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udntsay
Enthusiast ;)


Registered: 07/26/13
Posts: 642
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can i re inoculate?
#19500754 - 01/31/14 11:05 AM (10 years, 11 months ago) |
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Its been over a week or so. Can I re noc up the jars?
-------------------- ~ For educational purposes ~
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,870
Last seen: 2 months, 8 days
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Re: can i re inoculate? [Re: udntsay]
#19500796 - 01/31/14 11:13 AM (10 years, 11 months ago) |
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It wouldn't help anything. You'll probably see some growth soon though unless they're contaminated.
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Re: can i re inoculate? [Re: Kizzle]
#19500972 - 01/31/14 11:55 AM (10 years, 11 months ago) |
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wait until it's been 21 days if you don't see anything then start over. Re-inoculation rarely if ever works. You're just adding more water and more dirt since syringes are not quite 100% clean the more you use the more your chances are of seeing a contam.
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1down5up
Social Ninja



Registered: 11/04/13
Posts: 1,743
Last seen: 7 months, 2 days
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Re: can i re inoculate? [Re: bodhisatta]
#19500977 - 01/31/14 11:57 AM (10 years, 11 months ago) |
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If you're doing BRf cakes too, they tend to turn into glue after a while, they get really rubbery and compacted man. I don't think any mush myc could colonize them after too long...
-------------------- ~~Everything is relative~~
A Simplified Overview of Mushroom Cultivation Strategies - RR says - EvilMushroom666's Take on BRF Cakes - Frank's list of goodies - Cronicr's Goodies
No one is placed higher than another no matter race or creed or gender, we must teach forgiveness and compassion for all life. J.L.
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PsYcHoDoUgHbOy
Connoisseur



Registered: 08/11/08
Posts: 1,483
Last seen: 9 days, 14 hours
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Re: can i re inoculate? [Re: bodhisatta]
#19500981 - 01/31/14 12:00 PM (10 years, 11 months ago) |
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Quote:
bodhisatta said: wait until it's been 21 days if you don't see anything then start over. Re-inoculation rarely if ever works. You're just adding more water and more dirt since syringes are not quite 100% clean the more you use the more your chances are of seeing a contam.
What about attempting a grain to grain transfer with jars that did nothing? I have about 10 jars of rye that have not grown, but also show no sign of contamination. They are all over 30 days old.
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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I wouldn't personally bother trying, but if you have nothing to lose I guess you could. For me it would just be a setback, I could go PC a batch of new jars and be sure.
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Happy Littletree
One

Registered: 01/02/14
Posts: 1,386
Loc: slightly elevated from ea...
Last seen: 2 years, 2 months
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Did you get your spores from a sponsor vendor?
Edited by Happy Littletree (01/31/14 12:05 PM)
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PsYcHoDoUgHbOy
Connoisseur



Registered: 08/11/08
Posts: 1,483
Last seen: 9 days, 14 hours
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Quote:
Happy Littletree said: Did you get your spores from a sponsor vendor?
I know I'm not the OP, and I don't want to jack this thread... but yes I did. The PE and Hawaiian's have both done well, however the Burma have done nothing, and most of the PEUncut (besides 1) have done nothing. I am thinking about attempting a G2G on some of the jars with some Hawaiian colonized grain. I do have nothing to lose, if they contaminate they weren't going to grow anything anyway, and if they colonize yip-eee!
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Happy Littletree
One

Registered: 01/02/14
Posts: 1,386
Loc: slightly elevated from ea...
Last seen: 2 years, 2 months
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I would do like bodhisatta said.. I would throw the old stuff outside in a patch if possible. That way you may still get something out of it later. No one can tell you it won't work definitively but it's not recommended. If you do decide to do it make sure and smell the jars and if you smell anything weird toss it for sure!
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vikingsc
Mushroom Enthusiast



Registered: 01/11/12
Posts: 1,214
Loc: British Columbia, Canada
Last seen: 11 days, 14 hours
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Quote:
PsYcHoDoUgHbOy said:
Quote:
bodhisatta said: wait until it's been 21 days if you don't see anything then start over. Re-inoculation rarely if ever works. You're just adding more water and more dirt since syringes are not quite 100% clean the more you use the more your chances are of seeing a contam.
What about attempting a grain to grain transfer with jars that did nothing? I have about 10 jars of rye that have not grown, but also show no sign of contamination. They are all over 30 days old.
If you're gonna re-use old spawn jars I'd at least re-sterilize them first.
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udntsay
Enthusiast ;)


Registered: 07/26/13
Posts: 642
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Re: can i re inoculate? [Re: vikingsc]
#19501188 - 01/31/14 12:51 PM (10 years, 11 months ago) |
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There homemade ksss's + cambos. 2 of the ksss jars show little growth out of the 4 The 7cambo jars I have sent doing anything. I think I nocced em up the 21&22nd
-------------------- ~ For educational purposes ~
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Re: can i re inoculate? [Re: vikingsc]
#19501487 - 01/31/14 02:10 PM (10 years, 11 months ago) |
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Quote:
vikingsc said:
Quote:
PsYcHoDoUgHbOy said:
Quote:
bodhisatta said: wait until it's been 21 days if you don't see anything then start over. Re-inoculation rarely if ever works. You're just adding more water and more dirt since syringes are not quite 100% clean the more you use the more your chances are of seeing a contam.
What about attempting a grain to grain transfer with jars that did nothing? I have about 10 jars of rye that have not grown, but also show no sign of contamination. They are all over 30 days old.
If you're gonna re-use old spawn jars I'd at least re-sterilize them first.
that never works, the grains burn.
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cronicr



Registered: 08/07/11
Posts: 62,905
Loc: Van Isle
Last seen: 32 minutes, 12 seconds
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Re: can i re inoculate? [Re: bodhisatta]
#19501510 - 01/31/14 02:14 PM (10 years, 11 months ago) |
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grains are cheap, start over
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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