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OnlinecronicrMFacebook
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Registered: 08/07/11
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Re: Can I inoculate grains that have already been inoculated and failed? [Re: vikingsc]
    #19450832 - 01/21/14 12:23 AM (10 years, 11 months ago)

i love otto's tek but it's dated, try TL'S it's much easier:rockon:


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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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Offlinestrike
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Re: Can I inoculate grains that have already been inoculated and failed? [Re: cronicr]
    #19471547 - 01/25/14 11:20 AM (10 years, 11 months ago)

I'm researching more and more on this site for possible options. Agar...maybe liquid culture, the BRF Agar substitute Vikingsc suggested...

UPDATE: I have clean Mex A mycelium growth in 8 out of 8 of my re-inoculated Rye Grain jars after 7 days! I'm staying optimistic for no contams hopefully, and continuing the gathering of information on this site! Thanks again everyone

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Re: Can I inoculate grains that have already been inoculated and failed? [Re: cronicr]
    #19471580 - 01/25/14 11:32 AM (10 years, 11 months ago)

How do I find these teks? I've just been surfing around the site, I don't know how to specifically find "TL'S" tek

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Re: Can I inoculate grains that have already been inoculated and failed? [Re: strike]
    #19472545 - 01/25/14 03:04 PM (10 years, 11 months ago)



--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

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Offlinestrike
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Re: Can I inoculate grains that have already been inoculated and failed? [Re: cronicr]
    #19473799 - 01/25/14 08:01 PM (10 years, 11 months ago)

Much thanks friend!

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Offlineafk77
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Re: Can I inoculate grains that have already been inoculated and failed? [Re: strike]
    #19475201 - 01/26/14 04:21 AM (10 years, 11 months ago)

you might be able to PC it again and then re-nock

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Offlineolz1010
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Re: Can I inoculate grains that have already been inoculated and failed? [Re: strike]
    #19475617 - 01/26/14 07:37 AM (10 years, 11 months ago)

I had some 1/2 pint jars of wbs inoculated with a gt spore syringe. 4 weeks in the incubator and no signs of life, my lids have an injection port, wiped it with alcohol and shot up with an A+ LC syringe i made. Fully colonised in 7 days. And if any gt spores survive then happy days, mixed crop  :smile:


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Cube collection: PF Red Spore, GT, AA+, B+, Ecuador, Orissa India, Thai, Huautla, Ps. Cyanescens.




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Offlinevikingsc
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Re: Can I inoculate grains that have already been inoculated and failed? [Re: afk77]
    #19476066 - 01/26/14 10:24 AM (10 years, 11 months ago)

Quote:

afk77 said:
you might be able to PC it again and then re-nock



Too late. He re-nocc'ed his jars a week ago.

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Offlinenumnum59
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Registered: 12/07/11
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Re: Can I inoculate grains that have already been inoculated and failed? [Re: vikingsc]
    #19476996 - 01/26/14 01:59 PM (10 years, 11 months ago)

hows the growth on these things

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Offlinestrike
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Re: Can I inoculate grains that have already been inoculated and failed? [Re: numnum59]
    #19504219 - 02/01/14 12:07 AM (10 years, 11 months ago)

They are growing reasonably slow, but there is SURE progress. I wish I could provide pictures. I'll try to get some pictures this coming weekend. The mycelium is not particularly strong/thick, but not exactly super thing and wispy. Kinda in between. They still have a long way to go, hopefully they don't stall. No signs of contams at all so far. Its been almost 2 weeks.

The main thing to decide now is when/if to shake them. Pictures [hopefully] soon to come!

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