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Offlineurthtown
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King Oyster Clones
    #19292562 - 12/18/13 09:39 AM (10 years, 2 months ago)

Bought a 3 pack of organic King Oysters from the grocery and took a tissue transfer from each of them. I did one directly to grain with a H202 dip first, another straight to grain with no H202 (I have a Tree Oyster clone growing out directly to grain quite happily) and a third to a sterilized jar of coffee soaked cardboard at field capacity. I'l be away for a week and a half so I'll see where everything is at when I get home and post an update.









Edited by urthtown (12/18/13 09:40 AM)


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Offlinet3chnobily
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Re: King Oyster Clones [Re: urthtown]
    #19293567 - 12/18/13 02:24 PM (10 years, 2 months ago)

My king is a store clone.  Great strain.  Good luck


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Offlinemrtumnas
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Re: King Oyster Clones [Re: t3chnobily]
    #19294576 - 12/18/13 06:31 PM (10 years, 2 months ago)

Tissue transfer straight to grain? I didn't know you could do that, lol...I always went Wild -> Agar -> Grain. I figured the contamination would be insane if you didn't isolate first.


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Offlinet3chnobily
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Re: King Oyster Clones [Re: mrtumnas]
    #19294644 - 12/18/13 06:43 PM (10 years, 2 months ago)

Agar is safer but good technique will usually result in clean biopsy.


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OfflineQueen of Kings
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Re: King Oyster Clones [Re: t3chnobily]
    #19295008 - 12/18/13 08:15 PM (10 years, 2 months ago)

Kings have such a fat stipe that it is pretty easy to tear one in two and grab a core sample that is totally pristine.  Assuming the rest of your conditions are super sterile I bet you won't have any problem.  Yes, agar would be safer. 

I have cloned grocery store Kings often with great results. 

Good luck!


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InvisibleZen Peddler
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Re: King Oyster Clones [Re: Queen of Kings]
    #19296464 - 12/19/13 03:51 AM (10 years, 2 months ago)

I got shot down initially here for suggesting that my two store bought clones of king oysters performed well and were in discernable from the Aloha strain on agar.
What I soon realized was that these people believed everything they read about grower genetics being expanded out to near senescence thus a clone will quickly decline or had simply never tried to grow out a market or store bought clone.
there is of course no chance that any commercial grower risks yields by heavy expansion of strain genetics.
and some commercial growers I know don't use commercial strains any more. I know an agaricus grower who runs a massive operation all using a strain isolated from a clone. :smile:
a one generation clone is unlikely to decline significantly if at all but all strains decline and can potentially throw off odd phenotypes.
I'm sure this grow will be fine.


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Offlinet3chnobily
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Re: King Oyster Clones [Re: Zen Peddler]
    #19297152 - 12/19/13 09:07 AM (10 years, 2 months ago)

cloned king



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Offlinethiotimoline
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Re: King Oyster Clones [Re: t3chnobily]
    #19297808 - 12/19/13 12:29 PM (10 years, 2 months ago)

Sweet! bluemeanie, I admit that I severely overgeneralized from my own blobby failure.


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InvisibleZen Peddler
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Re: King Oyster Clones [Re: thiotimoline]
    #19299095 - 12/19/13 05:34 PM (10 years, 2 months ago)

Haha. No probs.

I'm not a huge oyster fan in general but I love the flavor of the king oyster!


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OfflineQueen of Kings
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Re: King Oyster Clones [Re: Zen Peddler]
    #19300319 - 12/19/13 10:12 PM (10 years, 2 months ago)

Similarly pilfered Kings.  Definitely one of my favorite edibles. 



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Offlineurthtown
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Re: King Oyster Clones [Re: Queen of Kings]
    #19371843 - 01/05/14 08:53 AM (10 years, 1 month ago)

Hey guys sorry I missed the conversation here - was working on some other myco related projects and my king clones were tucked away in the cupboard for a couple weeks.

Well I lost one tissue to grain transfer and one took off just fine. Interestingly (anecdotally, not scientifically) I did two grain jars at the same time (see OP) and it was the one without the H202 dip that contammed. Could be coincidence, there's no real way to know where the contam came from. Looked like tiny green rough bubbles all over the grains. The cardboard clone is also growing happily if a bit wispy.





