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InvisibleGanzig
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Re: Pestalotiopsis microspora [Re: Alan Rockefeller]
    #19461861 - 01/23/14 10:29 AM (10 years, 3 months ago)

Perhaps it could be used in a localized way for some specific goal but
after considering what you just said Alan, I have to agree with you.

It takes a lot of energy to make plastic(relatively) and Gathering it up
for re-use is probably a much smarter move.

Perhaps they could be used as a cleaner for a head of a plastic 3d printer.

Not harsh chems and no by products. Except Co2 of course.


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OfflineAlan RockefellerM
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Re: Pestalotiopsis microspora [Re: Ganzig]
    #19461883 - 01/23/14 10:37 AM (10 years, 3 months ago)

Quote:

Ganzig said:
Perhaps they could be used as a cleaner for a head of a plastic 3d printer.





That is a creative idea, however I think that heat would be a much better way to clean out the head of a 3d printer.  Waiting weeks for mycelium to colonize instead of just heating it up and sticking a nail in there would be quite a slow and expensive solution for a clogged head. 

And that's the same problem with the rest of mycoremediation.  No one disputes that it works, it's just not a very practical way to solve a problem.  I've yet to see any environmental problem that mushrooms can solve better than more conventional means.  Maybe one day someone will discover one, until that day mycoremediation will be performed only by well meaning but misguided hippies.

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InvisibleGanzig
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Re: Pestalotiopsis microspora [Re: Alan Rockefeller]
    #19461923 - 01/23/14 10:49 AM (10 years, 3 months ago)

Yeah good point.
I am sure there is something it could be used for. Perhaps it's best use
is happening right where it was found. There has got to be something useful
besides its natural role.



I don't know enough about the mycoremediation stuff to comment but I see you point.

Hippies.


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InvisibleWillSolvem
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Re: Pestalotiopsis microspora [Re: Alan Rockefeller]
    #19461956 - 01/23/14 10:56 AM (10 years, 3 months ago)

I think people are going in the wrong direction with mycorestoration.

Instead of trying to grow mushrooms off of trash (whatever it may consist of) I think a better idea would be to setup a bioreactor that would draw off the acid/enzymes, then isolate the responsible enzyme and apply that to landfills. Enzyme sprayers on the back of dump trucks sounds like a practical way of degrading waste that wasn't recycled but still headed to a landfill.

I am currently working on such a method. :thumbup:


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InvisibleGanzig
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Re: Pestalotiopsis microspora [Re: WillSolvem]
    #19461975 - 01/23/14 11:00 AM (10 years, 3 months ago)

I am willing to bet that such enzymes are already being produced.

Are you at a college or university? You have access to a bio reactor, mass spec and the like?


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OfflineAlan RockefellerM
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Re: Pestalotiopsis microspora [Re: WillSolvem]
    #19462152 - 01/23/14 11:51 AM (10 years, 3 months ago)

Quote:

WillSolvem said:
Instead of trying to grow mushrooms off of trash (whatever it may consist of) I think a better idea would be to setup a bioreactor that would draw off the acid/enzymes, then isolate the responsible enzyme and apply that to landfills. Enzyme sprayers on the back of dump trucks sounds like a practical way of degrading waste that wasn't recycled but still headed to a landfill.





That is an interesting idea, but how is it better than just burying the trash?

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Invisiblestevo

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Re: Pestalotiopsis microspora *DELETED* [Re: Ganzig]
    #19462192 - 01/23/14 11:58 AM (10 years, 3 months ago)

Post deleted by stevo

Reason for deletion: .

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OfflineAlan RockefellerM
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Re: Pestalotiopsis microspora [Re: stevo]
    #19462243 - 01/23/14 12:06 PM (10 years, 3 months ago)

I don't think there are any fungi that can eat rubber tires.  But even if there were, I don't think we would want to use them.  If we spend all this time and energy making tires, why throw away the raw materials by composting them?  Seems like it would make more sense to me to use the rubber from old tires to make rubber bands, roads, etc.

