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sillehcybeh
Stranger


Registered: 01/04/14
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Two weeks after fruiting this cake, here's what I have to show for it.
#19452342 - 01/21/14 11:21 AM (10 years, 11 months ago) |
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To be fair though, I kinda think I 'dun goofed' and got my measurements wrong. Basically I now realize I probably put far too little BRF during sub prep plus too much water. Otherwise I got to 100%, consolidated 7 days and dunked 24h before birthing. I fan, mist and house my project on a SGFG that is raised @20c. So yeah below is just about it. Rest of the cake has just a bunch of small aborts.

Sadly I'm bang out of spores. Oh how I would die for a print which that cake was supposed to give me 
BUT I have two sterile jars currently colonizing. Cuz I'm a bit broke, do you reckon under a SAB I could do a mycellium tissue transfer and drop it into a newly PC'ed PFTEK cake (with verm/brf measured properly) instead of buying a new syringe for spores?
Edited by sillehcybeh (01/21/14 11:23 AM)
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magickspore



Registered: 12/11/12
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Re: Two weeks after fruiting this cake, here's what I have to show for it. [Re: sillehcybeh]
#19452360 - 01/21/14 11:25 AM (10 years, 11 months ago) |
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You have two choices the second being the best option. 1st yes you could but I'd drop the mycelium on agar. The 2nd pluck one of those pins and drop that on agar.
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Gabe_18
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Registered: 12/29/13
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Re: Two weeks after fruiting this cake, here's what I have to show for it. [Re: sillehcybeh]
#19452365 - 01/21/14 11:26 AM (10 years, 11 months ago) |
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I've heard of people transferring mycelium tissue to rye seed. They had success. I wouldnt see why it wouldn't work for the pf tek. Also if you really need some spores, I can help you out, just pm me about it if interested.
-------------------- Mathematics is the language in which God has written the universe- Galileo Galilei
I really love plants and fungi!!!!!
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cronicr



Registered: 08/07/11
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Loc: Van Isle
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Re: Two weeks after fruiting this cake, here's what I have to show for it. [Re: magickspore]
#19452385 - 01/21/14 11:31 AM (10 years, 11 months ago) |
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Quote:
Gabe_18 said: I've heard of people transferring mycelium tissue to rye seed. They had success. I wouldnt see why it wouldn't work for the pf tek. Also if you really need some spores, I can help you out, just pm me about it if interested.
well for one it's already in fruiting and covered in contaminates
Quote:
magickspore said: You have two choices the second being the best option. 1st yes you could but I'd drop the mycelium on agar. The 2nd pluck one of those pins and drop that on agar.
option 3, both lol but i would go with 2 as well
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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Happy Littletree
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Registered: 01/02/14
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Re: Two weeks after fruiting this cake, here's what I have to show for it. [Re: sillehcybeh]
#19452438 - 01/21/14 11:42 AM (10 years, 11 months ago) |
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I would think it would be best if you cut the abort open in you SAB and remove some clean flesh for whatever you try but I don't see a way to do a pf with verm and not contaminate it or be at super high risk of contamination.
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Re: Two weeks after fruiting this cake, here's what I have to show for it. [Re: Happy Littletree]
#19452449 - 01/21/14 11:44 AM (10 years, 11 months ago) |
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Quote:
Happy Littletree said: I would think it would be best if you cut the abort open in you SAB and remove some clean flesh for whatever you try but I don't see a way to do a pf with verm and not contaminate it or be at super high risk of contamination.
Don't cut tissue samples the blade drags contaminantion on the outside of the sample into the core. Tear tissue samples in half to expose clean tissue. You can't really tear a pin but they're usually so vigorous they bury contamination and grow just fine(on 2D media Don't try it in sterile grains) but take a transfer or two to be safe.
Edited by Trusted cuItivator (01/21/14 11:45 AM)
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Happy Littletree
One

