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Offlinefractaldill
Cultivator in Training

Registered: 08/31/13
Posts: 909
Last seen: 10 years, 9 months
Looking for recommendations
    #19483224 - 01/27/14 07:51 PM (10 years, 11 months ago)

I'm new the world of agar, and im liking quite a lot. Ive been pondering what i should do with my inoculated plates, and I think ive concluded that I will work on isolating by making 3 transfers from one dish and work on them in 3 sectioned petri dishes. With the rest of the plate I will inoculate a grain jar (good idea?)

Any other recommendations?

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InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
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Registered: 04/30/13
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Loc: Milky way
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Re: Looking for recommendations [Re: fractaldill]
    #19483244 - 01/27/14 07:54 PM (10 years, 11 months ago)

that works, do what you want. you don't have to find an isolate. Isolates are a waist of time unless you fruit them all out to save the good one. I would always use old agar plates as inoculation when trying to limit the genetics more. One of these days I'll find an awesome culture good enough to save for a long time. Otherwise the fun is just playing the game.

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Offlinefractaldill
Cultivator in Training

Registered: 08/31/13
Posts: 909
Last seen: 10 years, 9 months
Re: Looking for recommendations [Re: bodhisatta]
    #19483316 - 01/27/14 08:06 PM (10 years, 11 months ago)

The possibilities with agar are so overwhelming! lol

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Offlinefractaldill
Cultivator in Training

Registered: 08/31/13
Posts: 909
Last seen: 10 years, 9 months
Re: Looking for recommendations [Re: bodhisatta]
    #19483341 - 01/27/14 08:12 PM (10 years, 11 months ago)

So with this perspective in mind. Instead of worrying about isolating I can just inoculate another plate or two, use those for masters, maybe make a LC, and proceed from there?

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InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
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Folding@home Statistics
Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Re: Looking for recommendations [Re: fractaldill]
    #19483375 - 01/27/14 08:20 PM (10 years, 11 months ago)

yep, I would advise against LC but if you're making one from agar you should be ok and good to go. You still introduce needles to your grow which are a vector for contams but you'll be way more likely to have a good one than a bad one.

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