Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   Left Coast Kratom Buy Kratom Extract   Unfolding Nature Unfolding Nature: Being in the Implicate Order   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   MagicBag.co All-In-One Bags That Don't Suck   Kraken Kratom Red Vein Kratom   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag   OlympusMyco.com We’re Not Chasing Unicorns—We’re Building Quality (Olympus Myco Grow Bags)

Jump to first unread post Pages: 1 | 2  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinearea5one
Stranger


Registered: 05/04/09
Posts: 18
Last seen: 9 years, 2 months
still-air box questions
    #19495801 - 01/30/14 10:34 AM (10 years, 11 months ago)

Hi all,

I made myself a still airbox with gloves attached to it - it looks pretty much the same as - https://files.shroomery.org/files/13-23/032853058-18.jpg (from a tek in the 'general cultivation' section)

These are my questions, any advice appreciated!

1. For dealing with contaminants I used soapy water on the sides and bottom, but the bottom of the box isn't flat though so the water runs off and dries up fast. Does this matter?

2. If using a flame inside the box, can this be a problem because it disturbs the air?

3. There is a different type of box design where tyvek sleeves are mounted in place of gloves ( https://files.shroomery.org/files/10-47/097450939-GBOverhead_POV.jpg - from a post by TranscendingLife). What are your opinions on which design is better?

Extras: Filter Print Post Top
OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 7 months, 28 days
Trusted Cultivator
Re: still-air box questions [Re: area5one]
    #19495851 - 01/30/14 10:45 AM (10 years, 11 months ago)

Quote:

area5one said:
I made myself a still airbox with gloves attached to it



That is a glove box, and is not recommended for this hobby.

Build  SAB(Still Air Box), not a glove box.

A GB(Glove Box) is not the same thing as a SAB(Still Air Box).

A SAB is better for this hobby than a GB because the gloves are attached to you and not the box.

Making it possible to flame sterilize your tool outside the box between transfers.

Also when people try to "seal" their GBs, the spots that they missed(like around the lid) will constantly suck air in and out with your movements.

This will negate the "still air environment"....

1. remove the lid and use the SAB upside down on a wet towel.

This will give you a nice floor to work with, and the towel will create sort of a "gasket".

2. Never flame inside the box, always flame outside. Flaming inside the box creates heat currents.

3. I use tyvek sleeves in my SAB.....my gloved hands go right thru them and into the box.

Extras: Filter Print Post Top
Offlinearea5one
Stranger


Registered: 05/04/09
Posts: 18
Last seen: 9 years, 2 months
Re: still-air box questions [Re: PussyFart]
    #19495924 - 01/30/14 11:03 AM (10 years, 11 months ago)

Hi - thanks PF, that does seem like good advice

So you just use plain water then with the towel? (no soap)

Do you disinfect the air inside the box in some way?

Extras: Filter Print Post Top
OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 7 months, 28 days
Trusted Cultivator
Re: still-air box questions [Re: area5one]
    #19495954 - 01/30/14 11:13 AM (10 years, 11 months ago)

I use a 5-10% bleach solution on my towel.

There is no need to disinfect the air, that is not the p;oint.

The point is to create a draft free, still air environment.

If the air is still and everything in the air has settled to the walls or bottom, nothing can possibly blow into your work.

Extras: Filter Print Post Top
Offlinesupernewfie
some fella
Male

Registered: 09/24/13
Posts: 1,103
Loc: Canada Flag
Last seen: 6 years, 4 months
Re: still-air box questions [Re: area5one]
    #19496233 - 01/30/14 12:37 PM (10 years, 11 months ago)



This is what I used for pouring agar(from a small pot w/ lid) to plates and innoculating the plates from syringe. 10 plates I poured and innocd, not 1 contamd before they dried or till I started fucking with them (towards the later end of dish viability) I innocd 2 outside the SAB, full of contams within days bacterial growth n couple weeks, green mold formed. needless to say i got rid of those 2 quickly after that. Anyways I just washed it out with hot water on a cloth, i never contamed a dish in it and i dont use gloves and i really dont see why youd need it.

Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery

Folding@home Statistics
Registered: 04/30/13
Posts: 61,915
Loc: Milky way
Trusted Cultivator
Re: still-air box questions [Re: supernewfie]
    #19496274 - 01/30/14 12:50 PM (10 years, 11 months ago)

Quote:

supernewfie said:


This is what I used for pouring agar(from a small pot w/ lid) to plates and innoculating the plates from syringe. 10 plates I poured and innocd, not 1 contamd before they dried or till I started fucking with them (towards the later end of dish viability) I innocd 2 outside the SAB, full of contams within days bacterial growth n couple weeks, green mold formed. needless to say i got rid of those 2 quickly after that. Anyways I just washed it out with hot water on a cloth, i never contamed a dish in it and i dont use gloves and i really dont see why youd need it.



Because some of us like to do things a little less half assed when working with sterile media

Extras: Filter Print Post Top
Invisiblebootster
Male User Gallery

Registered: 02/22/11
Posts: 1,531
Re: still-air box questions [Re: bodhisatta]
    #19496303 - 01/30/14 12:59 PM (10 years, 11 months ago)

I just place two large paper towels on the bottom of the SAB, wetted down, and work off of that. It keeps the contams contained and the dishes don't want to slide on the plastic to liquid surface. When I'm done, I just put them in the compost pile. YMMV.

Extras: Filter Print Post Top
Offlinesupernewfie
some fella
Male

Registered: 09/24/13
Posts: 1,103
Loc: Canada Flag
Last seen: 6 years, 4 months
Re: still-air box questions [Re: bodhisatta]
    #19496731 - 01/30/14 02:25 PM (10 years, 11 months ago)

well it worked :O

anyways im just stating the way I do it, as is other people will do it another way. the media was sterile when it went in, and sterile when it came out. lol im not gonna try to convince you to not go full ass with it but half ass worked fine for me. all im sayin.

Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery

Folding@home Statistics
Registered: 04/30/13
Posts: 61,915
Loc: Milky way
Trusted Cultivator
Re: still-air box questions [Re: supernewfie]
    #19496889 - 01/30/14 02:50 PM (10 years, 11 months ago)

Quote:

supernewfie said:
well it worked :O

anyways im just stating the way I do it, as is other people will do it another way. the media was sterile when it went in, and sterile when it came out. lol im not gonna try to convince you to not go full ass with it but half ass worked fine for me. all im sayin.




that was very similar to my first SAB you'll find yourself wanting to upgrade in a few months is my bet.:shrug:

Extras: Filter Print Post Top
Offlinearea5one
Stranger


Registered: 05/04/09
Posts: 18
Last seen: 9 years, 2 months
Re: still-air box questions [Re: bodhisatta]
    #19496953 - 01/30/14 02:59 PM (10 years, 11 months ago)

Quote:

I just place two large paper towels on the bottom of the SAB, wetted down, and work off of that. It keeps the contams contained and the dishes don't want to slide on the plastic to liquid surface. When I'm done, I just put them in the compost pile. YMMV.




This is a good tip for the setup that I'm running now!

Extras: Filter Print Post Top
Invisibleindocult
Dr
Male User Gallery

Registered: 07/12/09
Posts: 1,400
Re: still-air box questions [Re: area5one]
    #19497025 - 01/30/14 03:09 PM (10 years, 11 months ago)

I just put clean paper towels on a table, and put my box upside down on that.

gives a stable surface and more room.  much easier than trying to use the lid.

Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,870
Last seen: 2 months, 8 days
Trusted Cultivator
Re: still-air box questions [Re: supernewfie]
    #19497138 - 01/30/14 03:30 PM (10 years, 11 months ago)

Quote:

Anyways I just washed it out with hot water on a cloth, i never contamed a dish in it and i dont use gloves and i really dont see why youd need it.



