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fractaldill
Cultivator in Training

Registered: 08/31/13
Posts: 909
Last seen: 10 years, 9 months
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Speed of Panaelous Cyanescens Mycelium
#19476625 - 01/26/14 12:41 PM (10 years, 11 months ago) |
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Does anyone have any experience with Pan Cyan mycelium and the rate at which it grows?
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cronicr



Registered: 08/07/11
Posts: 62,905
Loc: Van Isle
Last seen: 25 minutes, 34 seconds
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Re: Speed of Panaelous Cyanescens Mycelium [Re: fractaldill]
#19476960 - 01/26/14 01:51 PM (10 years, 11 months ago) |
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grows pretty quick in comnparison to cubes
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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fractaldill
Cultivator in Training

Registered: 08/31/13
Posts: 909
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Re: Speed of Panaelous Cyanescens Mycelium [Re: cronicr]
#19476994 - 01/26/14 01:59 PM (10 years, 11 months ago) |
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Really? Not the case with me
Panaelous Cyanescens

P. Galindoi ATL #7

P. Cubensis A+

All were inoculated the same day...
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fractaldill
Cultivator in Training

Registered: 08/31/13
Posts: 909
Last seen: 10 years, 9 months
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Re: Speed of Panaelous Cyanescens Mycelium [Re: fractaldill]
#19494542 - 01/30/14 12:29 AM (10 years, 11 months ago) |
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Is this "normal" or is this dish way too slow for a normal Pan Cyan variety?
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
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Re: Speed of Panaelous Cyanescens Mycelium [Re: fractaldill]
#19494833 - 01/30/14 03:14 AM (10 years, 11 months ago) |
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nice pics... 
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monoculture
Mycaddict



Registered: 10/16/13
Posts: 246
Last seen: 10 years, 5 months
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Re: Speed of Panaelous Cyanescens Mycelium [Re: spacechildo]
#19494920 - 01/30/14 03:51 AM (10 years, 11 months ago) |
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Pics removed after 3 days...
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fractaldill
Cultivator in Training

Registered: 08/31/13
Posts: 909
Last seen: 10 years, 9 months
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Re: Speed of Panaelous Cyanescens Mycelium [Re: monoculture]
#19495543 - 01/30/14 09:10 AM (10 years, 11 months ago) |
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huh? I still see them...
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fractaldill
Cultivator in Training

Registered: 08/31/13
Posts: 909
Last seen: 10 years, 9 months
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Re: Speed of Panaelous Cyanescens Mycelium [Re: fractaldill]
#19495657 - 01/30/14 09:57 AM (10 years, 11 months ago) |
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Here
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MacMerdin
Hunter



Registered: 04/26/12
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Re: Speed of Panaelous Cyanescens Mycelium [Re: fractaldill]
#19496189 - 01/30/14 12:24 PM (10 years, 11 months ago) |
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You can try soaking some poo (horse or cow) in water and using that water to mix your agar.
Also, I have found that grass seed water works too. My cincts and fimicola had a hard time colonizing regular MEA but when I used grass seed water, they covered the dish in a matter of days with pure white mycelium.
Experiment and let us know.
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fractaldill
Cultivator in Training

Registered: 08/31/13
Posts: 909
Last seen: 10 years, 9 months
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Re: Speed of Panaelous Cyanescens Mycelium [Re: MacMerdin]
#19496476 - 01/30/14 01:40 PM (10 years, 11 months ago) |
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Thanks for the tip! I will try that with my next batch of plates!
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fractaldill
Cultivator in Training

Registered: 08/31/13
Posts: 909
Last seen: 10 years, 9 months
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Re: Speed of Panaelous Cyanescens Mycelium [Re: MacMerdin]
#19498021 - 01/30/14 06:48 PM (10 years, 11 months ago) |
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Coming back. I would assume that this "trick" would work for a liquid culture as well correct?
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MacMerdin
Hunter



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Re: Speed of Panaelous Cyanescens Mycelium [Re: fractaldill]
#19498249 - 01/30/14 07:36 PM (10 years, 11 months ago) |
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Liquid cultures can be tricky directly from a spore print.
It is hard to tell cubensis myc in a LC let alone pan myc (which looks a lot like cobweb mold to begin with).
It's up to you but personally, I'd stay away from LC until I got agar down.
BTW.....you can shoot some sterile water from a syringe onto an agar dish, swirl around the needle to mix some myc and agar together and suck up the slurry. This creates a semi-liquid culture in the syringe (but one you know is contam free). It's how I've been doing my syringes lately.
Do this in a SAB, Glove Box, or under a hood.
Edited by MacMerdin (01/30/14 07:38 PM)
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cronicr



Registered: 08/07/11
Posts: 62,905
Loc: Van Isle
Last seen: 25 minutes, 34 seconds
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Re: Speed of Panaelous Cyanescens Mycelium [Re: MacMerdin]
#19498254 - 01/30/14 07:37 PM (10 years, 11 months ago) |
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thats how i've been doing mine as well
-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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MacMerdin
Hunter



Registered: 04/26/12
Posts: 2,068
Loc: The Island of Misfit Toys
Last seen: 3 months, 13 days
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Re: Speed of Panaelous Cyanescens Mycelium [Re: cronicr]
#19498286 - 01/30/14 07:44 PM (10 years, 11 months ago) |
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Great minds think alike.
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fractaldill
Cultivator in Training

Registered: 08/31/13
Posts: 909
Last seen: 10 years, 9 months
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Re: Speed of Panaelous Cyanescens Mycelium [Re: MacMerdin]
#19498407 - 01/30/14 08:08 PM (10 years, 11 months ago) |
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I was thinking about transferring a wedge of that plate to a LC. would you not recommend that? I do like that idea with the syringes though...
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GimpCollector
To Drunk To Taste The Chicken

Registered: 02/08/12
Posts: 1,097
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Re: Speed of Panaelous Cyanescens Mycelium [Re: MacMerdin]
#19498560 - 01/30/14 08:36 PM (10 years, 11 months ago) |
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Quote:
MacMerdin said: Liquid cultures can be tricky directly from a spore print.
It is hard to tell cubensis myc in a LC let alone pan myc (which looks a lot like cobweb mold to begin with).
It's up to you but personally, I'd stay away from LC until I got agar down.
BTW.....you can shoot some sterile water from a syringe onto an agar dish, swirl around the needle to mix some myc and agar together and suck up the slurry. This creates a semi-liquid culture in the syringe (but one you know is contam free). It's how I've been doing my syringes lately.
Do this in a SAB, Glove Box, or under a hood. 
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