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Invisiblejpack666
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Protecting agar from contams during incubation??
    #19464325 - 01/23/14 07:30 PM (10 years, 11 months ago)

Hi folks,
the subject says it all, almost

after pouring agar into sterile plastic dishes, what do you do with them, how do you protect them from contams?

see I've seen a lot of mycology videos, mostly in front of flow hoods, where they go through the quick pour process into 20 dishes or so and then the video ends...

well I know if they sit there on a counter the agar will solidify in the dish, but the dish gives GE without any filtering... so how can it be protected?

I'm probably missing something obvious...

Thanks for the help

cheers


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Invisiblemushmagic
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Re: Protecting agar from contams during incubation?? [Re: jpack666]
    #19464355 - 01/23/14 07:36 PM (10 years, 11 months ago)

Parafilm or glad wrap which is gas permeable. Pour dishes in a SAB or in front of a flowhood.


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Invisiblejpack666
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Re: Protecting agar from contams during incubation?? [Re: mushmagic]
    #19464512 - 01/23/14 08:05 PM (10 years, 11 months ago)

cool and I'm guessing you just take it out of the box and use it... guessing it's a pretty clean product that doesn't need any sterilization?


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Invisiblemushmagic
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Re: Protecting agar from contams during incubation?? [Re: jpack666]
    #19464527 - 01/23/14 08:08 PM (10 years, 11 months ago)

Yes just don't pull any of it out unless you're in front of a flowhood or inside a SAB. To be safe you could not use the first little bit of the roll.


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OfflineRogerRabbitM
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Re: Protecting agar from contams during incubation?? [Re: jpack666]
    #19464924 - 01/23/14 09:24 PM (10 years, 11 months ago)

Quote:

jpack666 said:


see I've seen a lot of mycology videos, mostly in front of flow hoods, where they go through the quick pour process into 20 dishes or so and then the video ends...





That's because you only watched the free sample clip.  The full video shows it all.  Slip the freshly poured dishes back into the sleeve they came packed in.  Fold it under the bottom so the weight of the dishes keeps it sealed.

Leave like that until inoculation, and then wrap each dish in parafilm.  I slip each dish or set of dishes into brand new ziplock bags for colonization.  Inflate the bags with sterile air from the flowhood before closing.
RR


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Invisiblejpack666
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Re: Protecting agar from contams during incubation?? [Re: RogerRabbit]
    #19466539 - 01/24/14 08:29 AM (10 years, 11 months ago)

wow sounds great thanks everyone. I'll do what mushmagic said about the parafilm, only have SAB, so yeah I'll only use the box when inside the SAB and cut away the start of the roll I guess.

RR I think I saw that part where they put them back in the sleeves, and then only after colonization you put them in parafilm... interesting

What's your angle on this one, does it have practical advantages compared to putting in parafilm as soon as knocked up?

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InvisiblebodhisattaMDiscordReddit
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Re: Protecting agar from contams during incubation?? [Re: jpack666]
    #19466581 - 01/24/14 08:46 AM (10 years, 11 months ago)

I along with a lot of other cube grower here for practical purpose just wrap the dishes with saran wrap. Saran wrap is inferior to parafilm but it will work for your purpose. I cut the roll into 1 inch lengths then have a mini "tape" roll of saran wrap. I use this on my dishes now. It works well. RR might say saran wrap is crap but he also works on a mushroom farm where proper techniques and top of the line things are the norm. For all your purpose you can get away with saran or parafilm right after inoculation. When you want to take a transfer from the dish unwrap it in your SAB take a xfer and then if you're saving the old dish re-wrap it. If you can afford a roll of parafilm go for it though it's nice stuff.

Edited by Trusted cuItivator (01/24/14 08:46 AM)

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Re: Protecting agar from contams during incubation?? [Re: bodhisatta]
    #19466872 - 01/24/14 10:14 AM (10 years, 11 months ago)

Can you use micro pore tape to wrap the petri dishes?


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Re: Protecting agar from contams during incubation?? [Re: 1down5up]
    #19466879 - 01/24/14 10:15 AM (10 years, 11 months ago)

Quote:

1down5up said:
Can you use micro pore tape to wrap the petri dishes?




yes and that's what I was using however it's kind of a pain to work with inside of a SAB and it does leave a sticky residue sometimes on the dishes, I have glass dishes and i would say like 25% of the time it leaves a horrible stick for whatever reason.

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Re: Protecting agar from contams during incubation?? [Re: 1down5up]
    #19466912 - 01/24/14 10:25 AM (10 years, 11 months ago)

:thumbup: Thanks!


