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Offlinepsylosymonreturns
aka Gym Sporrison
Male


Registered: 10/16/09
Posts: 13,948
Loc: Mos Eisley,
Last seen: 3 years, 6 months
Re: Psilocybe silvatica [Re: Anglerfish]
    #19403082 - 01/11/14 10:23 AM (10 years, 1 month ago)

Stipe fibrils easily wash off and are removed with handling . It's not H capnoides or fasiculare so I just guessed the more likely Hyphloma , there's only so many in the PNW.


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InvisibleRuralAnomaly
Sporadic
Male User Gallery

Registered: 10/05/13
Posts: 2,153
Loc: Spitzenkörper Ohio
Re: Psilocybe silvatica [Re: The Lightning]
    #19405001 - 01/11/14 05:58 PM (10 years, 1 month ago)

Quote:

The Lightning said:
I created this for my friends. I consider you one of them.




helluva page!!

i found it a couple months ago all on my ow...google

pleased to be able to compliment the author of it!  the effort is to be really commended.

was there ever a sticky thread of 'great links' for this forum?  if so, this should be included, if not... a good place to start one.
a subsection for id keys would be sweet too, just sayin


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InvisibleThe Lightning
Mycology Enthusiast
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Registered: 09/06/11
Posts: 3,889
Re: Psilocybe silvatica [Re: RuralAnomaly]
    #19405751 - 01/11/14 08:45 PM (10 years, 1 month ago)

Thank you for the warmth, RuralAnomaly. I'm glad you've found it of use.


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Invisiblemaynardjameskeenan
The white stipes
Male

Folding@home Statistics
Registered: 11/11/10
Posts: 16,391
Loc: 'Merica
Trusted Identifier
Re: Psilocybe silvatica [Re: The Lightning]
    #19418775 - 01/14/14 06:21 PM (10 years, 1 month ago)

I think I may have found some Basidium.
I know pretty much nothing about what I'm doing so please go easy on me.





I think these might be cell walls or something.



You can see the nucleus of these spores.





So I just cut a super small piece of the gill off and kept cutting it into smaller section until I could do it no more.
I mounted(?) it in water and I noticed the spores were shooting off the gill when I put it under the scope, never realized they did that.

Anyway, I sent out sample to Joust, Alonso, and one other person Alan suggested yesterday.
So for now you can look at my shitty microscopy. It's hard to see anything other than spore without stain I think.


Edited by maynardjameskeenan (01/14/14 06:27 PM)


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Offlinesuchen
Once and Future Noob
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Registered: 06/28/11
Posts: 8,841
Loc: Shangri-la
Last seen: 3 years, 2 months
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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19418924 - 01/14/14 06:44 PM (10 years, 1 month ago)

Looks like a mixture of basidia and basidioles in the first two photos. Nice! A lot of the "nuclei" you are seeing may be oil droplets.


--------------------
Rod Tulloss said:

The bulb is the bulb.

The volva is the volva.

They have a very long term realtionship, but they’re “just friends.”


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Invisiblemaynardjameskeenan
The white stipes
Male

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Registered: 11/11/10
Posts: 16,391
Loc: 'Merica
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Re: Psilocybe silvatica [Re: suchen]
    #19419023 - 01/14/14 07:01 PM (10 years, 1 month ago)

Quote:

suchen said:
Looks like a mixture of basidia and basidioles in the first two photos. Nice! A lot of the "nuclei" you are seeing may be oil droplets.



I didn't use any oil, I just put a small water droplet under the coverslip.


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May you be filled with loving kindness.
May you be well.
May you be peaceful and at ease.
May you be happy.



AMU Q&A


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Offlinesuchen
Once and Future Noob
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Registered: 06/28/11
Posts: 8,841
Loc: Shangri-la
Last seen: 3 years, 2 months
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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19419034 - 01/14/14 07:03 PM (10 years, 1 month ago)

The spores have oil droplets inside them naturally. Not sure whether they are actually "oil", but that is what they are called.


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Rod Tulloss said:

The bulb is the bulb.

The volva is the volva.

They have a very long term realtionship, but they’re “just friends.”


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Invisiblemaynardjameskeenan
The white stipes
Male

Folding@home Statistics
Registered: 11/11/10
Posts: 16,391
Loc: 'Merica
Trusted Identifier
Re: Psilocybe silvatica [Re: suchen]
    #19419040 - 01/14/14 07:03 PM (10 years, 1 month ago)

Quote:

suchen said:
The spores have oil droplets inside them naturally. Not sure whether they are actually "oil", but that is what they are called.



O okay, I didn't know that.
:dunce:


--------------------
May you be filled with loving kindness.
May you be well.
May you be peaceful and at ease.
May you be happy.



AMU Q&A


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Offlinesuchen
Once and Future Noob
Male User Gallery


Registered: 06/28/11
Posts: 8,841
Loc: Shangri-la
Last seen: 3 years, 2 months
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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19419061 - 01/14/14 07:08 PM (10 years, 1 month ago)

Those big blogs may be nuclei though like you said. I am unsure.


--------------------
Rod Tulloss said:

The bulb is the bulb.

The volva is the volva.

They have a very long term realtionship, but they’re “just friends.”


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Invisiblemaynardjameskeenan
The white stipes
Male

Folding@home Statistics
Registered: 11/11/10
Posts: 16,391
Loc: 'Merica
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Re: Psilocybe silvatica [Re: suchen]
    #19419086 - 01/14/14 07:12 PM (10 years, 1 month ago)

Is it normal for the Basidium to eject spore when you put water on the gills?
It was really cool looking, I wish I had gotten a video of it, it looked kinda like mini-fireworks.


