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OfflineFactorNMR2
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Registered: 07/29/03
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Last seen: 10 years, 1 month
Colonization
    #1949003 - 09/24/03 08:03 PM (14 years, 1 day ago)

Is it possible to colonize a jar with a piece of crumbled cake, allowing someone to make many jars out of just one? I would think so but a really havent read anything about it. Any answers appreciated.


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InvisibleSolarFlare
Lady Flare

Registered: 09/01/03
Posts: 194
Loc: LaLa Land
Re: Colonization [Re: FactorNMR2]
    #1949028 - 09/24/03 08:12 PM (14 years, 1 day ago)

Of course but youd problably get contams


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OfflineFactorNMR2
person
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Re: Colonization [Re: SolarFlare]
    #1949193 - 09/24/03 08:55 PM (14 years, 1 day ago)

What about if a glovebox is used and extra precautions are taken, does anyone think then contamination would be minimized?


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InvisibleMagashM
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Re: Colonization [Re: FactorNMR2]
    #1949211 - 09/24/03 08:59 PM (14 years, 1 day ago)

Put some brf in a oven bag and pc that. Then crumble up 1 cake per bag and colonize a bunch like that.

That tek is by Eatualive and he's done it many times.


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Invisibledysphoria
lost soul

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Registered: 06/04/03
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Re: Colonization [Re: FactorNMR2]
    #1949219 - 09/24/03 09:01 PM (14 years, 1 day ago)

assuming a glove box was used with sterile procedures, yes...then the risk of contamination would be minimized.

the method that best suits what you're wanting to do is culture innoculations, assuming you have a syringe lying around, which in this hobby, you should at some point.

http://www.shroomery.org/index/par/23545

i feel the culture syringe would be more effective, both in sterility and speed of colonization. no proof to back up why it is or isnt, only saying that a liquid medium injected into jars, ala spore syringe injection...would give rise to mycellium growth quicker than crumbling a piece and letting it take a foothold from there.

-jb


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OfflineFactorNMR2
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Re: Colonization [Re: dysphoria]
    #1949252 - 09/24/03 09:10 PM (14 years, 1 day ago)

Thanks plenty, the technique that looks most useful in this situation is Uncle Lazlo's Syringe tek.

http://www.shroomery.org/index/par/8002

Any opinions or comments. By the way, there is two fully colonized jars. What should be done with the rest of the cakes, and most of all..
WHERE DO YOU GET SYRINGES!?!?!


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InvisibleSixCee
keep rolling
Male

Registered: 06/12/02
Posts: 3,720
Loc: US, Chicago
Re: Colonization [Re: FactorNMR2]
    #1949273 - 09/24/03 09:16 PM (14 years, 1 day ago)

Those guys pretty much told you all you needed to know. I shall warn you that after several (3 or more) generations of transferring mycelium the genetics will get all screwy. You will sooner or later end up with mutants like abinos or something.


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->Quote of the Moment :
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OfflineFactorNMR2
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Re: Colonization [Re: SixCee]
    #1949297 - 09/24/03 09:20 PM (14 years, 1 day ago)

wow, magash responded to one of my posts, i love you on CC man, your a king...thanks


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InvisibleSixCee
keep rolling
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Re: Colonization [Re: FactorNMR2]
    #1949303 - 09/24/03 09:21 PM (14 years, 1 day ago)

That he is.


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~~~~~~~~~~~~~~~~~~~~~~~~
-->The above statements may or not be true.
->Quote of the Moment :
"Yea. All bitches are whores who love sex." -Cubie
----> PMs checked daily.


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OfflineFactorNMR2
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Posts: 42
Last seen: 10 years, 1 month
Re: Colonization [Re: SixCee]
    #1949311 - 09/24/03 09:22 PM (14 years, 1 day ago)

oh yea, where do i get syringes, can i get up at a drug store like cvs or something?


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OfflineFactorNMR2
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Registered: 07/29/03
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Re: Colonization [Re: FactorNMR2]
    #1949368 - 09/24/03 09:36 PM (14 years, 1 day ago)

Also, can you do a karo or honey using crumbled cake, and magash, no myco bag no pc.


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OfflineBaby_Hitler
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Re: Colonization [Re: FactorNMR2]
    #1949479 - 09/24/03 10:09 PM (14 years, 23 hours ago)

Also, you should read up more on peroxide, peroxidated grains, h2o2, etc...



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InvisibleSixCee
keep rolling
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Registered: 06/12/02
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Re: Colonization [Re: Baby_Hitler]
    #1949494 - 09/24/03 10:16 PM (14 years, 23 hours ago)

Baby Hitler your avatar!


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~~~~~~~~~~~~~~~~~~~~~~~~
-->The above statements may or not be true.
->Quote of the Moment :
"Yea. All bitches are whores who love sex." -Cubie
----> PMs checked daily.


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Offlinerockstafarian
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Registered: 08/22/03
Posts: 165
Last seen: 12 years, 1 month
Re: Colonization [Re: SixCee]
    #1949805 - 09/24/03 11:56 PM (14 years, 21 hours ago)

i want some mutated fruits! albinos would be so badass.


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^ Above post is complete fiction. ^


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OfflineBaby_Hitler
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Re: Colonization [Re: SixCee]
    #1950415 - 09/25/03 06:48 AM (14 years, 14 hours ago)

Papaver found it for me.  :smile2: :eyemouth: :nut: :smile2:


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Amazon Shop: ½ Pint Jars, Brown Rice Flour, Papaver Somniferum

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