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InvisibleImperfect Iam
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Syringe water still sterile?
    #19407964 - 01/12/14 11:36 AM (10 years, 1 month ago)

I sucked up 3 syringes w/ boiling  water to spray over reishi covered agar about a week ago.  I wonder if they should be good the caps on and no one has touched them?


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All you touch, and all you see, is all your life will ever be- Pink Floyd

Life is what happens while you're busy making other plans- John Lennon


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OfflineJeff
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Re: Syringe water still sterile? [Re: Imperfect Iam]
    #19408652 - 01/12/14 02:43 PM (10 years, 1 month ago)

Boiling is not sterilizing cc1. 
You are better off emptying the syringes.  Throw the syringes, taken apart, wrapped in foil and a mason jar full of water in a pc.  Then you will know for sure that it is sterile.


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Myco-tek


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InvisibleImperfect Iam
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Re: Syringe water still sterile? [Re: Jeff]
    #19408711 - 01/12/14 02:59 PM (10 years, 1 month ago)

How long in PC?


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All you touch, and all you see, is all your life will ever be- Pink Floyd

Life is what happens while you're busy making other plans- John Lennon


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Re: Syringe water still sterile? [Re: Imperfect Iam]
    #19408743 - 01/12/14 03:06 PM (10 years, 1 month ago)

45 minutes for a pint jar of water.  Place the lid seal up and tighten the ring.  The syringe can sit on top of the jar.


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Myco-tek


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InvisibleImperfect Iam
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Re: Syringe water still sterile? [Re: Jeff]
    #19408824 - 01/12/14 03:28 PM (10 years, 1 month ago)

Thank you feff, I know better than to call that water sterile.
Hey you gotta check out the bag and I'm doing 2 more ATM, I think these new ones I just want to try to grow stemless conks, I'm not sure yet, I wanted you to see pics, the first is of Forresters and my grain at 7days, second is of the bag u helped me with moisture top view,  third one top, can you see the pins? The next is a bottom shot, and the next is just a side view, and the last is a five galloon bucket with about 4inches to substrate from the top!


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All you touch, and all you see, is all your life will ever be- Pink Floyd

Life is what happens while you're busy making other plans- John Lennon


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OfflineKizzle
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Re: Syringe water still sterile? [Re: Imperfect Iam]
    #19409047 - 01/12/14 04:27 PM (10 years, 1 month ago)

There's no need to PC a syringe. Boiling the syringe and sucking up boiling water is sufficient to create a syringe of sterile water. A lot of people don't even boil the syringe and repeatedly suck in and squirt out boiling water to sterilize the inside.


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InvisibleGanzig
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Re: Syringe water still sterile? [Re: Kizzle]
    #19409282 - 01/12/14 05:21 PM (10 years, 1 month ago)

I have done the boiling water technique multiple times with no contams ever.


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Re: Syringe water still sterile? [Re: Ganzig]
    #19409298 - 01/12/14 05:24 PM (10 years, 1 month ago)

:shrug: 
I just like to make sure things are sterile. :smile:.


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Myco-tek


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InvisibleGanzig
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Re: Syringe water still sterile? [Re: Jeff]
    #19409403 - 01/12/14 05:48 PM (10 years, 1 month ago)

It is a safe bet. :cool:


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Re: Syringe water still sterile? [Re: Ganzig]
    #19409414 - 01/12/14 05:50 PM (10 years, 1 month ago)

As to cc's question of using them now....I still wouldn't.


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Myco-tek


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InvisibleGanzig
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Re: Syringe water still sterile? [Re: Jeff]
    #19409431 - 01/12/14 05:54 PM (10 years, 1 month ago)

Yeah, boiling water is pretty easy. I would do it again. :lol:


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I must keep reminding myself of this.
I must keep reminding myself of this. I must keep reminding myself of this.
I must keep reminding myself of this.


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OfflineMAIA
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Re: Syringe water still sterile? [Re: Imperfect Iam]
    #19412427 - 01/13/14 01:20 PM (10 years, 1 month ago)

Fastest way to reuse a syringe is to fill the syringe up with 90% alcohol and then refill with distilled water. Boiling also works ok but PCing the syringe is safer.


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Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
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InvisibleImperfect Iam
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Re: Syringe water still sterile? [Re: MAIA]
    #19525439 - 02/05/14 01:23 PM (10 years, 19 days ago)

Hey thanks everyone for the feedback! Yeah why waste the time and effort if you aren't going to do it right, this is how I can tell I am advancing in my cultivation tech. compared to when I was a super noob, still am and always will be a noob as I read in someone's sig, but just not super greenhorn!


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All you touch, and all you see, is all your life will ever be- Pink Floyd

Life is what happens while you're busy making other plans- John Lennon


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