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Offlinemullen1200
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Which of these LC's is most viable?
    #19468263 - 01/24/14 03:48 PM (10 years, 11 months ago)

I know the pictures arent the best, but I took them outside to try to get as clear pictures as I could. I use a maple syrup recipe (and I did it 2+ months ago). I used broken glass as agitators, and shook them once or twice a day for a few weeks at least. For the most part, they've just been sitting in a cabinet. I want to inoculate some BRF jars I'm about to make.

After losing something like 30+ jars to monotub contams I'm going to give this a try for more immediate results.

I gave them a small shake to try to get more to see in the pictures.

Im going to knock up about a dozen jars. I figured it was a much better idea to use my LC (my first attempt by the way), then try something wild like infecting some BRF jars with colonized grains.  Let me know what you experts think! Thanks


1-3

the first one  (farthest on left), appears to have the mycelium "balls" floating around. 2 and 3 are translucent/opaque with very little to see floating around.
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4-6

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7-8
A very solid  "yolk" , and very very black. I figure'd that black was some type of mold, but I have very little experience.
----------------------------------------------------

9-10

The one on the left looks similar, just broken up with the darker color mixed in with it.
The one on the right seems to be one giant cottonball.

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InvisibleFungal growth
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Re: Which of these LC's is most viable? [Re: mullen1200]
    #19468268 - 01/24/14 03:51 PM (10 years, 11 months ago)

Well black is bad, but it is impossible to tell if an LC is clean or not until you knock up a jar.

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OfflineSgt. Pepper
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Re: Which of these LC's is most viable? [Re: mullen1200]
    #19468274 - 01/24/14 03:53 PM (10 years, 11 months ago)

We can never tell you how viable a lc is by looking at it. You'll have to test each one on a grain jar before you use it on a full dozen jars.


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Invisibleblindingleaf
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Re: Which of these LC's is most viable? [Re: mullen1200]
    #19468282 - 01/24/14 03:55 PM (10 years, 11 months ago)

lc are dangerous, and i only see myc in the last two (with 9 also having mold of some sort).  the others are either too thick to see the myc or just not gonna grow.
i would choose none, but if i was bored and just looking for something to do, i'd go with number ten.
8 and 9 are fucked
if ur previous jars and these were inoculated with same syringe, and urs jars were lost, its likely the syringe has contaminated.  order one from a vendor


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

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Offlinemullen1200
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Re: Which of these LC's is most viable? [Re: blindingleaf]
    #19468294 - 01/24/14 03:59 PM (10 years, 11 months ago)

I knocked up the LCS from multiple syringes from a sponsor (halloween special), Admittedly my methods are far from perfect/sterile. My monotubs failed because of low ratios among other things.

i'll definitely be choosing one of them, it wont be any great loss if it doesnt work out.

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Re: Which of these LC's is most viable? [Re: mullen1200]
    #19468314 - 01/24/14 04:03 PM (10 years, 11 months ago)

R u sure the monos didnt fail cuz your methods are far from sterile?

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Invisibleblindingleaf
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Re: Which of these LC's is most viable? [Re: Fungal growth]
    #19468697 - 01/24/14 05:38 PM (10 years, 11 months ago)

i was thinking same thing ^^^^

do u use an SAB?


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

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OfflinePussyFart
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Re: Which of these LC's is most viable? [Re: mullen1200]
    #19468871 - 01/24/14 06:28 PM (10 years, 11 months ago)

Quote:

mullen1200 said:
After losing something like 30+ jars to monotub contams I'm going to give this a try for more immediate results.

LC



:failboat:

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Offlinemullen1200
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Re: Which of these LC's is most viable? [Re: PussyFart]
    #19469071 - 01/24/14 07:08 PM (10 years, 11 months ago)

Quote:

PussyFart said:
Quote:

mullen1200 said:
After losing something like 30+ jars to monotub contams I'm going to give this a try for more immediate results.

LC



:failboat:





Lol thanks, was that necessary?

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Invisibleblindingleaf
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Re: Which of these LC's is most viable? [Re: PussyFart]
    #19469079 - 01/24/14 07:10 PM (10 years, 11 months ago)

lol

lc =  :wrongway:

but in all seriousness OP, like i said, if u are bored and have the extra time/supplies, try it (jar 10 that is)  worst that would happen is u waste 5 minutes of ur life and 1 jar.  I get bored often enough that i would personally try it.

id also spend 5-10 minutes burning 2 arm holes in a clear plastic tote (at LEAST 66qt, try for bigger/taller.  u want 2 quart jars to be able to fit on top of one another ideally)  that u can use for years as an SAB.

i wish u luck!  let us know how it goes!


