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OfflineKnownPown
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Registered: 10/27/12
Posts: 219
Loc: Washington State
Last seen: 6 years, 6 months
Starting from spores on agar...? Pro's advice welcomed!
    #19401022 - 01/10/14 08:29 PM (10 years, 1 month ago)

Hey everyone I prepared some petri dishes with some PDA agar. The bottle of premix agar I bought says to use 40g/l so for 10 plates I used 250 ml distilled water to 10g of the mix. I did 4 spore inoculations with a B+ print: (will get the other pictures later)





And then I took 5 clones, 4 of which I probably wont open because they look pretty bad, but you tell me =/






The only thing I think I would transfer off these clones is in the first picture, first clone. The print to agar looks pretty good and clean right now too, could I just put a wedge of that to grains once it grows out a little more? Do a few more tranfers?

I have nothing going right now :frown: And would really like to get some grains/tubs going again... I just needed something clean since my last tubs went bad to bacteria/trich...

All input is appreciated, and I'll keep the OP updated as my transfers take off. Planning on doing some work tomorrow after tonights feedback =)


Edited by KnownPown (01/20/14 07:55 PM)


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OfflineCMOS
Whats next?


Registered: 01/08/12
Posts: 833
Last seen: 9 years, 9 months
Re: My growth on agar, with pictures =) [Re: KnownPown]
    #19401190 - 01/10/14 09:06 PM (10 years, 1 month ago)

Quote:

The print to agar looks pretty good and clean right now too, could I just put a wedge of that to grains once it grows out a little more? Do a few more tranfers?





I will usually do one or two transfers from the original print, just to be sure its clean.  Its kinda a clusterfuck with the first plate because of all the different cultures growing.

Could probably get something usable from some of those clone plates, just make sure your opening them in a still air box so anything sporating isn't blowing all over. :thumbup:


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OfflineKnownPown
Fathead Shroomer
Male


Registered: 10/27/12
Posts: 219
Loc: Washington State
Last seen: 6 years, 6 months
Re: My growth on agar, with pictures =) [Re: CMOS]
    #19403203 - 01/11/14 10:51 AM (10 years, 1 month ago)

Yeah that first clone picture looks decent, but I dunno about the rest, they look kinda ran by contamination... I'll do a few transfers today though and hopefully get some clean growth (:


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OfflineKnownPown
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Male


Registered: 10/27/12
Posts: 219
Loc: Washington State
Last seen: 6 years, 6 months
Re: My growth on agar, with pictures =) [Re: KnownPown]
    #19449466 - 01/20/14 07:53 PM (10 years, 1 month ago)

Here's some of my transfers grown out, taken from the B+ spores. Some have nice looking sectors already, and others are lighter/more cottony. Probably take a transfer off of each, and further isolate...










I'd really like to put some of this clean growth to grains asap...Should I let them grow out a little more before taking transfers for grain masters? Should I just wait til I do another transfer and let that grow out before putting to grains?

Also should I be transferring from these by taking the very leading edges of the mycelium that looks more sectored or more cottony?


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OfflineRogerRabbitM
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Registered: 03/26/03
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Last seen: 1 year, 4 days
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Re: My growth on agar, with pictures =) [Re: KnownPown]
    #19449769 - 01/20/14 08:55 PM (10 years, 1 month ago)

Take smaller samples next time you transfer.  Grab mycelium from the leading edge of the growth and try to get a piece no larger than a grain of rice.  This will cut down on the number of strains you get each time.
RR


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