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Re: Two weeks after fruiting this cake, here's what I have to show for it. [Re: bodhisatta]
#19452467 - 01/21/14 11:47 AM (10 years, 11 months ago) |
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Thanks for clarifying
Edited by Happy Littletree (01/21/14 11:48 AM)
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cronicr



Registered: 08/07/11
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Re: Two weeks after fruiting this cake, here's what I have to show for it. [Re: bodhisatta]
#19452663 - 01/21/14 12:28 PM (10 years, 11 months ago) |
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don't cut fruits tear them, also don't clone abortsQuote:
bodhisatta said:
Quote:
Happy Littletree said: I would think it would be best if you cut the abort open in you SAB and remove some clean flesh for whatever you try but I don't see a way to do a pf with verm and not contaminate it or be at super high risk of contamination.
Don't cut tissue samples the blade drags contaminantion on the outside of the sample into the core. Tear tissue samples in half to expose clean tissue. You can't really tear a pin but they're usually so vigorous they bury contamination and grow just fine(on 2D media Don't try it in sterile grains) but take a transfer or two to be safe.
also never clone an abort lol
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Re: Two weeks after fruiting this cake, here's what I have to show for it. [Re: cronicr]
#19452679 - 01/21/14 12:32 PM (10 years, 11 months ago) |
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I said pin rather than reiterate abort in my reply but good catch I didn't point it out.
Don't clone an abort. You want to possibly carry on a linage of failure?
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sillehcybeh
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Registered: 01/04/14
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Re: Two weeks after fruiting this cake, here's what I have to show for it. [Re: sillehcybeh]
#19452698 - 01/21/14 12:37 PM (10 years, 11 months ago) |
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Hey good tips, good tips thanks a lot also for the assistance on obtaining more spores...
Back to the cake, do you think it's done. Shall I just toss it and cut my losses?
There's another cake in the SGFG showing exactly the same characteristics as the cake in question, ultra slow pinning even though I consolidated that one for an extra week and a bit. So the only explanation I can conclude is not enough BRF will lead to this shyte. Getting trashy genetics twice in a row on B+ seems unlikely.
Oh and above me now there is a cake that is colonizing, forget the abort cake in the SGFG I was going to use the jar to extract the mycellium when it was done under a SAB as it should be sterile enough..for tissue for a transfer to a new sub. I'd go for bird seed but I still have loads of verm and a bit more BRF so I'll just finish that off first before giving different teks a go
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Edited by sillehcybeh (01/21/14 12:39 PM)
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Re: Two weeks after fruiting this cake, here's what I have to show for it. [Re: sillehcybeh]
#19452713 - 01/21/14 12:40 PM (10 years, 11 months ago) |
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The cakes are probably fine. You have enough BRF. You could cut the BRF in half and still get shrooms just fine. I have a feeling your SGFC isnt built right. Take a picture of the SGFC.
Edited by Trusted cuItivator (01/21/14 12:40 PM)
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sillehcybeh
Stranger


Registered: 01/04/14
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Re: Two weeks after fruiting this cake, here's what I have to show for it. [Re: bodhisatta]
#19452764 - 01/21/14 12:53 PM (10 years, 11 months ago) |
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Ok here we go...
Bottom

End

Side

Top

Check out OK?
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Re: Two weeks after fruiting this cake, here's what I have to show for it. [Re: sillehcybeh]
#19452772 - 01/21/14 12:54 PM (10 years, 11 months ago) |
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should be alright. More elevation would be better I like to have at leaast 6 inches on all sides including the bottom. You're misting them every time they dry out?
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Quexl

Registered: 12/17/13
Posts: 1,443
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Last seen: 9 years, 10 months
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Re: Two weeks after fruiting this cake, here's what I have to show for it. [Re: bodhisatta]
#19452794 - 01/21/14 01:00 PM (10 years, 11 months ago) |
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You may want to consider room conditions too (temp/humidity/location of FC). 
..Selectively grumpy fruiting chamber. *hide*
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sillehcybeh
Stranger


