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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: Sgt. Pepper]
#19424875 - 01/15/14 08:57 PM (10 years, 15 days ago) |
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Quote:
Sgt. Pepper said: Just got the plate swabbed! So I just rubbed the q-tip on the plate, I saw nothing visible on the plate though, did I do that right?
Quote:
Just got a pan print courtesy of one of the good guys here. so expect a grow thread follow along for that soon, I'll post a link here for convenience
Assuming you're talking about me, they're actually in vials. There should be about 6 or so cc's per vial. Ones a pan topicalis and the other is RDU. The tropicalis is pretty dark, there are visible spores floating around and stuff. If you're not talking about me, then forget I said that. Lol.
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: cronicr]
#19424898 - 01/15/14 09:02 PM (10 years, 15 days ago) |
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Quote:
cronicr said:
Quote:
Sgt. Pepper said: Just got the plate swabbed! So I just rubbed the q-tip on the plate, I saw nothing visible on the plate though, did I do that right?
Quote:
Just got a pan print courtesy of one of the good guys here. so expect a grow thread follow along for that soon, I'll post a link here for convenience
Assuming you're talking about me, they're actually in vials. There should be about 6 or so cc's per vial. Ones a pan topicalis and the other is RDU. The tropicalis is pretty dark, there are visible spores floating around and stuff. If you're not talking about me, then forget I said that. Lol.

haha Sgt. your have not come yet, soon hopefully.
and yea you surely got spores on the dish, they're microscopic. Keep us updated.
Edited by Trusted cuItivator (01/15/14 09:04 PM)
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Sgt. Pepper



Registered: 06/19/13
Posts: 2,538
Loc: Third Stone From The Sun
Last seen: 1 month, 23 days
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: cronicr]
#19424899 - 01/15/14 09:02 PM (10 years, 15 days ago) |
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Quote:
cronicr said:
Quote:
Sgt. Pepper said: Just got the plate swabbed! So I just rubbed the q-tip on the plate, I saw nothing visible on the plate though, did I do that right?
Quote:
Just got a pan print courtesy of one of the good guys here. so expect a grow thread follow along for that soon, I'll post a link here for convenience
Assuming you're talking about me, they're actually in vials. There should be about 6 or so cc's per vial. Ones a pan topicalis and the other is RDU. The tropicalis is pretty dark, there are visible spores floating around and stuff. If you're not talking about me, then forget I said that. Lol.

Haha. So never mind my last post then.
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: Sgt. Pepper]
#19424977 - 01/15/14 09:20 PM (10 years, 15 days ago) |
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bodhi, got ur swab today (THX AGAIN!!), put it on agar an hour ago. whatever germinates first, KSSS done 2 days ago, or the creeper, i will use for this thread.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,326
Loc: Dept of know what I'm say...
Last seen: 16 hours, 4 minutes
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: cronicr]
#19424995 - 01/15/14 09:24 PM (10 years, 15 days ago) |
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Quote:
cronicr said:
Quote:
Sgt. Pepper said: Just got the plate swabbed! So I just rubbed the q-tip on the plate, I saw nothing visible on the plate though, did I do that right?
Quote:
Just got a pan print courtesy of one of the good guys here. so expect a grow thread follow along for that soon, I'll post a link here for convenience
Assuming you're talking about me, they're actually in vials. There should be about 6 or so cc's per vial. Ones a pan topicalis and the other is RDU. The tropicalis is pretty dark, there are visible spores floating around and stuff. If you're not talking about me, then forget I said that. Lol.

