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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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long term storage
#19496115 - 01/30/14 12:01 PM (10 years, 11 months ago) |
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hey guys, looking for links, feedback, trials and tribulations for long term storage of cultures, edible and active. i see many different teks/containers/etc. was wondering what the pros and cons were to any of them? any of them last longer than others? are some easier to work with than others? for example snap on lids, screw on, plastic vials, glass vials, etc. i get paid in a week, so been reading up on it, and wanted user feedback from all you guys!
ps. not looking for a tek on how to do it, just looking for info on actual CONTAINERS people use and why
Thx in advance!
-------------------- A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!
The whole is greater than the sum of its parts
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slushie9090

Registered: 08/14/01
Posts: 2,000
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Re: long term storage *DELETED* [Re: blindingleaf]
#19498833 - 01/30/14 09:26 PM (10 years, 11 months ago) |
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Post deleted by Rin
Reason for deletion: .
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Sgt. Pepper



Registered: 06/19/13
Posts: 2,551
Loc: Third Stone From The Sun
Last seen: 3 months, 15 days
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If they're dry then a jar with a desiccant package. If they're wet, a paper bag in your fridge.
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MaJiK_420
...lost



Registered: 06/30/08
Posts: 447
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I think he said cultures.... not fruits....? Fruits yeah dehyrdrate, desicant and vacuum seal. Cultures.... not so much.
Long term storage of cultures I thought was done with a piece of wood in a test tube or something.
I bought some test tubes for that purpose, but I am still working on my Agar technique trying to get clean dishes and isolate good strains. I think they are called slants, because you store them at a slanted angle or something I can't remember. Haven't researched it lately.
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Re: long term storage [Re: MaJiK_420]
#19498922 - 01/30/14 09:49 PM (10 years, 11 months ago) |
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you can buy slant tubes. or look up yeast slant tubes. They're like 1-3$ a piece usually and sometimes come with a rack if you get them in a 10-20 pack
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ghiajake
Myco-Viking


Registered: 01/10/13
Posts: 3,957
Loc: Indiana
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Correct me if I'm wrong, but I think he's wanting to know the best way to preserve CULTURES, not fruits. The answer to that is by making culture slants. Glass or plastic is a debate that real is just up to personal preference. I prefer glass
Edited by ghiajake (01/30/14 09:52 PM)
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Sgt. Pepper



Registered: 06/19/13
Posts: 2,551
Loc: Third Stone From The Sun
Last seen: 3 months, 15 days
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Quote:
MaJiK_420 said: I think he said cultures.... not fruits....? Fruits yeah dehyrdrate, desicant and vacuum seal. Cultures.... not so much.
Long term storage of cultures I thought was done with a piece of wood in a test tube or something.
I bought some test tubes for that purpose, but I am still working on my Agar technique trying to get clean dishes and isolate good strains. I think they are called slants, because you store them at a slanted angle or something I can't remember. Haven't researched it lately.
You're right, my bad. I'm tired and I wasn't reading carefully. Quote:
bodhisatta said: you can buy slant tubes. or look up yeast slant tubes. They're like 1-3$ a piece usually and sometimes come with a rack if you get them in a 10-20 pack
I just bought 6 of those today at a homebrew store! They're cooling in the pc right now.
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Re: long term storage [Re: ghiajake]
#19498952 - 01/30/14 09:55 PM (10 years, 11 months ago) |
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you can really just use petri dishes that are wrapped too. Slants just take up less space and the cap is a little bit more of a precaution than a wrapped plate. Also a slant has a smaller opening so when you re-culture in your SAB it's some % less opportunity to introduce contams like why RR and some prefer the narrow mouth quarts to wide mouth quarts for grain jars. Petri dishes when used for re-culturing have to have more surface exposed every time you go back to it vs a slant tube. That's the other half reason, and they don't dry out or get condensation as easily
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Sgt. Pepper



Registered: 06/19/13
Posts: 2,551
Loc: Third Stone From The Sun
Last seen: 3 months, 15 days
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Quote:
bodhisatta said: you can really just use petri dishes that are wrapped too. Slants just take up less space and the cap is a little bit more of a precaution than a wrapped plate. Also a slant has a smaller opening so when you re-culture in your SAB it's some % less opportunity to introduce contams like why RR and some prefer the narrow mouth quarts to wide mouth quarts for grain jars. Petri dishes when used for re-culturing have to have more surface exposed every time you go back to it vs a slant tube. That's the other half reason, and they don't dry out or get condensation as easily
And you can add a small piece of wood to slants which extends their viability by years. I've read that storing cultures in sterile, distilled water causes the culture to shut down and "hibernate," so to speak. Doing this allows you to store a culture indefinitely.
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cronicr



