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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Spores "germinate" in pure H2O ?
#18726857 - 08/19/13 10:13 AM (10 years, 6 months ago) |
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I had PCed one of the short jelly jars with a self heal port and double tyvek filter patch so it wouldn't get wet when tipped on its side. This jar was made so I could take a MS syringe and turn it into about 5 more syringes since I have found most vendors syringes are concentrated enough to expand reliably. This particular jar has about 20CC of sterilized h2o with some spores from the original MS syringe in it. Oddly enough inspection shows mycelium about the size of small snow flakes probably 5-10 of them floating around in the clear water. How come spores don't sporulate/germinate in the syringe but they did in distilled PCed water. The syringes I made from expanding it all were viable and produced no contams in the test jars I inoculated. It's like a LC without the syrup. I would attach a picture but there is no way my camera can resolve well enough to see these little snowflakes through the glass.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Spores "germinate" in pure H2O ? [Re: bodhisatta]
#18732007 - 08/20/13 12:19 PM (10 years, 6 months ago) |
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No one has seen myc form in pure h2o with spores before?
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,338
Loc: Dept of know what I'm say...
Last seen: 17 hours, 38 minutes
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Re: Spores "germinate" in pure H2O ? [Re: bodhisatta]
#18732039 - 08/20/13 12:27 PM (10 years, 6 months ago) |
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I have had spores germinate in a syringe. I have actually gotten them from a vendor with little white clumps. I had to shake the hell out of that bitch. I think storing in the fridge helps
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Spores "germinate" in pure H2O ? [Re: bodhisatta]
#18732076 - 08/20/13 12:35 PM (10 years, 6 months ago) |
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Quote:
bodhisatta said: No one has seen myc form in pure h2o with spores before?
It can happen
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Spores "germinate" in pure H2O ? [Re: FrankHorrigan]
#18732100 - 08/20/13 12:39 PM (10 years, 6 months ago) |
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Interesting, good to know. I usually use up all the spore solution I make but these jars were sitting with the colonizing jars for a week or so and it looks like a very clear LC now. I thought for sure I messed up something big time making those jars ha.
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,338
Loc: Dept of know what I'm say...
Last seen: 17 hours, 38 minutes
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Re: Spores "germinate" in pure H2O ? [Re: bodhisatta]
#18732113 - 08/20/13 12:41 PM (10 years, 6 months ago) |
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Well store in the fridge and I bet when you do go to use it that you will see growth very quickly
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PNWmushroomexpert
Stranger


Registered: 12/17/13
Posts: 600
Last seen: 4 years, 3 months
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Re: Spores "germinate" in pure H2O ? [Re: bodhisatta]
#19450062 - 01/20/14 09:51 PM (10 years, 1 month ago) |
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Iam trying to get an answer to this question in my thread. I just got a syringe from a vendor and it had. Myc in it ????
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Quote:
PNWmushroomexpert said: Iam trying to get an answer to this question in my thread. I just got a syringe from a vendor and it had. Myc in it ????
The answer is here, And yes I have seen myc in a "spore syringe" idk just shhh about it I suppose.
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PNWmushroomexpert
Stranger


Registered: 12/17/13
Posts: 600
Last seen: 4 years, 3 months
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Re: Spores "germinate" in pure H2O ? [Re: bodhisatta]
#19450123 - 01/20/14 10:03 PM (10 years, 1 month ago) |
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Ya for sure i aint about to drop names but how cool. Growing never fails to amaze me.
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Spores will sometimes germinate using the nutrients in the water alone...I used distilled water for my syringes and store them at 40F to prevent this.
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