Because of this

The purpose of the SAB it to protect the culture from you more than anything.


--------------------

Extras: Filter Print Post Top
OfflineHappy Littletree
One

Registered: 01/02/14
Posts: 1,386
Loc: slightly elevated from ea...
Last seen: 2 years, 2 months
Re: still-air box questions [Re: Kizzle]
    #19497221 - 01/30/14 03:46 PM (10 years, 11 months ago)

I read it takes like 40 minutes for the spores and stuff in the SAB to settle can anyone verify if this is true?

Extras: Filter Print Post Top
OnlinecronicrMFacebook
 User Gallery


Registered: 08/07/11
Posts: 62,905
Loc: Van Isle Flag
Last seen: 3 minutes, 25 seconds
Trusted Cultivator
OG Cultivator
Re: still-air box questions [Re: Happy Littletree]
    #19497226 - 01/30/14 03:47 PM (10 years, 11 months ago)

it's true, but thats y we spray with water to knock them dow, so spray and get strait to work


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

Extras: Filter Print Post Top
Invisiblebootster
Male User Gallery

Registered: 02/22/11
Posts: 1,531
Re: still-air box questions [Re: cronicr]
    #19497328 - 01/30/14 04:09 PM (10 years, 11 months ago)

I give it at least 10 min. That gives me time to go over my procedure one more time so that I'm not making more moves than necessary. The less moves, the better as far as stirring up contaminates goes. That's just my personal experience.

Extras: Filter Print Post Top
Invisibletbagtag
Boomer Barron

Registered: 01/16/13 Happy 12th Shroomiversary!
Posts: 1,432
Loc: Amsterdam Flag
Re: still-air box questions [Re: cronicr]
    #19497360 - 01/30/14 04:12 PM (10 years, 11 months ago)

Quote:

cronicr said:
it's true, but thats y we spray with water to knock them dow, so spray and get strait to work




Cavacide/Matacide for the win!  Whip that shit, wait 10 minutes, ?????, Profit!!!!

But for real 99% of the suggestions here will work, except for the guy who doesnt glove up.  Ive never gotten HIV from fucking raw dog but my odds go up each time I do it.  Just like contams in your tubs/cakes.

Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,870
Last seen: 2 months, 8 days
Trusted Cultivator
Re: still-air box questions [Re: cronicr]
    #19497429 - 01/30/14 04:24 PM (10 years, 11 months ago)

Assuming the air remains still even when you're not using the box there shouldn't be many contaminants airborne inside it to begin with. I do know though that water impacting the surface can send some contaminants back into the air.


--------------------

Extras: Filter Print Post Top
OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 7 months, 28 days
Trusted Cultivator
Re: still-air box questions [Re: Happy Littletree]
    #19497435 - 01/30/14 04:27 PM (10 years, 11 months ago)

Quote:

Happy Littletree said:
it takes like 40 minutes seconds for the spores and stuff in the SAB to settle



:thumbup:

Extras: Filter Print Post Top
Invisibletbagtag
Boomer Barron

Registered: 01/16/13 Happy 12th Shroomiversary!
Posts: 1,432
Loc: Amsterdam Flag
Re: still-air box questions [Re: PussyFart]
    #19497526 - 01/30/14 04:48 PM (10 years, 11 months ago)

Quote:

PussyFart said:
Quote:

Happy Littletree said:
it takes like 40 minutes 600 seconds for the spores and stuff in the SAB to settle



:thumbup:





I have training in air born pathogens and sterile room procedure for extreme body modifications.  I had to get certified through the state....

:thumbup:

Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery

Folding@home Statistics
Registered: 04/30/13
Posts: 61,915
Loc: Milky way
Trusted Cultivator
Re: still-air box questions [Re: tbagtag]
    #19498894 - 01/30/14 09:41 PM (10 years, 11 months ago)

10m seems more like it, I've watched dust settle in a still room with a sun beam coming through the window, took a few minutes but it's cool to see when the sun is just right the air is still enough after you smoke or something through the curtains. you can actually get a sense of how the air settles it's really quite still after about 3-4 minutes then everything starts to slowly fall.

