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maynardjameskeenan
The white stipes



Registered: 11/11/10
Posts: 16,391
Loc: 'Merica
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piximetre manual in english?
#19455539 - 01/21/14 11:46 PM (10 years, 8 days ago) |
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I was wonder if anyone here knew where I could find the instruction manual to piximetre in English? I'm trying to calibrate the program and it keeps coming up with an error message, when I try to figure out how the fix the problem the only instructions it gives me are in french, I've tried to use Google translate to figure out what the page says but firefox tells me it's not a valid URL. I not sure what to do at this point, if anyone can help me I'd greatly appreciate it.
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Gravija
Make way for the cavalcade


Registered: 06/28/11
Posts: 9,063
Loc: Chicago, Illinois, USA
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I used a reticle to measure spores the other day and it didn't give me any error messages If you post the page here I will try to translate it.
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maynardjameskeenan
The white stipes



Registered: 11/11/10
Posts: 16,391
Loc: 'Merica
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Re: piximetre manual in english? [Re: Gravija]
#19458279 - 01/22/14 03:58 PM (10 years, 7 days ago) |
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Thanks for your help and sorry it took me so long to respond, my wife distracts me from important things that I need to get done. I think I get how to calibrate it kinda but it keeps giving me an error message saying something about the image compression is wrong, I'm using the Photobooth program for windows to take pictures but it's only give me one option for the format of the pictures. I'll play with it a little more tonight. Can I set it up for a macro type thing in piximetre so I don't have to calibrate it every time I change magnifications? When I click the 'I' icon for help the instructions it brings up are in French.
-------------------- May you be filled with loving kindness. May you be well. May you be peaceful and at ease. May you be happy. AMU Q&A
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maynardjameskeenan
The white stipes



Registered: 11/11/10
Posts: 16,391
Loc: 'Merica
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This is a screenshot of said error message.
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maynardjameskeenan
The white stipes



Registered: 11/11/10
Posts: 16,391
Loc: 'Merica
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I think I got it figured out. In the 'Compute dimensions window' you click on the 'Parameters tab', go down to 'Images' and change it from 'Normal' to 'Compressed'. A warning box pops up and tells you it wont work unless all the images are the same resolution which mine will be because I'm using the same camera and program to take the micrographs.
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maynardjameskeenan
The white stipes



Registered: 11/11/10
Posts: 16,391
Loc: 'Merica
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I think that I may run into some problem when I try to set the standard for 100X magnification.
 This is .07mm(the smallest division on my micrometer) and it take up 60% of the image, so if I look at it under 100X I wont be able to get the whole object in view, and if I can't see I sure the hell can't measure it.
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maynardjameskeenan
The white stipes



Registered: 11/11/10
Posts: 16,391
Loc: 'Merica
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Here are some rough measurements.

Here are the numbers. I still need to convert whatever .07mm into µm...
' 0.1 [0.11 ; 0.1] x [0.1 ; 0.08] 0.1 .07mm ' Q = 1.2 [1.4 ; 1.6] 1.8 ; N = 10 ; C = 95% ' Me = 0.1 x 0.1 .07mm ; Qe = 1.5
0.12 0.08 0.11 0.07 0.11 0.07 0.11 0.08 0.11 0.08 0.11 0.07 0.11 0.07 0.11 0.07 0.10 0.08 0.12 0.07
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Edited by maynardjameskeenan (01/23/14 12:29 AM)
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TimmiT


Registered: 03/23/10
Posts: 5,303
Loc: Victoria
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Quote:
maynardjameskeenan said: Here are the numbers. I still need to convert whatever .07mm into µm...
To convert mm to µm your just need to move the decimal 3 places to the right (the beauty of the metric system)... so 0.07mm = 70µm. I think your measurements are out by a factor of 10.
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Joust
Mycotographer




Registered: 10/13/11
Posts: 13,392
Loc: WA
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Quote:
maynardjameskeenan said: Here are some rough measurements.

Here are the numbers. I still need to convert whatever .07mm into µm...
' 0.1 [0.11 ; 0.1] x [0.1 ; 0.08] 0.1 .07mm ' Q = 1.2 [1.4 ; 1.6] 1.8 ; N = 10 ; C = 95% ' Me = 0.1 x 0.1 .07mm ; Qe = 1.5
0.12 0.08 0.11 0.07 0.11 0.07 0.11 0.08 0.11 0.08 0.11 0.07 0.11 0.07 0.11 0.07 0.10 0.08 0.12 0.07
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maynardjameskeenan
The white stipes



Registered: 11/11/10
Posts: 16,391
Loc: 'Merica
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Re: piximetre manual in english? [Re: Joust]
#19460722 - 01/23/14 01:51 AM (10 years, 7 days ago) |
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If you you could send me and image at 640x480 of an object of known size I might be able to use it to set a the 100X magnification standard. Maybe something close to .05mm(50µm) in size.
-------------------- May you be filled with loving kindness. May you be well. May you be peaceful and at ease. May you be happy. AMU Q&A
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Alan Rockefeller
Mycologist

Registered: 03/10/07
Posts: 48,271
Last seen: 9 hours, 7 minutes
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Quote:
maynardjameskeenan said: If you you could send me and image at 640x480 of an object of known size I might be able to use it to set a the 100X magnification standard. Maybe something close to .05mm(50µm) in size.
That isn't going to work unless you have the same scope, and even then it would be best to calibrate each scope separately with known reference standards.
You may not be able to use a 70 micrometer hole to calibrate your objective, but you can use it to measure some smaller thing like a spore with the 40x, and then switch to 100x and calibrate using the size of the other thing you measured.
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maynardjameskeenan
The white stipes



