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Offlinekaptanoblivious
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Registered: 11/24/13
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Last seen: 10 years, 5 days
Re: Another first time grow [Re: kaptanoblivious]
    #19409537 - 01/12/14 06:19 PM (10 years, 1 month ago)

Another update and looking for some advice on starting up a second grow.

I currently have:
2 MS Liquid cultures (Cambodian and Z-strain, ~500mL)
10 agar isolates from spores (4 cambodian, 4 Z-strain, 2 gulf coast)

I would like to step up my last effort, since my pint BRF cakes from LC were an ill-advised venture, due to the jar size and moisture content problems. They did grow, and I spawned them to coco/verm monotub 1-2 days ago, but it was not ideal and I'm worried about contamination since some of the jars weren't colonized in the center.

To that end, I now have some good WBS and 15 quart jars and want to do 2 monotubs for next time.

My question here is, which is best to continue with? I have since realized that LCs turn a lot of experienced growers off for good reason. I have these isolates on agar, but how do I know how good they are without doing a grow and testing them? Should I do one BRF cake with each isolate to see which is best? Just pick one and do agar wedges to WBS jars?


Some pictures, sorry about the quality guys.

Some cambodian and z-strain isolates on agar:


An interesting difference in myc between dextrose agar and sabouraud agar:

These are the same isolate, same conditions. I was just testing recipes at this point. The SBA agar isolates grew much thicker (grew high above the agar) but expanded slower.

I isolated by dropping spore solution on plates and streaking out:

These is from 2cc of a gulf coast syringe, with very few viable spores. My first plate of 1 drop of spores produced nothing. This is probably why my LC of this was a failure.

My current monotub on day 1 of colonizing:


This was mostly based on Damion5050's tek.

End of update. Any input on where you would go next appreciated.


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Edited by kaptanoblivious (01/12/14 06:35 PM)


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Offlinekaptanoblivious
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Registered: 11/24/13
Posts: 117
Last seen: 10 years, 5 days
Re: Another first time grow [Re: kaptanoblivious]
    #19409966 - 01/12/14 08:15 PM (10 years, 1 month ago)

Okay, so after reading through old threads for a few hours, my plan moving forward is to use my Z-strain culture to noc up the quart jars of WBS. I will also use my pint jars half-filled for BRF cakes to test a dozen isolates. I will keep updating in this thread with my mobotub progress, along with the start of my next projects. :thumbup:


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OfflinecronicrFacebook
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Re: Another first time grow [Re: kaptanoblivious]
    #19409994 - 01/12/14 08:21 PM (10 years, 1 month ago)

agar wedge to cakes with proper filters are best suited for iso tests


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineHappy Littletree
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Registered: 01/02/14
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Re: Another first time grow [Re: kaptanoblivious]
    #19410087 - 01/12/14 08:40 PM (10 years, 1 month ago)

Great job.. You took off flying for your first grow

Looking forward to updates!


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Offlinekaptanoblivious
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Registered: 11/24/13
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Re: Another first time grow [Re: Happy Littletree]
    #19424281 - 01/15/14 06:50 PM (10 years, 1 month ago)

4 days after putting spawn to bulk. I may have left the crumbled cakes a little too chunky, but It's Alive...



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OfflinecronicrFacebook
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Re: Another first time grow [Re: kaptanoblivious]
    #19424370 - 01/15/14 07:13 PM (10 years, 1 month ago)

:frankly:
:laugh2:i'm just kidding man looking good


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlinekaptanoblivious
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Registered: 11/24/13
Posts: 117
Last seen: 10 years, 5 days
Re: Another first time grow [Re: cronicr]
    #19424380 - 01/15/14 07:16 PM (10 years, 1 month ago)

:cryaboutit:

Edit: just saw your blacked out text.
:knockmeonmyass:


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Edited by kaptanoblivious (01/15/14 07:18 PM)


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OfflinecronicrFacebook
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Re: Another first time grow [Re: kaptanoblivious]
    #19424410 - 01/15/14 07:22 PM (10 years, 1 month ago)

:laugh2::rockon:


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlinekaptanoblivious
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Registered: 11/24/13
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Last seen: 10 years, 5 days
Re: Another first time grow [Re: cronicr]
    #19440884 - 01/19/14 02:10 AM (10 years, 1 month ago)

Just got done with setting up the next round of jars. Man this can be a lot of work! Made everything with wild bird seed following Franks tek.

8 WBS quart jars of Z strain (multispore LC)
7 WBS quart jars of Cambodian (multispore LC)
12 WBS pint jars of various Z strain, Cambodian, and Gulf Coast isolates (agar wedges)

I had planned on using the pint jars for BRF cakes, but I made way too much WBS that I didn't want to waste. I'm not sure exactly what I'm going to do since I have heard that WBS cakes aren't the best. I think I will try to separate each isolate in coir/verm trays in a monotub or two.

I also just put my first monotub into fruiting, since it has been fully colonized for a day or two. I don't see any contaminates, but it didn't exactly smell right when I briefly opened it for adjusting the polyfill :frown:. Not that I have experience with what it should smell like.