I shook the healthy one as it was at 30% colonized and it's recovering now so I think it will be ok to grow out on supplemented sawdust block as planned.

Great to see all the successful grows on grocery store genetics! My shiitake clone seems like it grew for a bit and then just gave up - thinking it may actually be close to senescence as it hasn't contammed, just stopped growing and shrivelled.

:rockon:


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Offlineurthtown
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Re: King Oyster Clones [Re: urthtown]
    #19471440 - 01/25/14 10:47 AM (10 years, 1 month ago)

Update!

I added some pasteurized hardwood sawdust to my cardboard clone jar after hydrating it with some coffee and gypsum. Looks happy! My wheat jar is fuzzing up but just slow slow slow. I think my grain might be too dry. I added 3 cc's of sterile water and noticed some improved mycelium growth.



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OfflineGordonWeasel
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Re: King Oyster Clones [Re: urthtown]
    #19471492 - 01/25/14 11:03 AM (10 years, 1 month ago)

I bought some from that same company and started the clones 12 days ago on little pieces of cardboard in petri dishes.  They are very slow as you said.  I cloned some grocery store grey oysters and they just started to pin today.  The price is right anyway.


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Offlineurthtown
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Re: King Oyster Clones [Re: GordonWeasel]
    #19472043 - 01/25/14 01:00 PM (10 years, 1 month ago)

Cool! I'm hoping to get some spores from a fruit on this sawdust to start sectoring a fresh isolate to grow out as a master culture. I have plenty to keep me busy with the isolates I have purchased so this is a fun side project. :reckon: Good luck.


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"All mushrooms are edible, but some only once."
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Offlineafk77
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Re: King Oyster Clones [Re: urthtown]
    #19475187 - 01/26/14 04:16 AM (10 years, 30 days ago)

ive never been able to fruit King Oysters, looks fun though.


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Offlineurthtown
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Re: King Oyster Clones [Re: afk77]
    #19475605 - 01/26/14 07:29 AM (10 years, 30 days ago)

Quote:

afk77 said:
ive never been able to fruit King Oysters, looks fun though.




Did you try a cold shock?


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"All mushrooms are edible, but some only once."
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InvisibleZen Peddler
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Re: King Oyster Clones [Re: urthtown]
    #19478781 - 01/26/14 08:32 PM (10 years, 29 days ago)

They are tasty!


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Offlineurthtown
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Re: King Oyster Clones [Re: Zen Peddler]
    #19500044 - 01/31/14 06:47 AM (10 years, 25 days ago)

Going to spawn one of these clones this weekend and I'll be prepping my substrate tomorrow.

What do you guys say - shredded straw log or supplemented sawdust block (supplemented with coffee, wheat germ and gypsum)?

Thanks! :rockon:


--------------------
Cluster Headache sufferer? Cluster Busting :thumbup:
Veil Tear GIF
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"All mushrooms are edible, but some only once."
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Offlinemycoloco
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Re: King Oyster Clones [Re: urthtown]
    #19500854 - 01/31/14 11:27 AM (10 years, 25 days ago)

After several frustrating attempts at fruiting kings, I have successfully settled on a mix of 1/3 straw, 2/3 sawdust supplemented with gypsum and finely ground brown rice. I find I get more fruits per pound by growing in jars -- scratch 43 days after inoculation, cold shock for 24 hours, set in pinning/fruiting environment of 55-60 degrees Fahrenheit, 8 hours of light per day.


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The universe is an illusion of the living.


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InvisibleGanzig
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Re: King Oyster Clones [Re: mycoloco]
    #19500876 - 01/31/14 11:33 AM (10 years, 25 days ago)

Strange. The kings I have start fruiting before I get a chance to put them
in fruiting conditions. Straw, coffee, or whatever. The straw bags and coffee bags
I did fruited in my living room with a few sprays from a spray bottle each day.

I had them fruiting off of a few left over grains in the bottom of a jar
once as well.

That original culture came from mycopath.com


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I must keep reminding myself of this. I must keep reminding myself of this.
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