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OfflineTabensis
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Re: First Grow (Log kinda) [Re: NitroCat]
    #19441322 - 01/19/14 06:35 AM (10 years, 1 month ago)

The last jar looks overly wet, not so much contaminated but it'll head that direction for sure.


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OfflineNitroCat
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Re: First Grow (Log kinda) [Re: Tabensis]
    #19441389 - 01/19/14 07:10 AM (10 years, 1 month ago)

Yeah, I'd already called myself on that one. I was using entirely too much water, and I'd had finer verm than I'd like, new ones were all done in a coarser one with a fair bit less water, will try and snag a pic or two before innoc tomorrow!


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OfflineBillNyeScienceGuy
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Re: First Grow (Log kinda) [Re: NitroCat]
    #19441630 - 01/19/14 09:01 AM (10 years, 1 month ago)

I'm new here, but I have been lurking a while and I am not sure what tek your using? Because your preparing your jars like WBS or RYE and you are doing BRF cakes........ You will have success following this tek to the point. http://www.shroomery.org/forums/showflat.php/Number/11585613#11585613
check the video out.. http://www.mushroomvideos.com/Terrarium-Tek
(this ones outdated but a little more in depth for you.. http://www.fungifun.org/English/Pftek)
layer of verm on top of your brf cake is what protects it from contams and what not, the wholes on the top should not be covered.  The last picture you posted looked like it would end up contaminated my advice throw out now
Just trying to help you save some time, and reduce or contam rates, those bigger jars are more prone to contam especially when you are not using a top verm layer.


Edited by BillNyeScienceGuy (01/19/14 09:04 AM)


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Offlineslh980
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Re: First Grow (Log kinda) [Re: NitroCat]
    #19441643 - 01/19/14 09:05 AM (10 years, 1 month ago)

Quote:

NitroCat said:






That looks contaminated with multiple molds/bacteria....


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OfflineNitroCat
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Re: First Grow (Log kinda) [Re: BillNyeScienceGuy]
    #19441663 - 01/19/14 09:13 AM (10 years, 1 month ago)

I've actually read over each and every one of those links a few times now, but they're admittedly where a lot of my thinking is coming from. I'm a fairly "Learn by doing" person, and figure even a massively cocked up cake is less likely to kill me than some of the other shit I used to play with..

As to the dry verm and that one jar..
For the filter normally provided by verm I'm using these http://www.shroomery.org/forums/showflat.php/Number/10628004 or something roughly based off of it. And the jar is isolated from the rest at the moment, the black spots while disturbing have at the very least not growing in number.. And the growth is otherwise looking rather positive. I'll honestly be happy if I get anything out of any of the jars in that batch; They're all massively over hydrated, using fine verm, and quite a few got jostled to the point of getting packed at one point or another. (Steaming in unstable boilers was a biiig contributor)
Running a nice, big PC now which is doing wonders for the overhydration and compacting issues.


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OfflineNitroCat
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Re: First Grow (Log kinda) [Re: slh980]
    #19441672 - 01/19/14 09:15 AM (10 years, 1 month ago)

I only counted one (very) possible mold (What I'd been discussing as possible black-pin on page 2), but do see a few things that could be bacterial.. Sadly I've seen the possible bacteria specks on a few of the jars down with that syringe.


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OfflineTabensis
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Re: First Grow (Log kinda) [Re: NitroCat]
    #19441684 - 01/19/14 09:19 AM (10 years, 1 month ago)

Something I found helped me was mixing the water and vermiculite first before adding your flour, it makes for a much more consistent substrate.


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OfflineNitroCat
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Re: First Grow (Log kinda) [Re: Tabensis]
    #19441906 - 01/19/14 10:28 AM (10 years, 1 month ago)

Was already doing that! ^^ The batches made using the coarser verm look much, much, much nicer on the hydration front. 12 of those in the PC waiting for me to go and innoc them, might go do that after another cup of coffee


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OfflineNitroCat
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Re: First Grow (Log kinda) [Re: NitroCat]
    #19443121 - 01/19/14 03:15 PM (10 years, 1 month ago)

Well, sorry to bump this up again but I found out a major cause of my issues... I'm not exactly sure -how- yet, but every jar from the last batch had been massively penetrated by water. Filters soaked, cakes a solid puck of over hydrated verm and flour.

My personal theory is I'm fucking up the foil. No jars survived this round and there had been a massive effort to standardize the foil seals, on the plus side I guess that showed me I still have a problem neatly sealing foil against something. Jumping to my foiling because in this last batch's case, the jars were barely in direct contact with the water (About a half inch or so at the most), double stacked with each lid being ruined and the substrate FAR wetter than I'd initially put it in.

If anyone has a link to a foiling tek, I'll gladdly take it, otherwise going to be looking at that part of other teks and seeing if I can spot my fuckup.

Any other ideas or suggestions greatly appreciated.