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OfflinecronicrFacebook
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Re: Another first time grow [Re: kaptanoblivious]
    #19440896 - 01/19/14 02:13 AM (10 years, 1 month ago)

should smell like mushrooms, snap a pic:thumbup: and fruit it already:wink:


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflinecronicrFacebook
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Re: Another first time grow [Re: cronicr]
    #19440899 - 01/19/14 02:14 AM (10 years, 1 month ago)

:frankly:


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlinekaptanoblivious
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Registered: 11/24/13
Posts: 117
Last seen: 10 years, 5 days
Re: Another first time grow [Re: cronicr]
    #19440947 - 01/19/14 02:38 AM (10 years, 1 month ago)

Quote:

cronicr said:
should smell like mushrooms, snap a pic:thumbup: and fruit it already;)





OK OK here is a picture of my first crappy monotub attempt. I'm guessing its either contammed to hell or I jumped the gun with starting FAE before that last little bit was colonized. Do you give a damn yet? :crazy:





This first time was more a learning experience anyway, I'd be surprised if I get any fruits from this thing.


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Edited by kaptanoblivious (01/19/14 02:54 AM)


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Invisiblemushmagic
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Registered: 03/21/12
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Re: Another first time grow [Re: kaptanoblivious]
    #19441158 - 01/19/14 04:51 AM (10 years, 1 month ago)

You did jump the gun just a little bit but you should still definitely get some fruits off it. Coirs pretty contam resistant compared to most subs so you'll fare a little better with it too.


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Offlinekaptanoblivious
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Registered: 11/24/13
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Last seen: 10 years, 5 days
Re: Another first time grow [Re: mushmagic]
    #19445195 - 01/19/14 10:55 PM (10 years, 1 month ago)

Thanks for the input mushmagic. The tub is continuing to finish colonizing, even though I gave it FAE too soon. Hopefully I can get a first flush out of it. I'm thinking the weird smell is because I cleaned the inside of the tub out with vinegar right before adding everything. 

:woooaaahhh:

I probably should have rinsed that off with something.

Is it normal to see droplets of water all over the inside of my monotub, and also some on top my the mycelium? I don't usually see monotubs on here that look like that. Does this mean my coir/verm was beyond field capacity? I did the squeeze test as recommended, and only a few drops of water came out. I did dunk my BRF cakes before I used them though :shrug:


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Invisiblemushmagic
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Re: Another first time grow [Re: kaptanoblivious]
    #19445394 - 01/19/14 11:38 PM (10 years, 1 month ago)

No Prob, I actually thought you completely introduced it to fruiting but if you just opened it to snap a quick pic it should have no problem finishing colonizing anyway.

The water droplets on the side of tub is just condensation from the temp differential of the outside and inside of the tub. It's also normal for some water droplets to fall on top of the sub, no worries. If it's excessive pooling then you can dab it up with a paper towel when it comes time to initiate fruiting.

If only a few drops came out when squeezing then it should be at field capacity.


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Trade list in journal (partially under construction; more to be added)


Don't judge a man by what kinda shoes he in, judge a man on where that man's shoes been.


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Offlinetwistedty
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Re: Another first time grow [Re: mushmagic]
    #19445915 - 01/20/14 03:07 AM (10 years, 1 month ago)

looks like trich to me


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Offlinekaptanoblivious
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Registered: 11/24/13
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Last seen: 10 years, 5 days
Re: Another first time grow [Re: twistedty]
    #19469284 - 01/24/14 08:04 PM (10 years, 1 month ago)

you're right twisted i think its contaminated to some extent. There are no visible signs of anything but myc to my inexperienced eyes, but it smells kind of sweet. Since its my first time I'm going to let it go and see if I can get anything at all out of it. I'd like to practice taking a print, cloning from fruits, etc. Is this a terrible idea?

I assume this is a bacterial contamination carried over from my overly wet BRF cakes. :shrug: They smelled only like mushies. Oh well, it was a first attempt.

at least all of my WBS jars are doing well for a second attempt. I think I will pasteurize properly next time before spawning to bulk too.


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Edited by kaptanoblivious (01/25/14 07:11 PM)


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Offlinekaptanoblivious
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Registered: 11/24/13
Posts: 117
Last seen: 10 years, 5 days
Re: Another first time grow [Re: kaptanoblivious]
    #19473587 - 01/25/14 07:15 PM (10 years, 30 days ago)

Just an update on what these tubs look like. I saw another user posting similar looking tubs today, and others were saying they looked good, so this gives me more hope than I had.

Here are some update pictures


Its been about a week since I started FAE

There are a few yellow/tan spots, that I couldn't get a good picture of. Do these look happy?


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Edited by kaptanoblivious (01/25/14 09:32 PM)


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OfflinecronicrFacebook
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Re: Another first time grow [Re: kaptanoblivious]
    #19474188 - 01/25/14 09:47 PM (10 years, 30 days ago)

:thumbup:


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlinekaptanoblivious
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Registered: 11/24/13
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Last seen: 10 years, 5 days
Re: Another first time grow [Re: cronicr]
    #19474200 - 01/25/14 09:50 PM (10 years, 30 days ago)

:zomgzomgzomg: cronicr gave me a thumbs up


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