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Offlinematt1208
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agar agar
    #19479743 - 01/27/14 12:33 AM (10 years, 11 months ago)

what is it about agar agar that works in petri dishes, is it just a thickener or does it help with growth?


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Offlinefractaldill
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Re: agar agar [Re: matt1208]
    #19479756 - 01/27/14 12:39 AM (10 years, 11 months ago)

This is just my speculation, Im not too sure. I believe agar agar acts as a gelatinous media, added to the agar mix will be something such as light malt extract or potato dextrose. These compounds are what is nutritious for the mycelium.

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OfflineHappy Littletree
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Re: agar agar [Re: fractaldill]
    #19479793 - 01/27/14 01:05 AM (10 years, 11 months ago)

:whathesaid:Agar is used to provide a solid surface containing medium for the growth of bacteria and fungi. Microbial growth does not destroy the gel structure because most microorganisms are unable to digest agar. Other ingredients are added to the agar to meet the nutritional needs of the microbes.- wiki

Edited by Happy Littletree (01/27/14 01:09 AM)

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OnlinecronicrMFacebook
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Re: agar agar [Re: Happy Littletree]
    #19479911 - 01/27/14 02:35 AM (10 years, 11 months ago)

gives us a 2d advantage making strain isolation possible


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Offlinematt1208
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Re: agar agar [Re: cronicr]
    #19479994 - 01/27/14 03:35 AM (10 years, 11 months ago)

so could brown rice flour be used? sorry for the noob questions I just have no understanding of using agar


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OnlinecronicrMFacebook
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Re: agar agar [Re: matt1208]
    #19479996 - 01/27/14 03:36 AM (10 years, 11 months ago)

yes it can it's just not nearly as effective for things like strain isolation but you cand get clean cultures and clones with it


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Offlinemagickspore
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Re: agar agar [Re: matt1208]
    #19479997 - 01/27/14 03:37 AM (10 years, 11 months ago)

Yes someone has done that before recently. I just can't remember who...

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Invisiblemushmagic
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Re: agar agar [Re: matt1208]
    #19480000 - 01/27/14 03:38 AM (10 years, 11 months ago)

Quote:

matt1208 said:
so could brown rice flour be used? sorry for the noob questions I just have no understanding of using agar




Search "BRF paste" or "agar substitute". It works in a pinch but doesn't have the advantage of isolating like agar does.


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OnlinecronicrMFacebook
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Re: agar agar [Re: magickspore]
    #19480002 - 01/27/14 03:39 AM (10 years, 11 months ago)

Quote:

magickspore said:
Yes someone has done that before recently. I just can't remember who...



i did dummy lol


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It doesn't matter what i think of you...all that matters is clean spawn
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Offlinemagickspore
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Re: agar agar [Re: cronicr]
    #19480009 - 01/27/14 03:41 AM (10 years, 11 months ago)

Quote:

cronicr said:
Quote:

magickspore said:
Yes someone has done that before recently. I just can't remember who...



i did dummy lol




:rofl: I know I was just being a smart ass.

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OnlinecronicrMFacebook
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Re: agar agar [Re: magickspore]
    #19480012 - 01/27/14 03:42 AM (10 years, 11 months ago)

lol ok i thought your were thinking of blindingleaf who also did it lol


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It doesn't matter what i think of you...all that matters is clean spawn
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Offlinemagickspore
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Re: agar agar [Re: cronicr]
    #19480018 - 01/27/14 03:45 AM (10 years, 11 months ago)

Quote:

cronicr said:
lol ok i thought your were thinking of blindingleaf who also did it lol




Really? I gotta start paying attention better.

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Offlinematt1208
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Re: agar agar [Re: magickspore]
    #19480040 - 01/27/14 03:54 AM (10 years, 11 months ago)

ok well I'll search that and give it a go :smile:


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Offlinemagickspore
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Re: agar agar [Re: matt1208]
    #19480053 - 01/27/14 03:58 AM (10 years, 11 months ago)

Yea look in Cronicr journal or for his 50 dollar grow for some other ideas or help.

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OnlinecronicrMFacebook
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Re: agar agar [Re: magickspore]
    #19480055 - 01/27/14 03:59 AM (10 years, 11 months ago)

or click my main threads for the active version


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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Offlinematt1208
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Re: agar agar [Re: cronicr]
    #19480072 - 01/27/14 04:18 AM (10 years, 11 months ago)

Quote:

cronicr said:
or click my main threads for the active version




was a good read :smile: sorry to hear about your troubles but I gotta say if my girl said that to me I'd look her in the eyes take her by the hand and walk her to the door :p haha


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