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kratom_redmomd
Kratom Lover



Registered: 07/25/13
Posts: 411
Loc: Hiking Cascadia
Last seen: 4 years, 3 months
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: rawrrock]
#19425835 - 01/16/14 12:53 AM (10 years, 15 days ago) |
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Quote:
rawrrock said:
I swiped probably a week ago... maybe a little less.
Kewl. It's only been a couple of days. I will relax and soak some grains.
-------------------- "If the goal is to ride the bike, why is everyone arguing about which bike to ride? We are all bike riders. Ride your bike." -Unknown If I was going to destroy a nation, first I would destroy its language. -Confusious ¿Hablas Inglés?
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: blindingleaf]
#19427249 - 01/16/14 10:20 AM (10 years, 14 days ago) |
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Quote:
blindingleaf said: bodhi, got ur swab today (THX AGAIN!!), put it on agar an hour ago. whatever germinates first, KSSS done 2 days ago, or the creeper, i will use for this thread.
Awesome
I have a feeling your stuff might come today Sgt.
As some of you know I have glass petri dishes, I was going to clean out some to make room for the transfers for this thread so I put some old colonized ones in boiling water in a pot with the lid on and left it overnight and today I wake up to a nice pot full of carbon, hopefully my dishes don't crack I'll let them cool down slowly
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Sgt. Pepper



Registered: 06/19/13
Posts: 2,538
Loc: Third Stone From The Sun
Last seen: 1 month, 23 days
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: bodhisatta]
#19427353 - 01/16/14 10:43 AM (10 years, 14 days ago) |
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Quote:
I have a feeling your stuff might come today Sgt.
That's when they're do to arrive, what's the tracking say? Edit:Just checked and it says delivered!
Edited by Sgt. Pepper (01/16/14 10:49 AM)
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: Sgt. Pepper]
#19427401 - 01/16/14 10:54 AM (10 years, 14 days ago) |
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Quote:
Sgt. Pepper said:
Quote:
I have a feeling your stuff might come today Sgt.
That's when they're do to arrive, what's the tracking say? Edit:Just checked and it says delivered!
Awesome, I'll investigate either late today or tomorrow. I would have stuff shipped here but my mail service is horrible and half our mailboxes are broken.
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Sgt. Pepper



Registered: 06/19/13
Posts: 2,538
Loc: Third Stone From The Sun
Last seen: 1 month, 23 days
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: bodhisatta]
#19427419 - 01/16/14 10:57 AM (10 years, 14 days ago) |
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Quote:
bodhisatta said:
Quote:
Sgt. Pepper said:
Quote:
I have a feeling your stuff might come today Sgt.
That's when they're do to arrive, what's the tracking say? Edit:Just checked and it says delivered!
Awesome, I'll investigate either late today or tomorrow. I would have stuff shipped here but my mail service is horrible and half our mailboxes are broken.
Great, can you get the box inside since it does have glass vials full of liquid and it would suck if they froze and cracked.
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: Sgt. Pepper]
#19427426 - 01/16/14 10:59 AM (10 years, 14 days ago) |
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Quote:
Sgt. Pepper said:
Quote:
bodhisatta said:
Quote:
Sgt. Pepper said:
Quote:
I have a feeling your stuff might come today Sgt.
That's when they're do to arrive, what's the tracking say? Edit:Just checked and it says delivered!
Awesome, I'll investigate either late today or tomorrow. I would have stuff shipped here but my mail service is horrible and half our mailboxes are broken.
Great, can you get the box inside since it does have glass vials full of liquid and it would suck if they froze and cracked.
Yep I'll send a text to said people.
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Sgt. Pepper



Registered: 06/19/13
Posts: 2,538
Loc: Third Stone From The Sun
Last seen: 1 month, 23 days
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: bodhisatta]
#19427443 - 01/16/14 11:02 AM (10 years, 14 days ago) |
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Awesome, I'll investigate either late today or tomorrow. I would have stuff shipped here but my mail service is horrible and half our mailboxes are broken.
Great, can you get the box inside since it does have glass vials full of liquid and it would suck if they froze and cracked.
Yep I'll send a text to said people.
Perfect, tell me how it all is doing when you can.
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: Sgt. Pepper]
#19427460 - 01/16/14 11:07 AM (10 years, 14 days ago) |
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It's inside a warm place now. I'll probably get it late tonight and update tomorrow.
Edited by Trusted cuItivator (01/16/14 11:20 AM)
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Sgt. Pepper



