02/01/14 12:30 PM (10 years, 11 months ago) |
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i rarely get it with distilled water though, but if the print wasn't dried first bacteria and other thing may have already germinated
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
|
Aero
Orea


Registered: 11/01/13
Posts: 2,253
Last seen: 2 years, 2 months
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Re: Spores germinating in syringe ? [Re: cronicr]
#19506036 - 02/01/14 12:46 PM (10 years, 11 months ago) |
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yea i use mineral water
-------------------- SPREAD THE SPORES
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PirateSwazey



Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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Re: Spores germinating in syringe ? [Re: cronicr]
#19506252 - 02/01/14 01:34 PM (10 years, 11 months ago) |
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Quote:
cronicr said: it very well could be something else, i would put it to agar first
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Barnabey
Learner



Registered: 06/19/13
Posts: 249
Loc:
Last seen: 4 years, 7 months
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Re: Spores germinating in syringe ? [Re: PirateSwazey]
#19506281 - 02/01/14 01:44 PM (10 years, 11 months ago) |
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Yes, I was of course going to use this in agar no matter what :p
-------------------- My cute contaminants
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