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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab
#19408091 - 01/12/14 12:11 PM (10 years, 1 month ago) |
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In honor of BlueConfusion I am starting my spore to spore grow log here. Click the first sentence to go back to the origin thread blue started.
I've sent out a couple creeper spore swabs the same of which ones I have used to start this grow.
You're going to need a few things but if you're tagging along you probably already have what you need. I'll leave you to your own devices and coach people along the way as to how to get the most out of what they have. You can choose your own agar recipes etc... If you need help send me a PM or post here though, don't be shy. Please get into agar it's fun and easy.
Here's two dishes, I chose these specifically since they show contams. The dish on the left says C.s.1.(c) this is my short hand for Creeper, swab, first dish, and plate series C. I think I went up to (f) for this grow which means I used the swab on A-F(6) dishes. The dish on the right says C.s.2.(d) so Creeper, swab, 2nd dish(agar from first dish to a new plate) and then plate series D.
I'll be taking the plate on the left and making C.s.2.(c) today and then taking the dish on the right and making C.s.3.(d)
I didn't make all 6 origin dishes at the same time that's why the plate on the left is less grown out than the plate on the right. I did D E F one day and then A B C a few days later with a different swab.

The reason for showing you these contamed dishes is to show new growers specifically that contams are not scary on dishes and everyone gets them from time to time, Well maybe not everyone, but I use a SAB and I'm very stoned when I do my dish work so sue me. Anyway I'll be showing that you can effectively transfer away from contams and obtain a clean culture successfully.
I have agar cooking right now so the next round of dishes should be made some time tonight.
After that they'll be put to Rye grain in quart jars and then spawned to coir+verm that will be properly pasteurized.
My agar recipe is 500ml H2O to 10G agar to 10G ELME(extra light malt extract) any light spray-malt extract will work. Spray malt is the dry powder, you'll want that not the liquid most likely unless you have some way to store it. Spray malt is EXTREMELY hygroscopic so you'll learn how to deal with that quickly, word of advice don't spill it on your scale 
I'll post the links to my update posts below so it's easy to follow from this post
Here's the first update 1/14/14 Transfer from origin dishes, dishes on their 2nd and 3rd transfer from the spore swab
1/16/14 Just for fun update
1/17/14 the next set of transfers dishes now on 3rd and 4th transfers
1/23/14 Update on those last transfers, Very cold in here slow growth.
1/25/14 The next set of transfers, dishes now on their 4th and 5th transfers
2/3/14 transfers and update pictures, dishes now on their 5th and 6th transfers
2/6/14 picture update of transfers
2/10/14 picture update
2/14/14 yet another picture update, transfers made IE 6th and 7th transfers, not pictured
2/20/14 update showing 6th and 7th transfer plates after 6 days
2/24/14 6&7th transfer plates that were inoculated on 2/14/14 ten days later on the 24th
Edited by Trusted cuItivator (02/24/14 06:57 PM)
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: bodhisatta]
#19408169 - 01/12/14 12:32 PM (10 years, 1 month ago) |
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: cronicr]
#19408228 - 01/12/14 12:49 PM (10 years, 1 month ago) |
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i'm pretty busy prepping shit today but i'll keep up with ya, probly start with a clone i get from my grow along with blue
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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KnownPown
Fathead Shroomer



Registered: 10/27/12
Posts: 219
Loc: Washington State
Last seen: 6 years, 6 months
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: cronicr]
#19408270 - 01/12/14 12:59 PM (10 years, 1 month ago) |
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Yay another grow along thread (: I like your setup so far, andI hope we get some more people in on this.
I just started agar for my first time too, and have some B+ spores growing out right now. Going to make some transfers today so I'll be right behind you =)


I've also got some clones an agar, but they all look pretty contamed, maybe someone can help me out with what I should do? =)
  
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1down5up
Social Ninja



Registered: 11/04/13
Posts: 1,743
Last seen: 1 year, 3 months
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: cronicr]
#19408277 - 01/12/14 01:02 PM (10 years, 1 month ago) |
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I'll be watching for sure, my next grow after this round of cakes will be very similar to what you have going on. I have one question, does agar always need some sort of nutrient added to it when you use it, or can you just use the straight powder with water? I've seen a few peep on here add things for some benefit...but do you have to?
-------------------- ~~Everything is relative~~ A Simplified Overview of Mushroom Cultivation Strategies - RR says - EvilMushroom666's Take on BRF Cakes - Frank's list of goodies - Cronicr's Goodies No one is placed higher than another no matter race or creed or gender, we must teach forgiveness and compassion for all life. J.L.
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blueconfusion
Strangest