Registered: 11/11/10
Posts: 16,391
Loc: 'Merica
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The numbers it give me when I measure the spore are of a percentage of the scale... correct? ie. if it says .11(x-axis) .07(y-axis) it's really means 11%(x-axis) and 7%(y-axis) of 70µm? How can I make it so it gives me the length in whole µm? I'm guessing I need to measure something that is .001(1µm) or .01mm(10µm)?
-------------------- May you be filled with loving kindness. May you be well. May you be peaceful and at ease. May you be happy. AMU Q&A
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maynardjameskeenan
The white stipes



Registered: 11/11/10
Posts: 16,391
Loc: 'Merica
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After playing around with it a little bit and running the number through a calculator The size of those weird Psilocybe/Deconica are roughly 7.7x5.6µm which sorta fits the size of P. silvatica... Don't take my word for it, I'm a total noob. People who know what they're talking about will have legitimate numbers soon.
-------------------- May you be filled with loving kindness. May you be well. May you be peaceful and at ease. May you be happy. AMU Q&A
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Alan Rockefeller
Mycologist

Registered: 03/10/07
Posts: 48,271
Last seen: 9 hours, 7 minutes
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Quote:
maynardjameskeenan said: The numbers it give me when I measure the spore are of a percentage of the scale... correct? ie. if it says .11(x-axis) .07(y-axis) it's really means 11%(x-axis) and 7%(y-axis) of 70µm? How can I make it so it gives me the length in whole µm? I'm guessing I need to measure something that is .001(1µm) or .01mm(10µm)?
No it shouldn't be like that. Your calibration is seriously off. It should be spitting out the number of micrometers, not the ridiculously low numbers that you are getting.
Throw some blood on there and measure that for a sanity check, I have heard that red blood cells should be around 8 but the last time I measured them I found mine were more like 7.4.
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TimmiT


Registered: 03/23/10
Posts: 5,303
Loc: Victoria
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Quote:
Alan Rockefeller said: Throw some blood on there and measure that for a sanity check, I have heard that red blood cells should be around 8 but the last time I measured them I found mine were more like 7.4.
Something in the 6-8µm range is normal. Various conditions will affect the size of erythrocytes though, eg iron deficiency will cause a microcytic (small cell size) anaemia whereas a b12 deficiency will cause a macrocytic (large cell size) anaemia.
-------------------- "Reality leaves a lot to the imagination" ~ John Lennon
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maynardjameskeenan
The white stipes



Registered: 11/11/10
Posts: 16,391
Loc: 'Merica
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Re: piximetre manual in english? [Re: TimmiT]
#19464050 - 01/23/14 06:33 PM (10 years, 6 days ago) |
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Accorded to the standard I set with the .07mm object my wife's red blood cell is 7.7µm.
-------------------- May you be filled with loving kindness. May you be well. May you be peaceful and at ease. May you be happy. AMU Q&A
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Alan Rockefeller
Mycologist

Registered: 03/10/07
Posts: 48,271
Last seen: 9 hours, 7 minutes
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Quote:
TimmiT said: Something in the 6-8µm range is normal. Various conditions will affect the size of erythrocytes though, eg iron deficiency will cause a microcytic (small cell size) anaemia whereas a b12 deficiency will cause a macrocytic (large cell size) anaemia.
Thanks, I googled what you said and found http://en.wikipedia.org/wiki/Red_blood_cell_distribution_width
I am looking for a thing that is really small, really cheap, and always the same size so I can mail people free stage micrometers. Any ideas??? The worlds smallest surface mount component is 203 micrometers wide, and that would work fine for calibrating 400x, but is too large for calibrating 1000x. I am thinking some kind of electronic component would be good, but finding something that is both cheap and small is proving difficult.
It would be even better if we could figure out something that people would have laying around the house, or be able to easily buy at the store, but that's not likely. Currently the cheapest stage micrometers are $13 on ebay and come from china, I would like to get the cost down to around 25 cents.
I have considered cannabis pollen, but anything organic would be variable in size. I think a manufactured product would be more consistent. I know there has to be something that is super cheap and has predictably sized lines....just can't figure out what it would be....
I tried etching glass with a laser and that totally did not work, the cracks were far too unpredictable.
Quote:
maynardjameskeenan said: Accorded to the standard I set with the .07mm object my wife's red blood cell is 7.7µm.
Ok maybe I did not understand your numbers properly.
My numbers look more like this:
6.7 [7.4 ; 7.7] 8.4 × 5.1 [5.5 ; 5.8] 6.3 µm Q = 1.2 [1.3 ; 1.4] 1.5 ; N = 28 ; C = 95% Me = 7.6 × 5.7 µm ; Qe = 1.3
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The Lightning
Mycology Enthusiast


Registered: 09/06/11
Posts: 3,889
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maynardjameskeenan
The white stipes



Registered: 11/11/10
Posts: 16,391
Loc: 'Merica
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8.07 5.19 7.97 5.00 7.85 4.98 7.77 5.19 7.36 5.21 7.82 5.03 7.70 5.46 8.00 4.96 7.77 5.27 7.74 5.12
7.4 [7.7 ; 7.9] 8.2 x 4.8 [5 ; 5.2] 5.4 µm Q = 1.4 [1.5 ; 1.6] 1.7 ; N = 10 ; C = 95% Me = 7.8 x 5.1 µm ; Qe = 1.5

I fixed it! I apologize my ignorance. 
these are the spore sizes for this; http://mushroomobserver.org/149287?q=1l45V
-------------------- May you be filled with loving kindness. May you be well. May you be peaceful and at ease. May you be happy. AMU Q&A
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Alan Rockefeller
Mycologist

Registered: 03/10/07
Posts: 48,271
Last seen: 9 hours, 7 minutes
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The numbers look good!
Regarding the photo, it's a bit grainy. If the table is still you can turn down the ISO and get better quality images.
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