Extras: Filter Print Post Top
OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 7 months, 28 days
Trusted Cultivator
Re: still-air box questions [Re: bodhisatta]
    #19498909 - 01/30/14 09:44 PM (10 years, 11 months ago)

Quote:

bodhisatta said:
10m seems more like it, I've watched dust settle in a still room with a sun beam coming through the window, took a few minutes but it's cool to see when the sun is just right the air is still enough after you smoke or something through the curtains. you can actually get a sense of how the air settles it's really quite still after about 3-4 minutes then everything starts to slowly fall.



I never have problems jumping right in after 30-40 seconds.

This is not a big room where dust has small drafts keeping it a float.

This is a small isolated space.....just wait a minute or 2 and start working.

Extras: Filter Print Post Top
Offlinearea5one
Stranger


Registered: 05/04/09
Posts: 18
Last seen: 9 years, 2 months
Re: still-air box questions [Re: PussyFart]
    #19500473 - 01/31/14 09:19 AM (10 years, 11 months ago)

This is my setup here now



One gentleman suggested, aka PussyFart (PF), turning the tub upside down, I think this turns out to be a really good idea (i) it gives a stable working surface (ii) things don't slide around inside the box (iii) it increases the working area because the top of the box is larger than the bottom, and (iv) I'm assuming contaminants will have a harder time getting in from a crack at the bottom rather than the top

I have some new questions now, if anyone would like to follow up on this it would be great

1. Is using a wet towel going to be enough to make contaminants settle on it, don't we need to provide a soapy film for them to stick onto? PF also talked about using bleach on the towel, raising the question on whether we are trying to kill the contaminants or trap them.

2. How important is it to sanitise the room that the work is going to take place in? (I've read some posts about people 'ousting' the room to death before getting started)

3. I'm still using gloves instead of tyvek sleeves, what are your opinions about the better choice? (Are gloves supposed to be bad because of pressure differences sucking in contaminants??)

Extras: Filter Print Post Top
OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 7 months, 28 days
Trusted Cultivator
Re: still-air box questions [Re: area5one]
    #19500482 - 01/31/14 09:24 AM (10 years, 11 months ago)

1. I use a 5-10% bleach soaked towel, and it seems to trap everything that falls on it.(cannot know this for sure, but I have a very high success rate)(It makes sense in theory)

2. Not important. Just clean the immediate area.....no sense in cleaning the ceiling if working in a SAB, but clean air is a good thing.(if that made any sense lol)

3. Cut the gloves off the box and wear them, that way you can move in and out of the box freely to flame sterilize your tools.

Edited by PussyFart (01/31/14 09:25 AM)

Extras: Filter Print Post Top
OfflineKizzle
Misanthrope
 User Gallery

Registered: 08/30/11
Posts: 9,870
Last seen: 2 months, 8 days
Trusted Cultivator
Re: still-air box questions [Re: PussyFart]
    #19500691 - 01/31/14 10:46 AM (10 years, 11 months ago)

Quote:

1. Is using a wet towel going to be enough to make contaminants settle on it, don't we need to provide a soapy film for them to stick onto? PF also talked about using bleach on the towel, raising the question on whether we are trying to kill the contaminants or trap them.



We spray the box to more quickly remove the contaminants from the air. Even without a wet surface on the bottom they'd have a hard time becoming airborne after they've settled although leaving it wet is probably beneficial in trapping larger particles like dust which carrying contaminants and become airborne much more easily. It's the surface tension of the water that causes the trapping effect so it doesn't necessarily need to be soapy water.


