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Offlinefractaldill
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Speed of Panaelous Cyanescens Mycelium
    #19476625 - 01/26/14 12:41 PM (10 years, 11 months ago)

Does anyone have any experience with Pan Cyan mycelium and the rate at which it grows?

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OfflinecronicrMFacebook
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Re: Speed of Panaelous Cyanescens Mycelium [Re: fractaldill]
    #19476960 - 01/26/14 01:51 PM (10 years, 11 months ago)

grows pretty quick in comnparison to cubes:thumbup:


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It doesn't matter what i think of you...all that matters is clean spawn
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Offlinefractaldill
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Re: Speed of Panaelous Cyanescens Mycelium [Re: cronicr]
    #19476994 - 01/26/14 01:59 PM (10 years, 11 months ago)

Really? Not the case with me :frown:

Panaelous Cyanescens


P. Galindoi ATL #7


P. Cubensis A+


All were inoculated the same day...

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Offlinefractaldill
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Re: Speed of Panaelous Cyanescens Mycelium [Re: fractaldill]
    #19494542 - 01/30/14 12:29 AM (10 years, 11 months ago)

Is this "normal" or is this dish way too slow for a normal Pan Cyan variety?

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Offlinespacechildo
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Re: Speed of Panaelous Cyanescens Mycelium [Re: fractaldill]
    #19494833 - 01/30/14 03:14 AM (10 years, 11 months ago)

nice pics... :rolleyes:

:magicfingers:

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Offlinemonoculture
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Re: Speed of Panaelous Cyanescens Mycelium [Re: spacechildo]
    #19494920 - 01/30/14 03:51 AM (10 years, 11 months ago)

Pics removed after 3 days... :frown:

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Offlinefractaldill
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Re: Speed of Panaelous Cyanescens Mycelium [Re: monoculture]
    #19495543 - 01/30/14 09:10 AM (10 years, 11 months ago)

huh? I still see them...

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Offlinefractaldill
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Re: Speed of Panaelous Cyanescens Mycelium [Re: fractaldill]
    #19495657 - 01/30/14 09:57 AM (10 years, 11 months ago)

Here

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OfflineMacMerdin
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Re: Speed of Panaelous Cyanescens Mycelium [Re: fractaldill]
    #19496189 - 01/30/14 12:24 PM (10 years, 11 months ago)

You can try soaking some poo (horse or cow) in water and using that water to mix your agar.

Also, I have found that grass seed water works too.  My cincts and fimicola had a hard time colonizing regular MEA but when I used grass seed water, they covered the dish in a matter of days with pure white mycelium. 

Experiment and let us know.


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Offlinefractaldill
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Re: Speed of Panaelous Cyanescens Mycelium [Re: MacMerdin]
    #19496476 - 01/30/14 01:40 PM (10 years, 11 months ago)

Thanks for the tip! I will try that with my next batch of plates!

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Offlinefractaldill
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Re: Speed of Panaelous Cyanescens Mycelium [Re: MacMerdin]
    #19498021 - 01/30/14 06:48 PM (10 years, 11 months ago)

Coming back. I would assume that this "trick" would work for a liquid culture as well correct?

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OfflineMacMerdin
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Re: Speed of Panaelous Cyanescens Mycelium [Re: fractaldill]
    #19498249 - 01/30/14 07:36 PM (10 years, 11 months ago)

Liquid cultures can be tricky directly from a spore print.

It is hard to tell cubensis myc in a LC let alone pan myc (which looks a lot like cobweb mold to begin with).

It's up to you but personally, I'd stay away from LC until I got agar down.

BTW.....you can shoot some sterile water from a syringe onto an agar dish, swirl around the needle to mix some myc and agar together and suck up the slurry.  This creates a semi-liquid culture in the syringe (but one you know is contam free).  It's how I've been doing my syringes lately. 

Do this in a SAB, Glove Box, or under a hood.  :thumbup:


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Edited by MacMerdin (01/30/14 07:38 PM)

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OfflinecronicrMFacebook
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Re: Speed of Panaelous Cyanescens Mycelium [Re: MacMerdin]
    #19498254 - 01/30/14 07:37 PM (10 years, 11 months ago)

thats how i've been doing mine as well:thumbup:


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I'm tired do me a favor

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OfflineMacMerdin
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Re: Speed of Panaelous Cyanescens Mycelium [Re: cronicr]
    #19498286 - 01/30/14 07:44 PM (10 years, 11 months ago)

Great minds think alike.  :thumbup:


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Offlinefractaldill
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Re: Speed of Panaelous Cyanescens Mycelium [Re: MacMerdin]
    #19498407 - 01/30/14 08:08 PM (10 years, 11 months ago)

I was thinking about transferring a wedge of that plate to a LC. would you not recommend that? I do like that idea with the syringes though...

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InvisibleGimpCollector
To Drunk To Taste The Chicken

Registered: 02/08/12
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Re: Speed of Panaelous Cyanescens Mycelium [Re: MacMerdin]
    #19498560 - 01/30/14 08:36 PM (10 years, 11 months ago)

Quote:

MacMerdin said:
Liquid cultures can be tricky directly from a spore print.

It is hard to tell cubensis myc in a LC let alone pan myc (which looks a lot like cobweb mold to begin with).

It's up to you but personally, I'd stay away from LC until I got agar down.

BTW.....you can shoot some sterile water from a syringe onto an agar dish, swirl around the needle to mix some myc and agar together and suck up the slurry.  This creates a semi-liquid culture in the syringe (but one you know is contam free).  It's how I've been doing my syringes lately. 

Do this in a SAB, Glove Box, or under a hood.  :thumbup:



:thumbup:

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