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seand04
It is what it is



Registered: 12/02/13
Posts: 1,457
Last seen: 4 months, 1 day
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Agar
#19495321 - 01/30/14 07:34 AM (10 years, 11 months ago) |
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So im going to start with agar and i was wondering if i should use pre poured dishes or do it myself. Would pre poured be more venurable to contams?
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bluecap
mychanical



Registered: 11/10/13
Posts: 286
Last seen: 9 years, 4 months
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do it yourself man. its really pretty easy. those prepours are expensive. all depends on how the plates are prepared concerning contam issues...have fun... agar is the only way to go in my opinion...
-------------------- I wish I was a grain of sand, layin in a babies hand...fallin like a diamond chain into the ocean... A willow tree is strong enough to bend, never like the oak who lives in fear of a wind....Gamma... 
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Stargaze
Stranger



Registered: 11/11/13
Posts: 120
Loc: Sporeville
Last seen: 10 years, 8 months
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Others can feel free to disagree with me but my first box of agar powder included sugar in the ingredients. I think this can hose up the performance of the agar. I recommend getting straight up agar with nothing else added.
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seand04
It is what it is



Registered: 12/02/13
Posts: 1,457
Last seen: 4 months, 1 day
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Ok I was think of buying pre poured from amazon because they are really cheap
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seand04
It is what it is



Registered: 12/02/13
Posts: 1,457
Last seen: 4 months, 1 day
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bluecap
mychanical



Registered: 11/10/13
Posts: 286
Last seen: 9 years, 4 months
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yep, don't get the kind with sugar in it. prob work with plain agar. you can add nutes such as instant potatoe flakes, honey or light corn syrup, malt extract, yeast, ect... the myc gets lazy if you add too many nutes. it grows faster with low nutes, searching for food.... or if you have the expenible cash. get the prepours for ease for your first try...I,m a cheapass so I make my own...
-------------------- I wish I was a grain of sand, layin in a babies hand...fallin like a diamond chain into the ocean... A willow tree is strong enough to bend, never like the oak who lives in fear of a wind....Gamma... 
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 7 years, 4 months
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lots of people complain that prepoured is pre-contamed when it comes in the mail
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cli_hlt
Beshroomed



Registered: 10/29/11
Posts: 463
Loc: EU
Last seen: 7 days, 14 minutes
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Pre-poured dishes are clean, sometimes even contain antibiotics to help your business, but yes, they are quite expensive.
With home made dishes you can stay cheap even if you screw up and have contamination rates like 50%.
Also, you can do agar in a SAB and have reasonable success rate. With a decent technique you can approach 100% while staying low-tech.
Unfortunately agar is very unforgiving. One wrong move can cause MOST of your plates to show quite colourful growths in days. It can be frustrating, but worth it.
Colonizing agar plates a beautiful, and great tools to get pure cultures, stable yields, to identify and work around small contaminations.
Always stay as clear as possible. Constantly revise your technique. Always think ahead, be quick, and reflect afterwards: did I do it right? Where could be any crew-ups? How could I avoid those situations next time?
I think agar is awesome.
Good luck!
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seand04
It is what it is



Registered: 12/02/13
Posts: 1,457
Last seen: 4 months, 1 day
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Yeah i was thinking because its my first go with agar to buy pre poured so I dont fuck it up. but they also have one that comes w the powder dishes and swabs for 20 or 30 so I might do that.
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seand04
It is what it is



Registered: 12/02/13
Posts: 1,457
Last seen: 4 months, 1 day
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This is what i was thinking I would do. Take a nice cap. make a spore print . Colonize agar w print. Move agar to jars. and well you know the rest.
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seand04
It is what it is



Registered: 12/02/13
Posts: 1,457
Last seen: 4 months, 1 day
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Im gonna do some research first so if anyone has any good links let me know.
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Stargaze
Stranger



Registered: 11/11/13
Posts: 120
Loc: Sporeville
Last seen: 10 years, 8 months
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Quote:
seand04 said: This is what i was thinking I would do. Take a nice cap. make a spore print . Colonize agar w print. Move agar to jars. and well you know the rest.
Again I'm a novice so I may be wrong here, but I believe it's "better" to colonize using a biopsy from a nice fruit with good traits vs. spores. You want to work with good genetics.
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seand04
It is what it is



Registered: 12/02/13
Posts: 1,457
Last seen: 4 months, 1 day
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Ok but all of my spores come from a very reliable source always nice fruits. But i am planning on using the nicest one.
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cli_hlt
Beshroomed



Registered: 10/29/11
Posts: 463
Loc: EU
Last seen: 7 days, 14 minutes
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Mushrooms tend to mutate quite quickly.
If you clone too much, their genetics mutate to a point where the vigor of the strain will be lost and yield and/or potency will be diminished. Susceptibility to infections will grow, as colonization times increase. This is called senescence.
Because of the quick mutations, spores from a single fruit can show sometimes surprising genetic variety. This is why growing multispore cultures said to be like gambling. Mostly average, sometimes bad, sometimes great.
If I wanted to select for certain traits, I's grow dozens if not hundreds of MS bags and search for that trait.
If you find the perfect strain, you can always clone the life out of it - quite literally.
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