--------------------

Extras: Filter Print Post Top
Offlinesupernewfie
some fella
Male

Registered: 09/24/13
Posts: 1,103
Loc: Canada Flag
Last seen: 6 years, 4 months
Re: still-air box questions [Re: tbagtag]
    #19501198 - 01/31/14 12:53 PM (10 years, 11 months ago)

Quote:

tbagtag said:
Quote:

cronicr said:
it's true, but spray with water to knock them dow, so spray and get strait to work




Cavacide/Matacide for the win!  Whip that shit, wait 10 minutes, ?????, Profit!!!!

But for real 99% of the suggestions here will work, except for the guy who doesnt glove up.  Ive never gotten HIV from fucking raw dog but my odds go up each time I do it.  Just like contams in your tubs/cakes.





Thats why I shower, and just before using throughly wash my hands. I flame sterilize my tools I use inside the SAB and those dishes ive done up aswell as the wedge cuttings to transfer, even though ive never done much what I did do never contamd yet. So explain how it "doesnt" work?

And in reality your odds do not go up for getting HIV with the more women you have sex with "raw dog" ,  all you have to do is pick the wrong slut and you got that shit its more of a poor judgment(or poor hygene) issue rather than the percentage and chance. Now you could be right if the women were all hookers off the street... then youre just playing russian roulette, bound to bite the bullet.

Edited by supernewfie (01/31/14 01:10 PM)

Extras: Filter Print Post Top
Invisiblebootster
Male User Gallery

Registered: 02/22/11
Posts: 1,531
Re: still-air box questions [Re: supernewfie]
    #19501458 - 01/31/14 02:05 PM (10 years, 11 months ago)

I've had good success putting the tyvek sleeves outside the box, rubber bands to the opening and spraying iso on the sleeves. It seems to work for me ATM.

Extras: Filter Print Post Top
Invisibleblindingleaf
blue collar underworld
 User Gallery


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
Trusted Cultivator
Re: still-air box questions [Re: bootster]
    #19501652 - 01/31/14 02:41 PM (10 years, 11 months ago)

i used those yellow dish gloves not attached and it worked.  when they cracked after a long time, i just used food service gloves, that came up to wrist, still worked well.  slow movements, smart movements, good results.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

Extras: Filter Print Post Top
Invisiblebootster
Male User Gallery

Registered: 02/22/11
Posts: 1,531
Re: still-air box questions [Re: bootster]
    #19502239 - 01/31/14 04:53 PM (10 years, 11 months ago)

:whathesaid:

Extras: Filter Print Post Top
Jump to top Pages: 1 | 2  [ show all ]

Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   Left Coast Kratom Buy Kratom Extract   Unfolding Nature Unfolding Nature: Being in the Implicate Order   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   MagicBag.co All-In-One Bags That Don't Suck   Kraken Kratom Red Vein Kratom   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag   OlympusMyco.com We’re Not Chasing Unicorns—We’re Building Quality (Olympus Myco Grow Bags)


Similar ThreadsPosterViewsRepliesLast post
* Glove Box Question The_Mushroom_God 4,367 6 04/30/02 10:51 PM
by bluepoo
* GLOVE BOX QUESTION (HEPA OR NOT TO HEPA) hyper_dermic 2,244 8 07/17/02 11:55 PM
by hyper_dermic
* pos. pressure glove box question Hafrican 1,350 3 02/05/03 09:39 PM
by BikeCourier
* grow box question blaat 1,073 8 11/14/02 07:14 PM
by Skikid16
* Glove Box..... DannyBoy 4,949 17 06/30/06 05:55 PM
by djInfctdMushroom
* question about grain 2 grain innoculation robinhood2 2,453 12 09/22/02 01:17 PM
by matts
* G-box, New TEk? Old Tek? New Twist, U tellMe! NewbieShroomer 1,811 4 11/12/01 02:26 PM
by NewbieShroomer
* Re: Eq mycelium question (pics) Anonymous 3,801 19 05/27/00 05:08 PM
by Anonymous

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
5,472 topic views. 33 members, 110 guests and 28 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.027 seconds spending 0.008 seconds on 12 queries.