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Dip41ea
Stranger

Registered: 12/22/13
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Last seen: 6 years, 9 months
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Incubate jars in warm water?
#19488208 - 01/28/14 07:42 PM (10 years, 11 months ago) |
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Hi,
First time growing here and was wondering whether it was possible/advisable to incubate brf jars in warm water. I have read the posts about just using room temp, but am concerned given how cold the weather is.
I don't have a standard aquarium or light I could use, but what I do have is a 5 gallon plastic bucket (typically used for homebrew) and a "belt" that goes around the bucket and keeps it at 80 degrees. However, the bucket and belt-warmer were designed to be used with water in the bucket and I'm not certain if there is much of a heating benefit with the jars just sitting in there. Would it be safe to add a little water to warm up, and in turn the water will warm the jars up? Of course, the water level would be well below the level that could creep into the jars.
Any advice is greatly appreciated!
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cronicr



Registered: 08/07/11
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Re: Incubate jars in warm water? [Re: Dip41ea]
#19488212 - 01/28/14 07:43 PM (10 years, 11 months ago) |
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if you don't need a jacket inside your house room temp is fine
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Rewindicus
Silly Goose



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Re: Incubate jars in warm water? [Re: cronicr]
#19488358 - 01/28/14 08:11 PM (10 years, 11 months ago) |
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Rr has incubated jars to 100% in his fridge just to see if it would work. It did. Put em in a closet an check on em in 10 days.
-------------------- “Be who you are and say what you feel because those who mind don't matter and those who matter don't mind.”- Dr. Seuss
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GimpCollector
To Drunk To Taste The Chicken

Registered: 02/08/12
Posts: 1,097
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Re: Incubate jars in warm water? [Re: Rewindicus]
#19488399 - 01/28/14 08:19 PM (10 years, 11 months ago) |
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BRF jars do not need to be incubated unless you are an inuit.
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Dip41ea
Stranger

Registered: 12/22/13
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Last seen: 6 years, 9 months
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I do understand that an incubator is not an absolute necessity and they will incubate at room temperature and even lower. But will they incubate at the same rate, or will they incubate faster if in mid 70s, even if just by a day?
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GimpCollector
To Drunk To Taste The Chicken

Registered: 02/08/12
Posts: 1,097
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Re: Incubate jars in warm water? [Re: Dip41ea]
#19493048 - 01/29/14 06:40 PM (10 years, 11 months ago) |
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Multi spore syringe I am guessing. That's like asking how long a piece of string is. To hard to tell with MS.
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Re: Incubate jars in warm water? [Re: Dip41ea]
#19493050 - 01/29/14 06:41 PM (10 years, 11 months ago) |
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The chances of them stalling out and going slower and contaming are higher especially with BRF cakes which are usually inoculated with a spore syringe which is hardly clean compared to agar work. Don't encourage the nasties. Think about an incubator when you have big spawn bags and live in northern canada. Cakes make some of their own heat too so if you're incubating at 75 You could be taking them to a temperature at which they go 2/3 as fast as they would have.
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refried

Registered: 06/14/13
Posts: 3,675
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Re: Incubate jars in warm water? [Re: bodhisatta]
#19493234 - 01/29/14 07:10 PM (10 years, 11 months ago) |
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My cakes have always worked from ms syringes even at 60-65 temp. Colonization took a month, however.
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Happy Littletree
One

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Re: Incubate jars in warm water? [Re: refried]
#19493286 - 01/29/14 07:24 PM (10 years, 11 months ago) |
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Incubating directly in water sounds like contamination heaven.. I am in the process of building an incubator myself but not just because of the cold but because of extreme shifts in temperature due to poor insulation and the use of kerosene heat. It forced me to place them in a colder area away from all the heat sources. I would maybe try using a smaller amount of water and put a bucket in the bucket forcing the water up the sides if you intend on trying something with it. Thats basically what I've done but I'm using two totes with one bolted to the other forcing the water up the sides. Hopefully this will stabilize my temps because before that just a few inches off the floor of my incubator it dropped temp by twenty degrees. Hope I don't have to use it long but I wanna get going sooner than later!! 
Edited by Happy Littletree (01/29/14 07:45 PM)
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BittrBuffalo
Deaconica