*Edit*

Thinking on it, it might well be my innoc ports or the like being too spiky and shoving the foil out of place and or tearing it when I attempt to tighten it down >_>


Edited by NitroCat (01/19/14 04:14 PM)


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OfflinecronicrFacebook
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Re: First Grow (Log kinda) [Re: NitroCat]
    #19443522 - 01/19/14 04:32 PM (10 years, 1 month ago)

yep the ports will do it, but also make sure your not boiling too hard, a nice slow rolling boil is all that is needed


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I'm tired do me a favor


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OfflineNitroCat
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Re: First Grow (Log kinda) [Re: cronicr]
    #19443742 - 01/19/14 05:37 PM (10 years, 1 month ago)

That is a possibility. The last batch was spaced heavily from the bottom using spare rings under the rack though, I'd have thought that'd make a rolling boil less of a issue, also wouldn't the bubble formation in a pressure cooker be smaller anyway? Or am I utterly fucking up my inhead thermo and fluid dynamics


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OfflinecronicrFacebook
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Re: First Grow (Log kinda) [Re: NitroCat]
    #19443748 - 01/19/14 05:39 PM (10 years, 1 month ago)

if you pc'd then it's your lids tearing the foil, try micropore tape


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineNitroCat
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Re: First Grow (Log kinda) [Re: cronicr]
    #19443769 - 01/19/14 05:46 PM (10 years, 1 month ago)

Micropore can just be put over the filter and it'd be protected? Or did I misread that?


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OfflinecronicrFacebook
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Re: First Grow (Log kinda) [Re: NitroCat]
    #19443772 - 01/19/14 05:47 PM (10 years, 1 month ago)

yep micrpore tape your injection holes and put your foil on:thumbup:


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineNitroCat
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Re: First Grow (Log kinda) [Re: NitroCat]
    #19443792 - 01/19/14 05:53 PM (10 years, 1 month ago)

I'll give it a try :laugh: Will be a proper pain to rip the old ports out D:


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OfflineTabensis
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Re: First Grow (Log kinda) [Re: NitroCat]
    #19448120 - 01/20/14 02:58 PM (10 years, 1 month ago)

Add less water to your PC or raise your jars, but never should your jars be sitting directly on the bottom.


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OfflineNitroCat
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Re: First Grow (Log kinda) [Re: Tabensis]
    #19448761 - 01/20/14 05:28 PM (10 years, 1 month ago)

They never have! I added three quarts, the water level suggested by the PC for a cycle. The rack was elevated on spare rings to point the waterline barely came up the the base of the jars. Looking at my foils compared to a few others, it was possibly catching water and encouraging condensation on the bottom of the foil while not letting gasses escape sufficiently and thus causing the filter to just turn into a water-pass.

Trying a different approach to it, PC is going to be cracked for inoculation after work tonight ^,,^
8 BRF Jars, threw in 8 pint jars of Spelt/Millet (Mostly spelt) as well because it was the closest either store I stopped by seemed to have to straight up rye grain.

*edit*
Typos and spelling :V


Edited by NitroCat (01/20/14 05:36 PM)


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OfflineNitroCat
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Re: First Grow (Log kinda) [Re: NitroCat]
    #19453727 - 01/21/14 04:40 PM (10 years, 1 month ago)

Well, sadly the last PC cycle and pile of supplies were lost to my idea for foils being the exact, utter opposite of right. I thought they were too lose, then I saw pics of stuff like Frank PCing jars of grain with exposed polyfil and went "...welp" seeing as mine were already in the cooker.

Ah well, lesson learned! And here's the pics for troubleshooting's sake.


Cake mix! :V



Lids, assembled, dry and clean D:



And then... Disappointment (If someone tells me there were fine I'm going to be so pissed at myself for trashing em)




Broken cake, to show texture D:



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OfflineBillNyeScienceGuy
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Re: First Grow (Log kinda) [Re: NitroCat]
    #19457068 - 01/22/14 11:00 AM (10 years, 1 month ago)

your lids simply need holes in them. not all that extra, it needs to breathe during colonization... your cake looks fine just follow the TEK there's no need to break your cake up to show us just shoot that jar up and be patient, don't tip the jars upside down or look at them every day. Also is that your still air box? You don't need one really, but if your trying to G2G and such look up and create one its very simple and will decrease the contam rates.


Edited by BillNyeScienceGuy (01/22/14 11:04 AM)


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OfflineNitroCat
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Re: First Grow (Log kinda) [Re: BillNyeScienceGuy]
    #19457753 - 01/22/14 02:06 PM (10 years, 1 month ago)

I broke them because everything had water penetration, unless you're saying wet tyvek is fine for a filter? Those were all tossed before being inoculated with anything, so cost essentially nothing. And no, it's not a SAB, it will be whenever I get a hole saw though.

The initial cakes are being left alone at this point aside from the occasional photo and are looking good :laugh: (Aside from being way too damned wet). I guess "first grow" is a bit of a misnomer with this topic :p


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