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InvisibleGanzig
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Re: Pestalotiopsis microspora [Re: Alan Rockefeller]
    #19462383 - 01/23/14 12:42 PM (10 years, 3 months ago)

Quote:

Alan Rockefeller said:
Quote:

WillSolvem said:
Instead of trying to grow mushrooms off of trash (whatever it may consist of) I think a better idea would be to setup a bioreactor that would draw off the acid/enzymes, then isolate the responsible enzyme and apply that to landfills. Enzyme sprayers on the back of dump trucks sounds like a practical way of degrading waste that wasn't recycled but still headed to a landfill.





That is an interesting idea, but how is it better than just burying the trash?




I think it would be better to have more basic organic materials(which I think is what we are trying to get at by using these types of organism) than say a kids plastic kitchen set underground. Perhaps fewer
off gasses or pollutants seeping into ground water.
That is if you can change enough stuff into organic matter to make a difference.
If it is to sit under ground for years you might as well help the process along.

Nobody is going to dig up buried tires to use them. But I agree with not
destroying them when we can use them for so many things. Shoes, structures, boat bumpers, artificial reefs, etc.


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Invisiblestevo

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Re: Pestalotiopsis microspora *DELETED* [Re: Ganzig]
    #19462501 - 01/23/14 01:09 PM (10 years, 3 months ago)

Post deleted by stevo

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InvisibleWillSolvem
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Re: Pestalotiopsis microspora [Re: Alan Rockefeller]
    #19462733 - 01/23/14 01:58 PM (10 years, 3 months ago)

Quote:

Ganzig said:
I am willing to bet that such enzymes are already being produced.




They are, taken from the article bellow regarding the species pestalotiopsis:
"isolated the enzymes that allow the organism to degrade plastic as its food source."

http://aem.asm.org/content/79/23/7313.abstract


Quote:

Ganzig said:
Are you at a college or university? You have access to a bio reactor, mass spec and the like?




Not at a university but a privately funded laboratory, and yes I have access to the culturing, analytical, AND isolation/refinement equipment needed.

Quote:

Alan Rockefeller said:
I don't think there are any fungi that can eat rubber tires.  But even if there were, I don't think we would want to use them.  If we spend all this time and energy making tires, why throw away the raw materials by composting them?  Seems like it would make more sense to me to use the rubber from old tires to make rubber bands, roads, etc.




If pestalotiopsis can eat polyurethane it can eat ties:

"Polyurethane is also used in making solid tires. Industrial applications include forklift drive and load wheels, grocery cart and, rollercoaster wheels..."

http://en.wikipedia.org/wiki/List_of_polyurethane_applications


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OfflineAlan RockefellerM
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Re: Pestalotiopsis microspora [Re: stevo]
    #19462896 - 01/23/14 02:38 PM (10 years, 3 months ago)

Quote:

stevo said:
It would be more practical for smaller operations I think. They just pile them up at the dump dont they?  Recycling takes start up funds.  Do you really think it'd be easier to turn it into fuel oil or rubber bands than pour basically a big lc on the pile?  I mean I know the lc would cost some money.





That is a good thought, but growing fungi is usually a lot more difficult than just throwing LC at stuff.    I do not think there are any fungi that eat rubber, perhaps there are some bacteria that would do something.....but it would take forever....My thoughts on recycling tires is that if it is not economically viable to recycle them, we should just bury them. 

Quote:

Ganzig said:
I think it would be better to have more basic organic materials(which I think is what we are trying to get at by using these types of organism) than say a kids plastic kitchen set underground. Perhaps fewer
off gasses or pollutants seeping into ground water.




I would like to give this Pestalotiopsis microspora a try, but I was thinking the opposite - that if we are breaking down plastics into organic and water soluble molecules, that could release a lot of toxins, dyes, etc into the groundwater.  And that we would be better off having all of those molecules locked up in the polymers.

Quote:

That is if you can change enough stuff into organic matter to make a difference.
If it is to sit under ground for years you might as well help the process along.




If it is going to sit in the ground for years, why help the process along?  Why not just leave it?  It's not like we are going to dig up the landfills later for some reason - They will always be very junky, debris filled places.  There will always be a lot of things that just don't break down, that is the nature of garbage.  Does it really matter at the end of the day (or 100 years) if 40% or 60% or 70% of the garbage breaks down?


Quote:

stevo said:
Money shouldn't be the only virtue.  The easiest way to clean up a big mess is to throw it all in a pile and light a match but that is just ignorant to nature.