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Re: Incubate jars in warm water? [Re: bodhisatta]
#19493435 - 01/29/14 07:53 PM (10 years, 11 months ago) |
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I've tried it a number of ways, and I've found that incubation can be helpful in the winter. It's 68 F in my house, and they tend to do better at around 74-78 F. They'll still colonize, it's just going to take a bit longer. The jars will retain their own heat to some degree. In the past, I've used a little aquarium heater set to 80 F in a tub of water, with a smaller tub that would contain the jars (or whatever) set inside. I haven't had any problems with contam this way. Agar really isn't fond of 68 degree weather and I've taken to wrapping my plates in a sweatshirt and putting them near the heat from the vent. 65 F is too cold for anything meaningful to happen, so I'd incubate at that temperature myself. Incubating is kind of a pain in the ass and wastes electricity, so don't do it if you don't need to.
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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look up frank's tub in a tub incubator TIT should be easy to find. But seriously for cakes you don't need to incubate. Syringes are filthy relative to agar, and most people cant resist looking at their jars every day and picking them up etc... Don't encourage the contams that are more likely than they would be in a agar>spawn>SAB/FH grow.
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BittrBuffalo
Deaconica

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Re: Incubate jars in warm water? [Re: bodhisatta]
#19493503 - 01/29/14 08:04 PM (10 years, 11 months ago) |
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Oh shit, I hadn't seen Frank's tub in tub thing. In fact, I figured he'd totally take a dump on the idea of incubating at all.
-------------------- Disclaimer: This post is a work of fiction, provided for entertainment purposes only. Any resemblance to actual persons or events, past or present, is strictly coincidental. All celebrity voices are impersonated. If you begin your ID request with, "I just ate a bunch of these mushrooms…should I not have done that?" I'm just gonna sit back and watch Darwin at work.
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Happy Littletree
One

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Re: Incubate jars in warm water? [Re: bodhisatta]
#19493596 - 01/29/14 08:25 PM (10 years, 11 months ago) |
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Thanks for the lead.. Like I said I wouldn't have wasted the time to build it after reading on here but my temps fluctuate from upper 40's to near 90 degrees. The tek definitely had some good pointers in there. I hope to be doing some agar work as well starting from a MS and cleaning it up later hopefully some pins to agar and isolates. It sounds like the extra heat will definitely help on the agar part from what he says. One step at a time Speaking of which has anyone developed an easy way to cut tyvex ge disk filters.. I was thinking of folding the tyvek over and over and over placing between two boards and screwing those together to hold the fabric tight then drilling a one inch hole saw through it.. any thoughts on this?
Edited by Happy Littletree (01/29/14 08:31 PM)
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Re: Incubate jars in warm water? [Re: BittrBuffalo]
#19493600 - 01/29/14 08:28 PM (10 years, 11 months ago) |
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Quote:
BittrBuffalo said: Oh shit, I hadn't seen Frank's tub in tub thing. In fact, I figured he'd totally take a dump on the idea of incubating at all. 
incubation is worthwhile most big mushroom farms use some sort of temperature control in the room IE incubation, if you're at home and you can't then it's a good idea for spawn bags and lots of grain jars. If youre doing only a few grain jars or cakes though it's pretty much completely not worth it and maybe even a hindrance.
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Dip41ea
Stranger

Registered: 12/22/13
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Re: Incubate jars in warm water? [Re: bodhisatta]
#19510224 - 02/02/14 10:07 AM (10 years, 11 months ago) |
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Thanks for the feedback. The potential contam issue was my biggest concern. I ended up not using water as it turns out I didn't need it to maintain a constant temperature-apparently the "warmer" belt I put around my tub is enough to heat the space since it is so small.
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