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OfflineBarnabey
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Spores germinating in syringe ?
    #19505823 - 02/01/14 11:44 AM (10 years, 11 months ago)

Hello everyone !

So my friend successfully grew enokis outdoor from dowels he ordered.
When visiting him, I took the best looking fresh enoki and made a spore print.
Back in my country, I attempted to make a spore syringe, using a sterelized syringe, distilled water that went in the PC... did everything in glove box etc...

1. The enoki had some moisture before I foiled the aluminium back then so the aluminium foil was like stuck together when trying to open my print. I know the humidity in my spore print would encourage bacterial contamination, anyway, this is what i had to add !

Now looking at my syringe the is sitting in the fridge, it looks like I have something growing in it ! (i also have small chunks of tiny white thing that I assume are tiny pieces of mycelium, but this, stuck in the corner ? Can it be really enoki mycelium or something else ? I'm sorry I don't have a macro or a way to make that pic clearer :O



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Re: Spores germinating in syringe ? [Re: Barnabey]
    #19505850 - 02/01/14 11:53 AM (10 years, 11 months ago)

it very well could be something else, i would put it to agar first


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OfflineBarnabey
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Re: Spores germinating in syringe ? [Re: cronicr]
    #19505878 - 02/01/14 12:03 PM (10 years, 11 months ago)

I only inoculated two plates of agar and I'm about to check them out in the glove box because I'm very confused about what's going on without opening it ! One shows what looks like mycelium and the other one doesn't show anything and looks like a layer of something covered the entire plate but it's hard to tell until I opened it (stupid condensation + parafilm covering too much of the plate :laugh: )


I will make more agar plates with it and post pics in here.

Anyone has experience with a spore print in aluminium foil that was sticking together (not wet, it was defenitly dry when I opened it but it was probably a little too humid at the time i folded it) ?


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Re: Spores germinating in syringe ? [Re: Barnabey]
    #19505898 - 02/01/14 12:07 PM (10 years, 11 months ago)

yeah it's always good practice to let your print dry before storing it:cool:


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OfflineAero
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Re: Spores germinating in syringe ? [Re: cronicr]
    #19505974 - 02/01/14 12:28 PM (10 years, 11 months ago)

i often get germination in the spore syringes,
specially when i store them at room temp


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Re: Spores germinating in syringe ? [Re: Aero]
    #19505978 - 02/01/14 12:30 PM (10 years, 11 months ago)

i rarely get it with distilled water though, but if the print wasn't dried first bacteria and other thing may have already germinated


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OfflineAero
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Re: Spores germinating in syringe ? [Re: cronicr]
    #19506036 - 02/01/14 12:46 PM (10 years, 11 months ago)

yea i use mineral water


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Re: Spores germinating in syringe ? [Re: cronicr]
    #19506252 - 02/01/14 01:34 PM (10 years, 11 months ago)

Quote:

cronicr said:
it very well could be something else, i would put it to agar first



:whathesaid:


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OfflineBarnabey
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Re: Spores germinating in syringe ? [Re: PirateSwazey]
    #19506281 - 02/01/14 01:44 PM (10 years, 11 months ago)

Yes, I was of course going to use this in agar no matter what :p


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