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OfflineTwisted_kitten
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HELP! Should I throw this jar out?! PICS!!!
    #19482407 - 01/27/14 05:14 PM (10 years, 11 months ago)

Okay so I checked on my jars and one of them has been looking kinda weird for a little while but no smell like the other jars I had to toss.  I have a little baby shroom on the side of the jar that looks healthy but the other side is kinda yellow brown.  Take a look and let me know what I should do!!  Thanks!!


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Anything I post in this forum is strictly for entertainment purposes and should not be taken literal.

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InvisibleGanzig
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Re: HELP! Should I throw this jar out?! PICS!!! [Re: Twisted_kitten]
    #19482522 - 01/27/14 05:43 PM (10 years, 11 months ago)

Looks like you have a bit o' bacteria.

Personally I would not keep it.

One jar of mycelium is nothing.


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OfflineVE3HPC
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Re: HELP! Should I throw this jar out?! PICS!!! [Re: Ganzig]
    #19482561 - 01/27/14 05:51 PM (10 years, 11 months ago)

bacteria


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-"It's all been done before. You're just another brain going through another depression. Accept it, treat it, move on."

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OfflineTwisted_kitten
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Re: HELP! Should I throw this jar out?! PICS!!! [Re: Ganzig]
    #19482930 - 01/27/14 07:05 PM (10 years, 11 months ago)

Bummer.... I only had 3 jars survive out of 8.  All bacteria contaminated....but the other jars had an odor that's why I was hesitant to throw this one out.  Especially if it was pinning.  But thank you for your comments!!!    This is my first grow so I'm still learning!


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OfflineVE3HPC
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Re: HELP! Should I throw this jar out?! PICS!!! [Re: Twisted_kitten]
    #19482977 - 01/27/14 07:12 PM (10 years, 11 months ago)

All of my BRF jars that are incubated (not just put on a shelf at room temp) seem to always have a noticeable funky bacterial-like smell coming through the holes on top of the verm barrier. If I DON'T incubate (I have only done a few jars like that) and set the jars in a closet on a shelf, the smell is never there. I guess that's bacteria favoring stale air and higher temps. I guess I have been lucky that no bacteria spread to the rest of my BRF cakes. I have only had one issue with contam before in one jar, and I chucked it immediately.


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-"It's all been done before. You're just another brain going through another depression. Accept it, treat it, move on."

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OfflineKingCloyster
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Re: HELP! Should I throw this jar out?! PICS!!! [Re: VE3HPC]
    #19483086 - 01/27/14 07:29 PM (10 years, 11 months ago)

What method did you use to sterilize and inoculate the jars? :crazy2:


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OfflineTwisted_kitten
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Re: HELP! Should I throw this jar out?! PICS!!! [Re: KingCloyster]
    #19485108 - 01/28/14 07:54 AM (10 years, 11 months ago)

Kingcloyster - I used a small bathroom.  I cleaned and bleached the hell out of it....took down shower curtains and removed anything from the bathroom that I could.  Closed the vent.  Wore gloves.  Flamed the needle after every couple jars.  I may try either doing it Dexter style next time or using a box.  Any other suggestions?  I'm thinking of starting another batch since this one wasnt as successful as I had hoped.

Oh and I boiled the jars with the substrate for about an hour beforehand.  Let them cool for about 5-6 hours


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OfflineKingCloyster
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Re: HELP! Should I throw this jar out?! PICS!!! [Re: Twisted_kitten]
    #19485359 - 01/28/14 09:27 AM (10 years, 11 months ago)

Ive had almost success using a simple still air box with two arm holes cut out.  Nothing fancy, no tyvek sleeves, although that helps. Wipe down the inside with ISO alc, spray the inside once more time with alc and start loading jars in one by one with gloved hands. Be sure to wipe down each jar with ISO alc before u load it in. And then when they are inside the box, take the foil off the jars right before u flame sterilize the needle and inoculate each one. BE CAREFUL! I HAVE LIT MY ISO SOAKED HANDS ON FIRE BEFORE! Let the alc evaporate quite a bit


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OfflineMAIA
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Re: HELP! Should I throw this jar out?! PICS!!! [Re: Twisted_kitten]
    #19488977 - 01/28/14 10:15 PM (10 years, 11 months ago)

You guys might be right but it's a hard call on this one. It could be a bacterial contam or metabolites. I would give 4 or 5 days and see if it colonizes 100%. You won't be loosing more than you already have but yeah ... probably a gonner.


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Use, do not abuse; neither abstinence nor excess ever renders man happy.
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OfflineKingCloyster
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Re: HELP! Should I throw this jar out?! PICS!!! [Re: MAIA]
    #19489053 - 01/28/14 10:36 PM (10 years, 11 months ago)

Isn't early fruiting a sign of a bacterial contam?

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Offlinemycopanda
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Re: HELP! Should I throw this jar out?! PICS!!! [Re: Twisted_kitten]
    #19489074 - 01/28/14 10:40 PM (10 years, 11 months ago)

Quote:

Twisted_kitten said:
Kingcloyster - I used a small bathroom.  I cleaned and bleached the hell out of it....took down shower curtains and removed anything from the bathroom that I could.  Closed the vent.  Wore gloves.  Flamed the needle after every couple jars.  I may try either doing it Dexter style next time or using a box.  Any other suggestions?  I'm thinking of starting another batch since this one wasnt as successful as I had hoped.

Oh and I boiled the jars with the substrate for about an hour beforehand.  Let them cool for about 5-6 hours



If your boiling you need to do it for at the very least 90 mins..


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OfflineMAIA
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Re: HELP! Should I throw this jar out?! PICS!!! [Re: KingCloyster]
    #19503741 - 01/31/14 10:20 PM (10 years, 11 months ago)

Quote:

KingCloyster said:
Isn't early fruiting a sign of a bacterial contam?




It is and that's why I think it's a gonner, but invitro pinning might be triggered by other factors as well. Even if full mycelial consolidation was not achieved :crazy:


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Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
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Offlinemycopanda
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Re: HELP! Should I throw this jar out?! PICS!!! [Re: MAIA]
    #19504191 - 01/31/14 11:59 PM (10 years, 11 months ago)

Jars also pin early because they make a perfect micro climate needed for pinning..


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OfflineKingCloyster
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Re: HELP! Should I throw this jar out?! PICS!!! [Re: mycopanda]
    #19505927 - 02/01/14 12:15 PM (10 years, 11 months ago)

Quote:

MAIA said:
Quote:

KingCloyster said:
Isn't early fruiting a sign of a bacterial contam?




It is and that's why I think it's a gonner, but invitro pinning might be triggered by other factors as well. Even if full mycelial consolidation was not achieved :crazy:



Quote:

mycopanda said:
Jars also pin early because they make a perfect micro climate needed for pinning..




Wow, ya learn somethin new everyday on this site :P


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