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

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Offlinevikingsc
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Re: Which of these LC's is most viable? [Re: blindingleaf]
    #19469510 - 01/24/14 08:53 PM (10 years, 11 months ago)

Quote:

blindingleaf said:
lc are dangerous, and i only see myc in the last two (with 9 also having mold of some sort).  the others are either too thick to see the myc or just not gonna grow.
i would choose none, but if i was bored and just looking for something to do, i'd go with number ten.
8 and 9 are fucked
if ur previous jars and these were inoculated with same syringe, and urs jars were lost, its likely the syringe has contaminated.  order one from a vendor



:whathesaid:

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Offlinemullen1200
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Re: Which of these LC's is most viable? [Re: vikingsc]
    #19471706 - 01/25/14 11:57 AM (10 years, 11 months ago)

Multiple syringes, all from a vendor. Jars colonized fine, I was just losing them after spawning to bulk.

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InvisiblebodhisattaMDiscordReddit
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Re: Which of these LC's is most viable? [Re: mullen1200]
    #19471721 - 01/25/14 11:59 AM (10 years, 11 months ago)

Quote:

mullen1200 said:
Multiple syringes, all from a vendor. Jars colonized fine, I was just losing them after spawning to bulk.




if it contams before the first flush the contam was in your spawn jars hence in your LC, or spore syringe if you skipped the LC like you should have.

Lc is just more steps and more time. If you're hell bent on making a LC use an agar wedge to inoculate it.

Edited by Trusted cuItivator (01/25/14 12:02 PM)

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Offlinemullen1200
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Re: Which of these LC's is most viable? [Re: bodhisatta]
    #19473385 - 01/25/14 06:28 PM (10 years, 11 months ago)

Quote:

bodhisatta said:
Quote:

mullen1200 said:
Multiple syringes, all from a vendor. Jars colonized fine, I was just losing them after spawning to bulk.




if it contams before the first flush the contam was in your spawn jars hence in your LC, or spore syringe if you skipped the LC like you should have.

Lc is just more steps and more time. If you're hell bent on making a LC use an agar wedge to inoculate it.




Your really saying its impossible to contam any other way then the jars, if it doesnt make it to first flush?

Improper pasteurization could do the same thing couldnt it. Cold temps stalling growth, combined with a low spawn ratio leaves plenty of room for contams (it wont colonize fast enough).

I just dont see how you can KNOW it was the jars. It seems that unless your in an amazingly sterile environment, there is plenty of time for exposure to contaminants during the spawning phase. If the jars were bad, I sure as hell couldnt tell from the outside. And i've gone through LOTS of them. I was very ambitious at first and made a ton.

Long story short, i've ended up with a shitload of trich in the end. I've learned from a few of my mistakes, which is what this is all about for me.

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InvisiblebodhisattaMDiscordReddit
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Re: Which of these LC's is most viable? [Re: mullen1200]
    #19473644 - 01/25/14 07:26 PM (10 years, 11 months ago)

Most trusted cultivators and everyone else who has ever gotten somewhat far in this hobby will tell you if it contams before the first flush it was your spawn jars.

Improper pasteurization with poo perhaps but with coir no, you can sterilize that shit and be just fine. Its just a bad habit. Cool temps would have actually helped you out.


Quote:

I just dont see how you can KNOW it was the jars.



Quote:

RogerRabbit said:
When they contaminate before first flush, it's nearly 100% guaranteed that it's your spawn that's contaminated.  Pay especial attention to sterile procedure during inoculation.
RR



It's not just RR's sentiment either, this is pretty much everyone experience.
Quote:

FrankHorrigan said:
If your grain spawn is clean and fully colonized and you have properly pasteurized your substrate, you will not see a contam before the first flush.




Quote:

It seems that unless your in an amazingly sterile environment, there is plenty of time for exposure to contaminants during the spawning phase.



Yes, and we expect it. No one spawns in sterile environments unless you're spawning to already sterile media. Coir is sort of an exception you can really botch it up and coir will save your ass. You can spawn over your shitter and sneeze right in there while you're doing it, I don't suggest you do however.

Edited by Trusted cuItivator (01/25/14 07:29 PM)

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