Registered: 08/07/11
Posts: 62,905
Loc: Van Isle
Last seen: 32 minutes, 21 seconds
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Quote:
bodhisatta said: you can really just use petri dishes that are wrapped too. Slants just take up less space and the cap is a little bit more of a precaution than a wrapped plate. Also a slant has a smaller opening so when you re-culture in your SAB it's some % less opportunity to introduce contams like why RR and some prefer the narrow mouth quarts to wide mouth quarts for grain jars. Petri dishes when used for re-culturing have to have more surface exposed every time you go back to it vs a slant tube. That's the other half reason, and they don't dry out or get condensation as easily

-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: long term storage [Re: cronicr]
#19499738 - 01/31/14 03:58 AM (10 years, 11 months ago) |
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cool thx guys!
i have also read about petris being good in fridge for months. i have some that are 4 months old, i was planning to see if its still viable.
mainly just trying to get an opinion on what slants are best, or what vials are best, for the money and for their durability and longevity.
ghlajake, do u find the screw tops annoying to work with versus rubber stoppers? i should note ill be using an SAB, not a hood, if that makes any difference in ur opinion. i know its preference, but what is the reason u prefer glass over plastic?
and sgt pepper, i have read that article about distilled h20. JM mcdoogle (i think thats his posting name) had a thread with a link/article about it, and i remember something being in the archives or cultivation information section about it. i guess any vessel would work for that, even 1/2 pints?
like i said there are many options, and i have a week till next paycheck, so I'm just looking around at vendors, e bay, lab supply places, and of course (more importantly to me) user feedback here on the forums.
-------------------- A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!
The whole is greater than the sum of its parts
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nelekrele037
Stranger
Registered: 06/24/13
Posts: 10
Last seen: 10 years, 10 months
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dont think too much, try to work more. The spore sirynge is how i preserve my cultures. Longest run was more than 18 months in the fridge, and it still worked. A bit slower than the freshly made, but that wont matter too much if you have a pressure cooker. Spore prints become useless rather quick, and the preservation and usage of mycelial cultures is risky. Make a syringe, test it, mark it and leave it in the fridge. No need for bottles, dishes, glass etc.
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 7 months, 28 days
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Quote:
nelekrele037 said: dont think too much, try to work more. The spore sirynge is how i preserve my cultures. Longest run was more than 18 months in the fridge, and it still worked. A bit slower than the freshly made, but that wont matter too much if you have a pressure cooker.
Please tell me how having a pressure cooker is going to extend the life of a stored spore syringe.
Quote:
nelekrele037 said: Spore prints become useless rather quick
Says who?
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***
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Sgt. Pepper



Registered: 06/19/13
Posts: 2,551
Loc: Third Stone From The Sun
Last seen: 3 months, 15 days
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Re: long term storage [Re: PussyFart]
#19500257 - 01/31/14 08:20 AM (10 years, 11 months ago) |
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Quote:
PussyFart said:
Quote:
nelekrele037 said: dont think too much, try to work more. The spore sirynge is how i preserve my cultures. Longest run was more than 18 months in the fridge, and it still worked. A bit slower than the freshly made, but that wont matter too much if you have a pressure cooker.
Please tell me how having a pressure cooker is going to extend the life of a stored spore syringe. I think he was saying that a pc'd jar will give you a large window of opportunity against contams, so it doesn't matter that the spores might take a while.
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Quote:
nelekrele037 said: dont think too much, try to work more. The spore sirynge is how i preserve my cultures. Longest run was more than 18 months in the fridge, and it still worked. A bit slower than the freshly made, but that wont matter too much if you have a pressure cooker. Spore prints become useless rather quick, and the preservation and usage of mycelial cultures is risky. Make a syringe, test it, mark it and leave it in the fridge. No need for bottles, dishes, glass etc.
spore "sirynge" will last a hell of a lot longer than 18 months. Also they don't preserve your cultures at all, they preserve the fact that you have the spores for p.cubensis mushrooms. With those spores you can isolate a monoculture or start a culture to save.
I have never used a pressure cooker with a spore syringe, I also would love to know about this.
spore prints can last decades...
mycelium is not risky at all to preserve unless you're shitty at mycology.
While there's no NEED for dishes and glasses etc.. people use them to have more control and ensure 100% clean cultures. There's no need if you want to have a crapshoot of random potency fruits that flush randomly and look all different as well as having contamination be an inevitable percentage of your grow...
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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yea, just to clarify, i am talking about living cultures here, not spores. i have plates, i have agar, but i have no slants or test tubes.
-------------------- A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!
The whole is greater than the sum of its parts
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cronicr



Registered: 08/07/11
Posts: 62,905
Loc: Van Isle
Last seen: 32 minutes, 21 seconds
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your cultures are fine after four months, they will go blue if there dying
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: long term storage [Re: cronicr]
#19501502 - 01/31/14 02:13 PM (10 years, 11 months ago) |
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good to know, never saw blue myc!
-------------------- A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!
The whole is greater than the sum of its parts
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