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OfflineDSkank
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Registered: 10/23/13
Posts: 6
Last seen: 1 year, 8 months
PF cakes - no pins - stroma? overlay? contam? - need advice!!
    #19491198 - 01/29/14 12:24 PM (10 years, 11 months ago)

fairly new grower here, done a couple grows with pf cakes, yet to have any really outstanding results :/

to begin the story, these 3 cakes were left in jars for about a month after full colonization since i was away for a few weeks and wouldnt have had time to fruit them completely before leaving.

after returning, i removed the cakes, dunked and rolled and placed in fc. after a few days i noticed each one had grown mold (i think trich based on pics seen here) on the top of the cake (bottom of the jar) where the dry vermiculite had failed to stick and mycelium was more exposed. having waited so long i was reluctant to throw them out despite the obvious contam (stupid, i know) so i wiped my gloves and an exacto with alcohol wipes, put on a breathing mask and sliced off the rim of the top where the contamination was seen, then placed the cakes outside on my patio for a few hours.

after taking them back inside and placing them back in the chamber, after a few days i figured i would have seen the trich sporulate again based on how fast it had before, if it was still there.at this point i re dunked and re-rolled. this time i tried pasteurizing the vermiculite (bag of verm was suspect) and adding some lime, however this meant it was soaked when i rolled the cakes.

after placing them in the fc i covered it with a bag until the next day to allow the mycelium to colonize the layer more quickly. after seeing some growth i thought it better to introduce fruiting conditions sooner to give the mycelium more of a chance against the trich, if it was still battling.

while the cakes were still in the fc i drilled holes in each side to make a sgfc design, a mistake i realized when i read here that petroleum fumes can cause stroma growth (if that's what this is?) i noticed the more normal looking growth i had seen before kinda died down while the stroma(?) continued to grow. it's now been almost 3 days since i made the sgfc and exposed to fruiting conditions, there are no pins. here are some pics of the cakes:





i've been misting and fanning about 4 or 5 times every day, 12/12 light cycle with a 5000k bulb (couldn't find anything higher that wasn't a tube) elevating the fc on 4 small jars to give the bottom holes some air (no it's not on my floor) around the middle of my room. hygrometer in the fc reads low 90s to 95. i've tried calibrating it multiple times with the wet paper towel and damp salt methods and it seems to be working (though it's about -15% off, and i don't want to try and turn the dial again for fear of ruining it)

id like to know is this stroma? overlay? i would think trich wouldve sporulated by now if that's what this is.
if it's stroma, what i've read here would suggest it's pretty much screwed in terms of producing fruits, right? is there anything i can do with it? use it as spawn? (i do have ingredients for coir/verm sub) outdoor bed? scrape it off with a sterile blade?
i know i've made some definitely noob mistakes but, absolutely anything i could do for a chance at seeing fruits, any advice or knowledge please post!! im trying to learn as much as i can about this but i just don't know what to do at this point.

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OfflineCamtaro420
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Registered: 11/25/13
Posts: 707
Loc: New Mexico
Last seen: 6 years, 5 months
Re: PF cakes - no pins - stroma? overlay? contam? - need advice!! [Re: DSkank]
    #19491246 - 01/29/14 12:38 PM (10 years, 11 months ago)

Damn man. Not to be the bearer of bad news but there was just a lot done wrong...

My advice is to bury them in the garden with some manure and start from scratch.

There are some excellent deals on syringes right now.

Edit: Oh, and never try to remove contaminates other then cobweb(with H202). Never.


--------------------

Edited by Camtaro420 (01/29/14 12:39 PM)

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OfflinecronicrMFacebook
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Re: PF cakes - no pins - stroma? overlay? contam? - need advice!! [Re: Camtaro420]
    #19491251 - 01/29/14 12:40 PM (10 years, 11 months ago)

never sit your cakes right on the perlite and get the bw out of there


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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OfflineDSkank
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Registered: 10/23/13
Posts: 6
Last seen: 1 year, 8 months
Re: PF cakes - no pins - stroma? overlay? contam? - need advice!! [Re: cronicr]
    #19491466 - 01/29/14 01:34 PM (10 years, 11 months ago)

bw?

they have jar lids underneath

id prefer some explanation to just being told that i did it wrong, which i already know

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OfflinecronicrMFacebook
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Re: PF cakes - no pins - stroma? overlay? contam? - need advice!! [Re: DSkank]
    #19491486 - 01/29/14 01:42 PM (10 years, 11 months ago)

get the hygrometer out of there, it's not needed for a sgfc, as long as you mist when the surface drys that gives you your 100% humidity on the surface of your cakes as it evaporates and also where your hygrometer can't measure so don't worry about humididty, and overlay won't happen with cubes so just forget the word all together lol, and no it's not stroma either although it may be a bacterial contam.
i wrap my jar rings in foil to sit them on, how are there moisture levels?
i think they will fruit if you give them time they donm't look in too bad of shape


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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OfflineDSkank
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Registered: 10/23/13
Posts: 6
Last seen: 1 year, 8 months
Re: PF cakes - no pins - stroma? overlay? contam? - need advice!! [Re: cronicr]
    #19491982 - 01/29/14 03:47 PM (10 years, 11 months ago)

thanks cronicr, i appreciate your posting again. if i look at the perlite where the light shines on it i can see that it's moist, but there are very few droplets visible against the inside of the plastic. i usually try to spray from a height so that a fine mist falls on the cakes. also they are only sitting on the flat part of the lid, no ring. would it be better to use something that elevates them?

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OfflinecronicrMFacebook
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Re: PF cakes - no pins - stroma? overlay? contam? - need advice!! [Re: DSkank]
    #19492098 - 01/29/14 04:07 PM (10 years, 11 months ago)

it's just less messy that way


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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