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urthtown
meat popsicle


Registered: 10/26/13
Posts: 1,039
Loc: Eastern Canadia
Last seen: 2 years, 4 months
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King Oyster Clones
#19292562 - 12/18/13 09:39 AM (10 years, 2 months ago) |
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Bought a 3 pack of organic King Oysters from the grocery and took a tissue transfer from each of them. I did one directly to grain with a H202 dip first, another straight to grain with no H202 (I have a Tree Oyster clone growing out directly to grain quite happily) and a third to a sterilized jar of coffee soaked cardboard at field capacity. I'l be away for a week and a half so I'll see where everything is at when I get home and post an update.



Edited by urthtown (12/18/13 09:40 AM)
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t3chnobily
Strangest


Registered: 02/04/12
Posts: 651
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Re: King Oyster Clones [Re: urthtown]
#19293567 - 12/18/13 02:24 PM (10 years, 2 months ago) |
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My king is a store clone. Great strain. Good luck
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mrtumnas
Oyster-licious



Registered: 05/15/10
Posts: 186
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Tissue transfer straight to grain? I didn't know you could do that, lol...I always went Wild -> Agar -> Grain. I figured the contamination would be insane if you didn't isolate first.
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t3chnobily
Strangest


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Re: King Oyster Clones [Re: mrtumnas]
#19294644 - 12/18/13 06:43 PM (10 years, 2 months ago) |
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Agar is safer but good technique will usually result in clean biopsy.
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Queen of Kings
Get on with the Fascination



Registered: 12/11/11
Posts: 1,201
Loc: Pacific Northwest, almost
Last seen: 7 years, 1 month
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Kings have such a fat stipe that it is pretty easy to tear one in two and grab a core sample that is totally pristine. Assuming the rest of your conditions are super sterile I bet you won't have any problem. Yes, agar would be safer.
I have cloned grocery store Kings often with great results.
Good luck!
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Zen Peddler


Registered: 06/18/01
Posts: 6,379
Loc: orbit
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I got shot down initially here for suggesting that my two store bought clones of king oysters performed well and were in discernable from the Aloha strain on agar. What I soon realized was that these people believed everything they read about grower genetics being expanded out to near senescence thus a clone will quickly decline or had simply never tried to grow out a market or store bought clone. there is of course no chance that any commercial grower risks yields by heavy expansion of strain genetics. and some commercial growers I know don't use commercial strains any more. I know an agaricus grower who runs a massive operation all using a strain isolated from a clone.  a one generation clone is unlikely to decline significantly if at all but all strains decline and can potentially throw off odd phenotypes. I'm sure this grow will be fine.
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t3chnobily
Strangest


Registered: 02/04/12
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Loc: As Seen In VT
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cloned king
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thiotimoline
Stranger

Registered: 12/01/12
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Loc: Bay Area
Last seen: 6 years, 1 month
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Sweet! bluemeanie, I admit that I severely overgeneralized from my own blobby failure.
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Zen Peddler


Registered: 06/18/01
Posts: 6,379
Loc: orbit
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Haha. No probs.
I'm not a huge oyster fan in general but I love the flavor of the king oyster!
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Queen of Kings
Get on with the Fascination



Registered: 12/11/11
Posts: 1,201
Loc: Pacific Northwest, almost
Last seen: 7 years, 1 month
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Similarly pilfered Kings. Definitely one of my favorite edibles.
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urthtown
meat popsicle



Registered: 10/26/13
Posts: 1,039
Loc: Eastern Canadia
Last seen: 2 years, 4 months
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Hey guys sorry I missed the conversation here - was working on some other myco related projects and my king clones were tucked away in the cupboard for a couple weeks.
Well I lost one tissue to grain transfer and one took off just fine. Interestingly (anecdotally, not scientifically) I did two grain jars at the same time (see OP) and it was the one without the H202 dip that contammed. Could be coincidence, there's no real way to know where the contam came from. Looked like tiny green rough bubbles all over the grains. The cardboard clone is also growing happily if a bit wispy.

I shook the healthy one as it was at 30% colonized and it's recovering now so I think it will be ok to grow out on supplemented sawdust block as planned.
Great to see all the successful grows on grocery store genetics! My shiitake clone seems like it grew for a bit and then just gave up - thinking it may actually be close to senescence as it hasn't contammed, just stopped growing and shrivelled.
-------------------- Cluster Headache sufferer? Cluster Busting Veil Tear GIF Flower Pot Grow GIF Mini Mono Tub GIFS "All mushrooms are edible, but some only once." -- Croatian Proverb
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urthtown
meat popsicle



Registered: 10/26/13
Posts: 1,039
Loc: Eastern Canadia
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Re: King Oyster Clones [Re: urthtown]
#19471440 - 01/25/14 10:47 AM (10 years, 1 month ago) |
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Update!
I added some pasteurized hardwood sawdust to my cardboard clone jar after hydrating it with some coffee and gypsum. Looks happy! My wheat jar is fuzzing up but just slow slow slow. I think my grain might be too dry. I added 3 cc's of sterile water and noticed some improved mycelium growth.