Registered: 06/19/13
Posts: 2,538
Loc: Third Stone From The Sun
Last seen: 1 month, 23 days
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: bodhisatta]
#19427473 - 01/16/14 11:10 AM (10 years, 14 days ago) |
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Quote:
bodhisatta said: It's inside a warm place now. I'll probably get it late tonight and update tomorrow.
I'm going to edit this post in a little bit(less than 5 min) with a surprise picture though
Hooray! I love pictures!
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: bodhisatta]
#19427503 - 01/16/14 11:18 AM (10 years, 14 days ago) |
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I'll just leave this here.
1/4 inch "casing layer" made with some reserved properly pasteurized coir/verm mix. Applied during spawning. I'll probably put these in the SGFC buried down a little bit
The one on the left will probably come out a bit sour and Belgian The one on the right will probably be a bit darker and German

It's leftover creeper spawn.
Edited by Trusted cuItivator (01/16/14 11:21 AM)
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magickspore



Registered: 12/11/12
Posts: 798
Loc: Center of the universe.
Last seen: 8 months, 4 days
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: bodhisatta]
#19427516 - 01/16/14 11:22 AM (10 years, 14 days ago) |
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Awww you shouldn't have...
Nice I love doing little cups of shrooms.
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: magickspore]
#19427530 - 01/16/14 11:26 AM (10 years, 14 days ago) |
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Giving good karma to my glassware.
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Gorlami



Registered: 08/28/13
Posts: 328
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: bodhisatta]
#19427788 - 01/16/14 12:21 PM (10 years, 14 days ago) |
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Just got a couple of agar plates done. Followed blue's write-up, but with dextrose this time instead of honey. 
Transfered a sector of Orissa and Texas to new plates. Also picked up a pin from a my first monotub, which I discovered Trich in yesterday. I put that on agar as well.
CAN'T WAIT TO GET MY AGAR SKILLS DOWN SO I CAN AVOID CONTAMNS.
Hypothetical question: Let's say my syringe is lightly contaminated, will it show up when I inject a plate? Since the mycelium seems to be quite aggresive, would that hide any contamns on the agar plate?
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Sgt. Pepper



Registered: 06/19/13
Posts: 2,538
Loc: Third Stone From The Sun
Last seen: 1 month, 23 days
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: bodhisatta]
#19427790 - 01/16/14 12:21 PM (10 years, 14 days ago) |
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Quote:
bodhisatta said:

I'll just leave this here.
1/4 inch "casing layer" made with some reserved properly pasteurized coir/verm mix. Applied during spawning. I'll probably put these in the SGFC buried down a little bit
The one on the left will probably come out a bit sour and Belgian The one on the right will probably be a bit darker and German

It's leftover creeper spawn.
I've always thought about doing something like this but I never got around to it. This is the coolest thing ever.
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Wanna Step Up Your Game? Come Grow Along With Bodhisatta! Spore Swab to Spore Swab [Re: Gorlami]
#19427807 - 01/16/14 12:27 PM (10 years, 14 days ago) |
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Quote:
whalechunks said:

Just got a couple of agar plates done. Followed blue's write-up, but with dextrose this time instead of honey. 
Transfered a sector of Orissa and Texas to new plates. Also picked up a pin from a my first monotub, which I discovered Trich in yesterday. I put that on agar as well.
CAN'T WAIT TO GET MY AGAR SKILLS DOWN SO I CAN AVOID CONTAMNS.
Hypothetical question: Let's say my syringe is lightly contaminated, will it show up when I inject a plate? Since the mycelium seems to be quite aggresive, would that hide any contamns on the agar plate?
Bad news on the trich, I had trich pop up on my cased grain thing today but I figured that project wouldn't go well anyway.
Not to sure about your hypothetical, I would imagine the cube mycelium would either 1. over run it and then if you transfer to a new plate you'll be clean 2. show up everywhere and be quite obvious 3. run with the mycelium. If your dish looks clean, give it a transfer and then from there I would use it or continue to transfer if an isolate is what you're after. 2-3 transfers from a MS syringe is pretty good to get a more limited set of genetics and make sure you're clean.
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