Registered: 01/04/14
Posts: 136
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Re: Two weeks after fruiting this cake, here's what I have to show for it. [Re: bodhisatta]
#19452809 - 01/21/14 01:04 PM (10 years, 11 months ago) |
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Ok awesome I'll see if I can find something to raise it a bit higher. Yeah, I'm misting about 2-3 times a day lightly and fanning it till it sparkles instead of letting it be soggy.
I even injected 10cc of tap water into that cake distributed in various areas. Maybe eBay spores just suck, but then again how the feck would he/she have fruited something as crappy as this lol
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vikingsc
Mushroom Enthusiast



Registered: 01/11/12
Posts: 1,214
Loc: British Columbia, Canada
Last seen: 11 days, 14 hours
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Re: Two weeks after fruiting this cake, here's what I have to show for it. [Re: sillehcybeh]
#19453401 - 01/21/14 03:29 PM (10 years, 11 months ago) |
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Quote:
sillehcybeh said: To be fair though, I kinda think I 'dun goofed' and got my measurements wrong. Basically I now realize I probably put far too little BRF during sub prep plus too much water. Otherwise I got to 100%, consolidated 7 days and dunked 24h before birthing. I fan, mist and house my project on a SGFG that is raised @20c. So yeah below is just about it. Rest of the cake has just a bunch of small aborts.

Sadly I'm bang out of spores. Oh how I would die for a print which that cake was supposed to give me 
BUT I have two sterile jars currently colonizing. Cuz I'm a bit broke, do you reckon under a SAB I could do a mycellium tissue transfer and drop it into a newly PC'ed PFTEK cake (with verm/brf measured properly) instead of buying a new syringe for spores?
You dunked 24hr before birthing? So you poured water in the jar? You would usually birth before you dunk.
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sillehcybeh
Stranger


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Re: Two weeks after fruiting this cake, here's what I have to show for it. [Re: vikingsc]
#19453507 - 01/21/14 03:56 PM (10 years, 11 months ago) |
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Did exactly what Marc does on his website, removed from jar, rinsed off loose verm and placed in a pan with a plate to keep the cake submerged, left for 24h, rinsed, rolled it around in some verm 
TBH, I just dunno why my grow is sucking. I think the picture is a mutant anyway. I probably have enough pins and aborts for a decent microdose trip. Not sure I'm ready to get totalled and fucked again though, I took down 30 dried liberty caps and got spent. Lightweight or what :P
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sillehcybeh
Stranger


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Loc: United States of England
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Re: Two weeks after fruiting this cake, here's what I have to show for it. [Re: sillehcybeh]
#19502086 - 01/31/14 04:18 PM (10 years, 11 months ago) |
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So I just went away for 5 days. And here's what greets me when I return....
HOLY FUCK MOMENT.



Unfortunately, one cake got spore/blasted. I tried to clean it up as best as I could under the tap before redunking for another flush. However is it too late? I saw somewhere that when spores make contact with mycelium it tells the cake to shut down, or something. Lifecycle complete?
Anyone?
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cronicr



Registered: 08/07/11
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Re: Two weeks after fruiting this cake, here's what I have to show for it. [Re: sillehcybeh]
#19502092 - 01/31/14 04:20 PM (10 years, 11 months ago) |
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may or may not, just toss it back in it will probably put more out
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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vikingsc
Mushroom Enthusiast



Registered: 01/11/12
Posts: 1,214
Loc: British Columbia, Canada
Last seen: 11 days, 14 hours
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Re: Two weeks after fruiting this cake, here's what I have to show for it. [Re: cronicr]
#19502327 - 01/31/14 05:15 PM (10 years, 11 months ago) |
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Nice greeting to come home to. Definitely nicer than the earlier pics. That one cake was ready to be harvested at least a couple days ago.
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