Money should be a pretty big consideration - If we are going to be spending peoples tax money / garbage bill money, it needs to be spent wisely.  People are not going to pay their hard earned money on novel efforts which don't make much difference in the long run anyway. 

Quote:

For example I know where there is lots of trash piles.  It's not even my property or business.  It does bother me but not so much that I'm willing to risk going to jail for stealing toilet seats and coke bottles out of the a state forest just to pay to have it thrown away in a landfill at my expense.  But shit if there was only a live plasticBgone whether commercial or home cultured I'm down.  The flaws I see in recycling and science in general are in the organizations themselves and those who care more about driving a porsche or 3 than any principle.





It is very cool that you want to clean up the trash, but if you are going to spend your time on it, you need to be smart about it.  Even if there is a cool fungus that breaks down one or two types of plastic, that would be a very small percentage of the total garbage pile.  It might even look junkier if the plastic started to break down and little bits blew all over in the wind.  I think the best thing to do would be to organize a work party, and ask the local forest managers if you can get funding for a dump run.   

One thing someone told me recently, and I am not sure if it is true, is that recently they started using biodegradable plastic bags in the stores.  Before they did this, if someone littered a bag, it would be visible, but would eventually get cleaned up whole.  Now the bags turn into plastic dust and they can't really be cleaned up anymore.  And microscopic plastic dust is blowing around everywhere and maybe not so good for the environment or peoples health.  I don't know if this is true, but it's something to consider - We may not actually want everything to break down.  Might be better for the earth if it doesn't.

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InvisibleGanzig
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Re: Pestalotiopsis microspora [Re: Alan Rockefeller]
    #19462944 - 01/23/14 02:47 PM (10 years, 3 months ago)

Again really good point.

Much research is needed to answer these great questions that we have come up with.


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Invisiblestevo

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Re: Pestalotiopsis microspora *DELETED* [Re: Ganzig]
    #19464466 - 01/23/14 07:54 PM (10 years, 3 months ago)

Post deleted by stevo

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InvisibleVersicolor
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Re: Pestalotiopsis microspora [Re: stevo]
    #19465344 - 01/23/14 10:56 PM (10 years, 3 months ago)

Quote:

stevo said:
burn some tires and contain the smoke in a water filter like a big water bong and mix the collected smoke sludge with substrate and let microbes do the work you would not need this fungus then you spread the mushrooms out?




:wellholyshit:

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InvisibleAmphibolos
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Re: Pestalotiopsis microspora [Re: Alan Rockefeller]
    #19468244 - 01/24/14 03:42 PM (10 years, 3 months ago)

Quote:

If it is going to sit in the ground for years, why help the process along?  Why not just leave it?  It's not like we are going to dig up the landfills later for some reason - They will always be very junky, debris filled places.  There will always be a lot of things that just don't break down, that is the nature of garbage.  Does it really matter at the end of the day (or 100 years) if 40% or 60% or 70% of the garbage breaks down?




I think that we could help speed the process by actually designing the landfills, instead of just burying the trash. Like creating the hole and coating the sides of it with recycled concrete or something cheap to avoid toxic leaks. We then put an hole in the center to redirect the water. That way we can stock the water and recycle the rare metals with catalysts


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Re: Pestalotiopsis microspora [Re: Amphibolos]
    #19468768 - 01/24/14 06:01 PM (10 years, 3 months ago)

Thank you eppendorf!

Who wants bioreactor porn?!



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InvisibleGanzig
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Re: Pestalotiopsis microspora [Re: WillSolvem]
    #19468786 - 01/24/14 06:06 PM (10 years, 3 months ago)

:Awemazing:


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InvisibleWillSolvem
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Re: Pestalotiopsis microspora [Re: Ganzig]
    #19468989 - 01/24/14 06:52 PM (10 years, 3 months ago)

I'll post more tomorrow after its put together :thumbup:


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Offlinegrof
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Re: Pestalotiopsis microspora [Re: WillSolvem]
    #20746105 - 10/24/14 05:31 AM (9 years, 6 months ago)

anything news?

has anybody here got it to get and grow Pestalotiopsis microspora?

Best whishes

Edited by grof (10/24/14 05:31 AM)

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