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Offlinebiglo
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Interesting idea?
    #1949093 - 09/24/03 08:30 PM (13 years, 2 months ago)

Well, I was mulling over how one would go about doing a fairly pure psilocybin extraction the other night. I didn't get to far, because I remembered, psilly rabbit, oxygen would react with it and oxidize it into psilocin. Well I thought, how could I remove the oxygen from the atmosphere to prevent oxidization? Well, I could replace the oxygen with an inert gas, such as CO2. Now this probably isn't a new concept, but the bright idea I had was instead of messing with dry ice, there is a much cheaper and effective alternative, at least in theory. If anyone is familiar with paintball, they would know that CO2 propels the balls from the marker. CO2 tanks can be easily and cheaply obtained at any walmart in various sizes, and CO2 refills are relatively cheap if one has a place to fill tanks near them. My idea would be to rig a rubbermaid box up similarly to a glove box, only putting greater emphasis on making sure it is airtight. Follow my explanation in the diagram below:



A valve aquired at a hardware store for little money is attached to one end of the box, while a sealable vent is installed on the other end. Basically the CO2 valve is opened, allowing CO2 gas into the chamber and displaces the oxygen, which exit out the vent. When the proper CO2 level is reached inside the box, the vent is sealed, and a CO2 filled environment is created. Proper CO2 levels can be roughly checked by attempting to light a lighter in the box. If the lighter won't light, no oxygen is present, and it's safe to procede with an extraction in the CO2 environment. This method would be great for better psilocybin extractions, as well as a better preservation method for long term storage, all at an inexpensive and relatively easy setup. Let me know what ya think!


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InvisibleOsker246
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Re: Interesting idea? [Re: biglo]
    #1949563 - 09/24/03 10:43 PM (13 years, 2 months ago)

Damn thats pretty interesting. I don't know if it would work or not so I don't think I should make any comment about it. But very creative thinking keep it up.



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Offlineprocule
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Re: Interesting idea? [Re: Osker246]
    #1949662 - 09/24/03 11:08 PM (13 years, 2 months ago)

The psilocybin isn't oxidized.
The psilocin is.

Psilocybin is quite stable and you should not bother that much for an extraction.


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Offlinebiglo
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Re: Interesting idea? [Re: procule]
    #1949772 - 09/24/03 11:42 PM (13 years, 2 months ago)

Damnit! I hate it when I confuse substances and terminology! Anyways, the point is not only for doing better extractions, but also for doing long term storage. I've read a report saying shroom powder from dried fruits has been kept for years while still retaining potency. This method would hopefully be able to extend shelf life with less degredation, without making an elaborate setup.


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InvisibleArsey
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Re: Interesting idea? [Re: biglo]
    #1949841 - 09/25/03 12:11 AM (13 years, 2 months ago)

I dunno.. seems the atmosphere would be primed for anearobic bacteria. Helium or cfc's would probably work but what if the oxidation is more of something that occurs on a endogeneous enzymatic level. If free radicals within the cellular system facilitate the breakdown via some enzymatic process then the atmosphere may be insignificant. In fact, if the oxidation is induced by stress the anearobic condition may actually promote the advance of the oxidative state.

Lemme put it this way.... If a mushroom was bruised in a atmosphere of pure CO2 would it still blue?


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OfflineRogerRabbitM
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Re: Interesting idea? [Re: Arsey]
    #1950233 - 09/25/03 03:20 AM (13 years, 2 months ago)

The wine industry uses a mixture of carbon dioxide, argon, and nitrogen. It seems to cover all the bases.


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Offlinebiglo
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Re: Interesting idea? [Re: biglo]
    #1950293 - 09/25/03 04:21 AM (13 years, 2 months ago)

That reminds me!  The more elite paintballers also used compressed air which is mostly nitrogen.  Don't know if that would affect it or not.  Anyways, to the person that said it would be prime for anerobic bacteria, I'm assuming that the extractions/preservations are being done on dried shrooms.  Life needs water, so if there was no humidity in the shrooms themselves, then there shouldn't be any bacterial growth, because the CO2 gas shouldn't "THEORETICALLY" contain water vapor.  But maybe some kind of preservative could also be introduced that wouldn't react with the mushies and is non toxic?  I dunno, I'm just spouting ideas at this point, damn ephedrine, been up all night on it trying to get this paper done for the am, sigh...  :sad: 


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OfflineMykeOfile
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Re: Interesting idea? [Re: biglo]
    #1951147 - 09/25/03 03:09 PM (13 years, 2 months ago)

F#<%!^& tweeker


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OfflinePsiloman
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Re: Interesting idea? [Re: MykeOfile]
    #1951155 - 09/25/03 03:15 PM (13 years, 2 months ago)

Nice idea.....Insted of bying tanks of gases,why not burn the oxygen in the glovebox using a candle? I think this would be ok for the purposes of extraction,even storage.

I wrote a small tek utilising this method named "Psilomans storage tek" or smth like that...Run a search if u are interested


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InvisibleMilkVein
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Re: Interesting idea? [Re: Psiloman]
    #2030660 - 10/21/03 11:56 PM (13 years, 1 month ago)

that is a good idea!....becarefull with the candle though...you dont want to loose all your hair!


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OfflinePsily_Rabbit
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Let them suffocate themselves! [Re: MilkVein]
    #2030832 - 10/22/03 12:30 AM (13 years, 1 month ago)

Using a lighter would be cheap and easy (plus less work). Just use one of those long snouted lightser you use for lighting gas grills.

Personally, I like using them for fireworks and burning ants.


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OfflineFlux
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Re: Let them suffocate themselves! [Re: Psily_Rabbit]
    #2032175 - 10/22/03 01:35 PM (13 years, 1 month ago)

Argon or helium if pretty cheap and doesn't react w/ a damn thing (99% of the time anyways). Argon is better because it's heavier then air and will more readily displace reactive gases whereas helium may spend a lot of time at the top of your chamber. Peace, Flux


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Invisiblemycofile
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Re: Let them suffocate themselves! [Re: Flux]
    #2036454 - 10/23/03 03:46 PM (13 years, 1 month ago)

Good theory here, but do you really want to do an extraction which will involve highly volatile solvents in an enclosed, non-vented box? I'm not sure I would. Once that rubbermaid filled with vapors, it will start building pressure (assuming it's sealed tight, which it would have to be for your idea to work well). The slightest leak or the slightest spark, and you've got a hell of a bomb on your hands. A large tupperware full of concentrated/compressed explosive vapors does not seem a safe thing to have around IMO.

-just a thought.


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