Registered: 12/14/12
Posts: 1,727
Last seen: 1 day, 3 hours
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: 1down5up]
#19408312 - 01/12/14 01:12 PM (10 years, 1 month ago) |
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I'm here as soon as I get something to print
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: blueconfusion]
#19408346 - 01/12/14 01:22 PM (10 years, 1 month ago) |
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Quote:
I've also got some clones an agar, but they all look pretty contamed, maybe someone can help me out with what I should do? =)
It's a bit hard to see, The light shining at the camera from the back of the dishes is throwing off the exposure and making the mycelium hard to see, In practice though you would just be really carefully trying to take clean mycelium and place it onto a new plate, it might take more than one try and transfer.
Quote:
1down5up said: I'll be watching for sure, my next grow after this round of cakes will be very similar to what you have going on. I have one question, does agar always need some sort of nutrient added to it when you use it, or can you just use the straight powder with water? I've seen a few peep on here add things for some benefit...but do you have to?
I bet things would germinate on just agar+water but they wouldn't do much after that. You do need some sort of nutrition like dextrose or malt extract or cat food etc... Frankhorrigan has an agar journal it should be easy to find if you just type frankhorrigan agar into the top of the screen.
Quote:
blueconfusion said: I'm here as soon as I get something to print
Awesome.
and Hello chronicr.
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: bodhisatta] 1
#19408398 - 01/12/14 01:37 PM (10 years, 1 month ago) |
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Just poured up 13 dishes, weird number but I have re-usable glass dishes so I had to clean some out to sterilize them with my agar earlier this morning. These dishes will take some time to cool down and solidify then I can start doing second and third transfers respectively. Most of the time I'm letting my agar cool and sit for a few days before inoculating it just to make sure the plates are clean. I would suggest this if you're starting out. I've stopped having contams on poured plates, there always clean now. I do get some contams doing the actual transfers but that's usually my bad accidentally touching the handle of a tool to a bad surface or doing something stupid etc... Usually I'll have a good idea what dishes will show a contam because of some jankey movement I made in the SAB.
I've had some micropore tape laying around it's 1 inch wide and I never used it in this hobby ever, I bought it when I was doing PF tek and never used it since. I'm now using it to wrap my dishes and it works great. Peals off just fine on glass dishes with no residue left over, it's micropore so it's doing it's job keeping the dishes clean and safe to handle, and it's super easy to tear inside of the sab.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: bodhisatta]
#19408625 - 01/12/14 02:36 PM (10 years, 1 month ago) |
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i'll post some pics tonight, got a whole whack of shit on the go
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: cronicr]
#19408667 - 01/12/14 02:47 PM (10 years, 1 month ago) |
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Quote:
cronicr said: i'll post some pics tonight, got a whole whack of shit on the go

I'm putting that dish with the mean green out to 3 more dishes for this thread in specific so that we can follow the progress from that plate up until a good clean culture and grow out. I'll just call those dishes C.s.3t(t for trich) and I'll drop the series designation (d). I'll also put the plate that was on the left with that small spot of bacterial onto 3 new dishes and call those C.s.2b(b for bacterial) and also drop the letter designation (c) for the series too. Those are the dishes that will be part of this grow thread then just to keep it neat and to the point. So soon we will have 6 dishes 3 of them being a 2nd transfer away from bacteria and then 3 of them being a 3rd transfer and away from the green mold. I'll attempt to get isolate cultures and fruit them out for everyone's amusement.
So C.s.2.(d) is turning into 3 plates all named C.s.3t C.s.1.(c) is turning into 3 plates all named C.s.2b

The plate with trich(right) is having 3 small slivers taken from the 12-1 position in the picture. It's convenient that it's the best growth and also 180 from the green.
The bacterial plate(left) is having sections taken from 2,4 and 6 because that's what I felt like.
Edited by Trusted cuItivator (01/12/14 02:55 PM)
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: bodhisatta]
#19408672 - 01/12/14 02:48 PM (10 years, 1 month ago) |
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i've been the luckiest fucker alivelately, used all kinds of wild dirty prints with no contams....knock on wood
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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J. Jack Flash
stranger than ever.


Registered: 11/20/13
Posts: 1,500
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: cronicr]
#19408832 - 01/12/14 03:29 PM (10 years, 1 month ago) |
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well how 'bout that? i just made my first transfers this very day... i think one of the donor plates even has a contaminant. let's do this.
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the j stands for jesus.2020 new years grow along
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,338
Loc: Dept of know what I'm say...
Last seen: 16 hours, 8 minutes
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: J. Jack Flash]
#19408849 - 01/12/14 03:33 PM (10 years, 1 month ago) |
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I got a pe on its way to me right now
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: Stromrider]
#19409033 - 01/12/14 04:23 PM (10 years, 1 month ago) |
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I'm in with a whole pile of stuff that cronicr told me did not qualify for blues thread got some PE clones, a few other clones, and a couple oddities Plus a few other ms projects in various stages from plates to going into fruiting tonight.
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rawrrock
booger bean



Registered: 08/22/13
Posts: 121
Loc: Cold place!
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: Pastywhyte]
#19409131 - 01/12/14 04:47 PM (10 years, 1 month ago) |
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Well isn't this just a dandy. I just swabbed a B+ print onto agar. I'm in!
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60G tea trip report.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: rawrrock]
#19409149 - 01/12/14 04:52 PM (10 years, 1 month ago) |
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i'm out, just lost my shit today right after g2g'ing over 30 jars so i'm not expecting things to go good for me right now. i'll keep watching but i'm gonna step aside from all this for awhile
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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rawrrock
booger bean



Registered: 08/22/13
Posts: 121
Loc: Cold place!
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: rawrrock]
#19409152 - 01/12/14 04:52 PM (10 years, 1 month ago) |
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All these grow alongs, I'm going from PF TEK to doing multiple plates of agar, 20+ jars of grain. So busy, it's great!
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60G tea trip report.
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: bodhisatta]
#19409154 - 01/12/14 04:54 PM (10 years, 1 month ago) |
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You should change your thread title. It's confusing to see two almost identically named threads
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J. Jack Flash
stranger than ever.


Registered: 11/20/13
Posts: 1,500
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: cronicr]
#19409156 - 01/12/14 04:54 PM (10 years, 1 month ago) |
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Quote:
cronicr said: i'm out, just lost my shit today right after g2g'ing over 30 jars so i'm not expecting things to go good for me right now. i'll keep watching but i'm gonna step aside from all this for awhile
luckiest fucker alive indeed. sorry man.
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the j stands for jesus.2020 new years grow along
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: J. Jack Flash]
#19409161 - 01/12/14 04:55 PM (10 years, 1 month ago) |
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yeah go figure all my fucking wild prints and steamed jars are kicking ass, my pc'd fucking shit goes south! WTF!
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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