--------------------
May you be filled with loving kindness.
May you be well.
May you be peaceful and at ease.
May you be happy.



AMU Q&A


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OfflineAlan RockefellerM
Mycologist
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Registered: 03/10/07
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Last seen: 3 days, 14 hours
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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19420043 - 01/14/14 10:23 PM (10 years, 1 month ago)

Quote:

maynardjameskeenan said:
Is it normal for the Basidium to eject spore when you put water on the gills?






No, I have never see basidia eject spores.

The spores look about the right shape for P. silvatia, P. pelliculosa has longer spores and P. semiinconspicua has wider spores.


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Offlinenomadbrad
Oregrownian
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Registered: 10/23/06
Posts: 1,160
Loc: Pacific NW
Last seen: 5 years, 4 months
Re: Psilocybe silvatica [Re: Alan Rockefeller]
    #19420068 - 01/14/14 10:28 PM (10 years, 1 month ago)

Quote:

Alan Rockefeller said:
Quote:

maynardjameskeenan said:
Is it normal for the Basidium to eject spore when you put water on the gills?






No, I have never see basidia eject spores.

The spores look about the right shape for P. silvatia, P. pelliculosa has longer spores and P. semiinconspicua has wider spores.





:popcorn:  I can barely stand the anticipation :rotfl:


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Invisiblemaynardjameskeenan
The white stipes
Male

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Registered: 11/11/10
Posts: 16,391
Loc: 'Merica
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Re: Psilocybe silvatica [Re: nomadbrad]
    #19420093 - 01/14/14 10:34 PM (10 years, 1 month ago)

Quote:

nomadbrad said:
Quote:

Alan Rockefeller said:
Quote:

maynardjameskeenan said:
Is it normal for the Basidium to eject spore when you put water on the gills?






No, I have never see basidia eject spores.

The spores look about the right shape for P. silvatia, P. pelliculosa has longer spores and P. semiinconspicua has wider spores.





:popcorn:  I can barely stand the anticipation :rotfl:



:justcantwait:


--------------------
May you be filled with loving kindness.
May you be well.
May you be peaceful and at ease.
May you be happy.



AMU Q&A


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Invisiblemaynardjameskeenan
The white stipes
Male

Folding@home Statistics
Registered: 11/11/10
Posts: 16,391
Loc: 'Merica
Trusted Identifier
Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19423781 - 01/15/14 04:58 PM (10 years, 1 month ago)

I've noticed that if I crush the sample under the coverslip I can see things much better.
cystidia maybe :shrug:






The basidium look like they all have two sterigma



I'll try to get the micrometer and piximetre setup so I can start measuring stuff.


--------------------
May you be filled with loving kindness.
May you be well.
May you be peaceful and at ease.
May you be happy.



AMU Q&A


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InvisiblerhizoRider
Mycorrhizally expanding
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Registered: 12/24/13
Posts: 2,160
Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19423875 - 01/15/14 05:15 PM (10 years, 1 month ago)

:popcorn:


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Offlinesuchen
Once and Future Noob
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Registered: 06/28/11
Posts: 8,841
Loc: Shangri-la
Last seen: 3 years, 2 months
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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19424037 - 01/15/14 05:45 PM (10 years, 1 month ago)

Quote:

maynardjameskeenan said:
I've noticed that if I crush the sample under the coverslip I can see things much better.





:emotionalmoment:

They grow up so fast.


--------------------
Rod Tulloss said:

The bulb is the bulb.

The volva is the volva.

They have a very long term realtionship, but they’re “just friends.”


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OfflineAlan RockefellerM
Mycologist
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Registered: 03/10/07
Posts: 48,312
Last seen: 3 days, 14 hours
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Re: Psilocybe silvatica [Re: maynardjameskeenan]
    #19424139 - 01/15/14 06:12 PM (10 years, 1 month ago)

Quote:

maynardjameskeenan said:
I've noticed that if I crush the sample under the coverslip I can see things much better.
cystidia maybe :shrug:






The cystidia maybe are basidia and basidioles. 
I see both two and 4 spored basidia in your last photo.


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Offlinenomadbrad
Oregrownian
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Registered: 10/23/06
Posts: 1,160
Loc: Pacific NW
Last seen: 5 years, 4 months
Re: Psilocybe silvatica [Re: Alan Rockefeller]
    #19439027 - 01/18/14 05:59 PM (10 years, 1 month ago)

These were the photos I took of this species when I first found them.  I knew they were strange and interesting, luckily I showed MJK and he assured me that we needed to document them and have them looked at.

They look like a different species in every pic.



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InvisibleJoust
Mycotographer
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Registered: 10/13/11
Posts: 13,392
Loc: WA Flag
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Re: Psilocybe silvatica [Re: nomadbrad]
    #19440268 - 01/18/14 11:14 PM (10 years, 1 month ago)

beautiful!


--------------------
~~~~~~***Psilocybin Mushrooms***~~~~~~
_________A Practical Guide To Psilocybin Mushrooms_________

:sporedrop:                      "Think about the species, not your scale". -NeoSporen                      :sporedrop:

"Mr. Joust, I see you don't actually partake in the psilocin, but it looks like it may partake in you!" -Gojira


       


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Offlinepsylosymonreturns
aka Gym Sporrison
Male


Registered: 10/16/09
Posts: 13,948
Loc: Mos Eisley,
Last seen: 3 years, 6 months
Re: Psilocybe silvatica [Re: Joust]
    #19440288 - 01/18/14 11:19 PM (10 years, 1 month ago)

Super cool!! Thanks for sharing more pics! I cant wait to see some results .


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