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magickspore



Registered: 12/11/12
Posts: 798
Loc: Center of the universe.
Last seen: 7 months, 8 days
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Re: Plastic Tek Without Aluminum Foil?? [Re: blindingleaf]
#19497550 - 01/30/14 04:58 PM (10 years, 11 months ago) |
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Quote:
blindingleaf said: yea i raise my grate up with 3 glass 1/2 pint jars. magic, are u just doing agar at 10psi?
I would like to clarify my previous post. I do ten because my weight only holds 10-11psi. I will be buying a weight for 15 soon but can't at this moment. I haven't had a contamination because of my sterilization tek yet. When I DO get one I will add time. I don't expect others to follow suit. I got to these times outta trial and error and they work for me. When explaining the process to noobs I still advocate 15psi for 30-40min (agar).
I'm still honing in my times for wbs. At the moment I tried 2 and 1/2 hrs at 10psi (2 pp5 and 1 jar). I expect them to fail but they are also to wet so I'm throwing them out anyway.
For agar and jars at this moment I don't cram or double stack (maybe 1 on top). When I do load up its only a few. My time increases 10ish min for each agar container I add in. And like 30-45min per jar. Then I slowly just bring the time down till I get a contam in a control. Sometimes when I'm nervous I have multiple controls.
Since this has gone on long enough I'd like to add. I take a while to bring the water to a boil. I also take the time (15 min) to make sure I have a PC full of steam before I put my weight on. And again this is what works for me so results may vary.
Edited by magickspore (01/30/14 05:17 PM)
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SynapseLotus


Registered: 01/18/14
Posts: 130
Loc: Δx = ħ/(2•Δp)
Last seen: 9 years, 5 months
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Re: Plastic Tek Without Aluminum Foil?? [Re: magickspore]
#19498845 - 01/30/14 09:28 PM (10 years, 11 months ago) |
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Magickspore:
I have the AA 915 that holds 15 quarts. The thing is a dream compared to doing all those complicated processes to get a good result, especially having to do such small loads at a time.
I got mine off amazon and then had a friend bring it on the plane here (They also don't sell any AA PCs where I live). I think the total was around $180. For as much time as you spend doing all that stuff, just get one and you'll wonder why you didn't just buy one in the first place.
-------------------- See my shadow changing, Stretching up and over me.
Soften this old armor, hoping I can clear the way.
By stepping through my shadow, coming out the other side.
Step into the shadow, 46 and 2 are just ahead of me.
Spongiform's Plastic Tek
My gift to the Shroomery
Stan Getz/Bill Evans Masterpiece
The most important video of this generation
NO CUT, PERFECT MONOTUB HOLES!
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SynapseLotus


Registered: 01/18/14
Posts: 130
Loc: Δx = ħ/(2•Δp)
Last seen: 9 years, 5 months
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Re: Plastic Tek Without Aluminum Foil?? [Re: SynapseLotus]
#19503462 - 01/31/14 09:13 PM (10 years, 11 months ago) |
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Just checked today and a little over half of my 50+ containers are growing myc! It took about 5 days to start growth, but I don't see any contams so that is a good sign.
I got a bit impatient and re-inoc'ed about 20 of them after 4 days, so I'll have some weird mix of what comes up between Equadorian and B+. I wonder which will prevail...
Even the first ones I noc'ed up (with only about half a CC in each of 2 holes) are growing well after about 7 days.
Cheers all!
-------------------- See my shadow changing, Stretching up and over me.
Soften this old armor, hoping I can clear the way.
By stepping through my shadow, coming out the other side.
Step into the shadow, 46 and 2 are just ahead of me.
Spongiform's Plastic Tek
My gift to the Shroomery
Stan Getz/Bill Evans Masterpiece
The most important video of this generation
NO CUT, PERFECT MONOTUB HOLES!
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RandomFX
protege


Registered: 12/02/13
Posts: 1,015
Loc: North-East, USA
Last seen: 6 years, 1 month
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Re: Plastic Tek Without Aluminum Foil?? [Re: SynapseLotus]
#19503679 - 01/31/14 10:07 PM (10 years, 11 months ago) |
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I use (used) the pp5's with screw on lids, and mine do not even haze or flex after they have been pc'd full on at 15 psi for 90 minutes.
pp5 isn't any plastic container you get your hands on and pee pee # 5 on the chinese receipt.
personally though I'd use a pour recipe from PC'in the agar, pouring in a SAB in sterile petri's. my gosh I love how they look so pristine, and clear (though I've been adding a half drop of food dye to the agar making it all awesome looking)...I just like staring at them honestly... I love them. So pretty...my precious....but the pp5's do work, and re-usable.
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SynapseLotus


Registered: 01/18/14
Posts: 130
Loc: Δx = ħ/(2•Δp)
Last seen: 9 years, 5 months
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Re: Plastic Tek Without Aluminum Foil?? [Re: RandomFX]
#19504713 - 02/01/14 04:43 AM (10 years, 11 months ago) |
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Quote:
pp5 isn't any plastic container you get your hands on and pee pee # 5 on the chinese receipt.
-------------------- See my shadow changing, Stretching up and over me.
Soften this old armor, hoping I can clear the way.
By stepping through my shadow, coming out the other side.
Step into the shadow, 46 and 2 are just ahead of me.
Spongiform's Plastic Tek
My gift to the Shroomery
Stan Getz/Bill Evans Masterpiece
The most important video of this generation
NO CUT, PERFECT MONOTUB HOLES!
Edited by SynapseLotus (02/01/14 05:05 AM)
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