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No one is placed higher than another no matter race or creed or gender, we must teach forgiveness and compassion for all life.  J.L.

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Invisiblejpack666
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Re: Protecting agar from contams during incubation?? [Re: bodhisatta]
    #19466944 - 01/24/14 10:38 AM (10 years, 11 months ago)

Quote:

bodhisatta said:
I along with a lot of other cube grower here for practical purpose just wrap the dishes with saran wrap. Saran wrap is inferior to parafilm but it will work for your purpose. I cut the roll into 1 inch lengths then have a mini "tape" roll of saran wrap. I use this on my dishes now. It works well. RR might say saran wrap is crap but he also works on a mushroom farm where proper techniques and top of the line things are the norm. For all your purpose you can get away with saran or parafilm right after inoculation. When you want to take a transfer from the dish unwrap it in your SAB take a xfer and then if you're saving the old dish re-wrap it. If you can afford a roll of parafilm go for it though it's nice stuff.




Thanks! nice home grower idea. the saran wrap tape roll.
Must be a pain for you to succeed with a method that goes without the GE that books talk about. You probably had to take a risk and hope that no GE for your dishes would still work... Props, sounds ideal also for a SAB.

How do you cut it in 1" lines? Do you also do that in the SAB to make sure your saran stays as clean as possible. Do you open the saran box inside the SAB and exit the SAB only once your saran tape roll is in a ziploc?

Having no GE, does it cause extra condensation? Do you store your dishes upside down as a way to avoid contams because of this?

Having no GE, are your dishes good for less time than if you gave them GE, if you have made any experiments with that....

Good stuff, thanks again!

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Re: Protecting agar from contams during incubation?? [Re: jpack666]
    #19466957 - 01/24/14 10:41 AM (10 years, 11 months ago)

I cut the saran wrap on my cutting board in the kitchen. I use a very very very sharp chef knife without serration but a serrated knife might work best if you have average knifes, I can chop the roll with a quick whack. The inside of the roll is protected by the outside of the roll and it doesn't have to be that clean. As for gas exchange the volume of air inside of the dish is sufficient IMO to take care of growth for quite a long time, besides I think the saran wrap by nature will let some gas diffuse through any little gap in the film around the dish. Most people put their dishes into a ziplock so how does that not limit GE, just cus the volume of the baggie is bigger? Either way it works just fine all the time in practice.

you could also probably hack through the saran to make the mini rolls with a razor blade.

Edited by Trusted cuItivator (01/24/14 10:43 AM)

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Invisiblejpack666
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Re: Protecting agar from contams during incubation?? [Re: bodhisatta]
    #19467017 - 01/24/14 10:59 AM (10 years, 11 months ago)

lol, I was completly picturing this wrong, the long way. it just clicked only after I read what you just wrote...

OK, now that I'm on the same track, you're cutting the 1" pieces BEFORE ever unrolling the roll of saran wrap (of course because anyone would know this :wink:)

Good.

The only thing I wonder now is if you end up keeping the center cardboard or if you end up sliding it out and discarding it.

Props for making that AIR VOLUME thing clear, GE is to me, often a scary concept...

And... IF (your words), most people use ziplocs, why not you? is it practicality? cost effectiveness? or better contam protection?

cheers and thanks again

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Re: Protecting agar from contams during incubation?? [Re: jpack666]
    #19467077 - 01/24/14 11:15 AM (10 years, 11 months ago)

Ziplocks are expensive. I put my dishes back into a tote used for mycology stuff it stays clean in there, I've never had a problem that I couldn't trace back to my own shitty hand movements or being way to stoned to do SAB work.

also there's no way in hell you could slide the inner cardboard tube out. You cut that with the roll. The very last piece of saran wrap is nearly glued to the tube. It keeps the small 1 inch roll rigid too.

Putting your poured then wrapped petris in a ziplock is still a good idea though.

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Invisiblemushmagic
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Re: Protecting agar from contams during incubation?? [Re: jpack666]
    #19467386 - 01/24/14 12:26 PM (10 years, 11 months ago)

Quote:

jpack666 said:
Must be a pain for you to succeed with a method that goes without the GE that books talk about. You probably had to take a risk and hope that no GE for your dishes would still work...





Not if you get the right kind of saran wrap. Glad brand saran wrap is actually gas permeable:thumbup:


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Invisiblejpack666
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Re: Protecting agar from contams during incubation?? [Re: mushmagic]
    #19467485 - 01/24/14 12:50 PM (10 years, 11 months ago)

nice, thanks for all the info folks

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