-------------------- Cluster Headache sufferer? Cluster Busting Veil Tear GIF Flower Pot Grow GIF Mini Mono Tub GIFS "All mushrooms are edible, but some only once." -- Croatian Proverb
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GordonWeasel
Gardener


Registered: 03/04/13
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Last seen: 3 years, 9 months
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Re: King Oyster Clones [Re: urthtown]
#19471492 - 01/25/14 11:03 AM (10 years, 1 month ago) |
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I bought some from that same company and started the clones 12 days ago on little pieces of cardboard in petri dishes. They are very slow as you said. I cloned some grocery store grey oysters and they just started to pin today. The price is right anyway.
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urthtown
meat popsicle



Registered: 10/26/13
Posts: 1,039
Loc: Eastern Canadia
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Cool! I'm hoping to get some spores from a fruit on this sawdust to start sectoring a fresh isolate to grow out as a master culture. I have plenty to keep me busy with the isolates I have purchased so this is a fun side project. :reckon: Good luck.
-------------------- Cluster Headache sufferer? Cluster Busting Veil Tear GIF Flower Pot Grow GIF Mini Mono Tub GIFS "All mushrooms are edible, but some only once." -- Croatian Proverb
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afk77
Stranger

Registered: 12/01/13
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Re: King Oyster Clones [Re: urthtown]
#19475187 - 01/26/14 04:16 AM (10 years, 30 days ago) |
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ive never been able to fruit King Oysters, looks fun though.
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urthtown
meat popsicle



Registered: 10/26/13
Posts: 1,039
Loc: Eastern Canadia
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Re: King Oyster Clones [Re: afk77]
#19475605 - 01/26/14 07:29 AM (10 years, 30 days ago) |
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Quote:
afk77 said: ive never been able to fruit King Oysters, looks fun though.
Did you try a cold shock?
-------------------- Cluster Headache sufferer? Cluster Busting Veil Tear GIF Flower Pot Grow GIF Mini Mono Tub GIFS "All mushrooms are edible, but some only once." -- Croatian Proverb
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Zen Peddler


Registered: 06/18/01
Posts: 6,379
Loc: orbit
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Re: King Oyster Clones [Re: urthtown]
#19478781 - 01/26/14 08:32 PM (10 years, 29 days ago) |
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They are tasty!
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urthtown
meat popsicle



Registered: 10/26/13
Posts: 1,039
Loc: Eastern Canadia
Last seen: 2 years, 4 months
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Going to spawn one of these clones this weekend and I'll be prepping my substrate tomorrow.
What do you guys say - shredded straw log or supplemented sawdust block (supplemented with coffee, wheat germ and gypsum)?
Thanks!
-------------------- Cluster Headache sufferer? Cluster Busting Veil Tear GIF Flower Pot Grow GIF Mini Mono Tub GIFS "All mushrooms are edible, but some only once." -- Croatian Proverb
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mycoloco
less and less a stranger



Registered: 06/20/13
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Loc: The Rogue Valley, OR
Last seen: 1 year, 1 month
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Re: King Oyster Clones [Re: urthtown]
#19500854 - 01/31/14 11:27 AM (10 years, 25 days ago) |
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After several frustrating attempts at fruiting kings, I have successfully settled on a mix of 1/3 straw, 2/3 sawdust supplemented with gypsum and finely ground brown rice. I find I get more fruits per pound by growing in jars -- scratch 43 days after inoculation, cold shock for 24 hours, set in pinning/fruiting environment of 55-60 degrees Fahrenheit, 8 hours of light per day.
-------------------- The universe is an illusion of the living.
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Ganzig
It's for the street cred


Registered: 11/29/06
Posts: 8,206
Loc: Oregon
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Re: King Oyster Clones [Re: mycoloco]
#19500876 - 01/31/14 11:33 AM (10 years, 25 days ago) |
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Strange. The kings I have start fruiting before I get a chance to put them in fruiting conditions. Straw, coffee, or whatever. The straw bags and coffee bags I did fruited in my living room with a few sprays from a spray bottle each day.
I had them fruiting off of a few left over grains in the bottom of a jar once as well.
That original culture came from mycopath.com
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I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this. I must keep reminding myself of this.
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